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The map label for this gene is asnB [H]

Identifier: 119869393

GI number: 119869393

Start: 3524601

End: 3526568

Strand: Direct

Name: asnB [H]

Synonym: Mkms_3361

Alternate gene names: 119869393

Gene position: 3524601-3526568 (Clockwise)

Preceding gene: 119869388

Following gene: 119869395

Centisome position: 61.43

GC content: 69.46

Gene sequence:

>1968_bases
GTGTGCGGACTGCTGGCGTTCCTGAAAGACCCGTCCGCGCAGTCCTCCCCCGCGCTGGTCGGCGCGGTCTCGGGGGCTGC
TGAACTGATGCGGCACCGGGGGCCCGACGAACCGGGCACCTGGGCGGACGACACCGATGACCCGGGCATCGTGCTGGGCT
TCAATCGGCTGTCGATCATCGACATCGCGCACAGTCACCAACCGCTGCGCTGGGGTCCGCCCGAGGCGCCGCTGCGCTAC
GTCCTGGTGTTCAACGGGGAGATCTACAACTACCTGGAACTCCGTGAGGCGTTGCGCACCGAACACGGCGCCGAGTTCGC
CACCGACGGGGACGGCGAGGCGATCGTCGCGGCCTACCACCACTGGGGCCCTGCGGCGCTGTCGCGGCTGCGCGGCATGT
TCGCGTTCGCGCTGTGGGACACCGTCACCGGTGAGCTGTTCTGCGCACGGGATCCGTTCGGCATCAAACCGCTCTACATC
GCCACCGGCCCCGGTGGCACCGCGGTCGGCAGCGAGAAGAAGTGCCTGATGGCCGTCGCCGACCAGCTCGGCATCGACAC
CGGCCTCGACGAGCGTGCGGTGCAGCACTACACCGTGCTGCAGTACGTGCCCGAGCCCGAGACGCTGCACCGCGGCGTGC
GCCGGCTCGAGTCGGGCAGCTACCTGCGGATGCGCCCGGGGCAGGCTCCGGTGGTCACCCGCTACTTCACCCCCCGCTTC
GACGCGGTTCCTTTTCGCGCCGCTTCCGCGCCTGGTTCGGCGGGCGGCGTCGATCAGGCCCGCTACGACGAGATCACCGC
GGTACTCGAGGACTCGGTGGCCAAACACATGCGCGCCGACGTCACCGTCGGCGCCTTCCTGTCCGGCGGCATCGACTCCA
CCGCCATCGCCGCGTTGGCGATGCGCCACAACCCGCGCCTGATCACATTCACCACCGGATTCGAGCGCGAGGGGTTCTCG
GAGGTCGACGTCGCGGTGGCGTCGGCGGAGGCGATCGGCGCCCGGCACGTCACCAAGGTGGTCAGCCAGGCGGAGTTCGT
CGAGGCGCTGCCCGCGATCGTCTGGTATCTCGACGAGCCGGTGGCCGATCCGGCGCTGGTACCGCTGTACTTCATCGCCC
GCGAGGCGCGTAAGCACGTCAAGGTGGTGCTCTCCGGGGAGGGCGCCGACGAGTTGTTCGGCGGCTACACCATCTACCGC
GAACCATTGTCGCTGCGGGCGTTCGACTACCTGCCGCGGCCGGTCCGCCGCTCGTTGCGCCGGGCGTCGGCCCCGCTCCC
CGAGGGGATGCGCGGCAAGAGCCTGCTGCACCGCGGTTCGCAGACCCTCGAGGAGCGGTACTACGGCAACGCCCGCAGTT
TCTCCGACGAGCACCTGCGCGCGGTGCTGCGCCGCTTCTCGCCGGACTGGACGCACACCGACGTCACCGCCGCGCTCTAC
CGGGAGTCCGACGGCTGGGATCCGGTCGCGCGCATGCAGCACATCGACCTGTTCACCTGGCTGCGCGGCGACATCCTGGT
CAAGGCCGACAAGATGACGATGGCCAACTCGCTGGAGCTGCGGGTGCCGTTCCTCGATCCCGAGGTGTTCGCGGTCGCGT
CGCGGCTGCCGCTGGAGCAGAAGATCACCCGGGCCACCACGAAGTTCGCCCTGCGGCGCGCGCTGGAACCGGTCGTGCCC
GCACACGTGCTCAACCGCCCGAAACTCGGCTTCCCGGTCCCGATCCGGCACTGGTTGCGTGCCGGGGAACTGCTGGAATG
GGCCTACGAGACGGTCGCCGCGTCACAGACGGGCGATCTCATCGACGCCGAGGCGGTGCGCACGATGCTCGACGAGCACC
GGACCGGGGTCAGCGATCACAGCCGCCGGTTGTGGACCGTGCTGATCTTCATGCTCTGGCATGCGATCTTCGTCGAGGGC
AGTGTGACTCCCCGGATCGAGGAGCCGCACTACCCCGTCCAGCTTTAG

Upstream 100 bases:

>100_bases
TACTACGCAGCGTAGACCACGCCGGGGTGGCGGTCTCGAGACACCCGACCTTTACGTGCCGTCGTGCCCCACCACCGCCG
CATTGGGCATACTTGGCGCC

Downstream 100 bases:

>100_bases
GCCAGAGCCGAGGCGATCTCCTTGGCGGCGTCTTCGCCGTAGGCGTCGGCCAGGCGGGTGATACCGGCGTCTTTGTCCCA
GCGCCACTCCTGCGGGCCGG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MCGLLAFLKDPSAQSSPALVGAVSGAAELMRHRGPDEPGTWADDTDDPGIVLGFNRLSIIDIAHSHQPLRWGPPEAPLRY
VLVFNGEIYNYLELREALRTEHGAEFATDGDGEAIVAAYHHWGPAALSRLRGMFAFALWDTVTGELFCARDPFGIKPLYI
ATGPGGTAVGSEKKCLMAVADQLGIDTGLDERAVQHYTVLQYVPEPETLHRGVRRLESGSYLRMRPGQAPVVTRYFTPRF
DAVPFRAASAPGSAGGVDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAMRHNPRLITFTTGFEREGFS
EVDVAVASAEAIGARHVTKVVSQAEFVEALPAIVWYLDEPVADPALVPLYFIAREARKHVKVVLSGEGADELFGGYTIYR
EPLSLRAFDYLPRPVRRSLRRASAPLPEGMRGKSLLHRGSQTLEERYYGNARSFSDEHLRAVLRRFSPDWTHTDVTAALY
RESDGWDPVARMQHIDLFTWLRGDILVKADKMTMANSLELRVPFLDPEVFAVASRLPLEQKITRATTKFALRRALEPVVP
AHVLNRPKLGFPVPIRHWLRAGELLEWAYETVAASQTGDLIDAEAVRTMLDEHRTGVSDHSRRLWTVLIFMLWHAIFVEG
SVTPRIEEPHYPVQL

Sequences:

>Translated_655_residues
MCGLLAFLKDPSAQSSPALVGAVSGAAELMRHRGPDEPGTWADDTDDPGIVLGFNRLSIIDIAHSHQPLRWGPPEAPLRY
VLVFNGEIYNYLELREALRTEHGAEFATDGDGEAIVAAYHHWGPAALSRLRGMFAFALWDTVTGELFCARDPFGIKPLYI
ATGPGGTAVGSEKKCLMAVADQLGIDTGLDERAVQHYTVLQYVPEPETLHRGVRRLESGSYLRMRPGQAPVVTRYFTPRF
DAVPFRAASAPGSAGGVDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAMRHNPRLITFTTGFEREGFS
EVDVAVASAEAIGARHVTKVVSQAEFVEALPAIVWYLDEPVADPALVPLYFIAREARKHVKVVLSGEGADELFGGYTIYR
EPLSLRAFDYLPRPVRRSLRRASAPLPEGMRGKSLLHRGSQTLEERYYGNARSFSDEHLRAVLRRFSPDWTHTDVTAALY
RESDGWDPVARMQHIDLFTWLRGDILVKADKMTMANSLELRVPFLDPEVFAVASRLPLEQKITRATTKFALRRALEPVVP
AHVLNRPKLGFPVPIRHWLRAGELLEWAYETVAASQTGDLIDAEAVRTMLDEHRTGVSDHSRRLWTVLIFMLWHAIFVEG
SVTPRIEEPHYPVQL
>Mature_655_residues
MCGLLAFLKDPSAQSSPALVGAVSGAAELMRHRGPDEPGTWADDTDDPGIVLGFNRLSIIDIAHSHQPLRWGPPEAPLRY
VLVFNGEIYNYLELREALRTEHGAEFATDGDGEAIVAAYHHWGPAALSRLRGMFAFALWDTVTGELFCARDPFGIKPLYI
ATGPGGTAVGSEKKCLMAVADQLGIDTGLDERAVQHYTVLQYVPEPETLHRGVRRLESGSYLRMRPGQAPVVTRYFTPRF
DAVPFRAASAPGSAGGVDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAMRHNPRLITFTTGFEREGFS
EVDVAVASAEAIGARHVTKVVSQAEFVEALPAIVWYLDEPVADPALVPLYFIAREARKHVKVVLSGEGADELFGGYTIYR
EPLSLRAFDYLPRPVRRSLRRASAPLPEGMRGKSLLHRGSQTLEERYYGNARSFSDEHLRAVLRRFSPDWTHTDVTAALY
RESDGWDPVARMQHIDLFTWLRGDILVKADKMTMANSLELRVPFLDPEVFAVASRLPLEQKITRATTKFALRRALEPVVP
AHVLNRPKLGFPVPIRHWLRAGELLEWAYETVAASQTGDLIDAEAVRTMLDEHRTGVSDHSRRLWTVLIFMLWHAIFVEG
SVTPRIEEPHYPVQL

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=413, Percent_Identity=28.0871670702179, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI168229252, Length=413, Percent_Identity=28.0871670702179, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI168229250, Length=413, Percent_Identity=28.0871670702179, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI296010848, Length=382, Percent_Identity=28.5340314136126, Blast_Score=120, Evalue=6e-27,
Organism=Escherichia coli, GI1786889, Length=395, Percent_Identity=30.8860759493671, Blast_Score=156, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI25147557, Length=418, Percent_Identity=29.9043062200957, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71993933, Length=418, Percent_Identity=30.1435406698565, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560178, Length=422, Percent_Identity=26.7772511848341, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25147560, Length=344, Percent_Identity=28.1976744186047, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321563, Length=446, Percent_Identity=27.8026905829596, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325403, Length=445, Percent_Identity=27.1910112359551, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45553209, Length=426, Percent_Identity=29.5774647887324, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72654; Mature: 72654

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLLAFLKDPSAQSSPALVGAVSGAAELMRHRGPDEPGTWADDTDDPGIVLGFNRLSII
CCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEE
DIAHSHQPLRWGPPEAPLRYVLVFNGEIYNYLELREALRTEHGAEFATDGDGEAIVAAYH
EECCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
HWGPAALSRLRGMFAFALWDTVTGELFCARDPFGIKPLYIATGPGGTAVGSEKKCLMAVA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHH
DQLGIDTGLDERAVQHYTVLQYVPEPETLHRGVRRLESGSYLRMRPGQAPVVTRYFTPRF
HHHCCCCCCCHHHHHHHHHEEECCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCC
DAVPFRAASAPGSAGGVDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCHHHHHHHH
MRHNPRLITFTTGFEREGFSEVDVAVASAEAIGARHVTKVVSQAEFVEALPAIVWYLDEP
HCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VADPALVPLYFIAREARKHVKVVLSGEGADELFGGYTIYREPLSLRAFDYLPRPVRRSLR
CCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEEEECCCCHHHHHCCCHHHHHHHH
RASAPLPEGMRGKSLLHRGSQTLEERYYGNARSFSDEHLRAVLRRFSPDWTHTDVTAALY
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHEE
RESDGWDPVARMQHIDLFTWLRGDILVKADKMTMANSLELRVPFLDPEVFAVASRLPLEQ
ECCCCCCHHHHHHHHHHHHHHCCCEEEEECCEEECCCCEEEEECCCHHHHHHHHCCCHHH
KITRATTKFALRRALEPVVPAHVLNRPKLGFPVPIRHWLRAGELLEWAYETVAASQTGDL
HHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
IDAEAVRTMLDEHRTGVSDHSRRLWTVLIFMLWHAIFVEGSVTPRIEEPHYPVQL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGLLAFLKDPSAQSSPALVGAVSGAAELMRHRGPDEPGTWADDTDDPGIVLGFNRLSII
CCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEE
DIAHSHQPLRWGPPEAPLRYVLVFNGEIYNYLELREALRTEHGAEFATDGDGEAIVAAYH
EECCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEC
HWGPAALSRLRGMFAFALWDTVTGELFCARDPFGIKPLYIATGPGGTAVGSEKKCLMAVA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHH
DQLGIDTGLDERAVQHYTVLQYVPEPETLHRGVRRLESGSYLRMRPGQAPVVTRYFTPRF
HHHCCCCCCCHHHHHHHHHEEECCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCC
DAVPFRAASAPGSAGGVDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCHHHHHHHH
MRHNPRLITFTTGFEREGFSEVDVAVASAEAIGARHVTKVVSQAEFVEALPAIVWYLDEP
HCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VADPALVPLYFIAREARKHVKVVLSGEGADELFGGYTIYREPLSLRAFDYLPRPVRRSLR
CCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEEEECCCCHHHHHCCCHHHHHHHH
RASAPLPEGMRGKSLLHRGSQTLEERYYGNARSFSDEHLRAVLRRFSPDWTHTDVTAALY
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHEE
RESDGWDPVARMQHIDLFTWLRGDILVKADKMTMANSLELRVPFLDPEVFAVASRLPLEQ
ECCCCCCHHHHHHHHHHHHHHCCCEEEEECCEEECCCCEEEEECCCHHHHHHHHCCCHHH
KITRATTKFALRRALEPVVPAHVLNRPKLGFPVPIRHWLRAGELLEWAYETVAASQTGDL
HHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
IDAEAVRTMLDEHRTGVSDHSRRLWTVLIFMLWHAIFVEGSVTPRIEEPHYPVQL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]