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The map label for this gene is vanB [H]

Identifier: 119896541

GI number: 119896541

Start: 277561

End: 278289

Strand: Reverse

Name: vanB [H]

Synonym: azo0250

Alternate gene names: 119896541

Gene position: 278289-277561 (Counterclockwise)

Preceding gene: 119896544

Following gene: 119896534

Centisome position: 6.36

GC content: 72.57

Gene sequence:

>729_bases
ATGTCCGCCTCCATGCCCAGCCGACCGCCGCCGTCCGACCGCATTCCGGTACGGGTGCGCAGCGTGCGGGACGAGGCCCG
CGACATCCGCAGCCTGGAGTTCGAAGCCGTGCGCGGCGGCGATCTGCCGCCGTGGCCGCCGGGTGCGCATGTTCTCGTCT
ATCCACCCGCGTGCCCGCCGCGCGCCTATGCCTTGTGCAGCCTGCCGGGCGAAGCTGCCACCTACCGCATCGCGGTGCGG
CGCGACGCCGGCACGGGTAACACGCCGGCCCAACTACATGCGCTGCACCCGGGCGACCAGCTGGCGGTATCGGCGCCGCA
CAGCAGCTTTGCGCTGGATGAGGGGGCGGAGCGTCACCTGCTCGTCGCCGGCGGCATCGGCATCGCGCCGCTGCTGTCGA
TGTTCCGCGCACTCGAACGGGGCGGCGCCGAAGCGGAACTGCACTATTTTGCGCGCAGCGAGGACGACGCGGCCTTTCTC
GCCGAACTCGAAGGCGCCCGCGCGCGCGGTACGCTGCACTGTCACTTCGGCCTCAGCGGCGGGGCAACGGCGGCGCGGCT
GCAGGATCTGTTCGCCACCGCGGCGCAGCGGCCGGGCGCAGGCGCCGGACTGGCGATCTACACCTGCGGCCCCGCCGGGC
TGATGCGGGCGGTGGATGTGGCCGCGGCGGCCAACCTGCATGCGGCGCAGTTGCGCCAGCAGCATTTCGGACCCACGCTG
GATCAGTAG

Upstream 100 bases:

>100_bases
GAACGCATCGCCGCCCCTTCGTGGATGGGTCCGCGATTTGCTTGGCATTGGCCCAGCCGCATCGTCACACCACCAACAAG
AACACCGAGGAGACTCCCCC

Downstream 100 bases:

>100_bases
GGACGCCGGACGGGCTCGCGCGGAACGGCGCGACTGAGGGCGGAAGACGGGAGCAGGGACAGGGCGCGGCGATCACGGGC
CGGGATGTTCAACACGATGC

Product: flavodoxin reductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSASMPSRPPPSDRIPVRVRSVRDEARDIRSLEFEAVRGGDLPPWPPGAHVLVYPPACPPRAYALCSLPGEAATYRIAVR
RDAGTGNTPAQLHALHPGDQLAVSAPHSSFALDEGAERHLLVAGGIGIAPLLSMFRALERGGAEAELHYFARSEDDAAFL
AELEGARARGTLHCHFGLSGGATAARLQDLFATAAQRPGAGAGLAIYTCGPAGLMRAVDVAAAANLHAAQLRQQHFGPTL
DQ

Sequences:

>Translated_242_residues
MSASMPSRPPPSDRIPVRVRSVRDEARDIRSLEFEAVRGGDLPPWPPGAHVLVYPPACPPRAYALCSLPGEAATYRIAVR
RDAGTGNTPAQLHALHPGDQLAVSAPHSSFALDEGAERHLLVAGGIGIAPLLSMFRALERGGAEAELHYFARSEDDAAFL
AELEGARARGTLHCHFGLSGGATAARLQDLFATAAQRPGAGAGLAIYTCGPAGLMRAVDVAAAANLHAAQLRQQHFGPTL
DQ
>Mature_241_residues
SASMPSRPPPSDRIPVRVRSVRDEARDIRSLEFEAVRGGDLPPWPPGAHVLVYPPACPPRAYALCSLPGEAATYRIAVRR
DAGTGNTPAQLHALHPGDQLAVSAPHSSFALDEGAERHLLVAGGIGIAPLLSMFRALERGGAEAELHYFARSEDDAAFLA
ELEGARARGTLHCHFGLSGGATAARLQDLFATAAQRPGAGAGLAIYTCGPAGLMRAVDVAAAANLHAAQLRQQHFGPTLD
Q

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=213, Percent_Identity=32.8638497652582, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 25386; Mature: 25255

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASMPSRPPPSDRIPVRVRSVRDEARDIRSLEFEAVRGGDLPPWPPGAHVLVYPPACPP
CCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCEEEEECCCCCC
RAYALCSLPGEAATYRIAVRRDAGTGNTPAQLHALHPGDQLAVSAPHSSFALDEGAERHL
CCEEEECCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCEE
LVAGGIGIAPLLSMFRALERGGAEAELHYFARSEDDAAFLAELEGARARGTLHCHFGLSG
EEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCC
GATAARLQDLFATAAQRPGAGAGLAIYTCGPAGLMRAVDVAAAANLHAAQLRQQHFGPTL
CCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
DQ
CC
>Mature Secondary Structure 
SASMPSRPPPSDRIPVRVRSVRDEARDIRSLEFEAVRGGDLPPWPPGAHVLVYPPACPP
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCEEEEECCCCCC
RAYALCSLPGEAATYRIAVRRDAGTGNTPAQLHALHPGDQLAVSAPHSSFALDEGAERHL
CCEEEECCCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCEE
LVAGGIGIAPLLSMFRALERGGAEAELHYFARSEDDAAFLAELEGARARGTLHCHFGLSG
EEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCC
GATAARLQDLFATAAQRPGAGAGLAIYTCGPAGLMRAVDVAAAANLHAAQLRQQHFGPTL
CCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
DQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098058 [H]