BacMap

Definition	Mycobacterium sp. KMS chromosome, complete genome.
Accession	NC_008705
Length	5,737,227

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The map label for this gene is echA8 [H]

Identifier: 119870261

GI number: 119870261

Start: 4458527

End: 4459300

Strand: Direct

Name: echA8 [H]

Synonym: Mkms_4231

Alternate gene names: 119870261

Gene position: 4458527-4459300 (Clockwise)

Preceding gene: 119870260

Following gene: 119870262

Centisome position: 77.71

GC content: 67.96

Gene sequence:

>774_bases
ATGGACTACGAGACCATCCTCGTCACCCGCGAGGGCCGGGTCGGCACGATCACGCTGAACCGCCCCAAGGCGCTCAATGC
GCTCAACTCGCAGGTGATGGCCGAAGTCACCACAGCGGCAGCCGAATTCGACTCCGACGAGGGTATCGGCGCGATCGTCG
TCACCGGCAGCGAGAAGGCCTTCGCGGCGGGCGCCGACATCAAGGAGATGGCGAGCCTGTCGTTCGCCGAGGTGTTCACC
TCGGACTTCTTCGCGCTGTGGGGCAAATTCGCCGCCACCCGCACCCCGACCATCGCCGCCGTGGCGGGTTACGCCCTGGG
CGGCGGATGCGAGCTGGCGATGATGTGCGACATCCTCATCGCCGCCGACACCGCGAAGTTCGGCCAGCCCGAGATCAAGC
TGGGCGTGCTGCCCGGCATGGGCGGATCGCAGCGGCTGACCCGCGCGATCGGCAAGGCCAAGGCGATGGACCTGATCCTG
ACCGGCCGCACGATCGGCGCCGAGGAGGCCGAGCGCAGCGGTCTGGTGTCACGGGTGGTGCCCGCCGACCGACTGCTCGA
CGAGGCCAACGAGGTGGCCGCGACGATCGCGGGGATGTCGCGCAACGCATCCCGGATGGCCAAGGAAGCGGTCGACCGTG
CCTTCGAGTCCACGCTGACCGAGGGCCTGCTCTACGAACGCCGGCTCTTCCACTCCACCTTCGCGACCGAGGATCAGAAG
GAAGGTATGGCGGCGTTCAGCGAGAAGCGCCCGGCCAACTTCACGCATCGCTAG

Upstream 100 bases:

>100_bases
CATCAGTCGGTCGACGAGGTCACCGCCGCGGACGTCGACGCCTATTTCGCACCCGCCGATCCCGAACTCACCTTTCCCTG
ACGACCCCTAAGGAGCAGCC

Downstream 100 bases:

>100_bases
ATTGCACGGGTGACCGACACCGCGCAGACCGTCGAGGAGACGCCTCCGCAGGAGACCCCACCGCCGAAACGGGCCTGGTG
GCTGCGGCATTACACGTTCT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MDYETILVTREGRVGTITLNRPKALNALNSQVMAEVTTAAAEFDSDEGIGAIVVTGSEKAFAAGADIKEMASLSFAEVFT
SDFFALWGKFAATRTPTIAAVAGYALGGGCELAMMCDILIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLIL
TGRTIGAEEAERSGLVSRVVPADRLLDEANEVAATIAGMSRNASRMAKEAVDRAFESTLTEGLLYERRLFHSTFATEDQK
EGMAAFSEKRPANFTHR

Sequences:

>Translated_257_residues
MDYETILVTREGRVGTITLNRPKALNALNSQVMAEVTTAAAEFDSDEGIGAIVVTGSEKAFAAGADIKEMASLSFAEVFT
SDFFALWGKFAATRTPTIAAVAGYALGGGCELAMMCDILIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLIL
TGRTIGAEEAERSGLVSRVVPADRLLDEANEVAATIAGMSRNASRMAKEAVDRAFESTLTEGLLYERRLFHSTFATEDQK
EGMAAFSEKRPANFTHR
>Mature_257_residues
MDYETILVTREGRVGTITLNRPKALNALNSQVMAEVTTAAAEFDSDEGIGAIVVTGSEKAFAAGADIKEMASLSFAEVFT
SDFFALWGKFAATRTPTIAAVAGYALGGGCELAMMCDILIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLIL
TGRTIGAEEAERSGLVSRVVPADRLLDEANEVAATIAGMSRNASRMAKEAVDRAFESTLTEGLLYERRLFHSTFATEDQK
EGMAAFSEKRPANFTHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=53.2818532818533, Blast_Score=284, Evalue=6e-77,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=38.2113821138211, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI68989263, Length=223, Percent_Identity=33.1838565022422, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI20127408, Length=203, Percent_Identity=33.9901477832512, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=27.6, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=33.6065573770492, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI70995211, Length=264, Percent_Identity=30.6818181818182, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=30.8510638297872, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=30.8510638297872, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=28.6363636363636, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=28.6363636363636, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=28.6363636363636, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=241, Percent_Identity=26.1410788381743, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI213417737, Length=241, Percent_Identity=26.1410788381743, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=37.037037037037, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI25453481, Length=188, Percent_Identity=27.6595744680851, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI51538573, Length=188, Percent_Identity=27.6595744680851, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI4757966, Length=188, Percent_Identity=27.6595744680851, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI51538581, Length=188, Percent_Identity=27.6595744680851, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI4757968, Length=188, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=188, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI62530384, Length=212, Percent_Identity=24.0566037735849, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694520, Length=223, Percent_Identity=25.1121076233184, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=43.6507936507937, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=37.890625, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=33.6842105263158, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788682, Length=194, Percent_Identity=35.5670103092784, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=30.5660377358491, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=53.2, Blast_Score=272, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=49.0118577075099, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=32.2727272727273, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=244, Percent_Identity=31.1475409836066, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=31.6666666666667, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=35, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=35, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=35, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=205, Percent_Identity=30.7317073170732, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=261, Percent_Identity=27.9693486590038, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=275, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=223, Percent_Identity=30.0448430493274, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=223, Percent_Identity=30.0448430493274, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=25.9842519685039, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=209, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=197, Percent_Identity=27.9187817258883, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=58.984375, Blast_Score=306, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=58.984375, Blast_Score=306, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24653139, Length=248, Percent_Identity=35.8870967741936, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21357171, Length=258, Percent_Identity=30.2325581395349, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=28.2945736434109, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650670, Length=262, Percent_Identity=30.9160305343511, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=29.9465240641711, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19920382, Length=248, Percent_Identity=30.6451612903226, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28571729, Length=191, Percent_Identity=29.8429319371728, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571730, Length=191, Percent_Identity=29.8429319371728, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921018, Length=196, Percent_Identity=27.0408163265306, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=32.7586206896552, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24583077, Length=172, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24583079, Length=172, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24583165, Length=174, Percent_Identity=27.0114942528736, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27341; Mature: 27341

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYETILVTREGRVGTITLNRPKALNALNSQVMAEVTTAAAEFDSDEGIGAIVVTGSEKA
CCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADIKEMASLSFAEVFTSDFFALWGKFAATRTPTIAAVAGYALGGGCELAMMCDILI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIGAEEAERSGLVSRVV
EECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHCHHHHHC
PADRLLDEANEVAATIAGMSRNASRMAKEAVDRAFESTLTEGLLYERRLFHSTFATEDQK
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EGMAAFSEKRPANFTHR
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDYETILVTREGRVGTITLNRPKALNALNSQVMAEVTTAAAEFDSDEGIGAIVVTGSEKA
CCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADIKEMASLSFAEVFTSDFFALWGKFAATRTPTIAAVAGYALGGGCELAMMCDILI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIGAEEAERSGLVSRVV
EECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHCHHHHHC
PADRLLDEANEVAATIAGMSRNASRMAKEAVDRAFESTLTEGLLYERRLFHSTFATEDQK
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EGMAAFSEKRPANFTHR
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]