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The map label for this gene is ydfA [H]

Identifier: 119870849

GI number: 119870849

Start: 5050545

End: 5051780

Strand: Direct

Name: ydfA [H]

Synonym: Mkms_4821

Alternate gene names: 119870849

Gene position: 5050545-5051780 (Clockwise)

Preceding gene: 119870847

Following gene: 119870855

Centisome position: 88.03

GC content: 70.47

Gene sequence:

>1236_bases
TTGCTTCTCGCGCTACTGCTGCTCGTCGTCGTACTGGCGTTCGCCCTCGCGCGGCCCCGCGGGTGGCCCGAAGCGGTGGC
CGCGGTGCCCGCCGCCGGCCTGCTCGTCGCCACCGGGGCGTTGTCGGTCGACGAGGCGGTCGCCGAGATCACCCGGCTGG
CGCCGGTCGTCGGCTTCCTGGCCGCGGTGTTGGTGCTGGCGCAACTCTGCGACGACGAGGGTCTGTTCCACGCGGCCGGG
GTGCTCATGGCCCGCCGCGGAGCCGGCGATCCCCGGCGGCTGCTGACCGTCGTATTCGTCGTCGCCTCCGCCACCACCGC
GATCCTGAGCCTGGACGCCACCGTGGTCCTGCTCACGCCGGTGGTGCTCGCCACCGCTCGCACCCTGTCCATCCCCGCCG
CGCCGCACGTCTATGCCACCGCCCACCTGGCCAACAGCGCCTCGCTGCTGCTGCCGGTGTCCAACCTGACCAACCTGCTC
GCATTCACCGCCGCCGGCCTGTCGTTCACCCATTTCGCCGCGCTGATGACACTGCCGTGGCTGGCGGCGATCGTCGGTGA
ATACATCCTGCTGCGAGCGCTTTTCCGGAACGATCTGGCAGCCGTCCCGGCGCCCGACAAGACCGTGCCCGCCCCGGAAC
TGCCGCTGTTCGTCCTCGTGGTCCTCGGACTCACGCTCGGCGGATTCGCGGTCACCTCGCTGCTGGGACTGTCGCCGGCC
TGGGCGGCACTGGCCGGTGCGGTTGTCCTCGGCGTCCGCAGCCTCGCGCAGCGCCGCAGCACCGTCGCCGGAATCGTGCG
GTCGGCGAACGTGCCGTTCCTGGCCTTCGTGCTGTGCCTGGGTGTGGTGGTCGAGGCCGTCATGGTGCACGGCCTCGACG
ACGCGATGCGACAGGCGCTCCCGGCCGGTGACGGCCTGGTGGCGCTGTTGGGCATTGCGGCCGTTGCGGCGGTGCTGTCG
AATGTCGTCAACAACGTGCCTGCGGTGCTGTTACTGCTGCCGTTGGTGGCCGGGGCGGGTCCCGCAGCGGTGCTGGCGGT
GCTGATCGGGGTGAACATCGGCCCGAACCTGACCTACGTCGGATCGTTGGCGAACCTGCTGTGGCGCAGCATTGTTCGTG
GCGAGAACATCCCAGCCGGGGTGCTGCAGTTCAGCCGGGTGGGGTTGTGCACGGTGCCGCTCACACTGGTCGCATCGGTG
TTCGGGCTGTGGGCCGGTCTTGCGCTGTTCACCTGA

Upstream 100 bases:

>100_bases
TGATGGTCGAGGTTAGCGAGCGAGGCAGGCGCCGACATGCGTATGCGGCACCGCGGATCGATTCGTCATCGAATGTTCGG
CAGTCGGAAAGGACCTGCGG

Downstream 100 bases:

>100_bases
TGATCCGCTGAGTTCACCCACTGGTCGGGCGCTGCGACGAGCGCACCGCGGCGATCCTGCGGACAATCGAACACTGTGGC
GCAGCGGGCTGACGAGAGTG

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MLLALLLLVVVLAFALARPRGWPEAVAAVPAAGLLVATGALSVDEAVAEITRLAPVVGFLAAVLVLAQLCDDEGLFHAAG
VLMARRGAGDPRRLLTVVFVVASATTAILSLDATVVLLTPVVLATARTLSIPAAPHVYATAHLANSASLLLPVSNLTNLL
AFTAAGLSFTHFAALMTLPWLAAIVGEYILLRALFRNDLAAVPAPDKTVPAPELPLFVLVVLGLTLGGFAVTSLLGLSPA
WAALAGAVVLGVRSLAQRRSTVAGIVRSANVPFLAFVLCLGVVVEAVMVHGLDDAMRQALPAGDGLVALLGIAAVAAVLS
NVVNNVPAVLLLLPLVAGAGPAAVLAVLIGVNIGPNLTYVGSLANLLWRSIVRGENIPAGVLQFSRVGLCTVPLTLVASV
FGLWAGLALFT

Sequences:

>Translated_411_residues
MLLALLLLVVVLAFALARPRGWPEAVAAVPAAGLLVATGALSVDEAVAEITRLAPVVGFLAAVLVLAQLCDDEGLFHAAG
VLMARRGAGDPRRLLTVVFVVASATTAILSLDATVVLLTPVVLATARTLSIPAAPHVYATAHLANSASLLLPVSNLTNLL
AFTAAGLSFTHFAALMTLPWLAAIVGEYILLRALFRNDLAAVPAPDKTVPAPELPLFVLVVLGLTLGGFAVTSLLGLSPA
WAALAGAVVLGVRSLAQRRSTVAGIVRSANVPFLAFVLCLGVVVEAVMVHGLDDAMRQALPAGDGLVALLGIAAVAAVLS
NVVNNVPAVLLLLPLVAGAGPAAVLAVLIGVNIGPNLTYVGSLANLLWRSIVRGENIPAGVLQFSRVGLCTVPLTLVASV
FGLWAGLALFT
>Mature_411_residues
MLLALLLLVVVLAFALARPRGWPEAVAAVPAAGLLVATGALSVDEAVAEITRLAPVVGFLAAVLVLAQLCDDEGLFHAAG
VLMARRGAGDPRRLLTVVFVVASATTAILSLDATVVLLTPVVLATARTLSIPAAPHVYATAHLANSASLLLPVSNLTNLL
AFTAAGLSFTHFAALMTLPWLAAIVGEYILLRALFRNDLAAVPAPDKTVPAPELPLFVLVVLGLTLGGFAVTSLLGLSPA
WAALAGAVVLGVRSLAQRRSTVAGIVRSANVPFLAFVLCLGVVVEAVMVHGLDDAMRQALPAGDGLVALLGIAAVAAVLS
NVVNNVPAVLLLLPLVAGAGPAAVLAVLIGVNIGPNLTYVGSLANLLWRSIVRGENIPAGVLQFSRVGLCTVPLTLVASV
FGLWAGLALFT

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=416, Percent_Identity=29.3269230769231, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 41828; Mature: 41828

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLALLLLVVVLAFALARPRGWPEAVAAVPAAGLLVATGALSVDEAVAEITRLAPVVGFL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AAVLVLAQLCDDEGLFHAAGVLMARRGAGDPRRLLTVVFVVASATTAILSLDATVVLLTP
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
VVLATARTLSIPAAPHVYATAHLANSASLLLPVSNLTNLLAFTAAGLSFTHFAALMTLPW
HHHHHHHHHCCCCCCCHHHHHHHCCCCHHEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LAAIVGEYILLRALFRNDLAAVPAPDKTVPAPELPLFVLVVLGLTLGGFAVTSLLGLSPA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
WAALAGAVVLGVRSLAQRRSTVAGIVRSANVPFLAFVLCLGVVVEAVMVHGLDDAMRQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
PAGDGLVALLGIAAVAAVLSNVVNNVPAVLLLLPLVAGAGPAAVLAVLIGVNIGPNLTYV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHH
GSLANLLWRSIVRGENIPAGVLQFSRVGLCTVPLTLVASVFGLWAGLALFT
HHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLALLLLVVVLAFALARPRGWPEAVAAVPAAGLLVATGALSVDEAVAEITRLAPVVGFL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AAVLVLAQLCDDEGLFHAAGVLMARRGAGDPRRLLTVVFVVASATTAILSLDATVVLLTP
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
VVLATARTLSIPAAPHVYATAHLANSASLLLPVSNLTNLLAFTAAGLSFTHFAALMTLPW
HHHHHHHHHCCCCCCCHHHHHHHCCCCHHEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LAAIVGEYILLRALFRNDLAAVPAPDKTVPAPELPLFVLVVLGLTLGGFAVTSLLGLSPA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
WAALAGAVVLGVRSLAQRRSTVAGIVRSANVPFLAFVLCLGVVVEAVMVHGLDDAMRQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
PAGDGLVALLGIAAVAAVLSNVVNNVPAVLLLLPLVAGAGPAAVLAVLIGVNIGPNLTYV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHH
GSLANLLWRSIVRGENIPAGVLQFSRVGLCTVPLTLVASVFGLWAGLALFT
HHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]