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The map label for this gene is sigW [H]

Identifier: 119871513

GI number: 119871513

Start: 5732942

End: 5733619

Strand: Direct

Name: sigW [H]

Synonym: Mkms_5490

Alternate gene names: 119871513

Gene position: 5732942-5733619 (Clockwise)

Preceding gene: 119871512

Following gene: 119871514

Centisome position: 99.93

GC content: 72.42

Gene sequence:

>678_bases
GTGGTGAGCTTCGACGCCGTCGGCCGGTCCGACGCCCAGCTGCTGGCCGATCATGTCGCGGGTGACCGCTACGCGTTCGC
GGAGTTGTTCGTCCGCCACCAGCGCCAGCTCGAGCGGCTCGCGCGGATCACCAGCAGCAACCGCGAGGACGCCGCCGACG
CGCTGCAGGAGGCGATGCTCTCGGCGCACCGCAAGGCCGCGAGCTTCCGCTTCGACTCCGCGGTGAGCAGCTGGCTGTAC
CGCATCGTCGTCAACGCGTGCCTGGACCGGCTGCGCACCAACCGCACACACGCCACCAGCACGCTCGTCGACGACGTCGG
ATCGGCCGGCGACCCGACGTCCAGGGTGGACACCGCGATCGTCATCGAACGGGCCCTGCTGCGGTTGCCGGTCGAACAAC
GCGCCGCCATCGTCGCCGTCGACATGCAGGGGTACTCGGTCGCCGAAACCGCCCGGATGCTGGGCGTCGCGGAAGGCACG
GTCAAGAGTCGGTGCAGCCGGGGCCGGGCGAAACTGGCCGAGACATTGCGCGGTCTCGACGAGACGGAGACGGCATCGCC
GCCGCCCGCCGGCCGCGGCTGCCACCCGGTGCCGCGGTCGGCCCGTAGCCGCCGCCGTCCCACTATCGAGCGCGCCGTGC
GTCACGCGGGTGGCGGGACACCGGTCGACCGGCCGTAG

Upstream 100 bases:

>100_bases
AGGCGGTTATCCACAGGCTCGGTGAGCCGCGACCTCTGTCGACGAGCCGTCGGCGAACTACGTGCGCGAGAGCACCCCCG
GACCCGCTTAGGGTTCGGCT

Downstream 100 bases:

>100_bases
CGTGACCCTCCAGGCAGTCGGGAACACCCGGACCTAGGCTGGTGTTGTACCCGTGCCGGTGCGAAACGGCAGAAAGGCTC
ACATGACCAGCTCATCGACG

Product: RNA polymerase sigma factor SigM

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MVSFDAVGRSDAQLLADHVAGDRYAFAELFVRHQRQLERLARITSSNREDAADALQEAMLSAHRKAASFRFDSAVSSWLY
RIVVNACLDRLRTNRTHATSTLVDDVGSAGDPTSRVDTAIVIERALLRLPVEQRAAIVAVDMQGYSVAETARMLGVAEGT
VKSRCSRGRAKLAETLRGLDETETASPPPAGRGCHPVPRSARSRRRPTIERAVRHAGGGTPVDRP

Sequences:

>Translated_225_residues
MVSFDAVGRSDAQLLADHVAGDRYAFAELFVRHQRQLERLARITSSNREDAADALQEAMLSAHRKAASFRFDSAVSSWLY
RIVVNACLDRLRTNRTHATSTLVDDVGSAGDPTSRVDTAIVIERALLRLPVEQRAAIVAVDMQGYSVAETARMLGVAEGT
VKSRCSRGRAKLAETLRGLDETETASPPPAGRGCHPVPRSARSRRRPTIERAVRHAGGGTPVDRP
>Mature_225_residues
MVSFDAVGRSDAQLLADHVAGDRYAFAELFVRHQRQLERLARITSSNREDAADALQEAMLSAHRKAASFRFDSAVSSWLY
RIVVNACLDRLRTNRTHATSTLVDDVGSAGDPTSRVDTAIVIERALLRLPVEQRAAIVAVDMQGYSVAETARMLGVAEGT
VKSRCSRGRAKLAETLRGLDETETASPPPAGRGCHPVPRSARSRRRPTIERAVRHAGGGTPVDRP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=183, Percent_Identity=32.2404371584699, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 24497; Mature: 24497

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSFDAVGRSDAQLLADHVAGDRYAFAELFVRHQRQLERLARITSSNREDAADALQEAML
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SAHRKAASFRFDSAVSSWLYRIVVNACLDRLRTNRTHATSTLVDDVGSAGDPTSRVDTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VIERALLRLPVEQRAAIVAVDMQGYSVAETARMLGVAEGTVKSRCSRGRAKLAETLRGLD
HHHHHHHHCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETETASPPPAGRGCHPVPRSARSRRRPTIERAVRHAGGGTPVDRP
CCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVSFDAVGRSDAQLLADHVAGDRYAFAELFVRHQRQLERLARITSSNREDAADALQEAML
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SAHRKAASFRFDSAVSSWLYRIVVNACLDRLRTNRTHATSTLVDDVGSAGDPTSRVDTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VIERALLRLPVEQRAAIVAVDMQGYSVAETARMLGVAEGTVKSRCSRGRAKLAETLRGLD
HHHHHHHHCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETETASPPPAGRGCHPVPRSARSRRRPTIERAVRHAGGGTPVDRP
CCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]