Click here to switch to the map view.

The map label for this gene is yueD [H]

Identifier: 119896480

GI number: 119896480

Start: 210014

End: 210739

Strand: Reverse

Name: yueD [H]

Synonym: azo0189

Alternate gene names: 119896480

Gene position: 210739-210014 (Counterclockwise)

Preceding gene: 119896484

Following gene: 119896479

Centisome position: 4.82

GC content: 61.71

Gene sequence:

>726_bases
GTGAAAGCAATCGTCACAGGACACAGCCGAGGACTCGGCGAAGCGCTCGCCATCGGCCTGCTCGAGCGCGGTTTGGAGGT
GCTCGGTGTCTCCCGCCACAGCCATGCGGAAATGGCCGCCCGCTTTCCGCGATTCTCCGAATCCAGGGTCGATCTGGCAG
ACGTAGCGGCGGTCTGCCGTTTCATCGAGAGCCCCGTATTCCAAGCGTTCGGCAATGCTCGCGAACCGGTCATCCTGATC
AACAACGCCGCAATGCTACAGCCGGTCGGGAGAAGCGGAAGTTTGGCCGCGGCTGAAATTCAGCAGGCGGTGAACGTCAA
TGTCCTCGGGCCCTTGCTGCTGACAAACGCCGTCGTTCAATGCGCGGACCCCGATCTATCCCGCCGGATTCTCCACATTT
CCAGTGGCGCTGCGCGGACGGCCTATTCAGGTTGGAGCATCTACGGAGCAACCAAAGCAGCCCTGGACCATCATGCCCGT
TGCGTCAGCGAGGAGGGGCGCGCCCATGTCCGCATTTGCAGCGTCGCTCCCGGGGTGTTGGATACCAGGATGCAAGCCGA
CGTCCGCCAACTCGAAACCGGTCAGTTTCCCGCGCGTGAGAGGTTCATTTCGCTGAAGGACAGCGGGCAACTTGTTCCGC
CGGCAGAGGCGGCGGCCAAATTGTTGGCGTTTCTATTCGCGCCTGCATTCGGAGACACGCCGACAGCTGACGTTCGGACT
TTCTAG

Upstream 100 bases:

>100_bases
ATGTTCCAATCCCTGATAGGCGGGAAAACCCGGGCGGTTCTGATTGCATTTCCGGTCCGTTTCAGCCCCCACGCCTATTC
TCACTGCGCTGGAGAAACTC

Downstream 100 bases:

>100_bases
TTCCAATAAAAAAGCCCGCACGATAGATCGTGCGGGCTTTTTTTGAGTAGTTAGTCTGACGATGACCTACTTTCACGAGC
GCGGGCTCACTATCATCGGC

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKAIVTGHSRGLGEALAIGLLERGLEVLGVSRHSHAEMAARFPRFSESRVDLADVAAVCRFIESPVFQAFGNAREPVILI
NNAAMLQPVGRSGSLAAAEIQQAVNVNVLGPLLLTNAVVQCADPDLSRRILHISSGAARTAYSGWSIYGATKAALDHHAR
CVSEEGRAHVRICSVAPGVLDTRMQADVRQLETGQFPARERFISLKDSGQLVPPAEAAAKLLAFLFAPAFGDTPTADVRT
F

Sequences:

>Translated_241_residues
MKAIVTGHSRGLGEALAIGLLERGLEVLGVSRHSHAEMAARFPRFSESRVDLADVAAVCRFIESPVFQAFGNAREPVILI
NNAAMLQPVGRSGSLAAAEIQQAVNVNVLGPLLLTNAVVQCADPDLSRRILHISSGAARTAYSGWSIYGATKAALDHHAR
CVSEEGRAHVRICSVAPGVLDTRMQADVRQLETGQFPARERFISLKDSGQLVPPAEAAAKLLAFLFAPAFGDTPTADVRT
F
>Mature_241_residues
MKAIVTGHSRGLGEALAIGLLERGLEVLGVSRHSHAEMAARFPRFSESRVDLADVAAVCRFIESPVFQAFGNAREPVILI
NNAAMLQPVGRSGSLAAAEIQQAVNVNVLGPLLLTNAVVQCADPDLSRRILHISSGAARTAYSGWSIYGATKAALDHHAR
CVSEEGRAHVRICSVAPGVLDTRMQADVRQLETGQFPARERFISLKDSGQLVPPAEAAAKLLAFLFAPAFGDTPTADVRT
F

Specific function: Reduces benzil stereospecifically to (S)-benzoin. Can also reduces 1-phenyl-1,2-propanedione, 1,4-naphthoquinone, 1-(4- methyl-phenyl)-2-phenyl-ethane-1,2-dione, 1-(4-fluoro-phenyl)-2- phenyl-ethane-1,2-dione, methyl benzoylformate, p- nitrobenzaldehyde i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4507185, Length=253, Percent_Identity=32.4110671936759, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786812, Length=182, Percent_Identity=32.967032967033, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322227, Length=236, Percent_Identity=33.4745762711864, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322226, Length=232, Percent_Identity=28.0172413793103, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 25677; Mature: 25677

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIVTGHSRGLGEALAIGLLERGLEVLGVSRHSHAEMAARFPRFSESRVDLADVAAVCR
CCEEECCCCCCHHHHHHHHHHHHCHHHEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHH
FIESPVFQAFGNAREPVILINNAAMLQPVGRSGSLAAAEIQQAVNVNVLGPLLLTNAVVQ
HHHHHHHHHHCCCCCCEEEEECCCEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
CADPDLSRRILHISSGAARTAYSGWSIYGATKAALDHHARCVSEEGRAHVRICSVAPGVL
CCCCCHHHEEEEECCCCCHHCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
DTRMQADVRQLETGQFPARERFISLKDSGQLVPPAEAAAKLLAFLFAPAFGDTPTADVRT
HHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
F
C
>Mature Secondary Structure
MKAIVTGHSRGLGEALAIGLLERGLEVLGVSRHSHAEMAARFPRFSESRVDLADVAAVCR
CCEEECCCCCCHHHHHHHHHHHHCHHHEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHH
FIESPVFQAFGNAREPVILINNAAMLQPVGRSGSLAAAEIQQAVNVNVLGPLLLTNAVVQ
HHHHHHHHHHCCCCCCEEEEECCCEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
CADPDLSRRILHISSGAARTAYSGWSIYGATKAALDHHARCVSEEGRAHVRICSVAPGVL
CCCCCHHHEEEEECCCCCHHCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
DTRMQADVRQLETGQFPARERFISLKDSGQLVPPAEAAAKLLAFLFAPAFGDTPTADVRT
HHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11745140; 11796169 [H]