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The map label for this gene is lcfB [H]

Identifier: 119868744

GI number: 119868744

Start: 2833969

End: 2835588

Strand: Direct

Name: lcfB [H]

Synonym: Mkms_2711

Alternate gene names: 119868744

Gene position: 2833969-2835588 (Clockwise)

Preceding gene: 119868742

Following gene: 119868749

Centisome position: 49.4

GC content: 71.23

Gene sequence:

>1620_bases
GTGGATCAACCTCCGGCGCGACCCCGCGGTGGCCGGTGTGATCCCGTCGGAGAAGATCGGCAGGTGATCCCGCCGCGCCC
CACCATCCCGGCTCTCCTGGCGCGCAGCGCCCGCGAGTTCGGAGACCAGGCCTACGTGATCTCCCCGACCGAGCGGCTCA
CCTACGGCGATGCCGAACGGCGCTCGGCGGAGGTCGCCCGCTGGTTGCTCGGCCGGGGCGTCGGCAAGGGTGTGCGCGTC
GGTCTGTTCTTCCCCAACGGGGTGGACTGGATCGTCTGGTGGCTGGCGGTCTCACGCATCGGAGCGCTGGCCGTACCGCT
GAGCACCATGTACACACCAGCTGAGCTGGCCAAGGTGGTGCGGCTGGCCGACGTCGCGCTGGTGGTCGGCCCGTCGGCGG
TGCTCGGGATCGACGTCGCGCAGCGTTTCGAGGAGGCGTTCCCGGGGCTGGCCGATCAGCCCGCCGGCCGCCTTGCGCTG
ACGCAGGCGCCCTATCTGCGCAGTGTCGTGCTGACCGGTGGGGCCGACCGCCCCTGGGTGACACCTCACGATTCGTCGGA
GGCGACGGTGTCCCGCGAGGTGCTGGCGGCGGTCGAAGACGAGGTGTCCCCGGCGGACCTGGCGGTGATGGTGCACACCT
CCGGGTCGACGGCCGATCCCAAAGGCGTGCTGCACACCCACGGCACCGTGGTCCGGCAGACGTCGGGGTGGCCGGCCGCG
GTGCGCGCGGTCACCGGATCCGCGGGGCCGGTGCGGATCCTGTGTGCGATGCCGTTCTTCTGGATCGGCGGCCTGCTGGC
GGCGACGGGTGCGCTGCACGAACCCGTCACCGTGCTGGTGCTGCCGCGACTCGACGCGCAGACCGCACTCGACCTGATCG
AGCGGGAACGGGCCACCGGGCTGGTGGGGTGGCCGGCCTTCACCCAGCGGCTGCGCGACCACCCCTCGTTCGCCGCCCGC
GACCTGAGCAGCGCGCCGATGCTGCGGGACGGCCCGCTCGACATCGCGATGACCGACGTGCCGGACGGCTATCCCGTACA
TCGGACCATGTCGGAGACCGCGGGCGGGTTCGCCTTCACGGAGATGGCGATCGTCGACGACGAGGGCCAGGCGGTTCCGG
AGGGCACCGTCGGCGAACTCCTCGTCCGCGGTATCGGCGTGATGGCCGGCTACAACAAGCGCGAACGCGCGGAGACCTTC
GACGCCGACGGCTGGTACCACACCGGCGACCGCGTCTACCGCAGAGAAGGAGACCCGCGGCTGTTCTACGTCGGACGCAC
CAGCGAACTGATCAAGGCCGCGGGCGCGAACGTCTCGCCGCTGGAGGTGGAGGCGGTCGTCGAGCAGTTCCCCGATGTGG
TGCAGTGCGTGGTGGTCGGCGTCGAAGACCCCGAGCGGGGTGAACAGGTGTGCGCCGCCGTCGTACCCGCGCGCGGCGAA
ATCGACGTCACGGATCTGTCCGCCCGGGCGCGCACCCAACTCTCGGCCTACAAGGTGCCCACCCGATGGGCCGTGGTCGG
GGCGGATCAGCTGCCCGTGCTGGCGAGCGGCAAACTCGATCGCAAGGCGGTCAAGAAGATGATCGCCGACGGTGTGCTGC
CGTCGGTGGCGCCGACCTAG

Upstream 100 bases:

>100_bases
TCGACCGGAGCCCGGTGCACAGAGTCGCCGCTGCCCGGATCGAACGTCATGGCCGTATCAACGTGTGTGTGACAGCCGCT
GTTCCGGTACCGGGAGTGAG

Downstream 100 bases:

>100_bases
CGCTCCCGCGGTGAGGGAGGGGCCGCTCCCCCGTCCTCGGGCTGGAACTCCGCGGACCCGTCGTCCTCCGTGGCGTCGAC
GTGGTCTCCGGCCTCAGGCA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MDQPPARPRGGRCDPVGEDRQVIPPRPTIPALLARSAREFGDQAYVISPTERLTYGDAERRSAEVARWLLGRGVGKGVRV
GLFFPNGVDWIVWWLAVSRIGALAVPLSTMYTPAELAKVVRLADVALVVGPSAVLGIDVAQRFEEAFPGLADQPAGRLAL
TQAPYLRSVVLTGGADRPWVTPHDSSEATVSREVLAAVEDEVSPADLAVMVHTSGSTADPKGVLHTHGTVVRQTSGWPAA
VRAVTGSAGPVRILCAMPFFWIGGLLAATGALHEPVTVLVLPRLDAQTALDLIERERATGLVGWPAFTQRLRDHPSFAAR
DLSSAPMLRDGPLDIAMTDVPDGYPVHRTMSETAGGFAFTEMAIVDDEGQAVPEGTVGELLVRGIGVMAGYNKRERAETF
DADGWYHTGDRVYRREGDPRLFYVGRTSELIKAAGANVSPLEVEAVVEQFPDVVQCVVVGVEDPERGEQVCAAVVPARGE
IDVTDLSARARTQLSAYKVPTRWAVVGADQLPVLASGKLDRKAVKKMIADGVLPSVAPT

Sequences:

>Translated_539_residues
MDQPPARPRGGRCDPVGEDRQVIPPRPTIPALLARSAREFGDQAYVISPTERLTYGDAERRSAEVARWLLGRGVGKGVRV
GLFFPNGVDWIVWWLAVSRIGALAVPLSTMYTPAELAKVVRLADVALVVGPSAVLGIDVAQRFEEAFPGLADQPAGRLAL
TQAPYLRSVVLTGGADRPWVTPHDSSEATVSREVLAAVEDEVSPADLAVMVHTSGSTADPKGVLHTHGTVVRQTSGWPAA
VRAVTGSAGPVRILCAMPFFWIGGLLAATGALHEPVTVLVLPRLDAQTALDLIERERATGLVGWPAFTQRLRDHPSFAAR
DLSSAPMLRDGPLDIAMTDVPDGYPVHRTMSETAGGFAFTEMAIVDDEGQAVPEGTVGELLVRGIGVMAGYNKRERAETF
DADGWYHTGDRVYRREGDPRLFYVGRTSELIKAAGANVSPLEVEAVVEQFPDVVQCVVVGVEDPERGEQVCAAVVPARGE
IDVTDLSARARTQLSAYKVPTRWAVVGADQLPVLASGKLDRKAVKKMIADGVLPSVAPT
>Mature_539_residues
MDQPPARPRGGRCDPVGEDRQVIPPRPTIPALLARSAREFGDQAYVISPTERLTYGDAERRSAEVARWLLGRGVGKGVRV
GLFFPNGVDWIVWWLAVSRIGALAVPLSTMYTPAELAKVVRLADVALVVGPSAVLGIDVAQRFEEAFPGLADQPAGRLAL
TQAPYLRSVVLTGGADRPWVTPHDSSEATVSREVLAAVEDEVSPADLAVMVHTSGSTADPKGVLHTHGTVVRQTSGWPAA
VRAVTGSAGPVRILCAMPFFWIGGLLAATGALHEPVTVLVLPRLDAQTALDLIERERATGLVGWPAFTQRLRDHPSFAAR
DLSSAPMLRDGPLDIAMTDVPDGYPVHRTMSETAGGFAFTEMAIVDDEGQAVPEGTVGELLVRGIGVMAGYNKRERAETF
DADGWYHTGDRVYRREGDPRLFYVGRTSELIKAAGANVSPLEVEAVVEQFPDVVQCVVVGVEDPERGEQVCAAVVPARGE
IDVTDLSARARTQLSAYKVPTRWAVVGADQLPVLASGKLDRKAVKKMIADGVLPSVAPT

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=517, Percent_Identity=25.531914893617, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI187761345, Length=174, Percent_Identity=36.7816091954023, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI187761343, Length=174, Percent_Identity=36.7816091954023, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI58082049, Length=520, Percent_Identity=21.5384615384615, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI38505220, Length=517, Percent_Identity=23.21083172147, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI115511026, Length=511, Percent_Identity=21.7221135029354, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI157311624, Length=531, Percent_Identity=22.4105461393597, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI157311622, Length=531, Percent_Identity=22.4105461393597, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI42544132, Length=500, Percent_Identity=22.8, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788107, Length=573, Percent_Identity=26.7015706806283, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI221142682, Length=515, Percent_Identity=25.8252427184466, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=23.7827715355805, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786810, Length=174, Percent_Identity=31.6091954022989, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786801, Length=562, Percent_Identity=24.7330960854093, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788595, Length=338, Percent_Identity=25.4437869822485, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994694, Length=535, Percent_Identity=25.7943925233645, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71994703, Length=535, Percent_Identity=25.7943925233645, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71994690, Length=535, Percent_Identity=25.7943925233645, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17560308, Length=522, Percent_Identity=27.3946360153257, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=536, Percent_Identity=25.7462686567164, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=531, Percent_Identity=25.8003766478343, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=493, Percent_Identity=23.9350912778905, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=517, Percent_Identity=23.21083172147, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=491, Percent_Identity=25.2545824847251, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=363, Percent_Identity=24.7933884297521, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319699, Length=502, Percent_Identity=24.9003984063745, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581924, Length=537, Percent_Identity=26.4432029795158, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21355181, Length=526, Percent_Identity=23.9543726235741, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI18859661, Length=518, Percent_Identity=25.6756756756757, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21356441, Length=352, Percent_Identity=26.1363636363636, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21358303, Length=334, Percent_Identity=25.748502994012, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648253, Length=373, Percent_Identity=25.4691689008043, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648255, Length=373, Percent_Identity=25.7372654155496, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648260, Length=170, Percent_Identity=31.7647058823529, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24653035, Length=168, Percent_Identity=31.547619047619, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356947, Length=358, Percent_Identity=25.6983240223464, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648257, Length=172, Percent_Identity=34.3023255813954, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281365686, Length=169, Percent_Identity=27.810650887574, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=176, Percent_Identity=27.8409090909091, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57624; Mature: 57624

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQPPARPRGGRCDPVGEDRQVIPPRPTIPALLARSAREFGDQAYVISPTERLTYGDAER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCCHHH
RSAEVARWLLGRGVGKGVRVGLFFPNGVDWIVWWLAVSRIGALAVPLSTMYTPAELAKVV
HHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RLADVALVVGPSAVLGIDVAQRFEEAFPGLADQPAGRLALTQAPYLRSVVLTGGADRPWV
HHHHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHEEEEECCCCCCCC
TPHDSSEATVSREVLAAVEDEVSPADLAVMVHTSGSTADPKGVLHTHGTVVRQTSGWPAA
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEECCCEEEEECCCCCHH
VRAVTGSAGPVRILCAMPFFWIGGLLAATGALHEPVTVLVLPRLDAQTALDLIERERATG
HHEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCC
LVGWPAFTQRLRDHPSFAARDLSSAPMLRDGPLDIAMTDVPDGYPVHRTMSETAGGFAFT
EEECHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEE
EMAIVDDEGQAVPEGTVGELLVRGIGVMAGYNKRERAETFDADGWYHTGDRVYRREGDPR
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEECCCEEEEECCCCE
LFYVGRTSELIKAAGANVSPLEVEAVVEQFPDVVQCVVVGVEDPERGEQVCAAVVPARGE
EEEECCHHHHHHHCCCCCCCHHHHHHHHHCHHHEEEEEECCCCCCHHHHHHHHHCCCCCC
IDVTDLSARARTQLSAYKVPTRWAVVGADQLPVLASGKLDRKAVKKMIADGVLPSVAPT
EEEECCCHHHHHHHHHEECCCEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDQPPARPRGGRCDPVGEDRQVIPPRPTIPALLARSAREFGDQAYVISPTERLTYGDAER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCCHHH
RSAEVARWLLGRGVGKGVRVGLFFPNGVDWIVWWLAVSRIGALAVPLSTMYTPAELAKVV
HHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RLADVALVVGPSAVLGIDVAQRFEEAFPGLADQPAGRLALTQAPYLRSVVLTGGADRPWV
HHHHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHEEEEECCCCCCCC
TPHDSSEATVSREVLAAVEDEVSPADLAVMVHTSGSTADPKGVLHTHGTVVRQTSGWPAA
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEECCCEEEEECCCCCHH
VRAVTGSAGPVRILCAMPFFWIGGLLAATGALHEPVTVLVLPRLDAQTALDLIERERATG
HHEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCC
LVGWPAFTQRLRDHPSFAARDLSSAPMLRDGPLDIAMTDVPDGYPVHRTMSETAGGFAFT
EEECHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEE
EMAIVDDEGQAVPEGTVGELLVRGIGVMAGYNKRERAETFDADGWYHTGDRVYRREGDPR
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEECCCEEEEECCCCE
LFYVGRTSELIKAAGANVSPLEVEAVVEQFPDVVQCVVVGVEDPERGEQVCAAVVPARGE
EEEECCHHHHHHHCCCCCCCHHHHHHHHHCHHHEEEEEECCCCCCHHHHHHHHHCCCCCC
IDVTDLSARARTQLSAYKVPTRWAVVGADQLPVLASGKLDRKAVKKMIADGVLPSVAPT
EEEECCCHHHHHHHHHEECCCEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]