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The map label for this gene is fdxH [H]

Identifier: 119871335

GI number: 119871335

Start: 5547686

End: 5548588

Strand: Direct

Name: fdxH [H]

Synonym: Mkms_5309

Alternate gene names: 119871335

Gene position: 5547686-5548588 (Clockwise)

Preceding gene: 119871334

Following gene: 119871336

Centisome position: 96.7

GC content: 68.11

Gene sequence:

>903_bases
ATGCGCGAGAACAGCTTCTACGGTCCGCTCGAGGATCCGGCCGGTGACGCGGGATATTCGGAGCACCCGCAGCGGGTCGG
GTTCTTCACCGACACCTCGGTGTGCATCGGCTGCAAAGCCTGCGAGGTCGCGTGCAAAGAGTGGAACGAGGTGCCCGTCT
CAGGGGAAGGGGACGGGTTCAACCTGCTGGGCATGTCGTTCGACAACACCGGTGAACTGGGTGCGAACTCATGGCGGCAC
GTGGCGTTCATCGAACAGCCGGTCCCCCAACGCGAAACGGTCGATCTCGGCATGCCCGACATCGGCGGTGCGGGCATCGA
CATGGTGGGCGAGCGGCCCGACTTCCGGTGGCTGATGTCCTCCGACGTCTGCAAGCACTGCACGCACGCGGGCTGCCTCG
ACGTGTGCCCGACCGGCGCGCTGTTCCGCACCGAGTTCTCGACCGTCGTTGTGCAGCAGGACATCTGCAATGGCTGCGGG
TACTGCGTCTCGGGCTGCCCGTACGGGGTGATCGAACGGCGCGAGGGCGACGGTCGGGCGTGGAAGTGCACGCTGTGCTA
CGACCGGCTGCACGACGGACTCGAACCCGCGTGCGCCAAGGCCTGCCCGACCGATTCGATCCAGTTCGGCCCGCTCGACG
AACTGCGGGAACGCGCGGCGCTGCGGGTCGAGCAGCTGCACGAGCGCGGGGTGAGCGAGGCCCGGCTGTACGGCCATGAC
CCGAACGACGGGGTGGGCGGTGACGGCGCCTTCTTCCTGCTGCTCGACGAACCGGAAGTGTATGGGCTGCCGCCGGATCC
GGTGGTCCCCACCCGTGACGCCGCCGCGATGTGGCGGTATGCGGGGATGGCGGCGTCGGCCCTGGTCGGTATCGCGGTCT
CGGCCTTCACGAGGCGCTCATGA

Upstream 100 bases:

>100_bases
AGTACATCGCGATGTACCGCGATCGGGCCCGCATCACCGTCGACACCGGGACCGACCTCGACACGACGAAACGGTCGACC
GAACACACCGAGGAAACGTG

Downstream 100 bases:

>100_bases
AGGAGCGCCTGGCCGTACCGAAGGCCGAGTTCCGCTCCTATTACGGTCGGCAGATCCTCAAGACGCCGGTCTGGAACTGG
ATGATCGCCGCCTATCTGTT

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Formate dehydrogenase subunit beta; FDH subunit beta [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRENSFYGPLEDPAGDAGYSEHPQRVGFFTDTSVCIGCKACEVACKEWNEVPVSGEGDGFNLLGMSFDNTGELGANSWRH
VAFIEQPVPQRETVDLGMPDIGGAGIDMVGERPDFRWLMSSDVCKHCTHAGCLDVCPTGALFRTEFSTVVVQQDICNGCG
YCVSGCPYGVIERREGDGRAWKCTLCYDRLHDGLEPACAKACPTDSIQFGPLDELRERAALRVEQLHERGVSEARLYGHD
PNDGVGGDGAFFLLLDEPEVYGLPPDPVVPTRDAAAMWRYAGMAASALVGIAVSAFTRRS

Sequences:

>Translated_300_residues
MRENSFYGPLEDPAGDAGYSEHPQRVGFFTDTSVCIGCKACEVACKEWNEVPVSGEGDGFNLLGMSFDNTGELGANSWRH
VAFIEQPVPQRETVDLGMPDIGGAGIDMVGERPDFRWLMSSDVCKHCTHAGCLDVCPTGALFRTEFSTVVVQQDICNGCG
YCVSGCPYGVIERREGDGRAWKCTLCYDRLHDGLEPACAKACPTDSIQFGPLDELRERAALRVEQLHERGVSEARLYGHD
PNDGVGGDGAFFLLLDEPEVYGLPPDPVVPTRDAAAMWRYAGMAASALVGIAVSAFTRRS
>Mature_300_residues
MRENSFYGPLEDPAGDAGYSEHPQRVGFFTDTSVCIGCKACEVACKEWNEVPVSGEGDGFNLLGMSFDNTGELGANSWRH
VAFIEQPVPQRETVDLGMPDIGGAGIDMVGERPDFRWLMSSDVCKHCTHAGCLDVCPTGALFRTEFSTVVVQQDICNGCG
YCVSGCPYGVIERREGDGRAWKCTLCYDRLHDGLEPACAKACPTDSIQFGPLDELRERAALRVEQLHERGVSEARLYGHD
PNDGVGGDGAFFLLLDEPEVYGLPPDPVVPTRDAAAMWRYAGMAASALVGIAVSAFTRRS

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=279, Percent_Identity=36.2007168458781, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1790326, Length=260, Percent_Identity=36.5384615384615, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787122, Length=194, Percent_Identity=34.020618556701, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787872, Length=194, Percent_Identity=32.4742268041237, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI2367345, Length=192, Percent_Identity=36.4583333333333, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI226510944, Length=210, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789370, Length=208, Percent_Identity=26.4423076923077, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787740, Length=142, Percent_Identity=33.0985915492958, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787478, Length=81, Percent_Identity=37.037037037037, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI2367245, Length=197, Percent_Identity=28.4263959390863, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082179, Length=155, Percent_Identity=30.3225806451613, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789067, Length=208, Percent_Identity=29.8076923076923, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 32515; Mature: 32515

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRENSFYGPLEDPAGDAGYSEHPQRVGFFTDTSVCIGCKACEVACKEWNEVPVSGEGDGF
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCCCCCCCE
NLLGMSFDNTGELGANSWRHVAFIEQPVPQRETVDLGMPDIGGAGIDMVGERPDFRWLMS
EEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEHHH
SDVCKHCTHAGCLDVCPTGALFRTEFSTVVVQQDICNGCGYCVSGCPYGVIERREGDGRA
HHHHHHHHCCCCHHHCCCCCEEHHHHHHHEEHHHHHCCCCHHHCCCCCCEEEEECCCCCE
WKCTLCYDRLHDGLEPACAKACPTDSIQFGPLDELRERAALRVEQLHERGVSEARLYGHD
EEEEEEHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCC
PNDGVGGDGAFFLLLDEPEVYGLPPDPVVPTRDAAAMWRYAGMAASALVGIAVSAFTRRS
CCCCCCCCCEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRENSFYGPLEDPAGDAGYSEHPQRVGFFTDTSVCIGCKACEVACKEWNEVPVSGEGDGF
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCCCCCCCE
NLLGMSFDNTGELGANSWRHVAFIEQPVPQRETVDLGMPDIGGAGIDMVGERPDFRWLMS
EEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEHHH
SDVCKHCTHAGCLDVCPTGALFRTEFSTVVVQQDICNGCGYCVSGCPYGVIERREGDGRA
HHHHHHHHCCCCHHHCCCCCEEHHHHHHHEEHHHHHCCCCHHHCCCCCCEEEEECCCCCE
WKCTLCYDRLHDGLEPACAKACPTDSIQFGPLDELRERAALRVEQLHERGVSEARLYGHD
EEEEEEHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCC
PNDGVGGDGAFFLLLDEPEVYGLPPDPVVPTRDAAAMWRYAGMAASALVGIAVSAFTRRS
CCCCCCCCCEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]