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The map label for this gene is leuB [H]

Identifier: 119868652

GI number: 119868652

Start: 2751106

End: 2752188

Strand: Reverse

Name: leuB [H]

Synonym: Mkms_2617

Alternate gene names: 119868652

Gene position: 2752188-2751106 (Counterclockwise)

Preceding gene: 119868653

Following gene: 119868649

Centisome position: 47.97

GC content: 70.18

Gene sequence:

>1083_bases
ATGGCGACAGAGGATCCGTTGCGGCTGGGAGTGGTGGCCGGCGACGGTATCGGACCGGAGATCGTCTCGGCCGCAGTGCA
GATCAGCGAGCGGGCGTTGGCCGCGGTGTCGGTCCCCGTCGACTGGCGGCGGCTGCGCATGGGCGCCGAGGCCATCACCG
CGGAGGGCACTCCCCTGCCGGAAGCCACGCTGGCCGAACTCGACCGCCTCGACGCGTGGCTGCTCGGCCCGCACGACAAC
GCGGGCTATCCGGCGCCGTTTCGCGACGCGCTGTCCCCCGGCGGCACCATCCGCAAACGCTACGGGCTCTACGCCAACAT
CCGTCCGGCCCGCCGGTTGAGCGGCGCCATCCCCGCGGTCTGTCCCACCCTCGACGTCGTCGTGGTCCGCGAGAACACCG
AGGGCTTCTACTCCGACCGCAACATGTACGACGGCCCAGGCGAGTTCATGCCGACCGCCGACGTCGCGATGGCGGTCGCC
GTCTTCACCCGAGAAGCCTGTGAACGCATTGCGCACCAGGCGTTTCGGCTCGCGCGCACGCGGCGCCGCTCGGTCACCGT
CGCCCATAAGGCCAACGTGTTGTCCAAGACCACCGGCCTGTTCCGCGACAGTTGCGTGCGGGTGGCCGAACAATACCCCG
ACGTGACCGTCCGCCTCGAACACATCGACGCGCTCGCCGCCCTGCTGGTCGCGCGTCCCGCCGAATTCGACGTGATCGTG
GCGGAGAACATGTTCGGCGACATCCTGTCCGACCTCACCGGCCAGCTCGCCGGGTCACTGGGGATGGCGCCGTCGGTGAA
CCTGTCCGACACCAAGGCGATGGCCCAGGCCGCCCACGGCTCCGCACCGGACATCGCCGGGCACAACATCGCCAACCCGG
TCGCGATGATCCTGTCCACGGCGATGCTGCTCGAGTGGCTGGCAGAGCGACACCATCGTGGCGCCCTGGCCGGTGCCGCG
ACCCGGATACGGGATGCGGTGTGCCGCAGCCTCGATGACGGTGTGCACACCCCCGACCTCGGCGGGGCCGCGACCACCAC
CGGCTTCACCGAACACGTGCTCTCGGTGCTCGCCTCGGCGTGA

Upstream 100 bases:

>100_bases
CCGACGTCACCGACGCGCTGGTCGCCCAGTTGCGCTGACGAGCCGTCGACACCGGTACGGCACACTGAAGGAACCCGAAC
AGGAAGGTGCCGGTACCGAT

Downstream 100 bases:

>100_bases
CCGCTACGTCGCCTCGATCCCGTCTTTGCCGTCGGCGGCCTTCTCACCGGCGTCCTCGGTGTCGCGGCGCCGCTCCCGCA
ACACGAACGCCGCCAGCGCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MATEDPLRLGVVAGDGIGPEIVSAAVQISERALAAVSVPVDWRRLRMGAEAITAEGTPLPEATLAELDRLDAWLLGPHDN
AGYPAPFRDALSPGGTIRKRYGLYANIRPARRLSGAIPAVCPTLDVVVVRENTEGFYSDRNMYDGPGEFMPTADVAMAVA
VFTREACERIAHQAFRLARTRRRSVTVAHKANVLSKTTGLFRDSCVRVAEQYPDVTVRLEHIDALAALLVARPAEFDVIV
AENMFGDILSDLTGQLAGSLGMAPSVNLSDTKAMAQAAHGSAPDIAGHNIANPVAMILSTAMLLEWLAERHHRGALAGAA
TRIRDAVCRSLDDGVHTPDLGGAATTTGFTEHVLSVLASA

Sequences:

>Translated_360_residues
MATEDPLRLGVVAGDGIGPEIVSAAVQISERALAAVSVPVDWRRLRMGAEAITAEGTPLPEATLAELDRLDAWLLGPHDN
AGYPAPFRDALSPGGTIRKRYGLYANIRPARRLSGAIPAVCPTLDVVVVRENTEGFYSDRNMYDGPGEFMPTADVAMAVA
VFTREACERIAHQAFRLARTRRRSVTVAHKANVLSKTTGLFRDSCVRVAEQYPDVTVRLEHIDALAALLVARPAEFDVIV
AENMFGDILSDLTGQLAGSLGMAPSVNLSDTKAMAQAAHGSAPDIAGHNIANPVAMILSTAMLLEWLAERHHRGALAGAA
TRIRDAVCRSLDDGVHTPDLGGAATTTGFTEHVLSVLASA
>Mature_359_residues
ATEDPLRLGVVAGDGIGPEIVSAAVQISERALAAVSVPVDWRRLRMGAEAITAEGTPLPEATLAELDRLDAWLLGPHDNA
GYPAPFRDALSPGGTIRKRYGLYANIRPARRLSGAIPAVCPTLDVVVVRENTEGFYSDRNMYDGPGEFMPTADVAMAVAV
FTREACERIAHQAFRLARTRRRSVTVAHKANVLSKTTGLFRDSCVRVAEQYPDVTVRLEHIDALAALLVARPAEFDVIVA
ENMFGDILSDLTGQLAGSLGMAPSVNLSDTKAMAQAAHGSAPDIAGHNIANPVAMILSTAMLLEWLAERHHRGALAGAAT
RIRDAVCRSLDDGVHTPDLGGAATTTGFTEHVLSVLASA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=351, Percent_Identity=35.6125356125356, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI4758582, Length=348, Percent_Identity=32.4712643678161, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI28178838, Length=326, Percent_Identity=31.9018404907975, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI28178816, Length=359, Percent_Identity=30.9192200557103, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI28178821, Length=350, Percent_Identity=31.1428571428571, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI28178819, Length=208, Percent_Identity=35.0961538461538, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI87081683, Length=360, Percent_Identity=36.9444444444444, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788101, Length=366, Percent_Identity=33.879781420765, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1787381, Length=346, Percent_Identity=26.3005780346821, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71986051, Length=368, Percent_Identity=34.5108695652174, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25144293, Length=355, Percent_Identity=30.7042253521127, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17550882, Length=351, Percent_Identity=30.1994301994302, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17505779, Length=336, Percent_Identity=28.8690476190476, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6322097, Length=374, Percent_Identity=35.2941176470588, Blast_Score=189, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6324709, Length=357, Percent_Identity=35.5742296918768, Blast_Score=188, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=32.8611898016997, Blast_Score=169, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6319830, Length=381, Percent_Identity=33.0708661417323, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24643268, Length=363, Percent_Identity=34.7107438016529, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24643270, Length=363, Percent_Identity=34.7107438016529, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24661184, Length=372, Percent_Identity=30.9139784946237, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI161078635, Length=353, Percent_Identity=30.5949008498584, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI161078637, Length=353, Percent_Identity=30.5949008498584, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161078633, Length=353, Percent_Identity=30.5949008498584, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24650122, Length=353, Percent_Identity=30.5949008498584, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161078639, Length=353, Percent_Identity=30.8781869688385, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI281362242, Length=351, Percent_Identity=30.1994301994302, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24648872, Length=351, Percent_Identity=30.1994301994302, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI20130355, Length=353, Percent_Identity=25.2124645892351, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38229; Mature: 38098

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEDPLRLGVVAGDGIGPEIVSAAVQISERALAAVSVPVDWRRLRMGAEAITAEGTPLP
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHCHHHHCCCCCCCC
EATLAELDRLDAWLLGPHDNAGYPAPFRDALSPGGTIRKRYGLYANIRPARRLSGAIPAV
HHHHHHHHHCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHCCEECCCHHHHHCCCCCCC
CPTLDVVVVRENTEGFYSDRNMYDGPGEFMPTADVAMAVAVFTREACERIAHQAFRLART
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRRSVTVAHKANVLSKTTGLFRDSCVRVAEQYPDVTVRLEHIDALAALLVARPAEFDVIV
HHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCEEEE
AENMFGDILSDLTGQLAGSLGMAPSVNLSDTKAMAQAAHGSAPDIAGHNIANPVAMILST
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
AMLLEWLAERHHRGALAGAATRIRDAVCRSLDDGVHTPDLGGAATTTGFTEHVLSVLASA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ATEDPLRLGVVAGDGIGPEIVSAAVQISERALAAVSVPVDWRRLRMGAEAITAEGTPLP
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHCHHHHCCCCCCCC
EATLAELDRLDAWLLGPHDNAGYPAPFRDALSPGGTIRKRYGLYANIRPARRLSGAIPAV
HHHHHHHHHCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHCCEECCCHHHHHCCCCCCC
CPTLDVVVVRENTEGFYSDRNMYDGPGEFMPTADVAMAVAVFTREACERIAHQAFRLART
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRRSVTVAHKANVLSKTTGLFRDSCVRVAEQYPDVTVRLEHIDALAALLVARPAEFDVIV
HHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCEEEE
AENMFGDILSDLTGQLAGSLGMAPSVNLSDTKAMAQAAHGSAPDIAGHNIANPVAMILST
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
AMLLEWLAERHHRGALAGAATRIRDAVCRSLDDGVHTPDLGGAATTTGFTEHVLSVLASA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]