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The map label for this gene is nplT [H]

Identifier: 119868847

GI number: 119868847

Start: 2935043

End: 2936344

Strand: Direct

Name: nplT [H]

Synonym: Mkms_2815

Alternate gene names: 119868847

Gene position: 2935043-2936344 (Clockwise)

Preceding gene: 119868846

Following gene: 119868849

Centisome position: 51.16

GC content: 69.12

Gene sequence:

>1302_bases
ATGACAGAACCCGCGTGGGTGGAACACGTCATCTGGTGGCAGATCTACCCGCTGGGGTTCGTCGGCGCCTTCCCCGCCGA
TCCCCCACCCACACCCGACGAGCACCGGTTGCGGCGCATCGTGGACTGGCTCGACCACGCCATCGCGCTCGGCGCGTCCG
GTCTGGCGCTGGGGCCCGTATTCGCCTCGCGCACACACGGATACGACACCACCGACCACTATTCGATAGATCCGCGACTC
GGTGACGACGGCGACTTCGACGAACTGGTCGACCAGGCGCACCGACGCGGTCTTCGGGTTCTGCTCGACGGCGTGTTCAA
CCACGTCGGCACCGACTTCCCCCGCTACCGCGAAGCACTCGACGGCGGACCCGAGCATCCCTCGTCGGCCTGGTTTCGGC
GCCGGCGCAACGGCTTCGACACGTTCGAGGGCCACGGCGAGCTGATCGCGCTCAACCACCGCGAGCCCGCCGTCGTCGAC
TACACCGTCGAGGTGATGTCGCACTGGCTCGCCCGTGGGGCCGACGGCTGGCGGCTCGACGCCGCATACGCCGTCCCGGA
CGGGTTCTGGTCCCAGGTGCTGCCGCGGGTGCGCGAGGCGCATCCCGACGCGTGGTTCGTCGGCGAGGTGATCCACGGTG
ACTACGCGGGCCGGGTGGCGGCCGGTTTCGACTCCGTCACCCAGTACGAGCTGTGGAAGGCGGTGTGGAGCAGCCTCAAC
GACGGCAACTTCCACGAACTCGACCACGCCTTGGCGCGGCACGACGAGTTCCTCGCCACGTTCGTGCCGCTGACGTTCGT
CGGCAACCACGACGTCACCCGCATCGCGAGCCGGATGGACGATCCCCGCCACCTCGAACAGGCGCTGGTGCTGCTGTTCA
CGATCGGCGGGGTGCCAAGCGTCTACGCCGGCGACGAGCTCGGATACCGCGGCGTCAAGGAGGACCGGGCCGGCGGTGAC
GACGCGGTGCGACCCGAATTCGGATCGCCGCCAACCGAAGACAGCGATATCTTCCGGCTGCACAAGTACCTGATCGGACT
GCGCCGCCGTCATCCGTGGCTGCACTGGGCGACGACCTCGGCGGTGCAGCTGACGAACCGGCAGTACGTCTACGAGACCC
GCCACGGTGACGATGCGCTCGTGGTGGCACTCAACGTCGACGACGCACCGCTGACGATCTCGGTCGGCGACACCGCGCTG
CGCCGTGGCCGGGTGGTGGCCGGATCCGGGGCGCCACCCGCGGAGGAACTCGACCGGCTGACCGTGCCGCCGCACGGATG
GGTGGTCGTCGCACCCGCTTGA

Upstream 100 bases:

>100_bases
CCCTGCCCGTCAACACCGTCGCACCCGGCGTGCCGGTGGACGGACCGGCGCCGGAACTGGCCGACGCCATGCAAGCGCTC
TATCGGGAGAAGTTCGGCTC

Downstream 100 bases:

>100_bases
GCGGTCACAGCCGCGAGACGGTCGCGGGGTCCGGTTCCCCGCCGTAGAACTTGTCGAGGACCGCGAGGAAATCGGCGTTG
TCGTCGGCGCACCGGGCGAA

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTEPAWVEHVIWWQIYPLGFVGAFPADPPPTPDEHRLRRIVDWLDHAIALGASGLALGPVFASRTHGYDTTDHYSIDPRL
GDDGDFDELVDQAHRRGLRVLLDGVFNHVGTDFPRYREALDGGPEHPSSAWFRRRRNGFDTFEGHGELIALNHREPAVVD
YTVEVMSHWLARGADGWRLDAAYAVPDGFWSQVLPRVREAHPDAWFVGEVIHGDYAGRVAAGFDSVTQYELWKAVWSSLN
DGNFHELDHALARHDEFLATFVPLTFVGNHDVTRIASRMDDPRHLEQALVLLFTIGGVPSVYAGDELGYRGVKEDRAGGD
DAVRPEFGSPPTEDSDIFRLHKYLIGLRRRHPWLHWATTSAVQLTNRQYVYETRHGDDALVVALNVDDAPLTISVGDTAL
RRGRVVAGSGAPPAEELDRLTVPPHGWVVVAPA

Sequences:

>Translated_433_residues
MTEPAWVEHVIWWQIYPLGFVGAFPADPPPTPDEHRLRRIVDWLDHAIALGASGLALGPVFASRTHGYDTTDHYSIDPRL
GDDGDFDELVDQAHRRGLRVLLDGVFNHVGTDFPRYREALDGGPEHPSSAWFRRRRNGFDTFEGHGELIALNHREPAVVD
YTVEVMSHWLARGADGWRLDAAYAVPDGFWSQVLPRVREAHPDAWFVGEVIHGDYAGRVAAGFDSVTQYELWKAVWSSLN
DGNFHELDHALARHDEFLATFVPLTFVGNHDVTRIASRMDDPRHLEQALVLLFTIGGVPSVYAGDELGYRGVKEDRAGGD
DAVRPEFGSPPTEDSDIFRLHKYLIGLRRRHPWLHWATTSAVQLTNRQYVYETRHGDDALVVALNVDDAPLTISVGDTAL
RRGRVVAGSGAPPAEELDRLTVPPHGWVVVAPA
>Mature_432_residues
TEPAWVEHVIWWQIYPLGFVGAFPADPPPTPDEHRLRRIVDWLDHAIALGASGLALGPVFASRTHGYDTTDHYSIDPRLG
DDGDFDELVDQAHRRGLRVLLDGVFNHVGTDFPRYREALDGGPEHPSSAWFRRRRNGFDTFEGHGELIALNHREPAVVDY
TVEVMSHWLARGADGWRLDAAYAVPDGFWSQVLPRVREAHPDAWFVGEVIHGDYAGRVAAGFDSVTQYELWKAVWSSLND
GNFHELDHALARHDEFLATFVPLTFVGNHDVTRIASRMDDPRHLEQALVLLFTIGGVPSVYAGDELGYRGVKEDRAGGDD
AVRPEFGSPPTEDSDIFRLHKYLIGLRRRHPWLHWATTSAVQLTNRQYVYETRHGDDALVVALNVDDAPLTISVGDTALR
RGRVVAGSGAPPAEELDRLTVPPHGWVVVAPA

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=392, Percent_Identity=26.2755102040816, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790687, Length=212, Percent_Identity=34.4339622641509, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221330053, Length=229, Percent_Identity=30.1310043668122, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583749, Length=397, Percent_Identity=24.4332493702771, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24583747, Length=394, Percent_Identity=24.3654822335025, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549022, Length=208, Percent_Identity=28.3653846153846, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586587, Length=208, Percent_Identity=27.8846153846154, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24586597, Length=230, Percent_Identity=27.3913043478261, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24586591, Length=385, Percent_Identity=22.5974025974026, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586593, Length=209, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 48346; Mature: 48215

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPAWVEHVIWWQIYPLGFVGAFPADPPPTPDEHRLRRIVDWLDHAIALGASGLALGPV
CCCCHHHHHEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
FASRTHGYDTTDHYSIDPRLGDDGDFDELVDQAHRRGLRVLLDGVFNHVGTDFPRYREAL
HHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
DGGPEHPSSAWFRRRRNGFDTFEGHGELIALNHREPAVVDYTVEVMSHWLARGADGWRLD
CCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHCCCCCCEEE
AAYAVPDGFWSQVLPRVREAHPDAWFVGEVIHGDYAGRVAAGFDSVTQYELWKAVWSSLN
EEEECCCHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCC
DGNFHELDHALARHDEFLATFVPLTFVGNHDVTRIASRMDDPRHLEQALVLLFTIGGVPS
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
VYAGDELGYRGVKEDRAGGDDAVRPEFGSPPTEDSDIFRLHKYLIGLRRRHPWLHWATTS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCC
AVQLTNRQYVYETRHGDDALVVALNVDDAPLTISVGDTALRRGRVVAGSGAPPAEELDRL
EEEECCCEEEEEECCCCCEEEEEEECCCCCEEEEECHHHHHCCEEEECCCCCCHHHHCCC
TVPPHGWVVVAPA
CCCCCCEEEEECC
>Mature Secondary Structure 
TEPAWVEHVIWWQIYPLGFVGAFPADPPPTPDEHRLRRIVDWLDHAIALGASGLALGPV
CCCHHHHHEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
FASRTHGYDTTDHYSIDPRLGDDGDFDELVDQAHRRGLRVLLDGVFNHVGTDFPRYREAL
HHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
DGGPEHPSSAWFRRRRNGFDTFEGHGELIALNHREPAVVDYTVEVMSHWLARGADGWRLD
CCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHCCCCCCEEE
AAYAVPDGFWSQVLPRVREAHPDAWFVGEVIHGDYAGRVAAGFDSVTQYELWKAVWSSLN
EEEECCCHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCC
DGNFHELDHALARHDEFLATFVPLTFVGNHDVTRIASRMDDPRHLEQALVLLFTIGGVPS
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
VYAGDELGYRGVKEDRAGGDDAVRPEFGSPPTEDSDIFRLHKYLIGLRRRHPWLHWATTS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCC
AVQLTNRQYVYETRHGDDALVVALNVDDAPLTISVGDTALRRGRVVAGSGAPPAEELDRL
EEEECCCEEEEEECCCCCEEEEEEECCCCCEEEEECHHHHHCCEEEECCCCCCHHHHCCC
TVPPHGWVVVAPA
CCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]