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The map label for this gene is icaA [H]

Identifier: 119869448

GI number: 119869448

Start: 3582609

End: 3584978

Strand: Direct

Name: icaA [H]

Synonym: Mkms_3416

Alternate gene names: 119869448

Gene position: 3582609-3584978 (Clockwise)

Preceding gene: 119869447

Following gene: 119869450

Centisome position: 62.44

GC content: 65.49

Gene sequence:

>2370_bases
GTGTTCAGCCCCTTCAGAAGACGCCCGGACGCCATCCGCGGCACCGACCCTCTGACCGCCGCACCCGGTCCGGTGTTCTT
CGACCGGACGGGAAAACGTCTGTACTACTTCGCCGCCGGTGTCATCGTGCTCGCGGTGATCGTGGCAGTGCTGCCGGTCC
GGGTGACCCCCATGGCCTTCGATCCGCTGTGGACCGTGCCGACCAACAGTGATTCGGGCTTCCCGCGTCGCGTGCTGTCC
ACCGCCGACGACCGGCGCTTTCCCGTTCTCGGCAGCGAGGATGACGAGGTCCTCAGCAGGGTGGTCCGGGTCGACCGGCG
GGGTGATCAGTGGAATCTCGTCGACCCGTTCACCAATCAGCTGTACCGGGTCGCCGACGATGACGACAAGGATCGGATTC
GCGACAGTGCCTACGCGATAGACCATTACGGCCGTCCGCCGGACCGCCACCTGATGCTGACATTCGACGACGGACCCGAC
GTCGAGTTCACCACCCAGGTGCTCGACATCCTGTCCCGCGAGAAGATTCCCGCGACCTTCTTCGTCCTCGGCTCTCAGGT
CGTCCTGCATCCGGATGTCTTCCGGCGGATGATCCGCGAGGGCCATATGGCCGGCAACCACACGATGTTCCATGTCGACT
TCGACGAGCACACCGATTTCCGCAACCGGCAGGAGATCATCGCCACCGACCGCGTCATGCGCGCCACCGCGGCCTATGCC
AGCAGACTCTTCCGGATCCCGCGAGGCGACCCCGACAACAACACGCTCGCGCTGCTGCAGTCACAGCAGCTCGGATACCT
CCAGGTCGACCAGGACATCGACACCCTCGACTGGAAGGTGTCGCCGGAGAACGAGGTGGCGGTCCCACAACTCGACGGGC
GTGGACACGTCGTCCTCCTGCACGACGGTGGCGGCGACCGGTCAGCGACCGTCCGCATGCTCGAGCAACTGATCGCCGAG
GCCAAGAGCAAGGGCTACACCTTCTCGACGCTCGCACCGCTGCTGTCTGAGCAGGAGCTGCCCGTGAGCAACGCCGAACA
GTATCCGGCCGACGCCGCGACGTACCACACGCTGCGGCTGATGGAGAAGGCGCCGGGAGCAGTGCTCGGGTTCCTGTTCT
GGCTGGGCATGGGCTCGCTGACGGTGATGTCGTTGCTGTACCTGATACTCGCGCTCGTCTGTCAATACCGGCAGAACCGG
TTGCGCTGGAACGACATCGGCGATGACCAGCTGCCGATGGTGAGCGTGGTGTTGGCCGCCTTCAACGAGGAGAAGGTGAT
CGCGAGAACGATTGCCGAACTCCGCCGCAGCGACTACCCGCGGTCGAGGTTCGAGGTGGTCGCGGTCAACGACGGGTCCA
CGGACGGCACCCTGCGGATCCTCACCGAACTGGCGCGCGACTGGCCCAAATTGCGCGTGGTCGACCAGGCGAACAGCGGA
AAATCGTCGGCGATCAACAACGGGATCAATCACGCGTCGGCGGTGTCGACCGTGATGGTCACGATGGACGCGGACACCCT
CTTCAGACCGGACACGATCCGCAACCTCGCCCGGCACTTCGCCCGCCACACCCACGGCAGACAGGTCGGCGCGGTGGCCG
GGCACATCAAGGTCGGCAATCGGCGCAATCTCCTGACCGCCTGGCAGAGCCTCGAGTACATCTCCGGAATATGCGTCACC
CGTATGGCGGAGCGCCTGCTCAACGCGATCTCGATCGTCCCCGGCGCGTGCTCGGCGTGGAGCCGCACGGCCCTCGAGGA
GATCGGCGGCTTCTGCGATGACACCATGGCCGAGGACTGTGACGCGACCCTGGCGCTGCAGCGACGCGGTTACCGGATCC
TGCAGGAGAACAACGCCATCGCCGACACCGAGGCGCCGGAAACCATCCGTGCCCTTGCCAAACAACGCAAACGGTGGACC
TACGGCAACATCCAGGCGCTGTGGAAGCACCGCGCCATGCTGTTCCGGCCGCGCTACGGGGCACTGGGTCTGGTCGCGTT
GCCCTACGCCGCGCTCTCGCTCATCGTGCCGCTGCTGTTCATGCCGCTGACGATCGTCGCGGCCGGGATGAGCCTCGCGG
CCGGCAACTGGCAGAGCATCGCGCTGTTCGCGGGATTCGTTGCGGCACTGCACATGATCATCTCGATCACCGCTGTCGCG
ATGGCCCGGGAACGCGCCTGGCATCTGCTCGTCGTCCCCGTCTACCGGATCATCTACGAGCCGCTGCGGGCCTATCTGCT
CTACGCCTCGGCCTATCGGGCCATCAAGGGCACCATCGTGGCCTGGGACAAGTTGGAACGCAGGAACACGGTGAGCGCCT
TCGTCGAACGTCACCGCCCGATGCCACCGATCCTCGGGGCTCAGCAGTAG

Upstream 100 bases:

>100_bases
CCGCGGTGACTCTCGCCGAGGGGGTCGCCCGCACGGTCGAGTGGTTGCGAGCGACGCTCGACTCCGACTGTGACCGGCAA
GAGGCAGGGTGAGAACGACC

Downstream 100 bases:

>100_bases
AGGTCCTCCCCCGGCGGCACCCCGAGCAGCGCGCTGATCCGCAGATAGGCGTCCACCCGGCTCTGCACCTGCGCCGGATT
GCCGCCGCCACACTCGAGAA

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MFSPFRRRPDAIRGTDPLTAAPGPVFFDRTGKRLYYFAAGVIVLAVIVAVLPVRVTPMAFDPLWTVPTNSDSGFPRRVLS
TADDRRFPVLGSEDDEVLSRVVRVDRRGDQWNLVDPFTNQLYRVADDDDKDRIRDSAYAIDHYGRPPDRHLMLTFDDGPD
VEFTTQVLDILSREKIPATFFVLGSQVVLHPDVFRRMIREGHMAGNHTMFHVDFDEHTDFRNRQEIIATDRVMRATAAYA
SRLFRIPRGDPDNNTLALLQSQQLGYLQVDQDIDTLDWKVSPENEVAVPQLDGRGHVVLLHDGGGDRSATVRMLEQLIAE
AKSKGYTFSTLAPLLSEQELPVSNAEQYPADAATYHTLRLMEKAPGAVLGFLFWLGMGSLTVMSLLYLILALVCQYRQNR
LRWNDIGDDQLPMVSVVLAAFNEEKVIARTIAELRRSDYPRSRFEVVAVNDGSTDGTLRILTELARDWPKLRVVDQANSG
KSSAINNGINHASAVSTVMVTMDADTLFRPDTIRNLARHFARHTHGRQVGAVAGHIKVGNRRNLLTAWQSLEYISGICVT
RMAERLLNAISIVPGACSAWSRTALEEIGGFCDDTMAEDCDATLALQRRGYRILQENNAIADTEAPETIRALAKQRKRWT
YGNIQALWKHRAMLFRPRYGALGLVALPYAALSLIVPLLFMPLTIVAAGMSLAAGNWQSIALFAGFVAALHMIISITAVA
MARERAWHLLVVPVYRIIYEPLRAYLLYASAYRAIKGTIVAWDKLERRNTVSAFVERHRPMPPILGAQQ

Sequences:

>Translated_789_residues
MFSPFRRRPDAIRGTDPLTAAPGPVFFDRTGKRLYYFAAGVIVLAVIVAVLPVRVTPMAFDPLWTVPTNSDSGFPRRVLS
TADDRRFPVLGSEDDEVLSRVVRVDRRGDQWNLVDPFTNQLYRVADDDDKDRIRDSAYAIDHYGRPPDRHLMLTFDDGPD
VEFTTQVLDILSREKIPATFFVLGSQVVLHPDVFRRMIREGHMAGNHTMFHVDFDEHTDFRNRQEIIATDRVMRATAAYA
SRLFRIPRGDPDNNTLALLQSQQLGYLQVDQDIDTLDWKVSPENEVAVPQLDGRGHVVLLHDGGGDRSATVRMLEQLIAE
AKSKGYTFSTLAPLLSEQELPVSNAEQYPADAATYHTLRLMEKAPGAVLGFLFWLGMGSLTVMSLLYLILALVCQYRQNR
LRWNDIGDDQLPMVSVVLAAFNEEKVIARTIAELRRSDYPRSRFEVVAVNDGSTDGTLRILTELARDWPKLRVVDQANSG
KSSAINNGINHASAVSTVMVTMDADTLFRPDTIRNLARHFARHTHGRQVGAVAGHIKVGNRRNLLTAWQSLEYISGICVT
RMAERLLNAISIVPGACSAWSRTALEEIGGFCDDTMAEDCDATLALQRRGYRILQENNAIADTEAPETIRALAKQRKRWT
YGNIQALWKHRAMLFRPRYGALGLVALPYAALSLIVPLLFMPLTIVAAGMSLAAGNWQSIALFAGFVAALHMIISITAVA
MARERAWHLLVVPVYRIIYEPLRAYLLYASAYRAIKGTIVAWDKLERRNTVSAFVERHRPMPPILGAQQ
>Mature_789_residues
MFSPFRRRPDAIRGTDPLTAAPGPVFFDRTGKRLYYFAAGVIVLAVIVAVLPVRVTPMAFDPLWTVPTNSDSGFPRRVLS
TADDRRFPVLGSEDDEVLSRVVRVDRRGDQWNLVDPFTNQLYRVADDDDKDRIRDSAYAIDHYGRPPDRHLMLTFDDGPD
VEFTTQVLDILSREKIPATFFVLGSQVVLHPDVFRRMIREGHMAGNHTMFHVDFDEHTDFRNRQEIIATDRVMRATAAYA
SRLFRIPRGDPDNNTLALLQSQQLGYLQVDQDIDTLDWKVSPENEVAVPQLDGRGHVVLLHDGGGDRSATVRMLEQLIAE
AKSKGYTFSTLAPLLSEQELPVSNAEQYPADAATYHTLRLMEKAPGAVLGFLFWLGMGSLTVMSLLYLILALVCQYRQNR
LRWNDIGDDQLPMVSVVLAAFNEEKVIARTIAELRRSDYPRSRFEVVAVNDGSTDGTLRILTELARDWPKLRVVDQANSG
KSSAINNGINHASAVSTVMVTMDADTLFRPDTIRNLARHFARHTHGRQVGAVAGHIKVGNRRNLLTAWQSLEYISGICVT
RMAERLLNAISIVPGACSAWSRTALEEIGGFCDDTMAEDCDATLALQRRGYRILQENNAIADTEAPETIRALAKQRKRWT
YGNIQALWKHRAMLFRPRYGALGLVALPYAALSLIVPLLFMPLTIVAAGMSLAAGNWQSIALFAGFVAALHMIISITAVA
MARERAWHLLVVPVYRIIYEPLRAYLLYASAYRAIKGTIVAWDKLERRNTVSAFVERHRPMPPILGAQQ

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=411, Percent_Identity=27.4939172749392, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1786560, Length=254, Percent_Identity=30.3149606299213, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323339, Length=175, Percent_Identity=34.8571428571429, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323338, Length=167, Percent_Identity=32.9341317365269, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 88432; Mature: 88432

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSPFRRRPDAIRGTDPLTAAPGPVFFDRTGKRLYYFAAGVIVLAVIVAVLPVRVTPMAF
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHCCEECCEEC
DPLWTVPTNSDSGFPRRVLSTADDRRFPVLGSEDDEVLSRVVRVDRRGDQWNLVDPFTNQ
CCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
LYRVADDDDKDRIRDSAYAIDHYGRPPDRHLMLTFDDGPDVEFTTQVLDILSREKIPATF
HEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
FVLGSQVVLHPDVFRRMIREGHMAGNHTMFHVDFDEHTDFRNRQEIIATDRVMRATAAYA
EEECCCEEECHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
SRLFRIPRGDPDNNTLALLQSQQLGYLQVDQDIDTLDWKVSPENEVAVPQLDGRGHVVLL
HHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEECCCCCEECCEECCCCCEEEE
HDGGGDRSATVRMLEQLIAEAKSKGYTFSTLAPLLSEQELPVSNAEQYPADAATYHTLRL
ECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
MEKAPGAVLGFLFWLGMGSLTVMSLLYLILALVCQYRQNRLRWNDIGDDQLPMVSVVLAA
HHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
FNEEKVIARTIAELRRSDYPRSRFEVVAVNDGSTDGTLRILTELARDWPKLRVVDQANSG
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
KSSAINNGINHASAVSTVMVTMDADTLFRPDTIRNLARHFARHTHGRQVGAVAGHIKVGN
CCHHHHCCCCHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEECCEEEEEEECC
RRNLLTAWQSLEYISGICVTRMAERLLNAISIVPGACSAWSRTALEEIGGFCDDTMAEDC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHC
DATLALQRRGYRILQENNAIADTEAPETIRALAKQRKRWTYGNIQALWKHRAMLFRPRYG
CHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCC
ALGLVALPYAALSLIVPLLFMPLTIVAAGMSLAAGNWQSIALFAGFVAALHMIISITAVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MARERAWHLLVVPVYRIIYEPLRAYLLYASAYRAIKGTIVAWDKLERRNTVSAFVERHRP
HHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCC
MPPILGAQQ
CCCCCCCCC
>Mature Secondary Structure
MFSPFRRRPDAIRGTDPLTAAPGPVFFDRTGKRLYYFAAGVIVLAVIVAVLPVRVTPMAF
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHCCEECCEEC
DPLWTVPTNSDSGFPRRVLSTADDRRFPVLGSEDDEVLSRVVRVDRRGDQWNLVDPFTNQ
CCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
LYRVADDDDKDRIRDSAYAIDHYGRPPDRHLMLTFDDGPDVEFTTQVLDILSREKIPATF
HEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
FVLGSQVVLHPDVFRRMIREGHMAGNHTMFHVDFDEHTDFRNRQEIIATDRVMRATAAYA
EEECCCEEECHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
SRLFRIPRGDPDNNTLALLQSQQLGYLQVDQDIDTLDWKVSPENEVAVPQLDGRGHVVLL
HHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEECCCCCEECCEECCCCCEEEE
HDGGGDRSATVRMLEQLIAEAKSKGYTFSTLAPLLSEQELPVSNAEQYPADAATYHTLRL
ECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
MEKAPGAVLGFLFWLGMGSLTVMSLLYLILALVCQYRQNRLRWNDIGDDQLPMVSVVLAA
HHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
FNEEKVIARTIAELRRSDYPRSRFEVVAVNDGSTDGTLRILTELARDWPKLRVVDQANSG
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
KSSAINNGINHASAVSTVMVTMDADTLFRPDTIRNLARHFARHTHGRQVGAVAGHIKVGN
CCHHHHCCCCHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEECCEEEEEEECC
RRNLLTAWQSLEYISGICVTRMAERLLNAISIVPGACSAWSRTALEEIGGFCDDTMAEDC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHC
DATLALQRRGYRILQENNAIADTEAPETIRALAKQRKRWTYGNIQALWKHRAMLFRPRYG
CHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCC
ALGLVALPYAALSLIVPLLFMPLTIVAAGMSLAAGNWQSIALFAGFVAALHMIISITAVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MARERAWHLLVVPVYRIIYEPLRAYLLYASAYRAIKGTIVAWDKLERRNTVSAFVERHRP
HHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCC
MPPILGAQQ
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]