Click here to switch to the map view.

The map label for this gene is kshB [H]

Identifier: 119870824

GI number: 119870824

Start: 5027888

End: 5028940

Strand: Direct

Name: kshB [H]

Synonym: Mkms_4796

Alternate gene names: 119870824

Gene position: 5027888-5028940 (Clockwise)

Preceding gene: 119870817

Following gene: 119870825

Centisome position: 87.64

GC content: 67.71

Gene sequence:

>1053_bases
GTGACGGACGAGCCACTCGGTAGCCACGTGCTGGAACTGGAGGTGGCCGACGTCGTCGAGGAGACCGCCGACGCACGCTC
CCTGGTGTTCAAGGCGGGTGCCGACATCCCGGCCGAACGGCTGCGCTACTCCCCCGGCCAGTTCCTCACGCTGCGTGTGC
CCAGCGACCGGACCGGATCCGTCGCGCGGTGCTACTCGCTGTGCAGTTCGCCGTTCACCGACGACCCGCTGACCGTGACG
GTCAAGCGCACCGCCGACGGCTACGCCTCGAACTGGTTGTGCGACAACGCCCACCCCGGCATGAAGCTTCACGTGCTGGC
CCCGTCGGGCACCTTCGTCCCGAAATCGCTCGACACCGACTTCCTGCTGATGGCCGCAGGCAGCGGCATCACCCCGATGC
TCGCCATCCTGAAGTCGGCACTGGTCGAGGGCAGCGGGAAGGTGGTGCTGGTCTACGCCAACCGCGACGAATCCAACGTC
ATCTTCGCCGGCACGCTGCGGGAGCTGGGCCAGAAGTACTCCGACCGGCTCACCGTCGTGCACTGGCTGGAATCCGTGCA
GGGTCTGCCCACCGCGACCGCGCTGGCCGGCCTGGCCGCCCCGTACACCGGTCACGACGCCTACATCTGCGGACCGGGTC
CGTTCATGGCCGCCGCCGAGGAGGCGCTGACCAGCGCGGGCACTGCCGCCGACAAGATCCACATCGAGGTCTTCAAATCG
CTGGAGTCCGATCCGTTCGCCGCGGTGGTCATCGAAGAGGACGACAGCGACGAGGGCCCGGCCACCGCTGTGGTGACCCT
CGACGGCGAGACCCACGAGATTCGCTGGCCGCGCAACGCGAAGCTGCTCGACGTACTGCTCGACAAGGGCCTCGACGCGC
CGTTCTCCTGCCGCGAGGGCCATTGCGGCGCCTGCGCGGTGCTCAAGAAGAGCGGCGAGGTCGAGATGGCGGTCAACGAC
GTGCTCGAGCAGTCGGACCTCGACGAGGGCCTCATCCTGGGATGCCAGGCGATGCCTCGTTCGGATTCGGTCGAAGTCAC
TTACGACGAATAG

Upstream 100 bases:

>100_bases
TGTCGAGCAGCGTGACGCTGCAGCGGCTGGACCTGCGAAGGTGCATTTTTGTAACCTGTTCTAGTTCTGCTACTCCGGAC
CGGACAGAAAGGTGCGCGTC

Downstream 100 bases:

>100_bases
CGAAGGGCTAACTTCAACACGATGAACCGGCTCGCAGCGGCAGGTGCGGCGGCACTGCTGACCGCACTGCTCACCCACCC
CGCGACGGCGGCGGCCGCCG

Product: ferredoxin

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTDEPLGSHVLELEVADVVEETADARSLVFKAGADIPAERLRYSPGQFLTLRVPSDRTGSVARCYSLCSSPFTDDPLTVT
VKRTADGYASNWLCDNAHPGMKLHVLAPSGTFVPKSLDTDFLLMAAGSGITPMLAILKSALVEGSGKVVLVYANRDESNV
IFAGTLRELGQKYSDRLTVVHWLESVQGLPTATALAGLAAPYTGHDAYICGPGPFMAAAEEALTSAGTAADKIHIEVFKS
LESDPFAAVVIEEDDSDEGPATAVVTLDGETHEIRWPRNAKLLDVLLDKGLDAPFSCREGHCGACAVLKKSGEVEMAVND
VLEQSDLDEGLILGCQAMPRSDSVEVTYDE

Sequences:

>Translated_350_residues
MTDEPLGSHVLELEVADVVEETADARSLVFKAGADIPAERLRYSPGQFLTLRVPSDRTGSVARCYSLCSSPFTDDPLTVT
VKRTADGYASNWLCDNAHPGMKLHVLAPSGTFVPKSLDTDFLLMAAGSGITPMLAILKSALVEGSGKVVLVYANRDESNV
IFAGTLRELGQKYSDRLTVVHWLESVQGLPTATALAGLAAPYTGHDAYICGPGPFMAAAEEALTSAGTAADKIHIEVFKS
LESDPFAAVVIEEDDSDEGPATAVVTLDGETHEIRWPRNAKLLDVLLDKGLDAPFSCREGHCGACAVLKKSGEVEMAVND
VLEQSDLDEGLILGCQAMPRSDSVEVTYDE
>Mature_349_residues
TDEPLGSHVLELEVADVVEETADARSLVFKAGADIPAERLRYSPGQFLTLRVPSDRTGSVARCYSLCSSPFTDDPLTVTV
KRTADGYASNWLCDNAHPGMKLHVLAPSGTFVPKSLDTDFLLMAAGSGITPMLAILKSALVEGSGKVVLVYANRDESNVI
FAGTLRELGQKYSDRLTVVHWLESVQGLPTATALAGLAAPYTGHDAYICGPGPFMAAAEEALTSAGTAADKIHIEVFKSL
ESDPFAAVVIEEDDSDEGPATAVVTLDGETHEIRWPRNAKLLDVLLDKGLDAPFSCREGHCGACAVLKKSGEVEMAVNDV
LEQSDLDEGLILGCQAMPRSDSVEVTYDE

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=353, Percent_Identity=33.1444759206799, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787098, Length=342, Percent_Identity=25.1461988304094, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788903, Length=211, Percent_Identity=31.2796208530806, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37287; Mature: 37156

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEPLGSHVLELEVADVVEETADARSLVFKAGADIPAERLRYSPGQFLTLRVPSDRTGS
CCCCCCCCEEEEEEHHHHHHHHHHHHHEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCC
VARCYSLCSSPFTDDPLTVTVKRTADGYASNWLCDNAHPGMKLHVLAPSGTFVPKSLDTD
HHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCEEEEEECCCCCCCCCCCCCC
FLLMAAGSGITPMLAILKSALVEGSGKVVLVYANRDESNVIFAGTLRELGQKYSDRLTVV
EEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHH
HWLESVQGLPTATALAGLAAPYTGHDAYICGPGPFMAAAEEALTSAGTAADKIHIEVFKS
HHHHHHCCCCHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCHHEEHHHHHHH
LESDPFAAVVIEEDDSDEGPATAVVTLDGETHEIRWPRNAKLLDVLLDKGLDAPFSCREG
CCCCCEEEEEEECCCCCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCC
HCGACAVLKKSGEVEMAVNDVLEQSDLDEGLILGCQAMPRSDSVEVTYDE
CCCEEEEEECCCCEEEHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECC
>Mature Secondary Structure 
TDEPLGSHVLELEVADVVEETADARSLVFKAGADIPAERLRYSPGQFLTLRVPSDRTGS
CCCCCCCEEEEEEHHHHHHHHHHHHHEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCC
VARCYSLCSSPFTDDPLTVTVKRTADGYASNWLCDNAHPGMKLHVLAPSGTFVPKSLDTD
HHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCEEEEEECCCCCCCCCCCCCC
FLLMAAGSGITPMLAILKSALVEGSGKVVLVYANRDESNVIFAGTLRELGQKYSDRLTVV
EEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHH
HWLESVQGLPTATALAGLAAPYTGHDAYICGPGPFMAAAEEALTSAGTAADKIHIEVFKS
HHHHHHCCCCHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCHHEEHHHHHHH
LESDPFAAVVIEEDDSDEGPATAVVTLDGETHEIRWPRNAKLLDVLLDKGLDAPFSCREG
CCCCCEEEEEEECCCCCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCC
HCGACAVLKKSGEVEMAVNDVLEQSDLDEGLILGCQAMPRSDSVEVTYDE
CCCEEEEEECCCCEEEHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA