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The map label for this gene is alkJ [H]

Identifier: 119868705

GI number: 119868705

Start: 2803971

End: 2805665

Strand: Direct

Name: alkJ [H]

Synonym: Mkms_2672

Alternate gene names: 119868705

Gene position: 2803971-2805665 (Clockwise)

Preceding gene: 119868702

Following gene: 119868706

Centisome position: 48.87

GC content: 65.96

Gene sequence:

>1695_bases
ATGCCGGATATGAGCCCCGTCGCCGAGTTCGACTTCGTCATCGTGGGCGCAGGCAGTGCGGGCTGCCTGCTCGCCAACCG
GCTCAGCGCCAACCCGGATCACCGGGTGCTCTTGATCGAGGCCGGCGGCACAGACAACTGGTTCTGGATCAAGGTGCCAG
TGGGCTACCTGTACACAATTGCCAACCCCCGCACCGACTGGTGCTTCACGACCGAGGCCGACCCGGGTCTGGCCGGCCGC
AGCATTCACTACGCGCGGGGCCGTGTGATCGGCGGCAGCTCGTCGATCAACGCCATGATCCATATGCGCGGGCAAGCGTC
CGATTACGAGCTCTGGGCACAGGCCACCGGTGACGAGCGCTGGCTATGGGGCGGCCCGGACGGCGCCGGCGAGACGTTGT
CGATCTACAAGGATTTGGAGGACTACTTCGGCGGAGCCGACGAGTGGCACGGTGCCGGTGGCGAGATCCCCGTAGAGCGA
CCGCGGGTGCGCTGGAAGATCCTGGACGCCTGGCAGGCCGCCGCCGCCCAGGTGGGTATTGCTCCGATCGAGGAGTTCAA
CCGCGGAGACAACGCCGGCAGTGCGTACTTTCACGTGAACCAACGGCGTGGCCGTCGCTGGTCGATGGCGGATGCCTTCC
TGCATCCCGTCTCCCACCGACCAAATCTCACCGTCTATACCCAGACCCAGGCCTTACAGCTGCTGATGGACGGCCAGGTC
CACGACGCTCAGCGTCGCGGTGCCTGGACCACTGCTGCGCACCGTGCCACCGGCGTGCGGCTGCTCAAAGACGGTCGGAC
CATCGACGTTCGGGCCCGCCGGGAGGTGATCCTGAGCGCCGGAGCGATCGGGTCGCCGCATCTCATGCAGGCCTCGGGTC
TGGGCCCGGCCTCGCTGCTCACCCAGCATCAGGTGCCGGTGGCGGTCGATCTGCCCGGAGTGGGTGAAAACCTCCAGGAC
CACCTGCAGCTGCGAACGGTCTACCGGGTACGAGGCGCCCGGACCGTCAACACGCTGTACCGCAATTGGATCACCCGTGC
AGGCATGGGACTTCAGTACCTGCTGCTGCGATCAGGACCCATGACCATGCCGCCCTCCACGCTGGGCGCTTTCGCCAAAA
GCGACCCCGCACTGGCCAGTCCAGATCTGGAGTGGCATGTGCAGCCCTTGTCGTTGGCCAAGTTCGGCGAACCTCTGCAC
AAATTCGGGGCGATCACACCCTCGGTGTGCAATCTGCGACCCAGTTCGCGTGGCCACGTACGCATCACCAGTGCCGATCC
AATGACGTACCCGAAGATCGTCTGCAACTACCTGTCCACCGACGACGACCGTCGAATCGCCGTGCGGGGCTTGAGGATGA
CCCGGCAGATCATGGCGGCGCCGTCCCTGGCCCGCTACTGCCCAGAAGAAATGCTTCCTGGCCCGCAACTGGTGAGCGAC
GACGACCTGCAGCAGGCGGCTCGTGAGCTCGGTACCACGATCTTTCACCCGGTGGGCACCTGCGCGATGGGAGCCTTCGA
CACACGCGGTCTGCCGAGGTCGGCGACCACGGTGCTCGACACCGAGTGCCGGGTGTACCGCGTCGCCGGCCTTCGCGTGG
TCGATGCTTCGGCGATGCCCACCATCACCTCCGGCAACACCAACGCGCCGGTCATGATGATCGCAGAACGCGCAGCGCGG
GCGATCCTGGGATGA

Upstream 100 bases:

>100_bases
ACGTCGAGTTCTGCGACAACTGCAGGGCACGAAGTCTTAGTCTCTCCCGACGACGACACGGTGAACAGCGACAGCACCCA
CGGAACGCCATGGAGGAACG

Downstream 100 bases:

>100_bases
GTCGGTCGATTTCAAGACGCGCCGATCCGGAATAAACCCCGCACCGGTGAACTTGGCTCAGGAGTCGCCCCTTCCCCGAC
TCAGGAGCATTGATGACCCG

Product: glucose-methanol-choline oxidoreductase

Products: betaine aldehyde; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MPDMSPVAEFDFVIVGAGSAGCLLANRLSANPDHRVLLIEAGGTDNWFWIKVPVGYLYTIANPRTDWCFTTEADPGLAGR
SIHYARGRVIGGSSSINAMIHMRGQASDYELWAQATGDERWLWGGPDGAGETLSIYKDLEDYFGGADEWHGAGGEIPVER
PRVRWKILDAWQAAAAQVGIAPIEEFNRGDNAGSAYFHVNQRRGRRWSMADAFLHPVSHRPNLTVYTQTQALQLLMDGQV
HDAQRRGAWTTAAHRATGVRLLKDGRTIDVRARREVILSAGAIGSPHLMQASGLGPASLLTQHQVPVAVDLPGVGENLQD
HLQLRTVYRVRGARTVNTLYRNWITRAGMGLQYLLLRSGPMTMPPSTLGAFAKSDPALASPDLEWHVQPLSLAKFGEPLH
KFGAITPSVCNLRPSSRGHVRITSADPMTYPKIVCNYLSTDDDRRIAVRGLRMTRQIMAAPSLARYCPEEMLPGPQLVSD
DDLQQAARELGTTIFHPVGTCAMGAFDTRGLPRSATTVLDTECRVYRVAGLRVVDASAMPTITSGNTNAPVMMIAERAAR
AILG

Sequences:

>Translated_564_residues
MPDMSPVAEFDFVIVGAGSAGCLLANRLSANPDHRVLLIEAGGTDNWFWIKVPVGYLYTIANPRTDWCFTTEADPGLAGR
SIHYARGRVIGGSSSINAMIHMRGQASDYELWAQATGDERWLWGGPDGAGETLSIYKDLEDYFGGADEWHGAGGEIPVER
PRVRWKILDAWQAAAAQVGIAPIEEFNRGDNAGSAYFHVNQRRGRRWSMADAFLHPVSHRPNLTVYTQTQALQLLMDGQV
HDAQRRGAWTTAAHRATGVRLLKDGRTIDVRARREVILSAGAIGSPHLMQASGLGPASLLTQHQVPVAVDLPGVGENLQD
HLQLRTVYRVRGARTVNTLYRNWITRAGMGLQYLLLRSGPMTMPPSTLGAFAKSDPALASPDLEWHVQPLSLAKFGEPLH
KFGAITPSVCNLRPSSRGHVRITSADPMTYPKIVCNYLSTDDDRRIAVRGLRMTRQIMAAPSLARYCPEEMLPGPQLVSD
DDLQQAARELGTTIFHPVGTCAMGAFDTRGLPRSATTVLDTECRVYRVAGLRVVDASAMPTITSGNTNAPVMMIAERAAR
AILG
>Mature_563_residues
PDMSPVAEFDFVIVGAGSAGCLLANRLSANPDHRVLLIEAGGTDNWFWIKVPVGYLYTIANPRTDWCFTTEADPGLAGRS
IHYARGRVIGGSSSINAMIHMRGQASDYELWAQATGDERWLWGGPDGAGETLSIYKDLEDYFGGADEWHGAGGEIPVERP
RVRWKILDAWQAAAAQVGIAPIEEFNRGDNAGSAYFHVNQRRGRRWSMADAFLHPVSHRPNLTVYTQTQALQLLMDGQVH
DAQRRGAWTTAAHRATGVRLLKDGRTIDVRARREVILSAGAIGSPHLMQASGLGPASLLTQHQVPVAVDLPGVGENLQDH
LQLRTVYRVRGARTVNTLYRNWITRAGMGLQYLLLRSGPMTMPPSTLGAFAKSDPALASPDLEWHVQPLSLAKFGEPLHK
FGAITPSVCNLRPSSRGHVRITSADPMTYPKIVCNYLSTDDDRRIAVRGLRMTRQIMAAPSLARYCPEEMLPGPQLVSDD
DLQQAARELGTTIFHPVGTCAMGAFDTRGLPRSATTVLDTECRVYRVAGLRVVDASAMPTITSGNTNAPVMMIAERAARA
ILG

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=563, Percent_Identity=35.7015985790409, Blast_Score=327, Evalue=2e-89,
Organism=Escherichia coli, GI1786503, Length=566, Percent_Identity=35.5123674911661, Blast_Score=319, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17532301, Length=572, Percent_Identity=32.8671328671329, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI45549471, Length=591, Percent_Identity=33.502538071066, Blast_Score=269, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24642042, Length=592, Percent_Identity=33.7837837837838, Blast_Score=269, Evalue=5e-72,
Organism=Drosophila melanogaster, GI45551458, Length=591, Percent_Identity=33.3333333333333, Blast_Score=269, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24642048, Length=596, Percent_Identity=32.7181208053691, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24642055, Length=600, Percent_Identity=34, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24642059, Length=591, Percent_Identity=31.3028764805415, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24650267, Length=609, Percent_Identity=32.3481116584565, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI17137792, Length=583, Percent_Identity=31.5608919382504, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI18859995, Length=592, Percent_Identity=31.5878378378378, Blast_Score=218, Evalue=8e-57,
Organism=Drosophila melanogaster, GI24642039, Length=595, Percent_Identity=30.5882352941176, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24642051, Length=594, Percent_Identity=29.1245791245791, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24642035, Length=600, Percent_Identity=30.1666666666667, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24642037, Length=597, Percent_Identity=29.4807370184255, Blast_Score=205, Evalue=8e-53,
Organism=Drosophila melanogaster, GI18859993, Length=598, Percent_Identity=26.9230769230769, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24645930, Length=606, Percent_Identity=29.2079207920792, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24642057, Length=612, Percent_Identity=27.7777777777778, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1

Molecular weight: Translated: 61546; Mature: 61415

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDMSPVAEFDFVIVGAGSAGCLLANRLSANPDHRVLLIEAGGTDNWFWIKVPVGYLYTI
CCCCCCCCCCCEEEEECCCCCEEEEHHHCCCCCCEEEEEECCCCCCEEEEEEECCEEEEE
ANPRTDWCFTTEADPGLAGRSIHYARGRVIGGSSSINAMIHMRGQASDYELWAQATGDER
CCCCCCEEEEECCCCCCCCCEEEEECCEEECCCCCCEEEEEECCCCCCEEEEEECCCCCE
WLWGGPDGAGETLSIYKDLEDYFGGADEWHGAGGEIPVERPRVRWKILDAWQAAAAQVGI
EEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCC
APIEEFNRGDNAGSAYFHVNQRRGRRWSMADAFLHPVSHRPNLTVYTQTQALQLLMDGQV
CCHHHHCCCCCCCCEEEEEECCCCCEECHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCC
HDAQRRGAWTTAAHRATGVRLLKDGRTIDVRARREVILSAGAIGSPHLMQASGLGPASLL
HHHHHCCCCHHHHHHHCCEEEEECCCEEEEEHHEEEEEECCCCCCCCEEEECCCCHHHHH
TQHQVPVAVDLPGVGENLQDHLQLRTVYRVRGARTVNTLYRNWITRAGMGLQYLLLRSGP
HCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCC
MTMPPSTLGAFAKSDPALASPDLEWHVQPLSLAKFGEPLHKFGAITPSVCNLRPSSRGHV
CCCCCHHHHHHHCCCCCCCCCCCEEEECCHHHHHHCCHHHHHCCCCCHHHCCCCCCCCCE
RITSADPMTYPKIVCNYLSTDDDRRIAVRGLRMTRQIMAAPSLARYCPEEMLPGPQLVSD
EEECCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCHHHHHCCHHHCCCCCCCCC
DDLQQAARELGTTIFHPVGTCAMGAFDTRGLPRSATTVLDTECRVYRVAGLRVVDASAMP
HHHHHHHHHHCCHHHCCHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEECEEEEECCCCC
TITSGNTNAPVMMIAERAARAILG
EEECCCCCCCEEEEEHHHHHHHCC
>Mature Secondary Structure 
PDMSPVAEFDFVIVGAGSAGCLLANRLSANPDHRVLLIEAGGTDNWFWIKVPVGYLYTI
CCCCCCCCCCEEEEECCCCCEEEEHHHCCCCCCEEEEEECCCCCCEEEEEEECCEEEEE
ANPRTDWCFTTEADPGLAGRSIHYARGRVIGGSSSINAMIHMRGQASDYELWAQATGDER
CCCCCCEEEEECCCCCCCCCEEEEECCEEECCCCCCEEEEEECCCCCCEEEEEECCCCCE
WLWGGPDGAGETLSIYKDLEDYFGGADEWHGAGGEIPVERPRVRWKILDAWQAAAAQVGI
EEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCC
APIEEFNRGDNAGSAYFHVNQRRGRRWSMADAFLHPVSHRPNLTVYTQTQALQLLMDGQV
CCHHHHCCCCCCCCEEEEEECCCCCEECHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCC
HDAQRRGAWTTAAHRATGVRLLKDGRTIDVRARREVILSAGAIGSPHLMQASGLGPASLL
HHHHHCCCCHHHHHHHCCEEEEECCCEEEEEHHEEEEEECCCCCCCCEEEECCCCHHHHH
TQHQVPVAVDLPGVGENLQDHLQLRTVYRVRGARTVNTLYRNWITRAGMGLQYLLLRSGP
HCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCC
MTMPPSTLGAFAKSDPALASPDLEWHVQPLSLAKFGEPLHKFGAITPSVCNLRPSSRGHV
CCCCCHHHHHHHCCCCCCCCCCCEEEECCHHHHHHCCHHHHHCCCCCHHHCCCCCCCCCE
RITSADPMTYPKIVCNYLSTDDDRRIAVRGLRMTRQIMAAPSLARYCPEEMLPGPQLVSD
EEECCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCHHHHHCCHHHCCCCCCCCC
DDLQQAARELGTTIFHPVGTCAMGAFDTRGLPRSATTVLDTECRVYRVAGLRVVDASAMP
HHHHHHHHHHCCHHHCCHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEECEEEEECCCCC
TITSGNTNAPVMMIAERAARAILG
EEECCCCCCCEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: choline; acceptor

Specific reaction: choline + acceptor = betaine aldehyde + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]