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The map label for this gene is lcfB [H]

Identifier: 119870528

GI number: 119870528

Start: 4732041

End: 4733525

Strand: Direct

Name: lcfB [H]

Synonym: Mkms_4499

Alternate gene names: 119870528

Gene position: 4732041-4733525 (Clockwise)

Preceding gene: 119870527

Following gene: 119870531

Centisome position: 82.48

GC content: 68.75

Gene sequence:

>1485_bases
GTGAACCTGTTCGCACTGCTCGACCAGACCGCGGCGCGCCACGCCGACCGCGGCGCGGTGTATCACGGTGAGCGCCAGGT
GCACACGTGGTCGTCGCTGCGGGAGCGGGCGCTGCGGTTGGCGGGGTCGTTCACCGGGTTCGGGCCCGGTGCCCGGATCG
CGGTGGCCAGCGAGAACGCACCCGAGATCGTCGAGTTGATGTTCGGCATCTGGGCGGCCGAAGCCGTGATCGTGCCGGTC
AACTACAAGTTGCATCCGCGGGAGATGGAGCAGATCGTCGCCGACGCCGGGGTCTCCCGGATCTTCGCGTCCCCGAAGAT
CGGCGCGGAGCTGGCACAGCTCGTCGACGTACCGGTCGAGGTGATCGGCGGCGCCGAATACGAAAACCGCTGCGCTGCCT
CACCTGTCGACCCACCACGCACGACCGACCCGGCGTCACTGGCGTGGCTGTTCTACACCAGCGGCACCACGGGCCGCTCC
AAGGGAGCGATGCTGTCGCACCGCAACCTGATGGCGATGACGGTGTCGCATCTGGCCGACTTCGACTCCCCCGACGAGAA
CTGCAGCCTGGTCCACGGCGCGCCCATGTCGCACGGCTCGGGACTGTACGTGCCTCCGTATGTGGCCCGCGGCGCGCGGC
AGGTGGTGCCGGCCTCGGGTGCGTTCGAGCCGGCGGAGTTCCTCGACCTCTGCGAACACCACCCCGGTTGCAGTGCGTTC
CTGGCGCCGACGATGGTGGCCCGACTGGTGGCGACCGGACGGGCCAAACCGGCCAACCTGAACACGATCGTCTACGGCGG
CGGCCCGATGTACGTCGACAGTCTCAAGAAGGCGCTGGCGGCCTTCGGCCCGATCTTCGTGCAGCTCTACGGGCAGGGCG
AGGCGCCGATGACGATCACGGGGCTGCGGCGGTCCGACCACCTCGCCGGCGGTGTTCCGGCCTCCGACGCGGTGCTCGGA
TCGGTCGGGTACGCCCGCTCCGGTGTCGACGTGGCGGTGCTCGGCGAGGACGACCGACCCGCCGGCATCGGTGAGATCGG
CGAGATCGTGTGCCGAGGCGACGTGGTCATGTCCGGTTACTGGAACAACCCGGCGGCCACCGCGGCCACCCTGCAGGACG
GCTGGTTGCGTACCGGGGACATGGGGTCGTTCGACGCCGACGGGTACCTGACGCTGCGCGACCGGTCCAAGGACGTGGTG
ATCAGCGGCGGCAGCAACATCTATCCGCGCGAGGTCGAAGAGATCCTTCTGGAACACCCGGCGGTGGTCGAGGCCGGGGT
GGTGGGGGCGCCGGACGAGGAGTGGGGCGAGATCGTCGTCGCCTTCATCGTCGGCCGGGTCTCCCCCACCGACCTCGATG
CGCATCTGCTGGAACGCATCGCCCGGTTCAAACGACCCAAACGCTACGAGTTCATCGACGAACTGCCGAAGAACAGCTAC
GGCAAGGTTCTCAAGCGGGAGCTGCGGGAGCGGCTCACCACATGA

Upstream 100 bases:

>100_bases
GCGGCAGTTTCATCAACGGCCAGACCATCGTCGTCGACGGTGGCTGGAGTTCGACGAAGTACCTGTCCGATTTCGCGCTG
AACTCCGACTGGGTGCCCAG

Downstream 100 bases:

>100_bases
GTCCGCGGATCAGCTCCGTCGGCGGAACGTCCTCGATCGCGAGTAGACCCGACGGCGCCCGGCACACCCAGTCGATCGCG
TTGACCGCCCGCGCGGCGGT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNLFALLDQTAARHADRGAVYHGERQVHTWSSLRERALRLAGSFTGFGPGARIAVASENAPEIVELMFGIWAAEAVIVPV
NYKLHPREMEQIVADAGVSRIFASPKIGAELAQLVDVPVEVIGGAEYENRCAASPVDPPRTTDPASLAWLFYTSGTTGRS
KGAMLSHRNLMAMTVSHLADFDSPDENCSLVHGAPMSHGSGLYVPPYVARGARQVVPASGAFEPAEFLDLCEHHPGCSAF
LAPTMVARLVATGRAKPANLNTIVYGGGPMYVDSLKKALAAFGPIFVQLYGQGEAPMTITGLRRSDHLAGGVPASDAVLG
SVGYARSGVDVAVLGEDDRPAGIGEIGEIVCRGDVVMSGYWNNPAATAATLQDGWLRTGDMGSFDADGYLTLRDRSKDVV
ISGGSNIYPREVEEILLEHPAVVEAGVVGAPDEEWGEIVVAFIVGRVSPTDLDAHLLERIARFKRPKRYEFIDELPKNSY
GKVLKRELRERLTT

Sequences:

>Translated_494_residues
MNLFALLDQTAARHADRGAVYHGERQVHTWSSLRERALRLAGSFTGFGPGARIAVASENAPEIVELMFGIWAAEAVIVPV
NYKLHPREMEQIVADAGVSRIFASPKIGAELAQLVDVPVEVIGGAEYENRCAASPVDPPRTTDPASLAWLFYTSGTTGRS
KGAMLSHRNLMAMTVSHLADFDSPDENCSLVHGAPMSHGSGLYVPPYVARGARQVVPASGAFEPAEFLDLCEHHPGCSAF
LAPTMVARLVATGRAKPANLNTIVYGGGPMYVDSLKKALAAFGPIFVQLYGQGEAPMTITGLRRSDHLAGGVPASDAVLG
SVGYARSGVDVAVLGEDDRPAGIGEIGEIVCRGDVVMSGYWNNPAATAATLQDGWLRTGDMGSFDADGYLTLRDRSKDVV
ISGGSNIYPREVEEILLEHPAVVEAGVVGAPDEEWGEIVVAFIVGRVSPTDLDAHLLERIARFKRPKRYEFIDELPKNSY
GKVLKRELRERLTT
>Mature_494_residues
MNLFALLDQTAARHADRGAVYHGERQVHTWSSLRERALRLAGSFTGFGPGARIAVASENAPEIVELMFGIWAAEAVIVPV
NYKLHPREMEQIVADAGVSRIFASPKIGAELAQLVDVPVEVIGGAEYENRCAASPVDPPRTTDPASLAWLFYTSGTTGRS
KGAMLSHRNLMAMTVSHLADFDSPDENCSLVHGAPMSHGSGLYVPPYVARGARQVVPASGAFEPAEFLDLCEHHPGCSAF
LAPTMVARLVATGRAKPANLNTIVYGGGPMYVDSLKKALAAFGPIFVQLYGQGEAPMTITGLRRSDHLAGGVPASDAVLG
SVGYARSGVDVAVLGEDDRPAGIGEIGEIVCRGDVVMSGYWNNPAATAATLQDGWLRTGDMGSFDADGYLTLRDRSKDVV
ISGGSNIYPREVEEILLEHPAVVEAGVVGAPDEEWGEIVVAFIVGRVSPTDLDAHLLERIARFKRPKRYEFIDELPKNSY
GKVLKRELRERLTT

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=527, Percent_Identity=26.9449715370019, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI187761343, Length=527, Percent_Identity=26.9449715370019, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI122937307, Length=391, Percent_Identity=29.923273657289, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI42544132, Length=365, Percent_Identity=30.4109589041096, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI156151445, Length=384, Percent_Identity=27.0833333333333, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI58082049, Length=494, Percent_Identity=26.9230769230769, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI157311624, Length=494, Percent_Identity=26.5182186234818, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI157311622, Length=494, Percent_Identity=26.5182186234818, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI38505220, Length=510, Percent_Identity=25.4901960784314, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI115511026, Length=400, Percent_Identity=28.5, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI28416953, Length=416, Percent_Identity=25.2403846153846, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI40807491, Length=193, Percent_Identity=31.6062176165803, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI253970489, Length=378, Percent_Identity=25.6613756613757, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI8923896, Length=378, Percent_Identity=25.6613756613757, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=27.7573529411765, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI145693145, Length=371, Percent_Identity=30.4582210242588, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI221142682, Length=511, Percent_Identity=25.440313111546, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786810, Length=523, Percent_Identity=26.0038240917782, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1788595, Length=351, Percent_Identity=26.7806267806268, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786529, Length=374, Percent_Identity=26.2032085561497, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=488, Percent_Identity=30.7377049180328, Blast_Score=189, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI32563687, Length=489, Percent_Identity=28.8343558282209, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17559526, Length=531, Percent_Identity=27.3069679849341, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17531443, Length=500, Percent_Identity=27, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=382, Percent_Identity=29.8429319371728, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=382, Percent_Identity=29.8429319371728, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=382, Percent_Identity=29.8429319371728, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=490, Percent_Identity=25.3061224489796, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=505, Percent_Identity=24.950495049505, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=460, Percent_Identity=25.6521739130435, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=486, Percent_Identity=24.2798353909465, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=522, Percent_Identity=24.5210727969349, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=425, Percent_Identity=25.8823529411765, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17541856, Length=478, Percent_Identity=24.4769874476987, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17510401, Length=188, Percent_Identity=29.2553191489362, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=383, Percent_Identity=28.7206266318538, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6319264, Length=412, Percent_Identity=26.6990291262136, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=442, Percent_Identity=25.1131221719457, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355181, Length=498, Percent_Identity=27.5100401606426, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24581924, Length=363, Percent_Identity=32.5068870523416, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18859661, Length=513, Percent_Identity=27.4853801169591, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21356947, Length=365, Percent_Identity=27.6712328767123, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI20130357, Length=356, Percent_Identity=26.4044943820225, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648260, Length=355, Percent_Identity=28.7323943661972, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24648257, Length=354, Percent_Identity=29.9435028248588, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653035, Length=355, Percent_Identity=25.3521126760563, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922652, Length=359, Percent_Identity=28.6908077994429, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161076582, Length=361, Percent_Identity=24.9307479224377, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24656500, Length=365, Percent_Identity=26.5753424657534, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21358303, Length=342, Percent_Identity=25.1461988304094, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648253, Length=494, Percent_Identity=20.8502024291498, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648255, Length=348, Percent_Identity=22.7011494252874, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281365686, Length=333, Percent_Identity=26.1261261261261, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21356441, Length=453, Percent_Identity=22.2958057395143, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667959, Length=393, Percent_Identity=26.7175572519084, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24667955, Length=393, Percent_Identity=26.7175572519084, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62472339, Length=393, Percent_Identity=26.7175572519084, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 52975; Mature: 52975

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFALLDQTAARHADRGAVYHGERQVHTWSSLRERALRLAGSFTGFGPGARIAVASENA
CCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
PEIVELMFGIWAAEAVIVPVNYKLHPREMEQIVADAGVSRIFASPKIGAELAQLVDVPVE
HHHHHHHHHHHHCCEEEEEECCEECHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCHH
VIGGAEYENRCAASPVDPPRTTDPASLAWLFYTSGTTGRSKGAMLSHRNLMAMTVSHLAD
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHC
FDSPDENCSLVHGAPMSHGSGLYVPPYVARGARQVVPASGAFEPAEFLDLCEHHPGCSAF
CCCCCCCCEEEECCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCHHHHHHHHHCCCCCHHH
LAPTMVARLVATGRAKPANLNTIVYGGGPMYVDSLKKALAAFGPIFVQLYGQGEAPMTIT
HHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEE
GLRRSDHLAGGVPASDAVLGSVGYARSGVDVAVLGEDDRPAGIGEIGEIVCRGDVVMSGY
ECCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHEEEECCEEEECC
WNNPAATAATLQDGWLRTGDMGSFDADGYLTLRDRSKDVVISGGSNIYPREVEEILLEHP
CCCCCHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHCCC
AVVEAGVVGAPDEEWGEIVVAFIVGRVSPTDLDAHLLERIARFKRPKRYEFIDELPKNSY
CCEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCHH
GKVLKRELRERLTT
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNLFALLDQTAARHADRGAVYHGERQVHTWSSLRERALRLAGSFTGFGPGARIAVASENA
CCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
PEIVELMFGIWAAEAVIVPVNYKLHPREMEQIVADAGVSRIFASPKIGAELAQLVDVPVE
HHHHHHHHHHHHCCEEEEEECCEECHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCHH
VIGGAEYENRCAASPVDPPRTTDPASLAWLFYTSGTTGRSKGAMLSHRNLMAMTVSHLAD
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHC
FDSPDENCSLVHGAPMSHGSGLYVPPYVARGARQVVPASGAFEPAEFLDLCEHHPGCSAF
CCCCCCCCEEEECCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCHHHHHHHHHCCCCCHHH
LAPTMVARLVATGRAKPANLNTIVYGGGPMYVDSLKKALAAFGPIFVQLYGQGEAPMTIT
HHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEE
GLRRSDHLAGGVPASDAVLGSVGYARSGVDVAVLGEDDRPAGIGEIGEIVCRGDVVMSGY
ECCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHEEEECCEEEECC
WNNPAATAATLQDGWLRTGDMGSFDADGYLTLRDRSKDVVISGGSNIYPREVEEILLEHP
CCCCCHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHCCC
AVVEAGVVGAPDEEWGEIVVAFIVGRVSPTDLDAHLLERIARFKRPKRYEFIDELPKNSY
CCEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCHH
GKVLKRELRERLTT
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]