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The map label for this gene is dosC [H]

Identifier: 119871383

GI number: 119871383

Start: 5595072

End: 5596154

Strand: Direct

Name: dosC [H]

Synonym: Mkms_5359

Alternate gene names: 119871383

Gene position: 5595072-5596154 (Clockwise)

Preceding gene: 119871380

Following gene: 119871388

Centisome position: 97.52

GC content: 70.18

Gene sequence:

>1083_bases
ATGCAATGGGTGGGTAGATGGTGGCGACAGCCCGACCATTTCGATTGGCTCAGCGGTTATCTGCAGACCCGCGGAATGGC
CACCGCGATGCGCCGAGGGCTGGCGCTGGTCGCCGCATCCTTGGCGTTGGTGCCGGTCAACGCGCTGTGGGGGCCTGCCT
CCATCGACGACCGGTTGGCGGTGAGTCTCGCCCTGGTGGCTTCGCTGGCGGGGCTGAGCCTGGCGGTGCTGTGGCTCAAG
CGCTGGCCCACCAGAACGGAGTCGGTGCTGTTCGCCGCAATCGGCAGCGCAACCATCGCCGGCGGCTGCCTCTGGCAGGC
GGAGCCCCTGATCGGCCTGACGTTCTGCACCGCGCTCGCGGTGTCCGGCGGGTACATCGCGTTCTTCCACACGGCCAGGT
ACATGCTGATGAACTTCGTGCTGGCGGTGGGGGTGGGCGCCTGGCAGGCGATCCGGGTGGCCGCCGAGGGTGAACCCGTG
CTGGCCCTCACCGGCTACTTCCTGGTGCTCGAACTGAACATCGGTGTGCCGTTCGCCATCCAGGTCGTGGTGCGCTCGCT
CGGTACCGACCTGCTGCAATCCGACCGCGATCCGCTCACCGGACTCCTCAACCGGCGGGCGTTCACCCACGCCGTGGTCG
GACGGCTGGTGGCTCGCGCCGACCGGGCCCACCTGGCGGTGGCGTTGATCGACCTCGACCGGTTCAAGGCGATCAACGAT
CGGCACGGCCACGCCAGCGGGGACGCCGCCCTGGTCGACGTCGCCGGGGCGTTGACGGCCGCGGTCACCGAGACCGCGCT
GGTGTGCCGTATGGGTGGCGAGGAGTTCCTGATCGCCGACATCGTGGCGTCGGCCGCGCCGACGGAGTGGGCCCAGGGGC
TGTGCGCGGCGGTGACGTCGACACCGTTCCGGCTCACGGCCAGCGTCGGCATCGCCACGGTCCCGCTGCGCGACGTCAAA
CCGGAGCAGGCCGACGAGACGTTCCGCCGATTGGTCACCGAGGCCGACGCCGCCATGTACGCCGCCAAGCGGCGCGGCGG
CAACCAGATCCAGCACGCCGGGGACGCCCGAACCCCTAGGTGA

Upstream 100 bases:

>100_bases
CGGCCGTACCGGGAAATCCTGACGTTCACAAGTACCGCCGACGGGCCTCCCTGTACCGTGAGCAAACACGCGCCGAGGCG
TGTACACGGGGGAGCCGTCG

Downstream 100 bases:

>100_bases
GATTCGCCGCCCATCGCAGACCCTCCGCCGAGTACGTCGCGATCCAGTCGGCGACCGACCGCGGGTCGTACCGGGCGATG
AAGGCTTCCTCCTGGGCGGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MQWVGRWWRQPDHFDWLSGYLQTRGMATAMRRGLALVAASLALVPVNALWGPASIDDRLAVSLALVASLAGLSLAVLWLK
RWPTRTESVLFAAIGSATIAGGCLWQAEPLIGLTFCTALAVSGGYIAFFHTARYMLMNFVLAVGVGAWQAIRVAAEGEPV
LALTGYFLVLELNIGVPFAIQVVVRSLGTDLLQSDRDPLTGLLNRRAFTHAVVGRLVARADRAHLAVALIDLDRFKAIND
RHGHASGDAALVDVAGALTAAVTETALVCRMGGEEFLIADIVASAAPTEWAQGLCAAVTSTPFRLTASVGIATVPLRDVK
PEQADETFRRLVTEADAAMYAAKRRGGNQIQHAGDARTPR

Sequences:

>Translated_360_residues
MQWVGRWWRQPDHFDWLSGYLQTRGMATAMRRGLALVAASLALVPVNALWGPASIDDRLAVSLALVASLAGLSLAVLWLK
RWPTRTESVLFAAIGSATIAGGCLWQAEPLIGLTFCTALAVSGGYIAFFHTARYMLMNFVLAVGVGAWQAIRVAAEGEPV
LALTGYFLVLELNIGVPFAIQVVVRSLGTDLLQSDRDPLTGLLNRRAFTHAVVGRLVARADRAHLAVALIDLDRFKAIND
RHGHASGDAALVDVAGALTAAVTETALVCRMGGEEFLIADIVASAAPTEWAQGLCAAVTSTPFRLTASVGIATVPLRDVK
PEQADETFRRLVTEADAAMYAAKRRGGNQIQHAGDARTPR
>Mature_360_residues
MQWVGRWWRQPDHFDWLSGYLQTRGMATAMRRGLALVAASLALVPVNALWGPASIDDRLAVSLALVASLAGLSLAVLWLK
RWPTRTESVLFAAIGSATIAGGCLWQAEPLIGLTFCTALAVSGGYIAFFHTARYMLMNFVLAVGVGAWQAIRVAAEGEPV
LALTGYFLVLELNIGVPFAIQVVVRSLGTDLLQSDRDPLTGLLNRRAFTHAVVGRLVARADRAHLAVALIDLDRFKAIND
RHGHASGDAALVDVAGALTAAVTETALVCRMGGEEFLIADIVASAAPTEWAQGLCAAVTSTPFRLTASVGIATVPLRDVK
PEQADETFRRLVTEADAAMYAAKRRGGNQIQHAGDARTPR

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=166, Percent_Identity=36.7469879518072, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=36.5269461077844, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=186, Percent_Identity=32.258064516129, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787541, Length=181, Percent_Identity=30.939226519337, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=30, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788381, Length=188, Percent_Identity=34.0425531914894, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=162, Percent_Identity=33.9506172839506, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=28.4023668639053, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787816, Length=233, Percent_Identity=27.8969957081545, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=32.3353293413174, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38462; Mature: 38462

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWVGRWWRQPDHFDWLSGYLQTRGMATAMRRGLALVAASLALVPVNALWGPASIDDRLA
CCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VSLALVASLAGLSLAVLWLKRWPTRTESVLFAAIGSATIAGGCLWQAEPLIGLTFCTALA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCEEECCCCHHHHHHHHHHH
VSGGYIAFFHTARYMLMNFVLAVGVGAWQAIRVAAEGEPVLALTGYFLVLELNIGVPFAI
HCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCEEEEEEEEEEEEECCCCCHHH
QVVVRSLGTDLLQSDRDPLTGLLNRRAFTHAVVGRLVARADRAHLAVALIDLDRFKAIND
HHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEHHHHHHCCC
RHGHASGDAALVDVAGALTAAVTETALVCRMGGEEFLIADIVASAAPTEWAQGLCAAVTS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCC
TPFRLTASVGIATVPLRDVKPEQADETFRRLVTEADAAMYAAKRRGGNQIQHAGDARTPR
CCEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQWVGRWWRQPDHFDWLSGYLQTRGMATAMRRGLALVAASLALVPVNALWGPASIDDRLA
CCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VSLALVASLAGLSLAVLWLKRWPTRTESVLFAAIGSATIAGGCLWQAEPLIGLTFCTALA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCEEECCCCHHHHHHHHHHH
VSGGYIAFFHTARYMLMNFVLAVGVGAWQAIRVAAEGEPVLALTGYFLVLELNIGVPFAI
HCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCEEEEEEEEEEEEECCCCCHHH
QVVVRSLGTDLLQSDRDPLTGLLNRRAFTHAVVGRLVARADRAHLAVALIDLDRFKAIND
HHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEHHHHHHCCC
RHGHASGDAALVDVAGALTAAVTETALVCRMGGEEFLIADIVASAAPTEWAQGLCAAVTS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCC
TPFRLTASVGIATVPLRDVKPEQADETFRRLVTEADAAMYAAKRRGGNQIQHAGDARTPR
CCEEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]