Click here to switch to the map view.

The map label for this gene is nikA [C]

Identifier: 119870035

GI number: 119870035

Start: 4222327

End: 4223994

Strand: Direct

Name: nikA [C]

Synonym: Mkms_4005

Alternate gene names: 119870035

Gene position: 4222327-4223994 (Clockwise)

Preceding gene: 119870034

Following gene: 119870036

Centisome position: 73.6

GC content: 67.45

Gene sequence:

>1668_bases
ATGACGTTGCGACGCCTGATCTCGGCCGCCCTCGTTGCCACCCTCACCCTCGCCGCGTGTTCCAGCGGTGACGAGGAGAC
CCCGTCGGCCGGCGGTAGCGCGGAGGTGGGCGCCACCAACGACGTCAATCCTCAGGACGTGTCCAACCTGCGCCAGGGCG
GCAACCTGCGCTTGGCGCTGACCGCATTCCCGGCGAACTTCAACAGCCTGCACATCGACGGCAACGTCGCCGATGCGGCC
GGGATGCTCAGGGCCACGATGCCGCGTGCGTTCCGGATCGCCGCGGACGGTTCGGCGACGCTGAACACCGACTACTTCAC
CGGCGCCGAGATCACCGGCACCGACCCGCAGGTCGTCACCTGGACGATCAACCCGAAGGCGGTGTGGAGCGACGGCGCCC
CGATCACCTGGGAGGACATCGCCGCCCAGGTGAACGCCACCAGCGGTAAGCAGGAGGGGTTCGCCTTCGCCAGCCCCAAC
GGCTCCGACCGCGTCGCGTCGGTGACCCGCGGGGCCGACGACCGGCAGGCCGTCATGACCTTCGCCAAGCCCTACGCGGA
ATGGCGCGGCATGCTCTCGGGCAACACCATGCTGCTGCCCAAGAGCATGACCGCGACGCCCGAGGCGTTCAACCGCGGCC
AACTCGACGCGCCGGGCCCGTCGGCGGGTCCGTTCATCGTGTCGAACCTGGACCGTACGGCCCAGCGGATCACGCTGACC
CGCAACCCGAAATGGTGGGGTGAGCCGCCACTTCTGGACAGCATCACCTACTCGGTGCTCGACGACGCCGCCCGAATCCC
CGCGCTGCAGAACAACGCGCTGGACGCCACCGGTCTGGCGACCCTCGACGAGTTGACGATCGCGCGGCGCACCAACGGTG
TGGCGATCCGGCGGGCGCCGGGCAACAGCTGGTACCACTTCACGTTCAACGGGGCACCGGGGTCGATCCTGGCCGACAAG
GCGCTGCGGCAGGCGATCGCGAAAGGCATTGACCGCCAGACCATTGCGGCGGTCACCCAGCGCGGCCTCGCCGACGACCC
GGTGCCGCTCAACAACCACATCTTCGTCGCGGGCCAGCAGGGCTACCAGGACAACAGCGGTGTGGTGGCGTTCGACCCCG
AGAAGGCCAAACAGGAACTCGATGCGCTCGGATGGCGGCTCAACGGGCAGTTCCGGGAGAAGGACGGCCGCCAGCTGGTG
ATCCGCGACGTGCTGTTCGACGCGCTGAGCACCCGTCAGTTCGGCCAGATCGCGCAGAACAACCTCGCCCAGATCGGTGT
GAAACTCGAACTGGACGCCAAGGGTGCGGCCGGCTTCTTCACCGACTACATCAACACCGGCGACTTCGACATCGCGCAGT
TCTCGTGGGTGGGTGACGCGTTCCCGCTCTCGGGTCTGACGCAGATCTACGCCTCCAACGGGGAGAGCAACTTCGGCAAG
ATCGGCAGCCCGCAGATCGACGCGAAGATCGAGGAGGCGCTGGAGGAACTGGATCCGGCGAAGGCTCAGCAGAAGGCCAA
CGAGGTCGACAAACTGCTGTGGGACGAGGTGTTCAGCCTGCCGTTGACGCAGTCGCCGGGCAACGTGGCGGTGCGTGCCA
ACCTCGCCAACTTCGGGGCGTTCGGCCTCGCGGACGCCGACTACTCGAAGATCGGCTTCGTGAAGTAG

Upstream 100 bases:

>100_bases
ACGGGGACCGGGTCTTCACCGCGCCGCAACACGACTACACGCGCCGCCTGTTGGCGGCGGTACCCCAGCCGAGCGTCCCG
CAACGTTAGAGTCCATATGC

Downstream 100 bases:

>100_bases
CCGGGTTCGGGTCCCGCTGATCCAAACGGTGCTGCCCGGCAGGCGGCCTCGGTAGAACCGAGGACATGACGACTGTGGAG
AACGCCAGGGCCCTGCCCGG

Product: extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTLRRLISAALVATLTLAACSSGDEETPSAGGSAEVGATNDVNPQDVSNLRQGGNLRLALTAFPANFNSLHIDGNVADAA
GMLRATMPRAFRIAADGSATLNTDYFTGAEITGTDPQVVTWTINPKAVWSDGAPITWEDIAAQVNATSGKQEGFAFASPN
GSDRVASVTRGADDRQAVMTFAKPYAEWRGMLSGNTMLLPKSMTATPEAFNRGQLDAPGPSAGPFIVSNLDRTAQRITLT
RNPKWWGEPPLLDSITYSVLDDAARIPALQNNALDATGLATLDELTIARRTNGVAIRRAPGNSWYHFTFNGAPGSILADK
ALRQAIAKGIDRQTIAAVTQRGLADDPVPLNNHIFVAGQQGYQDNSGVVAFDPEKAKQELDALGWRLNGQFREKDGRQLV
IRDVLFDALSTRQFGQIAQNNLAQIGVKLELDAKGAAGFFTDYINTGDFDIAQFSWVGDAFPLSGLTQIYASNGESNFGK
IGSPQIDAKIEEALEELDPAKAQQKANEVDKLLWDEVFSLPLTQSPGNVAVRANLANFGAFGLADADYSKIGFVK

Sequences:

>Translated_555_residues
MTLRRLISAALVATLTLAACSSGDEETPSAGGSAEVGATNDVNPQDVSNLRQGGNLRLALTAFPANFNSLHIDGNVADAA
GMLRATMPRAFRIAADGSATLNTDYFTGAEITGTDPQVVTWTINPKAVWSDGAPITWEDIAAQVNATSGKQEGFAFASPN
GSDRVASVTRGADDRQAVMTFAKPYAEWRGMLSGNTMLLPKSMTATPEAFNRGQLDAPGPSAGPFIVSNLDRTAQRITLT
RNPKWWGEPPLLDSITYSVLDDAARIPALQNNALDATGLATLDELTIARRTNGVAIRRAPGNSWYHFTFNGAPGSILADK
ALRQAIAKGIDRQTIAAVTQRGLADDPVPLNNHIFVAGQQGYQDNSGVVAFDPEKAKQELDALGWRLNGQFREKDGRQLV
IRDVLFDALSTRQFGQIAQNNLAQIGVKLELDAKGAAGFFTDYINTGDFDIAQFSWVGDAFPLSGLTQIYASNGESNFGK
IGSPQIDAKIEEALEELDPAKAQQKANEVDKLLWDEVFSLPLTQSPGNVAVRANLANFGAFGLADADYSKIGFVK
>Mature_554_residues
TLRRLISAALVATLTLAACSSGDEETPSAGGSAEVGATNDVNPQDVSNLRQGGNLRLALTAFPANFNSLHIDGNVADAAG
MLRATMPRAFRIAADGSATLNTDYFTGAEITGTDPQVVTWTINPKAVWSDGAPITWEDIAAQVNATSGKQEGFAFASPNG
SDRVASVTRGADDRQAVMTFAKPYAEWRGMLSGNTMLLPKSMTATPEAFNRGQLDAPGPSAGPFIVSNLDRTAQRITLTR
NPKWWGEPPLLDSITYSVLDDAARIPALQNNALDATGLATLDELTIARRTNGVAIRRAPGNSWYHFTFNGAPGSILADKA
LRQAIAKGIDRQTIAAVTQRGLADDPVPLNNHIFVAGQQGYQDNSGVVAFDPEKAKQELDALGWRLNGQFREKDGRQLVI
RDVLFDALSTRQFGQIAQNNLAQIGVKLELDAKGAAGFFTDYINTGDFDIAQFSWVGDAFPLSGLTQIYASNGESNFGKI
GSPQIDAKIEEALEELDPAKAQQKANEVDKLLWDEVFSLPLTQSPGNVAVRANLANFGAFGLADADYSKIGFVK

Specific function: Involved In A Nickel Transport System, Probably Represents The Nickel Binder. [C]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59281; Mature: 59150

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRRLISAALVATLTLAACSSGDEETPSAGGSAEVGATNDVNPQDVSNLRQGGNLRLAL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEE
TAFPANFNSLHIDGNVADAAGMLRATMPRAFRIAADGSATLNTDYFTGAEITGTDPQVVT
EECCCCCCEEEECCCHHHHHHHHHHHCCCEEEEECCCCCEECCCEECCCEEECCCCEEEE
WTINPKAVWSDGAPITWEDIAAQVNATSGKQEGFAFASPNGSDRVASVTRGADDRQAVMT
EEECCCEEECCCCCCCHHHHHHEECCCCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHH
FAKPYAEWRGMLSGNTMLLPKSMTATPEAFNRGQLDAPGPSAGPFIVSNLDRTAQRITLT
HHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEEECHHHCCCEEEEE
RNPKWWGEPPLLDSITYSVLDDAARIPALQNNALDATGLATLDELTIARRTNGVAIRRAP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GNSWYHFTFNGAPGSILADKALRQAIAKGIDRQTIAAVTQRGLADDPVPLNNHIFVAGQQ
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECC
GYQDNSGVVAFDPEKAKQELDALGWRLNGQFREKDGRQLVIRDVLFDALSTRQFGQIAQN
CCCCCCCEEEECHHHHHHHHHHHCEEECCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHC
NLAQIGVKLELDAKGAAGFFTDYINTGDFDIAQFSWVGDAFPLSGLTQIYASNGESNFGK
CHHHEEEEEEECCCCCCCHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCC
IGSPQIDAKIEEALEELDPAKAQQKANEVDKLLWDEVFSLPLTQSPGNVAVRANLANFGA
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
FGLADADYSKIGFVK
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLRRLISAALVATLTLAACSSGDEETPSAGGSAEVGATNDVNPQDVSNLRQGGNLRLAL
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEE
TAFPANFNSLHIDGNVADAAGMLRATMPRAFRIAADGSATLNTDYFTGAEITGTDPQVVT
EECCCCCCEEEECCCHHHHHHHHHHHCCCEEEEECCCCCEECCCEECCCEEECCCCEEEE
WTINPKAVWSDGAPITWEDIAAQVNATSGKQEGFAFASPNGSDRVASVTRGADDRQAVMT
EEECCCEEECCCCCCCHHHHHHEECCCCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHH
FAKPYAEWRGMLSGNTMLLPKSMTATPEAFNRGQLDAPGPSAGPFIVSNLDRTAQRITLT
HHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEEECHHHCCCEEEEE
RNPKWWGEPPLLDSITYSVLDDAARIPALQNNALDATGLATLDELTIARRTNGVAIRRAP
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GNSWYHFTFNGAPGSILADKALRQAIAKGIDRQTIAAVTQRGLADDPVPLNNHIFVAGQQ
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECC
GYQDNSGVVAFDPEKAKQELDALGWRLNGQFREKDGRQLVIRDVLFDALSTRQFGQIAQN
CCCCCCCEEEECHHHHHHHHHHHCEEECCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHC
NLAQIGVKLELDAKGAAGFFTDYINTGDFDIAQFSWVGDAFPLSGLTQIYASNGESNFGK
CHHHEEEEEEECCCCCCCHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCC
IGSPQIDAKIEEALEELDPAKAQQKANEVDKLLWDEVFSLPLTQSPGNVAVRANLANFGA
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
FGLADADYSKIGFVK
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]