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The map label for this gene is mexA [H]

Identifier: 119896535

GI number: 119896535

Start: 270224

End: 271426

Strand: Direct

Name: mexA [H]

Synonym: azo0244

Alternate gene names: 119896535

Gene position: 270224-271426 (Clockwise)

Preceding gene: 119896533

Following gene: 119896536

Centisome position: 6.18

GC content: 71.74

Gene sequence:

>1203_bases
ATGCAGACCCCTCCCTCCCTGCTGTTAAAGCCACTCGCCGTCCTCGTCGCCGCCGCGCTGCTGCTCACCGCCTGCGGCGG
CCAGCAGGCACCGGCCGGTGCCCCGCCGGGCGGCGGCGCGCCCACCGTGGGCGTGGTGAAGGTGCAATCCGAGGCGGTGG
CGCTGACCACCGAGTTGCCGGGCCGCACCGCGCCTTACCAGATCGCCGAAGTGCGCCCGCAGGTCACCGGCATCGTCAAG
AAGCGCGCTTTCCGTGAAGGCAGCGAGGTCAAGGGCGGCGAGCTGCTGTACCAGATCGACCCCGCCACCTATCAGGCTGC
TTACGACAGCGCCAAGGCCAGCCTGGCGCGGGCCGAAGCCAATCTGTACACCGCCAAGCTGAAGGGCGAGCGCTATGCCG
ACCTGAGCCAGATCAACGCGATCAGCAAGCAGGCCAACGACGACGCGGTGGCCGCGCTGAAGCAGGCCGAGGCCGACGTG
GCGGCGGCGCGCGCGGCGGTGGACAAGGCCCGTATCGACCTCGAGTTCACGCGCGTCAGCGCCCCCATCCCGGGCCGCAT
CGGGCGTTCGTCGGTGACCGCCGGCGCCCTCGTCACCGCCAATCAGGCCGATCCGCTCGCCACCGTGCAGCAGCTCGATC
CGATCTATGTGGACGTCACCCAGTCCAGCGCCGAACTGCTCGCGCTGCGCCGCAGCCTGGCCGAAGGCAAGCTGCAGCGC
GCCGGCGACAACGCCGCCCGCGTGCAGCTGGTGATGGAAGACGGCAGCCTCTATCCGGCCGAAGGCAAGCTCGAGTTCTC
CGAAGTCTCGGTCGATACCGGCACCGGCAGCGTGGTCCTGCGCGCGGTGTTCCCCAACCCCAAGAACGACCTGCTGCCCG
GCATGTACGTGCGCGCCCGCTTCGCCCAGGGCGTGCGCCAGGACGCGGTGCTGGTGCCGCACGCCGGGGTGACCCGCGAC
GCGCGCGGCAACGCGGTGGTGATGGTGGTCAACGGCGACAACAAGGTCGAGGCCCGCCAGGTGCGCGCCGAGCAGGCGCT
GGCCGACAAGTGGGTGGTGAGCGAAGGCCTCGCCGCCGGCGACCGCGTGATCGTCGAAGGGCTGCAGAAGGTGCGGCCGG
GGGCGGACGTGCAGGTACAGGAGGTCGGTGCCGCCGCGCCGCAAGCCGCTGCGGCCGCGCCGACCGCCGCGGCCGCCAAG
TGA

Upstream 100 bases:

>100_bases
AGCTTCGCGGCGCGCCGGACGCAGAACAACCCGTCGTGTGCGGGTGTTTCCGTTGCCGCGGAAGCGCCTGCCCCGGCCAT
CCAACCGACAGAGGCTATCG

Downstream 100 bases:

>100_bases
GGAGCTGAGCCATGGCCCGTTTCTTCATCGACCGCCCCATCTTCGCCTGGGTGGTCGCGATCGTCATCATGCTCGCCGGC
GCACTCTCCATCCTGAGCCT

Product: membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MQTPPSLLLKPLAVLVAAALLLTACGGQQAPAGAPPGGGAPTVGVVKVQSEAVALTTELPGRTAPYQIAEVRPQVTGIVK
KRAFREGSEVKGGELLYQIDPATYQAAYDSAKASLARAEANLYTAKLKGERYADLSQINAISKQANDDAVAALKQAEADV
AAARAAVDKARIDLEFTRVSAPIPGRIGRSSVTAGALVTANQADPLATVQQLDPIYVDVTQSSAELLALRRSLAEGKLQR
AGDNAARVQLVMEDGSLYPAEGKLEFSEVSVDTGTGSVVLRAVFPNPKNDLLPGMYVRARFAQGVRQDAVLVPHAGVTRD
ARGNAVVMVVNGDNKVEARQVRAEQALADKWVVSEGLAAGDRVIVEGLQKVRPGADVQVQEVGAAAPQAAAAAPTAAAAK

Sequences:

>Translated_400_residues
MQTPPSLLLKPLAVLVAAALLLTACGGQQAPAGAPPGGGAPTVGVVKVQSEAVALTTELPGRTAPYQIAEVRPQVTGIVK
KRAFREGSEVKGGELLYQIDPATYQAAYDSAKASLARAEANLYTAKLKGERYADLSQINAISKQANDDAVAALKQAEADV
AAARAAVDKARIDLEFTRVSAPIPGRIGRSSVTAGALVTANQADPLATVQQLDPIYVDVTQSSAELLALRRSLAEGKLQR
AGDNAARVQLVMEDGSLYPAEGKLEFSEVSVDTGTGSVVLRAVFPNPKNDLLPGMYVRARFAQGVRQDAVLVPHAGVTRD
ARGNAVVMVVNGDNKVEARQVRAEQALADKWVVSEGLAAGDRVIVEGLQKVRPGADVQVQEVGAAAPQAAAAAPTAAAAK
>Mature_400_residues
MQTPPSLLLKPLAVLVAAALLLTACGGQQAPAGAPPGGGAPTVGVVKVQSEAVALTTELPGRTAPYQIAEVRPQVTGIVK
KRAFREGSEVKGGELLYQIDPATYQAAYDSAKASLARAEANLYTAKLKGERYADLSQINAISKQANDDAVAALKQAEADV
AAARAAVDKARIDLEFTRVSAPIPGRIGRSSVTAGALVTANQADPLATVQQLDPIYVDVTQSSAELLALRRSLAEGKLQR
AGDNAARVQLVMEDGSLYPAEGKLEFSEVSVDTGTGSVVLRAVFPNPKNDLLPGMYVRARFAQGVRQDAVLVPHAGVTRD
ARGNAVVMVVNGDNKVEARQVRAEQALADKWVVSEGLAAGDRVIVEGLQKVRPGADVQVQEVGAAAPQAAAAAPTAAAAK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=386, Percent_Identity=55.699481865285, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=54.2553191489362, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1789929, Length=365, Percent_Identity=49.041095890411, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI87082036, Length=364, Percent_Identity=29.9450549450549, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41473; Mature: 41473

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPPSLLLKPLAVLVAAALLLTACGGQQAPAGAPPGGGAPTVGVVKVQSEAVALTTELP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCC
GRTAPYQIAEVRPQVTGIVKKRAFREGSEVKGGELLYQIDPATYQAAYDSAKASLARAEA
CCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
NLYTAKLKGERYADLSQINAISKQANDDAVAALKQAEADVAAARAAVDKARIDLEFTRVS
HEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEC
APIPGRIGRSSVTAGALVTANQADPLATVQQLDPIYVDVTQSSAELLALRRSLAEGKLQR
CCCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHH
AGDNAARVQLVMEDGSLYPAEGKLEFSEVSVDTGTGSVVLRAVFPNPKNDLLPGMYVRAR
CCCCCEEEEEEEECCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHH
FAQGVRQDAVLVPHAGVTRDARGNAVVMVVNGDNKVEARQVRAEQALADKWVVSEGLAAG
HHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DRVIVEGLQKVRPGADVQVQEVGAAAPQAAAAAPTAAAAK
CHHHHHHHHHHCCCCCEEHHHHCCCCCCHHHCCCCHHCCC
>Mature Secondary Structure
MQTPPSLLLKPLAVLVAAALLLTACGGQQAPAGAPPGGGAPTVGVVKVQSEAVALTTELP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCC
GRTAPYQIAEVRPQVTGIVKKRAFREGSEVKGGELLYQIDPATYQAAYDSAKASLARAEA
CCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
NLYTAKLKGERYADLSQINAISKQANDDAVAALKQAEADVAAARAAVDKARIDLEFTRVS
HEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEC
APIPGRIGRSSVTAGALVTANQADPLATVQQLDPIYVDVTQSSAELLALRRSLAEGKLQR
CCCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHH
AGDNAARVQLVMEDGSLYPAEGKLEFSEVSVDTGTGSVVLRAVFPNPKNDLLPGMYVRAR
CCCCCEEEEEEEECCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHH
FAQGVRQDAVLVPHAGVTRDARGNAVVMVVNGDNKVEARQVRAEQALADKWVVSEGLAAG
HHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DRVIVEGLQKVRPGADVQVQEVGAAAPQAAAAAPTAAAAK
CHHHHHHHHHHCCCCCEEHHHHCCCCCCHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]