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The map label for this gene is pfkB [H]

Identifier: 119869507

GI number: 119869507

Start: 3650333

End: 3651307

Strand: Direct

Name: pfkB [H]

Synonym: Mkms_3475

Alternate gene names: 119869507

Gene position: 3650333-3651307 (Clockwise)

Preceding gene: 119869506

Following gene: 119869515

Centisome position: 63.63

GC content: 68.72

Gene sequence:

>975_bases
ATGAGACGGTCCGGCGAACGCACAGGACACGGACCGGCCATCGTCACGCTGACGATGAACCCGGCGCTCGACATCACCAC
GAGCGCGCCGCAGGTCCGGCCGACCAGCAAGATCCGTTGTGTGGGAGCCCGTTACGACCCTGGAGGCGGTGGCATCAACG
TGGCCCGCACCGCTCACGTCCTCGGCGAGTCGGTGTCGGCGGTGTTCCCCGTGGGCGGGCCGACCGGCGACCTGGTCACA
GAACTGCTCGGCAAGGCCGATGTTCCGTTCCGTCGTATCGCGATCGGAGATCCGACGCGCGAGAGCTTCACCGTCAACGA
CGACAGCAGCGGCCAGCAGTACCGGTTCGTCCTTCCCGGCCCCTGTCTGACCGCACAGGAACAGGCGAAGTGTCTGGAGG
AACTGCGTTTCGAGGCCCGCGCGGCGCAGTTCGTGGTGGCAAGTGGCAGCCTGCCGCCGGGCGTTCCCTCGGACTTCTAC
CAGCGGGTCGCCGACATCTGCCGCGACGTCGGCGCACGGCTGATCCTCGACACCTCGGGTGACGCCCTCCAGAGCGTCCA
CTCGGGGGTCTTCCTGCTCAAAGCCAGTGTGCGCGAACTACGGGAGCGGGTCGGCCGCGATCTGCTCGACCGGTCCGATC
AGCTGACCGCCGCACAGGAACTCATCGCCGCCGGCGTCGCCGAGATCATCGTGGTGTCCCTGGGTTCGGAGGGCGCGCTG
CTGGCCACCTCGCAGGGCGGTCACCATTTCCCGCCGGTGCCCGTGGAGGCCGTCAGTGGTGTCGGCGCGGGGGACGCGAT
GGTGGCGGCGATCGCCGTCGGCCTGACTCGCGGGTGGTCGCCGACCAAGTCCGTGCAGTTCGGGGTCGCCACCGGAGCTG
CCATGTTGTTGACGCCGGGGACCGAGGTGTGCGCACGAGCCGACGTGGAACGGCTCTTCGAAGCGACGCCGGAGCCCGAA
GAGATTGTCGGCTAA

Upstream 100 bases:

>100_bases
GCTTCGCAAGCACCTGGCTGGGTTCGGTGAGCTCGGCGGTGGCGCAGTCCGCGGACACCCCGGTGCTCGTGGTGCGCGAC
GCATCGAGGGGTCAGGGCCG

Downstream 100 bases:

>100_bases
CGGATCCGGCCGCCCGCGCCCGGCCGCGCGGGTGCCCGATACAGCGCGGCGCCCGCGGTGATCCCGGCCCCCGCGGCGAC
GATCAGGATCAGGCCCTCCG

Product: 6-phosphofructokinase

Products: ADP; D-fructose 1,6-bisphosphate

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRRSGERTGHGPAIVTLTMNPALDITTSAPQVRPTSKIRCVGARYDPGGGGINVARTAHVLGESVSAVFPVGGPTGDLVT
ELLGKADVPFRRIAIGDPTRESFTVNDDSSGQQYRFVLPGPCLTAQEQAKCLEELRFEARAAQFVVASGSLPPGVPSDFY
QRVADICRDVGARLILDTSGDALQSVHSGVFLLKASVRELRERVGRDLLDRSDQLTAAQELIAAGVAEIIVVSLGSEGAL
LATSQGGHHFPPVPVEAVSGVGAGDAMVAAIAVGLTRGWSPTKSVQFGVATGAAMLLTPGTEVCARADVERLFEATPEPE
EIVG

Sequences:

>Translated_324_residues
MRRSGERTGHGPAIVTLTMNPALDITTSAPQVRPTSKIRCVGARYDPGGGGINVARTAHVLGESVSAVFPVGGPTGDLVT
ELLGKADVPFRRIAIGDPTRESFTVNDDSSGQQYRFVLPGPCLTAQEQAKCLEELRFEARAAQFVVASGSLPPGVPSDFY
QRVADICRDVGARLILDTSGDALQSVHSGVFLLKASVRELRERVGRDLLDRSDQLTAAQELIAAGVAEIIVVSLGSEGAL
LATSQGGHHFPPVPVEAVSGVGAGDAMVAAIAVGLTRGWSPTKSVQFGVATGAAMLLTPGTEVCARADVERLFEATPEPE
EIVG
>Mature_324_residues
MRRSGERTGHGPAIVTLTMNPALDITTSAPQVRPTSKIRCVGARYDPGGGGINVARTAHVLGESVSAVFPVGGPTGDLVT
ELLGKADVPFRRIAIGDPTRESFTVNDDSSGQQYRFVLPGPCLTAQEQAKCLEELRFEARAAQFVVASGSLPPGVPSDFY
QRVADICRDVGARLILDTSGDALQSVHSGVFLLKASVRELRERVGRDLLDRSDQLTAAQELIAAGVAEIIVVSLGSEGAL
LATSQGGHHFPPVPVEAVSGVGAGDAMVAAIAVGLTRGWSPTKSVQFGVATGAAMLLTPGTEVCARADVERLFEATPEPE
EIVG

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=302, Percent_Identity=40.3973509933775, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1788493, Length=265, Percent_Identity=29.0566037735849, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.11

Molecular weight: Translated: 33790; Mature: 33790

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSGERTGHGPAIVTLTMNPALDITTSAPQVRPTSKIRCVGARYDPGGGGINVARTAHV
CCCCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCEEEEECEECCCCCCCCHHHHHHH
LGESVSAVFPVGGPTGDLVTELLGKADVPFRRIAIGDPTRESFTVNDDSSGQQYRFVLPG
HHCCCCEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCCCEEEEEECC
PCLTAQEQAKCLEELRFEARAAQFVVASGSLPPGVPSDFYQRVADICRDVGARLILDTSG
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCC
DALQSVHSGVFLLKASVRELRERVGRDLLDRSDQLTAAQELIAAGVAEIIVVSLGSEGAL
HHHHHHHCCHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCEE
LATSQGGHHFPPVPVEAVSGVGAGDAMVAAIAVGLTRGWSPTKSVQFGVATGAAMLLTPG
EEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCC
TEVCARADVERLFEATPEPEEIVG
HHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure
MRRSGERTGHGPAIVTLTMNPALDITTSAPQVRPTSKIRCVGARYDPGGGGINVARTAHV
CCCCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCEEEEECEECCCCCCCCHHHHHHH
LGESVSAVFPVGGPTGDLVTELLGKADVPFRRIAIGDPTRESFTVNDDSSGQQYRFVLPG
HHCCCCEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCCCEEEEEECC
PCLTAQEQAKCLEELRFEARAAQFVVASGSLPPGVPSDFYQRVADICRDVGARLILDTSG
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCC
DALQSVHSGVFLLKASVRELRERVGRDLLDRSDQLTAAQELIAAGVAEIIVVSLGSEGAL
HHHHHHHCCHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCCEE
LATSQGGHHFPPVPVEAVSGVGAGDAMVAAIAVGLTRGWSPTKSVQFGVATGAAMLLTPG
EEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCC
TEVCARADVERLFEATPEPEEIVG
HHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose 6-phosphate

Specific reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]