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The map label for this gene is ansA [H]

Identifier: 119869186

GI number: 119869186

Start: 3301019

End: 3301948

Strand: Direct

Name: ansA [H]

Synonym: Mkms_3154

Alternate gene names: 119869186

Gene position: 3301019-3301948 (Clockwise)

Preceding gene: 119869177

Following gene: 119869188

Centisome position: 57.54

GC content: 75.81

Gene sequence:

>930_bases
GTGAGCCTTGTCGTGATCACCACCGGCGGCACGATCGCCACCAGCACCGACGCCGACGGCGTACGCCGGCCCACCCGCCG
CGGCGCCGACCTGACCGCCGGTCTGGACGTGACGGTGGTCGACGTGCTCAACAAGGACAGCTCGCAGCTCACCCCCGCCG
ACTGGGACCGCATCCGCGACGCGGTGCTGGCCGGCACGGCGGGCGGCGCCGCCGACGGCGTCGTCGTCACCCACGGCACC
GACACCATGGAGGAGACGGCGCTGTGGCTCGAACTCACCTACGACGGCACCACCCCGGTGGTGCTGACCGGTGCGCAGCG
CGGGGCCGACGCACCCGATGCCGACGGCCCGGCCAACCTGCGGGACGCGGTGACGGTCGCCGGCGATCCCGAGGCCCGCG
GACTCGGGGTGCTCGTCAGCTTCGCCGGTACGGTCCGCCGGCCGCTGGGCCTGCGCAAGGTGGCGACCGAGGACCTCGAC
GGTTTCGACGGACCGGTGGTGGGTCGGGTCGCCGACGGCCGGTTCACCGTGACCGGGCCCAAGGTGCGTCCGTACCTGGG
CGACGTGTCTGCGGAACGCGCACCGCGCGTCGACATCGTCACCACCTATCCGGGCGGCGACGCCGCCGCCCTGTCCGCTT
GCGCGGCCGCCGGGGCGCGGGCGGTCGTCCTCGAGGCGCTGGGTGCCGGCAACGCCGCGCAACCGGTGATCGACGGGGTA
CGCCGGCTGTGCGCCGACGGGGTGCTCGTGGCGGTGTCGACGCGGGTGCCGGGTGGGCGGGTCCGTCCCGCCTACGGTCC
CGGGCGCGACCTCGCCGACGCGGGTGCGCTCATGGTGCCGACCCTGCGGCCCGCGCAGGCGCGGGTGCTGCTGATGGCCG
CACTCGCCGCGGACGTCCCGGCCGCCGAGGTGCTCGCCGAATGGGGCTGA

Upstream 100 bases:

>100_bases
CGGCGCCGGTCGTGGGCTCCACCTTGCCCGATGCCTTGCCCGGTGTTTCGTCAGTCACCACACCATCATCCCAAACGGTC
GATACGGGACAATTCCGCAC

Downstream 100 bases:

>100_bases
CGTGTCAGGGCAGGTCGGCCTGCGGGAGCGCGTCCACGTAGTCCGCCCAGTCGAGGCTGTCGACCGGATTGGCCCGGACG
ACGTCGGGCACCGTGTCGGG

Product: asparaginase/glutaminase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSLVVITTGGTIATSTDADGVRRPTRRGADLTAGLDVTVVDVLNKDSSQLTPADWDRIRDAVLAGTAGGAADGVVVTHGT
DTMEETALWLELTYDGTTPVVLTGAQRGADAPDADGPANLRDAVTVAGDPEARGLGVLVSFAGTVRRPLGLRKVATEDLD
GFDGPVVGRVADGRFTVTGPKVRPYLGDVSAERAPRVDIVTTYPGGDAAALSACAAAGARAVVLEALGAGNAAQPVIDGV
RRLCADGVLVAVSTRVPGGRVRPAYGPGRDLADAGALMVPTLRPAQARVLLMAALAADVPAAEVLAEWG

Sequences:

>Translated_309_residues
MSLVVITTGGTIATSTDADGVRRPTRRGADLTAGLDVTVVDVLNKDSSQLTPADWDRIRDAVLAGTAGGAADGVVVTHGT
DTMEETALWLELTYDGTTPVVLTGAQRGADAPDADGPANLRDAVTVAGDPEARGLGVLVSFAGTVRRPLGLRKVATEDLD
GFDGPVVGRVADGRFTVTGPKVRPYLGDVSAERAPRVDIVTTYPGGDAAALSACAAAGARAVVLEALGAGNAAQPVIDGV
RRLCADGVLVAVSTRVPGGRVRPAYGPGRDLADAGALMVPTLRPAQARVLLMAALAADVPAAEVLAEWG
>Mature_308_residues
SLVVITTGGTIATSTDADGVRRPTRRGADLTAGLDVTVVDVLNKDSSQLTPADWDRIRDAVLAGTAGGAADGVVVTHGTD
TMEETALWLELTYDGTTPVVLTGAQRGADAPDADGPANLRDAVTVAGDPEARGLGVLVSFAGTVRRPLGLRKVATEDLDG
FDGPVVGRVADGRFTVTGPKVRPYLGDVSAERAPRVDIVTTYPGGDAAALSACAAAGARAVVLEALGAGNAAQPVIDGVR
RLCADGVLVAVSTRVPGGRVRPAYGPGRDLADAGALMVPTLRPAQARVLLMAALAADVPAAEVLAEWG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=233, Percent_Identity=35.6223175965665, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788065, Length=295, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193207372, Length=245, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=245, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558280, Length=245, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558278, Length=245, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320527, Length=328, Percent_Identity=27.7439024390244, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323189, Length=237, Percent_Identity=31.6455696202532, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323187, Length=237, Percent_Identity=31.6455696202532, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323186, Length=237, Percent_Identity=31.6455696202532, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323184, Length=237, Percent_Identity=31.6455696202532, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24645530, Length=247, Percent_Identity=28.3400809716599, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639609, Length=247, Percent_Identity=28.7449392712551, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 31288; Mature: 31156

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVVITTGGTIATSTDADGVRRPTRRGADLTAGLDVTVVDVLNKDSSQLTPADWDRIRD
CEEEEEECCCEEEECCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCCHHHHHHH
AVLAGTAGGAADGVVVTHGTDTMEETALWLELTYDGTTPVVLTGAQRGADAPDADGPANL
HHHCCCCCCCCCCEEEECCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCC
RDAVTVAGDPEARGLGVLVSFAGTVRRPLGLRKVATEDLDGFDGPVVGRVADGRFTVTGP
CCEEEECCCCCCCCCEEEEEHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEECCEEEEECC
KVRPYLGDVSAERAPRVDIVTTYPGGDAAALSACAAAGARAVVLEALGAGNAAQPVIDGV
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHH
RRLCADGVLVAVSTRVPGGRVRPAYGPGRDLADAGALMVPTLRPAQARVLLMAALAADVP
HHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCC
AAEVLAEWG
HHHHHHCCC
>Mature Secondary Structure 
SLVVITTGGTIATSTDADGVRRPTRRGADLTAGLDVTVVDVLNKDSSQLTPADWDRIRD
EEEEEECCCEEEECCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCCHHHHHHH
AVLAGTAGGAADGVVVTHGTDTMEETALWLELTYDGTTPVVLTGAQRGADAPDADGPANL
HHHCCCCCCCCCCEEEECCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCC
RDAVTVAGDPEARGLGVLVSFAGTVRRPLGLRKVATEDLDGFDGPVVGRVADGRFTVTGP
CCEEEECCCCCCCCCEEEEEHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEECCEEEEECC
KVRPYLGDVSAERAPRVDIVTTYPGGDAAALSACAAAGARAVVLEALGAGNAAQPVIDGV
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHH
RRLCADGVLVAVSTRVPGGRVRPAYGPGRDLADAGALMVPTLRPAQARVLLMAALAADVP
HHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCC
AAEVLAEWG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]