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The map label for this gene is hpaG [H]

Identifier: 119868692

GI number: 119868692

Start: 2790870

End: 2792399

Strand: Reverse

Name: hpaG [H]

Synonym: Mkms_2659

Alternate gene names: 119868692

Gene position: 2792399-2790870 (Counterclockwise)

Preceding gene: 119868699

Following gene: 119868691

Centisome position: 48.67

GC content: 67.25

Gene sequence:

>1530_bases
GTGACCATCGAACCCGACAGCGCCAAAGTGTCTACCGGCGTGGCCATTTCGGTCGCCGAGGTGATCGAGCGCAGTCGCAA
GGTGATCGCCGTGCATCTGAACTACCGCTCACGTGCCGCCCAGCGGGGCCGTCTCCCGGGTCATCCGTCGTATTTCCTCA
AGCCGCCGACGTCGCTGGCGTTGAGCGGACGACCGGTGGAGCGGCCGGCGGGAACGAGGTTGCTGGCTTTCGAGGGCGAG
ATCGCGCTCGTCATCGGCCGTCGCACGCGGCGGGTGTCACCGGAGAATTCCTGGGATTGTGTTCAGTTCGTCTGTGCAGC
CAATGATCTCGGCGTCTACGACCTCCGCGCAGTGGACAAGGGGTCGAACCTCCGGTCCAAAGGCGGTGACGGATTCACCC
CGATCGGGCCGCAGTTACTGAGTGCACGAGGAATCGATCCCGCTCGGCTTCGGGTCCGAACCTGGGTGAACGGACGACTC
GCACAGGATGGCGACACCTCGGACCTGCTTTTCCCGTTCACCCAGTTGATCGCCGACCTGTCCCAGTTGATCACTCTCGA
AGCGGGTGACGTCATCTTGACCGGCACCCCCGCCGGCTCCAGCGTGGTCGCCCCCGGAGACACGGTGGAGGTCCAGGTCG
ACAGTGTCGAGGACCCGCGCCTGAGTACCGGCCGGCTGTGCACACCGGTCGTTGCCGGTACCGACGCGTTCGCCGACTTC
GGTGCCCAACCGGACGCCGACGAAGCGCAGATCGTCGATGCCTGGGGGAGCCGCGTCGCGGCCGGTCTGGAACCGGAGTT
CGTCCTGACCGAGTCGCTGAAGGCGAAGCTGGCATCGGTGGGAACAGCCACCTTGTCCGCGCAGTTACGTAAGCGCGGCC
TCAACACGGTCACCATCGACGGACTCACGCCGACCCATCCGGACAAACGCATGGTGGGAAGGGCCCGGACCTTGCGGTTC
GTGCCGCTGCGCGAGGACCTCTTCAAAGCCCGAGGCGGCGGCTACAACGCACAGAAGCGGGCCTTCGACAGCCTGGAGCC
CGGCGATGTTCTGGTGATCGAGGGCCGGGGCGAAAAGGGCAGCGGCACCGTCGGCGACATCCTCGCGTTGCGCGCCCAGG
TGCGCGGGGCGGTCGGCATCGTCACCGACGGCGGCGTGCGCGATGTGGCCGCTGTTTCGGCGCTGGACATCTGCACATTC
CACGGCGGCCCGCACCCCGCCGTGCTGGGCCGCAGACACGTGCCCTGGGATGTGGACATCGCGGTGGCCTGCGGCGGCGC
GACGGTGGAGCCGGGCGACGTCCTGGTCGGCGACGCCGACGGAGTCGTGGTGATTCCACCGGGTCTCGCCGAGGACATCG
CCGACTCGGCAATAGCCCAGGAGACCGAAGAGCAGTACATCGCCGACCGGGTCGCCGAAGGTGCCGCACTCGAAGGTCTC
TATCCCATGAACGACTCGGCACGCGCCGACTACGAGCAGTCTCGGCACGGCCGCCACGCCGACACCACCCCGAAAGGTTC
GACACCATGA

Upstream 100 bases:

>100_bases
CATCCAGGTCACACCCGATTGCGGAAAACTGAAAAAACAGGTGATCATCAGCGCTTTTCAGCGCTTTACCGGCCGCGCAG
GGTCAACGCATGATGCTGGT

Downstream 100 bases:

>100_bases
CAGCCCGTCGAGATCCGCACAGCATCCGCGGATCCATCACTCCGTTGATGACGCCGTTCACCGACGACGGCGCAGTCGAT
CATCGGAGCCTTGCCAATCT

Product: hypothetical protein

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTIEPDSAKVSTGVAISVAEVIERSRKVIAVHLNYRSRAAQRGRLPGHPSYFLKPPTSLALSGRPVERPAGTRLLAFEGE
IALVIGRRTRRVSPENSWDCVQFVCAANDLGVYDLRAVDKGSNLRSKGGDGFTPIGPQLLSARGIDPARLRVRTWVNGRL
AQDGDTSDLLFPFTQLIADLSQLITLEAGDVILTGTPAGSSVVAPGDTVEVQVDSVEDPRLSTGRLCTPVVAGTDAFADF
GAQPDADEAQIVDAWGSRVAAGLEPEFVLTESLKAKLASVGTATLSAQLRKRGLNTVTIDGLTPTHPDKRMVGRARTLRF
VPLREDLFKARGGGYNAQKRAFDSLEPGDVLVIEGRGEKGSGTVGDILALRAQVRGAVGIVTDGGVRDVAAVSALDICTF
HGGPHPAVLGRRHVPWDVDIAVACGGATVEPGDVLVGDADGVVVIPPGLAEDIADSAIAQETEEQYIADRVAEGAALEGL
YPMNDSARADYEQSRHGRHADTTPKGSTP

Sequences:

>Translated_509_residues
MTIEPDSAKVSTGVAISVAEVIERSRKVIAVHLNYRSRAAQRGRLPGHPSYFLKPPTSLALSGRPVERPAGTRLLAFEGE
IALVIGRRTRRVSPENSWDCVQFVCAANDLGVYDLRAVDKGSNLRSKGGDGFTPIGPQLLSARGIDPARLRVRTWVNGRL
AQDGDTSDLLFPFTQLIADLSQLITLEAGDVILTGTPAGSSVVAPGDTVEVQVDSVEDPRLSTGRLCTPVVAGTDAFADF
GAQPDADEAQIVDAWGSRVAAGLEPEFVLTESLKAKLASVGTATLSAQLRKRGLNTVTIDGLTPTHPDKRMVGRARTLRF
VPLREDLFKARGGGYNAQKRAFDSLEPGDVLVIEGRGEKGSGTVGDILALRAQVRGAVGIVTDGGVRDVAAVSALDICTF
HGGPHPAVLGRRHVPWDVDIAVACGGATVEPGDVLVGDADGVVVIPPGLAEDIADSAIAQETEEQYIADRVAEGAALEGL
YPMNDSARADYEQSRHGRHADTTPKGSTP
>Mature_508_residues
TIEPDSAKVSTGVAISVAEVIERSRKVIAVHLNYRSRAAQRGRLPGHPSYFLKPPTSLALSGRPVERPAGTRLLAFEGEI
ALVIGRRTRRVSPENSWDCVQFVCAANDLGVYDLRAVDKGSNLRSKGGDGFTPIGPQLLSARGIDPARLRVRTWVNGRLA
QDGDTSDLLFPFTQLIADLSQLITLEAGDVILTGTPAGSSVVAPGDTVEVQVDSVEDPRLSTGRLCTPVVAGTDAFADFG
AQPDADEAQIVDAWGSRVAAGLEPEFVLTESLKAKLASVGTATLSAQLRKRGLNTVTIDGLTPTHPDKRMVGRARTLRFV
PLREDLFKARGGGYNAQKRAFDSLEPGDVLVIEGRGEKGSGTVGDILALRAQVRGAVGIVTDGGVRDVAAVSALDICTFH
GGPHPAVLGRRHVPWDVDIAVACGGATVEPGDVLVGDADGVVVIPPGLAEDIADSAIAQETEEQYIADRVAEGAALEGLY
PMNDSARADYEQSRHGRHADTTPKGSTP

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0684

COG function: function code H; Demethylmenaquinone methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI215422413, Length=208, Percent_Identity=32.6923076923077, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI13654274, Length=192, Percent_Identity=33.8541666666667, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI66348062, Length=192, Percent_Identity=33.8541666666667, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI156231349, Length=205, Percent_Identity=30.2439024390244, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI40786394, Length=200, Percent_Identity=30, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1787428, Length=193, Percent_Identity=31.0880829015544, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17557057, Length=198, Percent_Identity=30.3030303030303, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324161, Length=228, Percent_Identity=30.7017543859649, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571789, Length=199, Percent_Identity=33.6683417085427, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI28572127, Length=216, Percent_Identity=31.4814814814815, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24663695, Length=219, Percent_Identity=30.1369863013699, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 53674; Mature: 53543

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEPDSAKVSTGVAISVAEVIERSRKVIAVHLNYRSRAAQRGRLPGHPSYFLKPPTSLA
CCCCCCCCEECCCEEEEHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCCEEECCCCCEE
LSGRPVERPAGTRLLAFEGEIALVIGRRTRRVSPENSWDCVQFVCAANDLGVYDLRAVDK
ECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHEECCCCCCCEEEEEECC
GSNLRSKGGDGFTPIGPQLLSARGIDPARLRVRTWVNGRLAQDGDTSDLLFPFTQLIADL
CCCCCCCCCCCCCCCCHHHHHHCCCCHHHEEEEEEECCEECCCCCCCHHHHHHHHHHHHH
SQLITLEAGDVILTGTPAGSSVVAPGDTVEVQVDSVEDPRLSTGRLCTPVVAGTDAFADF
HHHEEEECCCEEEECCCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCHHHHHC
GAQPDADEAQIVDAWGSRVAAGLEPEFVLTESLKAKLASVGTATLSAQLRKRGLNTVTID
CCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEC
GLTPTHPDKRMVGRARTLRFVPLREDLFKARGGGYNAQKRAFDSLEPGDVLVIEGRGEKG
CCCCCCCCHHHHHHHHEEEEEECHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECCCCCC
SGTVGDILALRAQVRGAVGIVTDGGVRDVAAVSALDICTFHGGPHPAVLGRRHVPWDVDI
CCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHEEEECCCCCCHHCCCCCCCCCEEE
AVACGGATVEPGDVLVGDADGVVVIPPGLAEDIADSAIAQETEEQYIADRVAEGAALEGL
EEEECCCEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
YPMNDSARADYEQSRHGRHADTTPKGSTP
CCCCCCCCCCHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIEPDSAKVSTGVAISVAEVIERSRKVIAVHLNYRSRAAQRGRLPGHPSYFLKPPTSLA
CCCCCCCEECCCEEEEHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCCEEECCCCCEE
LSGRPVERPAGTRLLAFEGEIALVIGRRTRRVSPENSWDCVQFVCAANDLGVYDLRAVDK
ECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHEECCCCCCCEEEEEECC
GSNLRSKGGDGFTPIGPQLLSARGIDPARLRVRTWVNGRLAQDGDTSDLLFPFTQLIADL
CCCCCCCCCCCCCCCCHHHHHHCCCCHHHEEEEEEECCEECCCCCCCHHHHHHHHHHHHH
SQLITLEAGDVILTGTPAGSSVVAPGDTVEVQVDSVEDPRLSTGRLCTPVVAGTDAFADF
HHHEEEECCCEEEECCCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCHHHHHC
GAQPDADEAQIVDAWGSRVAAGLEPEFVLTESLKAKLASVGTATLSAQLRKRGLNTVTID
CCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEC
GLTPTHPDKRMVGRARTLRFVPLREDLFKARGGGYNAQKRAFDSLEPGDVLVIEGRGEKG
CCCCCCCCHHHHHHHHEEEEEECHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECCCCCC
SGTVGDILALRAQVRGAVGIVTDGGVRDVAAVSALDICTFHGGPHPAVLGRRHVPWDVDI
CCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHEEEECCCCCCHHCCCCCCCCCEEE
AVACGGATVEPGDVLVGDADGVVVIPPGLAEDIADSAIAQETEEQYIADRVAEGAALEGL
EEEECCCEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
YPMNDSARADYEQSRHGRHADTTPKGSTP
CCCCCCCCCCHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA