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The map label for this gene is paaI [H]

Identifier: 119896593

GI number: 119896593

Start: 327066

End: 327536

Strand: Direct

Name: paaI [H]

Synonym: azo0302

Alternate gene names: 119896593

Gene position: 327066-327536 (Clockwise)

Preceding gene: 119896592

Following gene: 119896594

Centisome position: 7.47

GC content: 65.82

Gene sequence:

>471_bases
ATGAGTGAAGCCGCCTACCGTGACGTCACTGCCGGCCTCGATCCGCAAGCCCTGGCCGAAAAGGTGCGCGACGGCATGTT
CGAACGCGACCAGGCCGCGCGCAGCCTGGCGATGGAGATCACCGCCGTCGGTCCCGGCCGCGCCACCATCGCGATGAAGG
TGCGAGAGGACATGCTGAACGGTTTCCGCATCTGCCACGGCGGTTTCATTACCACCTTGGCCGACACCGCCTTTGCCTAC
GCCTGCAACAGCGGCAACGAGCAGACCGTGGCGTCCGGCATCAGCGTGGATTTCATGGCGCCGGGCAAGCCGGGCGACGT
GCTGACCGCCGAGGCGCAGCAGGTCTTCGAAGCCGGCCGCACCGGCGTGTACGACATCACCGTCACCAACCAGCAGGGCG
AGCTGATCGCGGTCATGCGTGGCAAGTCCTACCGCCTCAAAGGGAGGCCGGTCGTAGAAATACAGGCCTGA

Upstream 100 bases:

>100_bases
GTCGCCCGCGTGCTGGCCAACCTGCGCGAGCACTACGGCGAGGAACGCTACCGCGTGTCGCCGCTGATCCAGCGCAAGAC
CTTCAGTGGAGCCGCATTCC

Downstream 100 bases:

>100_bases
ACGGCCGCCGCCCGGACAACAAAAAACAGCCTGCCGGGCGGCTCCAGAACCAGCAGAGGGACAGAGGAGACAACCATGCC
CATCAACAGCTATGCACCCC

Product: phenylacetic acid degradation protein PaaI

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSEAAYRDVTAGLDPQALAEKVRDGMFERDQAARSLAMEITAVGPGRATIAMKVREDMLNGFRICHGGFITTLADTAFAY
ACNSGNEQTVASGISVDFMAPGKPGDVLTAEAQQVFEAGRTGVYDITVTNQQGELIAVMRGKSYRLKGRPVVEIQA

Sequences:

>Translated_156_residues
MSEAAYRDVTAGLDPQALAEKVRDGMFERDQAARSLAMEITAVGPGRATIAMKVREDMLNGFRICHGGFITTLADTAFAY
ACNSGNEQTVASGISVDFMAPGKPGDVLTAEAQQVFEAGRTGVYDITVTNQQGELIAVMRGKSYRLKGRPVVEIQA
>Mature_155_residues
SEAAYRDVTAGLDPQALAEKVRDGMFERDQAARSLAMEITAVGPGRATIAMKVREDMLNGFRICHGGFITTLADTAFAYA
CNSGNEQTVASGISVDFMAPGKPGDVLTAEAQQVFEAGRTGVYDITVTNQQGELIAVMRGKSYRLKGRPVVEIQA

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=123, Percent_Identity=48.780487804878, Blast_Score=126, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 16679; Mature: 16548

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAAYRDVTAGLDPQALAEKVRDGMFERDQAARSLAMEITAVGPGRATIAMKVREDMLN
CCCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHEEEEEEECCCCEEEEHHHHHHHHC
GFRICHGGFITTLADTAFAYACNSGNEQTVASGISVDFMAPGKPGDVLTAEAQQVFEAGR
CEEEECCCHHHHHHHHHEEEEECCCCCCEECCCCEEEEECCCCCCCEEEHHHHHHHHCCC
TGVYDITVTNQQGELIAVMRGKSYRLKGRPVVEIQA
CCEEEEEEECCCCCEEEEEECCEEEECCCEEEEEEC
>Mature Secondary Structure 
SEAAYRDVTAGLDPQALAEKVRDGMFERDQAARSLAMEITAVGPGRATIAMKVREDMLN
CCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHEEEEEEECCCCEEEEHHHHHHHHC
GFRICHGGFITTLADTAFAYACNSGNEQTVASGISVDFMAPGKPGDVLTAEAQQVFEAGR
CEEEECCCHHHHHHHHHEEEEECCCCCCEECCCCEEEEECCCCCCCEEEHHHHHHHHCCC
TGVYDITVTNQQGELIAVMRGKSYRLKGRPVVEIQA
CCEEEEEEECCCCCEEEEEECCEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]