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The map label for this gene is glnT [H]

Identifier: 119871008

GI number: 119871008

Start: 5220256

End: 5221566

Strand: Direct

Name: glnT [H]

Synonym: Mkms_4981

Alternate gene names: 119871008

Gene position: 5220256-5221566 (Clockwise)

Preceding gene: 119871007

Following gene: 119871009

Centisome position: 90.99

GC content: 69.11

Gene sequence:

>1311_bases
ATGTCCACCGATCTCGCCGCCCTCGCCGAGAAGACCGGCACCAAGTTCATCCTCGCGCTGTTCGTCGATCTGCGCGGAAA
GCCCTGCGCCAAGCTCGTTCCCGTCGAAGCGGTCGAACAGCTCGCCACCGAGGGCGTCGGCTTCGCCGGATACGCGGTCG
GGGCCATGGGTCAGGAACCCAAAGACCCTGACCTGATGGCGATTCCGGACGCCGCGTCGTTCACCCCGATCCCGTTCGTC
AAGGACGGCCTGGCGATCGTGCACTGCGATCCGCATGTCGAGGGCAAGCCGTGGCCATACGCGCCGCGCATCATCCTCAG
GAGCCTCATTCAGCAGGCCGCCGACGCCGGCTTCGAACCCTGGGTCGGCGCGGAGGTCGAGTACTTCCTGCTCACCCGCA
ACGCCGACGGCAGCCTCGCCACGGCCGACGCCGCCGACACCGCCGCCCAGCCGTGTTACGACGCGCGCGGGGTGACCCGC
ATGTACGACCACCTCACCACGATCTCCTCGGCGATGAACCAGCTCGGCTGGGCCAACTACGCCAACGACCACGAGGACGG
CAACGGCCAGTTCGAGCAGAATTTCCAGTTCGCCGATGCGTTGACCACCGCCGACCGCGTGATCACGCTGCGCTACCTGC
TGTCGATGATCGCCGCCGAGCGCGGGATGGTGGCCACGTTCATGCCGAAACCGTTCGGGGACCGGACCGGCAGCGGCCTG
CACCTGCACCTGTCGCTGACCAGCGGCGGCACCCCGGTCTTCCCCTGCGAATCCGACGAACGGGGCCTGGGCCTGTCGGA
CACCGCGTACTCCTTCATCGGGGGCATCCTCGACCACGCCTGTGCGCTGCAGTCGGTGATCGCACCGACGGTCAACTCCT
ACAAGCGAACCGGGGCCGTCAGTACCGCCTCCGGTGCGTCCTGGGCGCCGCGCAGCGCCACCTTCGGCGGCAACGACCGC
ACCCACTACATCCGCGTACCCGACGCCCAGCGCATCGAACTGCGCGGCGGTGACGGCTCGGCCAATCCGTACCTCGCGGT
CGCGGCGGCGCTCGGCGCCGGACTGGACGGGATCAAGCGGTCGGCCGATCCGGGCGCGGTCGGCGCGGTGGCGGAGACCC
GGTCGACGCTGCCGCCCACCCTGCTGCACGCGGTCGAGGAACTCGAGGCCGATCCCGTCGTCTCCGGTGTCCTCGACGCC
GCCGGTGACGGCGTCGCACGCTATTTCGCCGGCCTCAAGCGGCACGAATTCTTCGACTACCACGGCACGGTCAGCCCGTG
GGAGGTCGAGCAGTACCTGACGGCGTTCTGA

Upstream 100 bases:

>100_bases
CGGCACGGGGCTGCCCAAGCCTCGTGTCGGCCGCATTCGCATATCCATCGAGTTTCCCCTCGTGATACATTGATTCATCA
CAAGAGACAGAGGTTCTGCC

Downstream 100 bases:

>100_bases
AGTCCTGACAACGAAAGAGAGCGACACATGTGTGGGATCGTCGGGTTGCACCTGCGCAACCCCGAACTCCAACCGCGGCT
GGGCGAACTGCTCACCGGAA

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSTDLAALAEKTGTKFILALFVDLRGKPCAKLVPVEAVEQLATEGVGFAGYAVGAMGQEPKDPDLMAIPDAASFTPIPFV
KDGLAIVHCDPHVEGKPWPYAPRIILRSLIQQAADAGFEPWVGAEVEYFLLTRNADGSLATADAADTAAQPCYDARGVTR
MYDHLTTISSAMNQLGWANYANDHEDGNGQFEQNFQFADALTTADRVITLRYLLSMIAAERGMVATFMPKPFGDRTGSGL
HLHLSLTSGGTPVFPCESDERGLGLSDTAYSFIGGILDHACALQSVIAPTVNSYKRTGAVSTASGASWAPRSATFGGNDR
THYIRVPDAQRIELRGGDGSANPYLAVAAALGAGLDGIKRSADPGAVGAVAETRSTLPPTLLHAVEELEADPVVSGVLDA
AGDGVARYFAGLKRHEFFDYHGTVSPWEVEQYLTAF

Sequences:

>Translated_436_residues
MSTDLAALAEKTGTKFILALFVDLRGKPCAKLVPVEAVEQLATEGVGFAGYAVGAMGQEPKDPDLMAIPDAASFTPIPFV
KDGLAIVHCDPHVEGKPWPYAPRIILRSLIQQAADAGFEPWVGAEVEYFLLTRNADGSLATADAADTAAQPCYDARGVTR
MYDHLTTISSAMNQLGWANYANDHEDGNGQFEQNFQFADALTTADRVITLRYLLSMIAAERGMVATFMPKPFGDRTGSGL
HLHLSLTSGGTPVFPCESDERGLGLSDTAYSFIGGILDHACALQSVIAPTVNSYKRTGAVSTASGASWAPRSATFGGNDR
THYIRVPDAQRIELRGGDGSANPYLAVAAALGAGLDGIKRSADPGAVGAVAETRSTLPPTLLHAVEELEADPVVSGVLDA
AGDGVARYFAGLKRHEFFDYHGTVSPWEVEQYLTAF
>Mature_435_residues
STDLAALAEKTGTKFILALFVDLRGKPCAKLVPVEAVEQLATEGVGFAGYAVGAMGQEPKDPDLMAIPDAASFTPIPFVK
DGLAIVHCDPHVEGKPWPYAPRIILRSLIQQAADAGFEPWVGAEVEYFLLTRNADGSLATADAADTAAQPCYDARGVTRM
YDHLTTISSAMNQLGWANYANDHEDGNGQFEQNFQFADALTTADRVITLRYLLSMIAAERGMVATFMPKPFGDRTGSGLH
LHLSLTSGGTPVFPCESDERGLGLSDTAYSFIGGILDHACALQSVIAPTVNSYKRTGAVSTASGASWAPRSATFGGNDRT
HYIRVPDAQRIELRGGDGSANPYLAVAAALGAGLDGIKRSADPGAVGAVAETRSTLPPTLLHAVEELEADPVVSGVLDAA
GDGVARYFAGLKRHEFFDYHGTVSPWEVEQYLTAF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=423, Percent_Identity=31.4420803782506, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87081870, Length=378, Percent_Identity=30.1587301587302, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 46228; Mature: 46097

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDLAALAEKTGTKFILALFVDLRGKPCAKLVPVEAVEQLATEGVGFAGYAVGAMGQEP
CCCHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCC
KDPDLMAIPDAASFTPIPFVKDGLAIVHCDPHVEGKPWPYAPRIILRSLIQQAADAGFEP
CCCCEEECCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
WVGAEVEYFLLTRNADGSLATADAADTAAQPCYDARGVTRMYDHLTTISSAMNQLGWANY
CCCCCEEEEEEEECCCCCEEECCCHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCC
ANDHEDGNGQFEQNFQFADALTTADRVITLRYLLSMIAAERGMVATFMPKPFGDRTGSGL
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCE
HLHLSLTSGGTPVFPCESDERGLGLSDTAYSFIGGILDHACALQSVIAPTVNSYKRTGAV
EEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STASGASWAPRSATFGGNDRTHYIRVPDAQRIELRGGDGSANPYLAVAAALGAGLDGIKR
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHC
SADPGAVGAVAETRSTLPPTLLHAVEELEADPVVSGVLDAAGDGVARYFAGLKRHEFFDY
CCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
HGTVSPWEVEQYLTAF
CCCCCHHHHHHHHHCC
>Mature Secondary Structure 
STDLAALAEKTGTKFILALFVDLRGKPCAKLVPVEAVEQLATEGVGFAGYAVGAMGQEP
CCHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCC
KDPDLMAIPDAASFTPIPFVKDGLAIVHCDPHVEGKPWPYAPRIILRSLIQQAADAGFEP
CCCCEEECCCCCCCCCCCEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
WVGAEVEYFLLTRNADGSLATADAADTAAQPCYDARGVTRMYDHLTTISSAMNQLGWANY
CCCCCEEEEEEEECCCCCEEECCCHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCC
ANDHEDGNGQFEQNFQFADALTTADRVITLRYLLSMIAAERGMVATFMPKPFGDRTGSGL
CCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCE
HLHLSLTSGGTPVFPCESDERGLGLSDTAYSFIGGILDHACALQSVIAPTVNSYKRTGAV
EEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STASGASWAPRSATFGGNDRTHYIRVPDAQRIELRGGDGSANPYLAVAAALGAGLDGIKR
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHC
SADPGAVGAVAETRSTLPPTLLHAVEELEADPVVSGVLDAAGDGVARYFAGLKRHEFFDY
CCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
HGTVSPWEVEQYLTAF
CCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]