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The map label for this gene is tcmP [H]

Identifier: 119870165

GI number: 119870165

Start: 4365374

End: 4366210

Strand: Direct

Name: tcmP [H]

Synonym: Mkms_4135

Alternate gene names: 119870165

Gene position: 4365374-4366210 (Clockwise)

Preceding gene: 119870164

Following gene: 119870167

Centisome position: 76.09

GC content: 69.3

Gene sequence:

>837_bases
GTGACGAACACACCGCCGAAGGTGGACGGCAGCGCGCTGACCGGGGTCTCGGAGACGGCGTTGCTCACCCTGCAGGTCCG
GGCCAACGAGGCCCGCCGCCGGGACGGGCTCATCGACGACCCGATGGCGATCCGGTTGGTCGACTCGATCGATTTCGACT
TCGGGAAGTTCGGCCCGAGCCGCCGCCAGGACATGGCGTTGCGGGCCAAGGGGTTCGACGAGTACACGCGCCGCTATCTC
CGCACGCATCCGAGGGCGACCGTCGTCGCGCTCGCCGAGGGACTGCAGACCAGCTTCCACCGGCTCGACGCCTCCGGTGT
GGGCCACGAGTTCCGGTGGCTGACCGTCGACCTCCCGCCCATCGTCGAGTTGCGCACCAAGCTCCTGCCGCCGTCGGAGC
GGGTCCGGGTGTGCGCGCAGTCCGCCCTGGACTTCACCTGGATGGACGAGGTCGACGACTCCGAGGGCGTGTTCATCACC
GCCGAGGGACTGCTGATGTACCTGCAGCCCGACGAGGCCATGTCGCTCATCCGGGCGTGCGCGCAGCGGTTCCCCGGCGC
TCAGATGATGTTCGACCTGCCGCCGTCGTGGTTCGCCTGGTGGGCGCGCCAGGGTATGCGAACGTCGTTGCGCTACAAGG
TGCCTCCGATGCCGTTCAGCCTCACCCCCGGTGAGGCTGCCGCGCTGGTGGACACCGTGCCCGGCATCCGCGCCGTACAC
GACGTGCCGCTGCCGGAGGGTCGCGGACGGGTGCTCAATGCGCTGCTGTGGACGGTTCAGCGGATTCCGGTGTTCGATGC
CGTGCGCCCGGTCTGGACCCTGCTCGAATTCGGCTGA

Upstream 100 bases:

>100_bases
CCATCGGCAAGACGATGGGTAAGAAGCGCTACAAGTCCTCGACCACCACCCGCAACCTGCGCACCCTGGACAAGGTGCTG
CGTTCGGTAGATTCGGCGAG

Downstream 100 bases:

>100_bases
CCGACGGCGGAGAGCCTCAGTCGAACGCGGACTCCACGTACCGCAGCGCCGCGGACTTCTCGACGCCCAGCGCCCGCGCC
GTCTCGACGTAGTTCTGCGC

Product: O-methyltransferase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTNTPPKVDGSALTGVSETALLTLQVRANEARRRDGLIDDPMAIRLVDSIDFDFGKFGPSRRQDMALRAKGFDEYTRRYL
RTHPRATVVALAEGLQTSFHRLDASGVGHEFRWLTVDLPPIVELRTKLLPPSERVRVCAQSALDFTWMDEVDDSEGVFIT
AEGLLMYLQPDEAMSLIRACAQRFPGAQMMFDLPPSWFAWWARQGMRTSLRYKVPPMPFSLTPGEAAALVDTVPGIRAVH
DVPLPEGRGRVLNALLWTVQRIPVFDAVRPVWTLLEFG

Sequences:

>Translated_278_residues
MTNTPPKVDGSALTGVSETALLTLQVRANEARRRDGLIDDPMAIRLVDSIDFDFGKFGPSRRQDMALRAKGFDEYTRRYL
RTHPRATVVALAEGLQTSFHRLDASGVGHEFRWLTVDLPPIVELRTKLLPPSERVRVCAQSALDFTWMDEVDDSEGVFIT
AEGLLMYLQPDEAMSLIRACAQRFPGAQMMFDLPPSWFAWWARQGMRTSLRYKVPPMPFSLTPGEAAALVDTVPGIRAVH
DVPLPEGRGRVLNALLWTVQRIPVFDAVRPVWTLLEFG
>Mature_277_residues
TNTPPKVDGSALTGVSETALLTLQVRANEARRRDGLIDDPMAIRLVDSIDFDFGKFGPSRRQDMALRAKGFDEYTRRYLR
THPRATVVALAEGLQTSFHRLDASGVGHEFRWLTVDLPPIVELRTKLLPPSERVRVCAQSALDFTWMDEVDDSEGVFITA
EGLLMYLQPDEAMSLIRACAQRFPGAQMMFDLPPSWFAWWARQGMRTSLRYKVPPMPFSLTPGEAAALVDTVPGIRAVHD
VPLPEGRGRVLNALLWTVQRIPVFDAVRPVWTLLEFG

Specific function: O-methyltransferase that catalyzes the methylation of the C-9 carboxy group of tetracenomycin E (TCM E) to yield TCM A2. Catalyzes as well the following side reactions:methylation of 8- O-methyl-TCM D3 to 9-carboxymethyl-8-O-methyl-TCM D3; and of TCM B3 t

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007213
- InterPro:   IPR016874 [H]

Pfam domain/function: PF04072 LCM [H]

EC number: NA

Molecular weight: Translated: 31221; Mature: 31090

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTPPKVDGSALTGVSETALLTLQVRANEARRRDGLIDDPMAIRLVDSIDFDFGKFGPS
CCCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHCCCCCCCCEEEEEHHCCCCHHCCCCC
RRQDMALRAKGFDEYTRRYLRTHPRATVVALAEGLQTSFHRLDASGVGHEFRWLTVDLPP
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
IVELRTKLLPPSERVRVCAQSALDFTWMDEVDDSEGVFITAEGLLMYLQPDEAMSLIRAC
HHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCEEEEECCHHHHHHHHHH
AQRFPGAQMMFDLPPSWFAWWARQGMRTSLRYKVPPMPFSLTPGEAAALVDTVPGIRAVH
HHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCEECCCCHHHHHHCCCCCHHHC
DVPLPEGRGRVLNALLWTVQRIPVFDAVRPVWTLLEFG
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNTPPKVDGSALTGVSETALLTLQVRANEARRRDGLIDDPMAIRLVDSIDFDFGKFGPS
CCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHCCCCCCCCEEEEEHHCCCCHHCCCCC
RRQDMALRAKGFDEYTRRYLRTHPRATVVALAEGLQTSFHRLDASGVGHEFRWLTVDLPP
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
IVELRTKLLPPSERVRVCAQSALDFTWMDEVDDSEGVFITAEGLLMYLQPDEAMSLIRAC
HHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCEEEEECCHHHHHHHHHH
AQRFPGAQMMFDLPPSWFAWWARQGMRTSLRYKVPPMPFSLTPGEAAALVDTVPGIRAVH
HHHCCCCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCEECCCCHHHHHHCCCCCHHHC
DVPLPEGRGRVLNALLWTVQRIPVFDAVRPVWTLLEFG
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]