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The map label for this gene is proP [H]

Identifier: 119869935

GI number: 119869935

Start: 4111829

End: 4113139

Strand: Direct

Name: proP [H]

Synonym: Mkms_3905

Alternate gene names: 119869935

Gene position: 4111829-4113139 (Clockwise)

Preceding gene: 119869934

Following gene: 119869936

Centisome position: 71.67

GC content: 66.67

Gene sequence:

>1311_bases
ATGGCCACCATCGACGGACACCGCCCGCGGGAGGCAGAACCGCTCGCGGTGCGGCGCGCGGTGCGGGGCGCCGCGATCGG
CAACACCGTCGAGTGGTTCGACTTCGCGATCTACGGCTTCCTCGCCACCTACATCGCGGAGAAGTTCTTCCCCACCGCCG
ATGACACCGCCGCACTGCTCAACACCTTCGCGATCTTCGCCGCCGCCTTCTTCATGCGGCCGCTCGGCGGGTTCTTCTTC
GGCCCCCTCGGGGACCGGATCGGGCGTCAGCGTGTGCTGGCCCTGGTCATCCTGCTGATGTCGGCGTCGACGTTCGCGAT
CGGCCTGCTGCCGAGCTACGACGCCATCGGTGTCGCCGCCCCGCTGCTGCTGCTCCTGCTGCGGTGTCTGCAGGGATTCT
CCGCCGGCGGCGAATACGGTAGCGGCGCCTGCTATCTCGCCGAATTCGCACCGGACCGCCACCGCGGGTTCGTCGTGAGC
TTCCTGGTGTGGTCGGTCGTGGTCGGATTCCTGCTCGGGTCGTTGACGGTCACCGCGCTCGAGGCGGTGCTCGACGAATC
GGCGATGGACTCCTACGGGTGGCGCATCCCGTTCCTCATCGCCGGCGTGCTGGGCGGCGTCGGACTCTACATCCGGTTGC
GGCTGGGTGACACACCGGATTTCGAGGAGCTCAAGGAATCCGGCGAGGTGTCCACCTCACCGCTCAAGGATGCGGTGAGA
ACCTCGTGGAAACCGATCCTGCAGATCGCCGGGCTCGTCATCATCCACAACGTCGGCTTCTACACGGTGTTCACCTACCT
GCCGAGCTACATGACCGAGACGCTCGACTTCACCAAAACCGATGCGTTCCTCTCGATCACCGTGGCCAGTGTGGTCGGAT
TGGTGCTCATCCTCCCGCTCGGCGCACTGTCGGACCGCCTCGGCCGCAAACCGTTGCTGATCGCGGGTGCGGTGGGCTTC
GCCGTCTTCGCCTATCCGCTGTTCGCACTCTTCAACACCGGATCGCTGCTCGGCGCGATCGCCGGACACGCCGGTCTGTC
GGCACTCGAGGCGCTGTTCGTCGCGGGCTCACTGGCCGCGGGCGCGGAGTTGTTCGCCACCCGGGTGCGCTCCAGCGGCT
ACTCGATCGGCTACAACACGTCGGTGGCGCTGTTCGGCGGTACCGCACCGTATGTCGCGACCTGGCTCACCGACCGCACC
GGAAACGACCTCGCACCGGCCTACTATCTGATCGTCGCCGCCGTCATCTCGTTGGCCACCATCCTGACCATGCGCGAAAC
CGCCACCCGCCCACTACGGATGCGGGTCTGA

Upstream 100 bases:

>100_bases
GATCTTCACCCCGTGTGAGAACACACGTTAGTTTCCGGACAAGATCGGGAATGCTCGGTCCCAGCAGTGCTGGGGTCGAC
GTCGCGATCGAGGAGCAGCC

Downstream 100 bases:

>100_bases
CCGACCCGCGAAAAGCCTTGCCGGGCCGTGGATCGGCCGATAGTGTCCGAATGCATGGCAGCGACCAGTGCCACCGCCAC
CGTCGGCACGTCGCCGCTGC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MATIDGHRPREAEPLAVRRAVRGAAIGNTVEWFDFAIYGFLATYIAEKFFPTADDTAALLNTFAIFAAAFFMRPLGGFFF
GPLGDRIGRQRVLALVILLMSASTFAIGLLPSYDAIGVAAPLLLLLLRCLQGFSAGGEYGSGACYLAEFAPDRHRGFVVS
FLVWSVVVGFLLGSLTVTALEAVLDESAMDSYGWRIPFLIAGVLGGVGLYIRLRLGDTPDFEELKESGEVSTSPLKDAVR
TSWKPILQIAGLVIIHNVGFYTVFTYLPSYMTETLDFTKTDAFLSITVASVVGLVLILPLGALSDRLGRKPLLIAGAVGF
AVFAYPLFALFNTGSLLGAIAGHAGLSALEALFVAGSLAAGAELFATRVRSSGYSIGYNTSVALFGGTAPYVATWLTDRT
GNDLAPAYYLIVAAVISLATILTMRETATRPLRMRV

Sequences:

>Translated_436_residues
MATIDGHRPREAEPLAVRRAVRGAAIGNTVEWFDFAIYGFLATYIAEKFFPTADDTAALLNTFAIFAAAFFMRPLGGFFF
GPLGDRIGRQRVLALVILLMSASTFAIGLLPSYDAIGVAAPLLLLLLRCLQGFSAGGEYGSGACYLAEFAPDRHRGFVVS
FLVWSVVVGFLLGSLTVTALEAVLDESAMDSYGWRIPFLIAGVLGGVGLYIRLRLGDTPDFEELKESGEVSTSPLKDAVR
TSWKPILQIAGLVIIHNVGFYTVFTYLPSYMTETLDFTKTDAFLSITVASVVGLVLILPLGALSDRLGRKPLLIAGAVGF
AVFAYPLFALFNTGSLLGAIAGHAGLSALEALFVAGSLAAGAELFATRVRSSGYSIGYNTSVALFGGTAPYVATWLTDRT
GNDLAPAYYLIVAAVISLATILTMRETATRPLRMRV
>Mature_435_residues
ATIDGHRPREAEPLAVRRAVRGAAIGNTVEWFDFAIYGFLATYIAEKFFPTADDTAALLNTFAIFAAAFFMRPLGGFFFG
PLGDRIGRQRVLALVILLMSASTFAIGLLPSYDAIGVAAPLLLLLLRCLQGFSAGGEYGSGACYLAEFAPDRHRGFVVSF
LVWSVVVGFLLGSLTVTALEAVLDESAMDSYGWRIPFLIAGVLGGVGLYIRLRLGDTPDFEELKESGEVSTSPLKDAVRT
SWKPILQIAGLVIIHNVGFYTVFTYLPSYMTETLDFTKTDAFLSITVASVVGLVLILPLGALSDRLGRKPLLIAGAVGFA
VFAYPLFALFNTGSLLGAIAGHAGLSALEALFVAGSLAAGAELFATRVRSSGYSIGYNTSVALFGGTAPYVATWLTDRTG
NDLAPAYYLIVAAVISLATILTMRETATRPLRMRV

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=41.2177985948478, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1788942, Length=412, Percent_Identity=36.1650485436893, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=32.183908045977, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789941, Length=444, Percent_Identity=33.5585585585586, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI87082404, Length=440, Percent_Identity=25.6818181818182, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082231, Length=240, Percent_Identity=28.75, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46510; Mature: 46378

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIDGHRPREAEPLAVRRAVRGAAIGNTVEWFDFAIYGFLATYIAEKFFPTADDTAALL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NTFAIFAAAFFMRPLGGFFFGPLGDRIGRQRVLALVILLMSASTFAIGLLPSYDAIGVAA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHH
PLLLLLLRCLQGFSAGGEYGSGACYLAEFAPDRHRGFVVSFLVWSVVVGFLLGSLTVTAL
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EAVLDESAMDSYGWRIPFLIAGVLGGVGLYIRLRLGDTPDFEELKESGEVSTSPLKDAVR
HHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHH
TSWKPILQIAGLVIIHNVGFYTVFTYLPSYMTETLDFTKTDAFLSITVASVVGLVLILPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GALSDRLGRKPLLIAGAVGFAVFAYPLFALFNTGSLLGAIAGHAGLSALEALFVAGSLAA
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAELFATRVRSSGYSIGYNTSVALFGGTAPYVATWLTDRTGNDLAPAYYLIVAAVISLAT
HHHHHHHHHHHCCCEECCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ILTMRETATRPLRMRV
HHHHHHHCCCCCEECC
>Mature Secondary Structure 
ATIDGHRPREAEPLAVRRAVRGAAIGNTVEWFDFAIYGFLATYIAEKFFPTADDTAALL
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NTFAIFAAAFFMRPLGGFFFGPLGDRIGRQRVLALVILLMSASTFAIGLLPSYDAIGVAA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHH
PLLLLLLRCLQGFSAGGEYGSGACYLAEFAPDRHRGFVVSFLVWSVVVGFLLGSLTVTAL
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EAVLDESAMDSYGWRIPFLIAGVLGGVGLYIRLRLGDTPDFEELKESGEVSTSPLKDAVR
HHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHH
TSWKPILQIAGLVIIHNVGFYTVFTYLPSYMTETLDFTKTDAFLSITVASVVGLVLILPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GALSDRLGRKPLLIAGAVGFAVFAYPLFALFNTGSLLGAIAGHAGLSALEALFVAGSLAA
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAELFATRVRSSGYSIGYNTSVALFGGTAPYVATWLTDRTGNDLAPAYYLIVAAVISLAT
HHHHHHHHHHHCCCEECCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ILTMRETATRPLRMRV
HHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]