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The map label for this gene is citS [H]

Identifier: 119870273

GI number: 119870273

Start: 4469408

End: 4470985

Strand: Direct

Name: citS [H]

Synonym: Mkms_4243

Alternate gene names: 119870273

Gene position: 4469408-4470985 (Clockwise)

Preceding gene: 119870271

Following gene: 119870274

Centisome position: 77.9

GC content: 70.47

Gene sequence:

>1578_bases
GTGCCGGTGCCCCGTCCCTGGTCCGAGTGGATAGGCCGCTCGCGGCCCCGCTCCCTCGCGGGGCAGGCCATCGCGCTGCA
GATCGTCGTGATCGCGGTCATCGTGGTGGCCGGCAGCACGCTCGCGCTGATCGACGCGCGCCGCGACGGTGACGAGGCGG
CGCGCCAGCAGGTCGTCGGTATCGCGACCGCGCTCGCCGATTCGCCGTCGACCGCTGCGGCCATCGAATCTGGAAGGGCG
ACAGAGCTTCTGCAACCGGTCACCGAAGCGGTCCGCACCCAGACCGGGATCGCGTTCATCACGATCATGGCGCCCGACGG
GGTCCGGTTCACCCACACCGATCCCGGGCAGATCGGCGGCCGCTACCTCGGGACCACCGAACCCGCCCTGCGCGGTGAGA
CGTTCAGCGAGGTCTACACCGGCACGCTGGGTCCCTCGATCCGCGCCGTCGCGCCGGTTCGGGACGGCGACCGCATCGTC
GGACTGGTGTCGGCGGGGATCACGCAACAGACGCTGGCGCAGCGGTGGCGCTCGCAGGTTCCCACGATCGCGGCGGTGAC
CCTTGCGGCAGTGGTCGTTTCGTCGATCGGCATGTGGCTGATCCGGCGGCGCCTGATGCGCCAGACCCACGGCCTGCGGC
CCGACGAACTGCGCGTGATGTACGAACACCACGACGCGATCCTGCATTCGGTGTCCGAGGGTCTGGTGGTGCTCGACCAC
GACGGGGTGGCGCTGGTCAACGACGAGGCGCGCCGCCTGCTCGGCTTGCCGCCCGGGCCGATCGACCGCGCGGCCCTGCC
CCCGTTCCTGCGGACCTCCGATCCCGGGGTGCGCGACGAGGTGCACGTCACCGACGAACGGGTGCTGGTGGTGAACCGTT
CGCGGGTCGCCGACTCCGAGTCGGAGGTGGTGACGATCCGCGACCGCACCGAATTGCAGGGCGCGCTGGGCGAACTCAGC
TCGCTGCAGGTCCTCACCGACTCGCTGCGGGCGCAGGCGCACGAGGCGGCGAACAAGCTGCACACCGTCATCACGATGGT
GGAGATGGGCCGTCCCGAGGACGCGGTGCGCTTCGCGACGCACGAACTCGAATTGTCGCAGCGCCTCGTCGACCGACTGT
CGGAGGCCGTCGCCGAACCCGCGCTGGTGGCACTGCTGCTGGGCAAGACCGCGCAGGCAGACGAACGGGGCATCGCGCTG
ACCGTCACCGAGGACACCCATCTGCCGTCGCACAGCGAAGCGCTGTCGGGCCAGGAGATGGTCACGGTGCTGGGCAATCT
CGTCGACAACGCGATGGACGCCTGCGACCGCGACGACCCGTGGGTGGAGGTGACGGTCAACCAGGACGAGACCCGGTTGT
TGATGCGGGTGGCCGACAGCGGCGCGGGGATGGATCCGGAGACGTTCGCGCGGGCGATGCAGCGCGGCTACTCGACGAAG
CCGGGTGACGGGAAGGCACATCACGGGCTCGGACTGGCGCTCGTCGCGCAGGTGGCGGCCAGGCACGGCGGTACGGTCAC
CGCCGACGTCACCTACGGTTCGGTGGTGACCGTGACGGTGCCGCTGGGAGTCTCGTGA

Upstream 100 bases:

>100_bases
ACGCTCATGGATCCTCCAGGTCGACATCGGGTGCGGTCTGCAAGTAGGTTCGGCCAGGACGTGAGCCAGGTCACGTTTTA
GTTCATTTCGTTCAACGGAG

Downstream 100 bases:

>100_bases
GCACTCGGCCGATCTCGGTGCTCATCGTCGAGGACGATCCGCTGATCGCCGAGGCCCACCAGACCTACCTGTCGCGCCTG
ACCGGTTTCGCCACCGCGGC

Product: signal transduction histidine kinase regulating citrate/malate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MPVPRPWSEWIGRSRPRSLAGQAIALQIVVIAVIVVAGSTLALIDARRDGDEAARQQVVGIATALADSPSTAAAIESGRA
TELLQPVTEAVRTQTGIAFITIMAPDGVRFTHTDPGQIGGRYLGTTEPALRGETFSEVYTGTLGPSIRAVAPVRDGDRIV
GLVSAGITQQTLAQRWRSQVPTIAAVTLAAVVVSSIGMWLIRRRLMRQTHGLRPDELRVMYEHHDAILHSVSEGLVVLDH
DGVALVNDEARRLLGLPPGPIDRAALPPFLRTSDPGVRDEVHVTDERVLVVNRSRVADSESEVVTIRDRTELQGALGELS
SLQVLTDSLRAQAHEAANKLHTVITMVEMGRPEDAVRFATHELELSQRLVDRLSEAVAEPALVALLLGKTAQADERGIAL
TVTEDTHLPSHSEALSGQEMVTVLGNLVDNAMDACDRDDPWVEVTVNQDETRLLMRVADSGAGMDPETFARAMQRGYSTK
PGDGKAHHGLGLALVAQVAARHGGTVTADVTYGSVVTVTVPLGVS

Sequences:

>Translated_525_residues
MPVPRPWSEWIGRSRPRSLAGQAIALQIVVIAVIVVAGSTLALIDARRDGDEAARQQVVGIATALADSPSTAAAIESGRA
TELLQPVTEAVRTQTGIAFITIMAPDGVRFTHTDPGQIGGRYLGTTEPALRGETFSEVYTGTLGPSIRAVAPVRDGDRIV
GLVSAGITQQTLAQRWRSQVPTIAAVTLAAVVVSSIGMWLIRRRLMRQTHGLRPDELRVMYEHHDAILHSVSEGLVVLDH
DGVALVNDEARRLLGLPPGPIDRAALPPFLRTSDPGVRDEVHVTDERVLVVNRSRVADSESEVVTIRDRTELQGALGELS
SLQVLTDSLRAQAHEAANKLHTVITMVEMGRPEDAVRFATHELELSQRLVDRLSEAVAEPALVALLLGKTAQADERGIAL
TVTEDTHLPSHSEALSGQEMVTVLGNLVDNAMDACDRDDPWVEVTVNQDETRLLMRVADSGAGMDPETFARAMQRGYSTK
PGDGKAHHGLGLALVAQVAARHGGTVTADVTYGSVVTVTVPLGVS
>Mature_524_residues
PVPRPWSEWIGRSRPRSLAGQAIALQIVVIAVIVVAGSTLALIDARRDGDEAARQQVVGIATALADSPSTAAAIESGRAT
ELLQPVTEAVRTQTGIAFITIMAPDGVRFTHTDPGQIGGRYLGTTEPALRGETFSEVYTGTLGPSIRAVAPVRDGDRIVG
LVSAGITQQTLAQRWRSQVPTIAAVTLAAVVVSSIGMWLIRRRLMRQTHGLRPDELRVMYEHHDAILHSVSEGLVVLDHD
GVALVNDEARRLLGLPPGPIDRAALPPFLRTSDPGVRDEVHVTDERVLVVNRSRVADSESEVVTIRDRTELQGALGELSS
LQVLTDSLRAQAHEAANKLHTVITMVEMGRPEDAVRFATHELELSQRLVDRLSEAVAEPALVALLLGKTAQADERGIALT
VTEDTHLPSHSEALSGQEMVTVLGNLVDNAMDACDRDDPWVEVTVNQDETRLLMRVADSGAGMDPETFARAMQRGYSTKP
GDGKAHHGLGLALVAQVAARHGGTVTADVTYGSVVTVTVPLGVS

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=524, Percent_Identity=25.763358778626, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790567, Length=526, Percent_Identity=26.425855513308, Blast_Score=168, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56153; Mature: 56021

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPRPWSEWIGRSRPRSLAGQAIALQIVVIAVIVVAGSTLALIDARRDGDEAARQQVVG
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
IATALADSPSTAAAIESGRATELLQPVTEAVRTQTGIAFITIMAPDGVRFTHTDPGQIGG
HHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCHHCCC
RYLGTTEPALRGETFSEVYTGTLGPSIRAVAPVRDGDRIVGLVSAGITQQTLAQRWRSQV
EECCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEEEHHCCCCHHHHHHHHHHCC
PTIAAVTLAAVVVSSIGMWLIRRRLMRQTHGLRPDELRVMYEHHDAILHSVSEGLVVLDH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEC
DGVALVNDEARRLLGLPPGPIDRAALPPFLRTSDPGVRDEVHVTDERVLVVNRSRVADSE
CCEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCC
SEVVTIRDRTELQGALGELSSLQVLTDSLRAQAHEAANKLHTVITMVEMGRPEDAVRFAT
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HELELSQRLVDRLSEAVAEPALVALLLGKTAQADERGIALTVTEDTHLPSHSEALSGQEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHCCHHHH
VTVLGNLVDNAMDACDRDDPWVEVTVNQDETRLLMRVADSGAGMDPETFARAMQRGYSTK
HHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
PGDGKAHHGLGLALVAQVAARHGGTVTADVTYGSVVTVTVPLGVS
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECEEEEEEEECCCC
>Mature Secondary Structure 
PVPRPWSEWIGRSRPRSLAGQAIALQIVVIAVIVVAGSTLALIDARRDGDEAARQQVVG
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
IATALADSPSTAAAIESGRATELLQPVTEAVRTQTGIAFITIMAPDGVRFTHTDPGQIGG
HHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCHHCCC
RYLGTTEPALRGETFSEVYTGTLGPSIRAVAPVRDGDRIVGLVSAGITQQTLAQRWRSQV
EECCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCEEEEEHHCCCCHHHHHHHHHHCC
PTIAAVTLAAVVVSSIGMWLIRRRLMRQTHGLRPDELRVMYEHHDAILHSVSEGLVVLDH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEC
DGVALVNDEARRLLGLPPGPIDRAALPPFLRTSDPGVRDEVHVTDERVLVVNRSRVADSE
CCEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCC
SEVVTIRDRTELQGALGELSSLQVLTDSLRAQAHEAANKLHTVITMVEMGRPEDAVRFAT
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HELELSQRLVDRLSEAVAEPALVALLLGKTAQADERGIALTVTEDTHLPSHSEALSGQEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHCCHHHH
VTVLGNLVDNAMDACDRDDPWVEVTVNQDETRLLMRVADSGAGMDPETFARAMQRGYSTK
HHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
PGDGKAHHGLGLALVAQVAARHGGTVTADVTYGSVVTVTVPLGVS
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]