Click here to switch to the map view.

The map label for this gene is amt [H]

Identifier: 119871007

GI number: 119871007

Start: 5218888

End: 5220141

Strand: Direct

Name: amt [H]

Synonym: Mkms_4980

Alternate gene names: 119871007

Gene position: 5218888-5220141 (Clockwise)

Preceding gene: 119871006

Following gene: 119871008

Centisome position: 90.97

GC content: 66.91

Gene sequence:

>1254_bases
ATGGATACAGGAACCACAGCCTTCATGCTGTGTTGCATCATCGGCCTCACGCTGATGATCCCCGGTCTCGCCCTGTTCTA
CGGCGGCATGGTCTCCGTGAAGAGCTCGACCAACATGATGATGATGACCTTCGGGGCCGTCGCAGCCGTCGGGCTGCTGT
GGGTCCTGTTCGGCTTCTCCATGGTGTTCGGCACCTCGTACGGAGGGTTCGTCGGCAGCTTCACCGAGTTCGCCGGCATG
ACGGACCTGCTGGAGCCGATGACCACCGTCGACGGTCTGCCGATCAGCCTCTTCGCCGTCTTCCAGGCGCTGTTCGCCGC
GATCACCGTCGCGCTGATCTCCGGTGCCGTCGCCGACCGGATGAAGTTCGGCGCCTGGATGGGCTTCGCCACGCTGTGGG
CGGTGCTCGTGTACTTCCCCGTCGCACACTGGGTCTTCGCATTCGACGGCGTCGTGACCGAGAACTCGGTCGGCGGCTGG
ATCGCCAACACCCTGGGCGCCGTCGACTTCGCCGGTGGCACCGCGGTGCACATCAACGCCGGTGCCGCCGCGCTGGCCGT
GGCGATCGTGCTCGGCAAATCCGCGATGTTCGGCCAGCTGCGCAAACCGCACAACGTGCCGCTGACGCTGCTGGGCGCCG
GACTGCTGTGGGCCGGCTGGTATGCGTTCAACGGCGGTTCCGCACTGGCCGCGCGCAATTCGGCCGCCATCGTCATGGTC
ACAACGTTCGTCGCCACCTGCGCGGCGGTGCTGGCCTGGCTGGCCGTGGAGAAGATCAAGGACGGCCACGTCACCGGCGT
CGGCGCGGCCTCGGGCGCCATCACCGGCCTGGTCGCGATCACCCCCGCCTGCGGTGCGGTCACCCCGATCGGCGCGATCT
TCGTCGGCGGTATCGCCGGCGCGGTGTGCGTGTACGCGGTCGGCCTCAAAACCCGCTTCGGCTATGACGATTCACTCGAC
GTGGTCGGTGTTCACCTCGTCGGCGGCGTGATCGGCACCCTGCTGATCGGCTTCTTCGCCAGCGAGGGGATGCCGAACGG
CGTCAACGGGCTGTTCTACGGCGGCGGAATCGACCAGCTGTGGAAGCAGGCTGTCGCGGCCGGTGCGGTGATGCTCTACT
CGTTCGTGGTGGCCTACGTCATCGCGTTCGCGCTGAAGAAGACCGTCGGCATCCGCGTCTCCCCCGACGAGGAGGAGCAG
GGCATCGACGCGAAGTTCCACCGCGAGTCCTCGTACGACTGGCAACCCGCCTAG

Upstream 100 bases:

>100_bases
ACGCCGTTGCAACACAAACCAACCTGTCTCTGTAACAGAAACATCGGTTACTGCCAGTGATCGGCCTGCCGTGGGCCGCT
TACCGCAGAAAAGGTTTCAC

Downstream 100 bases:

>100_bases
TCACCACCCGCGGCCGGCACGGGGCTGCCCAAGCCTCGTGTCGGCCGCATTCGCATATCCATCGAGTTTCCCCTCGTGAT
ACATTGATTCATCACAAGAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MDTGTTAFMLCCIIGLTLMIPGLALFYGGMVSVKSSTNMMMMTFGAVAAVGLLWVLFGFSMVFGTSYGGFVGSFTEFAGM
TDLLEPMTTVDGLPISLFAVFQALFAAITVALISGAVADRMKFGAWMGFATLWAVLVYFPVAHWVFAFDGVVTENSVGGW
IANTLGAVDFAGGTAVHINAGAAALAVAIVLGKSAMFGQLRKPHNVPLTLLGAGLLWAGWYAFNGGSALAARNSAAIVMV
TTFVATCAAVLAWLAVEKIKDGHVTGVGAASGAITGLVAITPACGAVTPIGAIFVGGIAGAVCVYAVGLKTRFGYDDSLD
VVGVHLVGGVIGTLLIGFFASEGMPNGVNGLFYGGGIDQLWKQAVAAGAVMLYSFVVAYVIAFALKKTVGIRVSPDEEEQ
GIDAKFHRESSYDWQPA

Sequences:

>Translated_417_residues
MDTGTTAFMLCCIIGLTLMIPGLALFYGGMVSVKSSTNMMMMTFGAVAAVGLLWVLFGFSMVFGTSYGGFVGSFTEFAGM
TDLLEPMTTVDGLPISLFAVFQALFAAITVALISGAVADRMKFGAWMGFATLWAVLVYFPVAHWVFAFDGVVTENSVGGW
IANTLGAVDFAGGTAVHINAGAAALAVAIVLGKSAMFGQLRKPHNVPLTLLGAGLLWAGWYAFNGGSALAARNSAAIVMV
TTFVATCAAVLAWLAVEKIKDGHVTGVGAASGAITGLVAITPACGAVTPIGAIFVGGIAGAVCVYAVGLKTRFGYDDSLD
VVGVHLVGGVIGTLLIGFFASEGMPNGVNGLFYGGGIDQLWKQAVAAGAVMLYSFVVAYVIAFALKKTVGIRVSPDEEEQ
GIDAKFHRESSYDWQPA
>Mature_417_residues
MDTGTTAFMLCCIIGLTLMIPGLALFYGGMVSVKSSTNMMMMTFGAVAAVGLLWVLFGFSMVFGTSYGGFVGSFTEFAGM
TDLLEPMTTVDGLPISLFAVFQALFAAITVALISGAVADRMKFGAWMGFATLWAVLVYFPVAHWVFAFDGVVTENSVGGW
IANTLGAVDFAGGTAVHINAGAAALAVAIVLGKSAMFGQLRKPHNVPLTLLGAGLLWAGWYAFNGGSALAARNSAAIVMV
TTFVATCAAVLAWLAVEKIKDGHVTGVGAASGAITGLVAITPACGAVTPIGAIFVGGIAGAVCVYAVGLKTRFGYDDSLD
VVGVHLVGGVIGTLLIGFFASEGMPNGVNGLFYGGGIDQLWKQAVAAGAVMLYSFVVAYVIAFALKKTVGIRVSPDEEEQ
GIDAKFHRESSYDWQPA

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=418, Percent_Identity=37.799043062201, Blast_Score=238, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI32564042, Length=419, Percent_Identity=26.7303102625298, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI133931100, Length=420, Percent_Identity=27.6190476190476, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17550182, Length=437, Percent_Identity=28.604118993135, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17550180, Length=438, Percent_Identity=27.3972602739726, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=417, Percent_Identity=35.0119904076739, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6325396, Length=424, Percent_Identity=30.6603773584906, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321559, Length=423, Percent_Identity=30.0236406619385, Blast_Score=176, Evalue=9e-45,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=30.9178743961353, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43024; Mature: 43024

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGTTAFMLCCIIGLTLMIPGLALFYGGMVSVKSSTNMMMMTFGAVAAVGLLWVLFGFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
MVFGTSYGGFVGSFTEFAGMTDLLEPMTTVDGLPISLFAVFQALFAAITVALISGAVADR
HHHHCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MKFGAWMGFATLWAVLVYFPVAHWVFAFDGVVTENSVGGWIANTLGAVDFAGGTAVHINA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHCCCCCCCCCEEEEEC
GAAALAVAIVLGKSAMFGQLRKPHNVPLTLLGAGLLWAGWYAFNGGSALAARNSAAIVMV
CHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCEEEHH
TTFVATCAAVLAWLAVEKIKDGHVTGVGAASGAITGLVAITPACGAVTPIGAIFVGGIAG
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AVCVYAVGLKTRFGYDDSLDVVGVHLVGGVIGTLLIGFFASEGMPNGVNGLFYGGGIDQL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHH
WKQAVAAGAVMLYSFVVAYVIAFALKKTVGIRVSPDEEEQGIDAKFHRESSYDWQPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MDTGTTAFMLCCIIGLTLMIPGLALFYGGMVSVKSSTNMMMMTFGAVAAVGLLWVLFGFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
MVFGTSYGGFVGSFTEFAGMTDLLEPMTTVDGLPISLFAVFQALFAAITVALISGAVADR
HHHHCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MKFGAWMGFATLWAVLVYFPVAHWVFAFDGVVTENSVGGWIANTLGAVDFAGGTAVHINA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHCCCCCCCCCEEEEEC
GAAALAVAIVLGKSAMFGQLRKPHNVPLTLLGAGLLWAGWYAFNGGSALAARNSAAIVMV
CHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCEEEHH
TTFVATCAAVLAWLAVEKIKDGHVTGVGAASGAITGLVAITPACGAVTPIGAIFVGGIAG
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AVCVYAVGLKTRFGYDDSLDVVGVHLVGGVIGTLLIGFFASEGMPNGVNGLFYGGGIDQL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHH
WKQAVAAGAVMLYSFVVAYVIAFALKKTVGIRVSPDEEEQGIDAKFHRESSYDWQPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]