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The map label for this gene is pbpA [H]

Identifier: 119896469

GI number: 119896469

Start: 192797

End: 194686

Strand: Direct

Name: pbpA [H]

Synonym: azo0177

Alternate gene names: 119896469

Gene position: 192797-194686 (Clockwise)

Preceding gene: 119896468

Following gene: 119896470

Centisome position: 4.41

GC content: 68.2

Gene sequence:

>1890_bases
ATGACCGAGTTTCGCACCCCGGCCCAGGAAAAATCGCGCTTCCGCAACCGTGTGGTGGTGGCAGCGCTGTTCATCCTCGC
CTGCTTCGCGCTGCTGGCGATGCGCTTCTACTACCTGCAGGTGACGCGGCACGCGTATTTCCACACCCGCGCCGAGGACA
ACCGCATCGCACTGCTGCCGGTCGTGCCCAACCGCGGCACCATCACCGACCGCAACGGCGTCGTGCTGGCGCGCAACTAT
GCCGCCTACACGCTCGAGATCACGCCATCGAAAGCGGGCGACCTGGAGGCGACGATCGACGGCCTCGCCGAAGTCGTCAC
CATCGAGCCGCGCGACCGCCGCCGGTTCCGCAAGGTGCTGGAGGAAAGCCGCAACTTCGAGAGCGTGCCGATCCGCACCC
GGCTGACCGACGAGGAGGTGGCGCGCTTTACCGCGCAGCGTTACCGCTTCCCCGGCGTGGAGGTCCAGGCGCGGCTGTTC
CGCGACTATCCCGAGGGCGAGGTCGCGTCGCACGTGATCGGCTACATCGGCCGCATCAACGATCGCGACGTGGAGCGCAT
CGAGGAGCGCGGCCAGACCGCCAACTACCGCGGCTCCGAGTACATCGGCAAGGCCGGGCTGGAGCAGTCGTACGAGGCCG
AACTCCACGGCCAGACCGGTTTCGAGCAGGTGGAAGTGAACGCCGGCGGGCGCGCCGTGCGGCGGCTGTCGCGCACCGCG
GCGATGGTCGGCAACAACCTCGAGCTGACGCTGGACATCGAACTGCAGAAGATCGCCGAAAACGCGTTCGGCAACCGGCG
CGGCGCGCTGGTCGCGATCGAGCCCGGCACCGGTGGCGTGCTCGCGCTGGTGTCCAAACCCACCTTCGATCCCAACCTGT
TCGTCGAGGGCATCTCCGCGCAGGACTGGAAGCAGCTCAACGATTCGCCCGACCACCCGCTGCTCAACCGCGCCATCTTC
AGCGCCTACCCGCCCGGCTCCACCTTCAAGCCCTTCATGGCGCTCGGTGCGCTGACCACCGGCAAGCGCACGCCGGGCTA
CGTGATGGCCGATCCGGGCTACTTCGTGTCGGGCGGCCACCGCTTCATGGACGACAAGGTCGGCGGCCACGGCATGGTGG
ATCTGCACAAGTCCATCGTCGTGTCCTGCAACACCTTCTACTACCAGCTGGCCAACGACATGGGCATCGACGGCATCGCC
GACTTCATGCGGCCGCTCGGCTTCGGCTCGCGCACCGGCATCGACGTGCCGGGCGAGGCGGAAGGCGTGCTGCCGTCGCC
GGCCTGGAAGAAGAAGCGCTTCCGTCGTCCCGAGCAGCAGCGCTGGTTCGGCGGCGAGACGATTTCGGTCGGCATCGGCC
AAGGCTACAACGCCTACACCCCGCTCCAGCTTGCCAACGCACTGGCCACCGTCGTGAGCGACGGCAAGGTGTTCCGCCCC
CATATTGTCCGGCACGTGGTCGACGCGCGCGACAACCGCCGCGTGATCGAGCCGGAGCCGCTGCGCCAGCTCAACTTCAA
GCCGGAGCACCTCGCGCTCGTGCGGCGCGCGATGGTGGACGTCAACAAGAGCGGCACCGGTGCACGCGCGTTCGCCGGCG
CGCCCTACGAGGTCGGCGGCAAGACCGGCACCGCGCAGGTGTTCTCGTTGAAGGGCGCCAAGTACGTCGAGGGGCGCGTC
AGCGAGCGCCTGCGCGACCACTCCTGGTTCATCGCTTACGCGCCGGCCGACAACCCCAAGATCGCGCTCGCGGTACTGGT
CGAAAACGGCGGCTTCGGCGCCCAGTCGGCGGCCCCGATCGCACGCCAGGTGATCGACTACCACCTGCTCGGCAAGCGGC
CCAACCAGCCCGCCGGCGAGGATGCGGATGCACCGGAGAGCACCGAATGA

Upstream 100 bases:

>100_bases
CACCGCCGTCGCGCTGTGGGTGCCGCTGAACTACCTGCTGCTGCTGCCGCAATACCAGCCGGTCGACCGGGACGACAACC
GGCCGATCTGATCCGCGCCC

Downstream 100 bases:

>100_bases
GCGATCACCGCTTCAATCCGCTGCTGTTGCTGCGCGCGGTGCTGCGTCCGATCGACCCGGTGCTGATGCTGGTGCTGATG
ATGTTGCTCGGCTACGCCTT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MTEFRTPAQEKSRFRNRVVVAALFILACFALLAMRFYYLQVTRHAYFHTRAEDNRIALLPVVPNRGTITDRNGVVLARNY
AAYTLEITPSKAGDLEATIDGLAEVVTIEPRDRRRFRKVLEESRNFESVPIRTRLTDEEVARFTAQRYRFPGVEVQARLF
RDYPEGEVASHVIGYIGRINDRDVERIEERGQTANYRGSEYIGKAGLEQSYEAELHGQTGFEQVEVNAGGRAVRRLSRTA
AMVGNNLELTLDIELQKIAENAFGNRRGALVAIEPGTGGVLALVSKPTFDPNLFVEGISAQDWKQLNDSPDHPLLNRAIF
SAYPPGSTFKPFMALGALTTGKRTPGYVMADPGYFVSGGHRFMDDKVGGHGMVDLHKSIVVSCNTFYYQLANDMGIDGIA
DFMRPLGFGSRTGIDVPGEAEGVLPSPAWKKKRFRRPEQQRWFGGETISVGIGQGYNAYTPLQLANALATVVSDGKVFRP
HIVRHVVDARDNRRVIEPEPLRQLNFKPEHLALVRRAMVDVNKSGTGARAFAGAPYEVGGKTGTAQVFSLKGAKYVEGRV
SERLRDHSWFIAYAPADNPKIALAVLVENGGFGAQSAAPIARQVIDYHLLGKRPNQPAGEDADAPESTE

Sequences:

>Translated_629_residues
MTEFRTPAQEKSRFRNRVVVAALFILACFALLAMRFYYLQVTRHAYFHTRAEDNRIALLPVVPNRGTITDRNGVVLARNY
AAYTLEITPSKAGDLEATIDGLAEVVTIEPRDRRRFRKVLEESRNFESVPIRTRLTDEEVARFTAQRYRFPGVEVQARLF
RDYPEGEVASHVIGYIGRINDRDVERIEERGQTANYRGSEYIGKAGLEQSYEAELHGQTGFEQVEVNAGGRAVRRLSRTA
AMVGNNLELTLDIELQKIAENAFGNRRGALVAIEPGTGGVLALVSKPTFDPNLFVEGISAQDWKQLNDSPDHPLLNRAIF
SAYPPGSTFKPFMALGALTTGKRTPGYVMADPGYFVSGGHRFMDDKVGGHGMVDLHKSIVVSCNTFYYQLANDMGIDGIA
DFMRPLGFGSRTGIDVPGEAEGVLPSPAWKKKRFRRPEQQRWFGGETISVGIGQGYNAYTPLQLANALATVVSDGKVFRP
HIVRHVVDARDNRRVIEPEPLRQLNFKPEHLALVRRAMVDVNKSGTGARAFAGAPYEVGGKTGTAQVFSLKGAKYVEGRV
SERLRDHSWFIAYAPADNPKIALAVLVENGGFGAQSAAPIARQVIDYHLLGKRPNQPAGEDADAPESTE
>Mature_628_residues
TEFRTPAQEKSRFRNRVVVAALFILACFALLAMRFYYLQVTRHAYFHTRAEDNRIALLPVVPNRGTITDRNGVVLARNYA
AYTLEITPSKAGDLEATIDGLAEVVTIEPRDRRRFRKVLEESRNFESVPIRTRLTDEEVARFTAQRYRFPGVEVQARLFR
DYPEGEVASHVIGYIGRINDRDVERIEERGQTANYRGSEYIGKAGLEQSYEAELHGQTGFEQVEVNAGGRAVRRLSRTAA
MVGNNLELTLDIELQKIAENAFGNRRGALVAIEPGTGGVLALVSKPTFDPNLFVEGISAQDWKQLNDSPDHPLLNRAIFS
AYPPGSTFKPFMALGALTTGKRTPGYVMADPGYFVSGGHRFMDDKVGGHGMVDLHKSIVVSCNTFYYQLANDMGIDGIAD
FMRPLGFGSRTGIDVPGEAEGVLPSPAWKKKRFRRPEQQRWFGGETISVGIGQGYNAYTPLQLANALATVVSDGKVFRPH
IVRHVVDARDNRRVIEPEPLRQLNFKPEHLALVRRAMVDVNKSGTGARAFAGAPYEVGGKTGTAQVFSLKGAKYVEGRVS
ERLRDHSWFIAYAPADNPKIALAVLVENGGFGAQSAAPIARQVIDYHLLGKRPNQPAGEDADAPESTE

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=615, Percent_Identity=42.9268292682927, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1786272, Length=596, Percent_Identity=26.1744966442953, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69584; Mature: 69453

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00496 PII_GLNB_UMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFRTPAQEKSRFRNRVVVAALFILACFALLAMRFYYLQVTRHAYFHTRAEDNRIALLP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEE
VVPNRGTITDRNGVVLARNYAAYTLEITPSKAGDLEATIDGLAEVVTIEPRDRRRFRKVL
ECCCCCCEECCCCEEEEECCEEEEEEECCCCCCCCHHHHHCCEEEEEECCHHHHHHHHHH
EESRNFESVPIRTRLTDEEVARFTAQRYRFPGVEVQARLFRDYPEGEVASHVIGYIGRIN
HHCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC
DRDVERIEERGQTANYRGSEYIGKAGLEQSYEAELHGQTGFEQVEVNAGGRAVRRLSRTA
CHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHH
AMVGNNLELTLDIELQKIAENAFGNRRGALVAIEPGTGGVLALVSKPTFDPNLFVEGISA
HHHCCCEEEEEEEHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEECCCH
QDWKQLNDSPDHPLLNRAIFSAYPPGSTFKPFMALGALTTGKRTPGYVMADPGYFVSGGH
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCEEECCCC
RFMDDKVGGHGMVDLHKSIVVSCNTFYYQLANDMGIDGIADFMRPLGFGSRTGIDVPGEA
EECCCCCCCCCHHEECCEEEEEHHHHEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
EGVLPSPAWKKKRFRRPEQQRWFGGETISVGIGQGYNAYTPLQLANALATVVSDGKVFRP
CCCCCCCHHHHHHHCCCHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEHH
HIVRHVVDARDNRRVIEPEPLRQLNFKPEHLALVRRAMVDVNKSGTGARAFAGAPYEVGG
HHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
KTGTAQVFSLKGAKYVEGRVSERLRDHSWFIAYAPADNPKIALAVLVENGGFGAQSAAPI
CCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCHHH
ARQVIDYHLLGKRPNQPAGEDADAPESTE
HHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEFRTPAQEKSRFRNRVVVAALFILACFALLAMRFYYLQVTRHAYFHTRAEDNRIALLP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEE
VVPNRGTITDRNGVVLARNYAAYTLEITPSKAGDLEATIDGLAEVVTIEPRDRRRFRKVL
ECCCCCCEECCCCEEEEECCEEEEEEECCCCCCCCHHHHHCCEEEEEECCHHHHHHHHHH
EESRNFESVPIRTRLTDEEVARFTAQRYRFPGVEVQARLFRDYPEGEVASHVIGYIGRIN
HHCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC
DRDVERIEERGQTANYRGSEYIGKAGLEQSYEAELHGQTGFEQVEVNAGGRAVRRLSRTA
CHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHH
AMVGNNLELTLDIELQKIAENAFGNRRGALVAIEPGTGGVLALVSKPTFDPNLFVEGISA
HHHCCCEEEEEEEHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEECCCH
QDWKQLNDSPDHPLLNRAIFSAYPPGSTFKPFMALGALTTGKRTPGYVMADPGYFVSGGH
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCEEECCCC
RFMDDKVGGHGMVDLHKSIVVSCNTFYYQLANDMGIDGIADFMRPLGFGSRTGIDVPGEA
EECCCCCCCCCHHEECCEEEEEHHHHEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
EGVLPSPAWKKKRFRRPEQQRWFGGETISVGIGQGYNAYTPLQLANALATVVSDGKVFRP
CCCCCCCHHHHHHHCCCHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEHH
HIVRHVVDARDNRRVIEPEPLRQLNFKPEHLALVRRAMVDVNKSGTGARAFAGAPYEVGG
HHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
KTGTAQVFSLKGAKYVEGRVSERLRDHSWFIAYAPADNPKIALAVLVENGGFGAQSAAPI
CCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCHHH
ARQVIDYHLLGKRPNQPAGEDADAPESTE
HHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]