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The map label for this gene is shiA [H]

Identifier: 119869936

GI number: 119869936

Start: 4113194

End: 4114492

Strand: Direct

Name: shiA [H]

Synonym: Mkms_3906

Alternate gene names: 119869936

Gene position: 4113194-4114492 (Clockwise)

Preceding gene: 119869935

Following gene: 119869943

Centisome position: 71.69

GC content: 68.59

Gene sequence:

>1299_bases
ATGGCAGCGACCAGTGCCACCGCCACCGTCGGCACGTCGCCGCTGCGGGTCGCGTCGGCCAGTTTCATCGGAACGACCGT
CGAGTACTACGACTTCCTGATCTACGGCACCGCGGCCGCGCTGGTGTTCCCCAGACTGTTCTTTCCCGACGTGTCGCCGG
CCACCGGACTCCTGTTGTCCTTCGCGACGTTCGGGGTCGGCTTCGTCGCCCGCCCGTTGGGCGGCATCGTCTTCGGCCAC
TACGGCGACCGGGTGGGACGCAAGCGGATGCTGGTCTACTCGCTGCTCATCATGGGCACCGCGACGGTGCTCATGGGTGC
CCTGCCCACCTACGCGCAGATCGGTGTCGCGGCGCCGATCCTGTTGACGCTGTTGCGGTTGGCACAGGGCTTCGCCGTCG
GCGGTGAGTGGGGCGGCGCCACCCTGATGGCCGTCGAACACGCCACACCGGACCGCAAGGGGCTGTACGGGGCGTTCCCG
CAGATGGGCGCCCCCGCCGGTACGGCCACCGCGACGTTGGCGTTCTACGCCGTCTCGCTGCTGCCCGACGAGCAGTTCCT
GTCCTGGGGTTGGCGGATCCCGTTCCTCGCCAGCGCCGTGTTGATCGTCATCGGCCTGGTCATCAGGCTCTCGCTGGCGG
AGAGTCCCGAATTCGCGGCGGTGCAGGCCCGTGCCGCGGTCCTGCGGTTGCCGATCGCCGTGGCGTTCAGAAGACACTGG
CGGCAGATCCTGCTCGTCGCCGGGGCGTATCTGTCGCAGGGGGTGTTCGCCTACATCTGCGTGGCGTACCTGGTGTCCTA
CGCCACCACCGTGGCCGGGATCGACCGTGCCCAGGCACTTCTCGGTGTCTGTGTGGCCGCCGTGGTCGCGGTCGTGATGT
ATCCGGTGTTCGGCCGGGTGTCCGACGCCGTCGGCCGCAAACCGGTCTTCCTGGCCGGCGTGATCGCGATGGGCATGTCC
GTGCTGCCGGTCTTCGCGCTGATCAACACCGGTAGGCCGGGGCTGTTCCTGCTCGCGCTCGTGCTGGTGTTCGGGTTGGC
GATGGCACCGGCGGCCGGCGTGACCGGATCGCTGTTCAGCCTGGCATTCGACGCCGACGTGCGCTACAGCGGTGTCTCGC
TGGGCTATACGCTCTCGGCGGTGGTCGGATCGGCATTCGCACCGACCATCGCCACGGCCCTGTACGCGGCGACCGAATCC
AGCGATTCGATCGCCGCCTACATGATCGCGGTGTCGGTGATCTCGGCGGTGTCGGTCAGCCTGATGCCTGGGCCCTGGCG
GCAGCCCCGGGTCAGGTGA

Upstream 100 bases:

>100_bases
GACCATGCGCGAAACCGCCACCCGCCCACTACGGATGCGGGTCTGACCGACCCGCGAAAAGCCTTGCCGGGCCGTGGATC
GGCCGATAGTGTCCGAATGC

Downstream 100 bases:

>100_bases
CACCGACTCAGGTGTCGATGTCGGCCAGCGCCTCGGAGGTCTGCAGGTCGGCGTACGCCGCCGAATACCGTTGCGCCAGT
GCGATCGCGATATCCGGACG

Product: major facilitator superfamily transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MAATSATATVGTSPLRVASASFIGTTVEYYDFLIYGTAAALVFPRLFFPDVSPATGLLLSFATFGVGFVARPLGGIVFGH
YGDRVGRKRMLVYSLLIMGTATVLMGALPTYAQIGVAAPILLTLLRLAQGFAVGGEWGGATLMAVEHATPDRKGLYGAFP
QMGAPAGTATATLAFYAVSLLPDEQFLSWGWRIPFLASAVLIVIGLVIRLSLAESPEFAAVQARAAVLRLPIAVAFRRHW
RQILLVAGAYLSQGVFAYICVAYLVSYATTVAGIDRAQALLGVCVAAVVAVVMYPVFGRVSDAVGRKPVFLAGVIAMGMS
VLPVFALINTGRPGLFLLALVLVFGLAMAPAAGVTGSLFSLAFDADVRYSGVSLGYTLSAVVGSAFAPTIATALYAATES
SDSIAAYMIAVSVISAVSVSLMPGPWRQPRVR

Sequences:

>Translated_432_residues
MAATSATATVGTSPLRVASASFIGTTVEYYDFLIYGTAAALVFPRLFFPDVSPATGLLLSFATFGVGFVARPLGGIVFGH
YGDRVGRKRMLVYSLLIMGTATVLMGALPTYAQIGVAAPILLTLLRLAQGFAVGGEWGGATLMAVEHATPDRKGLYGAFP
QMGAPAGTATATLAFYAVSLLPDEQFLSWGWRIPFLASAVLIVIGLVIRLSLAESPEFAAVQARAAVLRLPIAVAFRRHW
RQILLVAGAYLSQGVFAYICVAYLVSYATTVAGIDRAQALLGVCVAAVVAVVMYPVFGRVSDAVGRKPVFLAGVIAMGMS
VLPVFALINTGRPGLFLLALVLVFGLAMAPAAGVTGSLFSLAFDADVRYSGVSLGYTLSAVVGSAFAPTIATALYAATES
SDSIAAYMIAVSVISAVSVSLMPGPWRQPRVR
>Mature_431_residues
AATSATATVGTSPLRVASASFIGTTVEYYDFLIYGTAAALVFPRLFFPDVSPATGLLLSFATFGVGFVARPLGGIVFGHY
GDRVGRKRMLVYSLLIMGTATVLMGALPTYAQIGVAAPILLTLLRLAQGFAVGGEWGGATLMAVEHATPDRKGLYGAFPQ
MGAPAGTATATLAFYAVSLLPDEQFLSWGWRIPFLASAVLIVIGLVIRLSLAESPEFAAVQARAAVLRLPIAVAFRRHWR
QILLVAGAYLSQGVFAYICVAYLVSYATTVAGIDRAQALLGVCVAAVVAVVMYPVFGRVSDAVGRKPVFLAGVIAMGMSV
LPVFALINTGRPGLFLLALVLVFGLAMAPAAGVTGSLFSLAFDADVRYSGVSLGYTLSAVVGSAFAPTIATALYAATESS
DSIAAYMIAVSVISAVSVSLMPGPWRQPRVR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=417, Percent_Identity=41.9664268585132, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1789941, Length=384, Percent_Identity=43.4895833333333, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1790550, Length=432, Percent_Identity=31.9444444444444, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1788942, Length=378, Percent_Identity=32.2751322751323, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI87082231, Length=472, Percent_Identity=22.4576271186441, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082404, Length=348, Percent_Identity=27.2988505747126, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44999; Mature: 44867

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATSATATVGTSPLRVASASFIGTTVEYYDFLIYGTAAALVFPRLFFPDVSPATGLLLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FATFGVGFVARPLGGIVFGHYGDRVGRKRMLVYSLLIMGTATVLMGALPTYAQIGVAAPI
HHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLTLLRLAQGFAVGGEWGGATLMAVEHATPDRKGLYGAFPQMGAPAGTATATLAFYAVSL
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LPDEQFLSWGWRIPFLASAVLIVIGLVIRLSLAESPEFAAVQARAAVLRLPIAVAFRRHW
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RQILLVAGAYLSQGVFAYICVAYLVSYATTVAGIDRAQALLGVCVAAVVAVVMYPVFGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDAVGRKPVFLAGVIAMGMSVLPVFALINTGRPGLFLLALVLVFGLAMAPAAGVTGSLFS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
LAFDADVRYSGVSLGYTLSAVVGSAFAPTIATALYAATESSDSIAAYMIAVSVISAVSVS
HEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LMPGPWRQPRVR
CCCCCCCCCCCC
>Mature Secondary Structure 
AATSATATVGTSPLRVASASFIGTTVEYYDFLIYGTAAALVFPRLFFPDVSPATGLLLS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FATFGVGFVARPLGGIVFGHYGDRVGRKRMLVYSLLIMGTATVLMGALPTYAQIGVAAPI
HHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLTLLRLAQGFAVGGEWGGATLMAVEHATPDRKGLYGAFPQMGAPAGTATATLAFYAVSL
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LPDEQFLSWGWRIPFLASAVLIVIGLVIRLSLAESPEFAAVQARAAVLRLPIAVAFRRHW
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RQILLVAGAYLSQGVFAYICVAYLVSYATTVAGIDRAQALLGVCVAAVVAVVMYPVFGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDAVGRKPVFLAGVIAMGMSVLPVFALINTGRPGLFLLALVLVFGLAMAPAAGVTGSLFS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
LAFDADVRYSGVSLGYTLSAVVGSAFAPTIATALYAATESSDSIAAYMIAVSVISAVSVS
HEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LMPGPWRQPRVR
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]