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The map label for this gene is bznB [H]

Identifier: 119868376

GI number: 119868376

Start: 2452071

End: 2453783

Strand: Reverse

Name: bznB [H]

Synonym: Mkms_2340

Alternate gene names: 119868376

Gene position: 2453783-2452071 (Counterclockwise)

Preceding gene: 119868378

Following gene: 119868374

Centisome position: 42.77

GC content: 71.4

Gene sequence:

>1713_bases
ATGACTGTCAGCGGCGGCGATCTACTCGCCCGGGCGTTGGCGGATGCGGGCGTCACGGAGGTGTTCACCCTGCACGGTGG
CCACCTGGACGCCTTCCTGGTGGCGTGCGGCAAACACGATGTCCGGCTCACCGATACGCGGCACGAGTCCAGCGCCGGCC
ACGCGGCGGACGCCTACGCGCGCGTGACCGGGAGTCTGGGGGTGTGCGTGGTGACCTCGGGTCCGGGCTTCACCAACGCC
TACACCGCGATCGCCAACGGCTACCTCGACCGCACCCCCACGCTGTTCATCGTGGGCGGGCCCCCGTTGCGGGAGAGCGC
GACCAACCCGCTGCAGGGCGGCTTCGATCAGGTGGCCGCGGCGGCGCCGGTGACCAAGTGGGCGTACCGGCTCACCGAAG
CCGCCCGCATTCCCGAGATCGTGTCCCTGGCCGTGCGTAAGGCGACCACCGGCGCACCGGGTCCGGTCCTGCTGGAGGTG
CCCATCGACGTGATGTTCGGCGAGGCGGACGAAGCCGAGGTGAGGTACCCGGCGCGCTACGCCCTCGACGCGCGTTCGGT
GCCGTCGGATTCCGTTGTCCACCAAGCGCTCGACGTGCTCCAGGGCGCCCAGCACCCGGTCATCGTCATCGGTGGTGGTG
TCACCTTCTCGCGCGCAGCCGACGCGCTGGTCGCCTTCGCCGAGACGGTCGGCGTTCCGGTGTTCTACCCCGGCAAGTCC
GACGGCGCCATCCCCGCCAGTCACCGACTCGCCGCGGGCGGACTGCTCGGGCTGGGCGCCCTGTCCGCGCTGGGCGCCCC
GACCCCCGATGTGGTGCTGCTCGTCGGCGCCCGGGCCGGCATGTTCACCGGAGGGCGCGCGAGCATGTTCCCCGGCGCCC
GCATCGTGCAGGTCGACCTGGACGCGGCCGAGATCGGCCGCATGCAGGATGTCGCCGTCGCGATAGTGGCCGACTGCCGG
GAGACGCTGCGGGCGTTGCAGGCCGCGGCGGCGGACCGCGACTGGCCGGACTGGTCTGAGTGGGTTCACACCGCGGTGCA
GGCGCAGCGGTTCCACCGCGCCCTCTACCCCGACGCTGCGACGCCCAGCGGTCGGATGCATCCGTACTTCGCCGCCCGCG
CCGTCGTCGAGGCATGCCCTCCGGGAACGACCTTCGTGCTCGACGGCGCCGAGGCGCCGGCCTGGGCGGAGTTCTTCGCG
ACCACCGAGACGGTCGGTGGTGTGCTGCGCCTGGGCTATCTCGGAACCCTCGGCGTCGGGCCGGGTTTCGCGATCGGTGC
CGCACGGGCCCGGCCGGCGGCCCCGGTGGTGCTCATCACCGGCGACGGTGCCGCCGGCTTCCACCTCGGCGAGTTCGACA
CCATGGCCCGCCACGGTATGCCGGTGTTGACGGTCGTCTTCAACAACGCGGTGTGGGGCATGTCGGTGCACGGCCAGCAG
GCCGTCTACGGGGACGGCGGTGTCGTGGTGTCCGAATTGGCCGACAGCTCATACGAAAAGGTCGCCGAGGCGTTCGGCGG
CTACGGTGAGCGCGTCGGCGAGGTCGACGAGATCGCCGCCGCCGTCCAGCGCGCATTCGCCGCGCAGGTTCCCGCCTGTC
TCAACCTCGAGATCGATCCGCACACCGTGCATCCACTGACCACGATGATGCTCGGCGACGTGACCGCCACCGACGCGATC
GTCGTGCCCTACTACGAGAACCTGCCCCGCTGA

Upstream 100 bases:

>100_bases
ACGTGTTCGCACTATGCCACCATTGTGACGCGTTGTGAAAACACTTATCGTGCCAACAAGCGACCACCTGCGATGTGTGG
TCGGGGCTTGGAGGTGGCAC

Downstream 100 bases:

>100_bases
CGAATCACCAGGCGCGCAGTGCGCACTGCGTGCCGCTGCCGCCGTCGGACTGCACGACGACCACACTGTCGACGGCGATC
TCGCAGTGGAAGTTCGGCGG

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: Benzoin aldolase; BL; BZL [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MTVSGGDLLARALADAGVTEVFTLHGGHLDAFLVACGKHDVRLTDTRHESSAGHAADAYARVTGSLGVCVVTSGPGFTNA
YTAIANGYLDRTPTLFIVGGPPLRESATNPLQGGFDQVAAAAPVTKWAYRLTEAARIPEIVSLAVRKATTGAPGPVLLEV
PIDVMFGEADEAEVRYPARYALDARSVPSDSVVHQALDVLQGAQHPVIVIGGGVTFSRAADALVAFAETVGVPVFYPGKS
DGAIPASHRLAAGGLLGLGALSALGAPTPDVVLLVGARAGMFTGGRASMFPGARIVQVDLDAAEIGRMQDVAVAIVADCR
ETLRALQAAAADRDWPDWSEWVHTAVQAQRFHRALYPDAATPSGRMHPYFAARAVVEACPPGTTFVLDGAEAPAWAEFFA
TTETVGGVLRLGYLGTLGVGPGFAIGAARARPAAPVVLITGDGAAGFHLGEFDTMARHGMPVLTVVFNNAVWGMSVHGQQ
AVYGDGGVVVSELADSSYEKVAEAFGGYGERVGEVDEIAAAVQRAFAAQVPACLNLEIDPHTVHPLTTMMLGDVTATDAI
VVPYYENLPR

Sequences:

>Translated_570_residues
MTVSGGDLLARALADAGVTEVFTLHGGHLDAFLVACGKHDVRLTDTRHESSAGHAADAYARVTGSLGVCVVTSGPGFTNA
YTAIANGYLDRTPTLFIVGGPPLRESATNPLQGGFDQVAAAAPVTKWAYRLTEAARIPEIVSLAVRKATTGAPGPVLLEV
PIDVMFGEADEAEVRYPARYALDARSVPSDSVVHQALDVLQGAQHPVIVIGGGVTFSRAADALVAFAETVGVPVFYPGKS
DGAIPASHRLAAGGLLGLGALSALGAPTPDVVLLVGARAGMFTGGRASMFPGARIVQVDLDAAEIGRMQDVAVAIVADCR
ETLRALQAAAADRDWPDWSEWVHTAVQAQRFHRALYPDAATPSGRMHPYFAARAVVEACPPGTTFVLDGAEAPAWAEFFA
TTETVGGVLRLGYLGTLGVGPGFAIGAARARPAAPVVLITGDGAAGFHLGEFDTMARHGMPVLTVVFNNAVWGMSVHGQQ
AVYGDGGVVVSELADSSYEKVAEAFGGYGERVGEVDEIAAAVQRAFAAQVPACLNLEIDPHTVHPLTTMMLGDVTATDAI
VVPYYENLPR
>Mature_569_residues
TVSGGDLLARALADAGVTEVFTLHGGHLDAFLVACGKHDVRLTDTRHESSAGHAADAYARVTGSLGVCVVTSGPGFTNAY
TAIANGYLDRTPTLFIVGGPPLRESATNPLQGGFDQVAAAAPVTKWAYRLTEAARIPEIVSLAVRKATTGAPGPVLLEVP
IDVMFGEADEAEVRYPARYALDARSVPSDSVVHQALDVLQGAQHPVIVIGGGVTFSRAADALVAFAETVGVPVFYPGKSD
GAIPASHRLAAGGLLGLGALSALGAPTPDVVLLVGARAGMFTGGRASMFPGARIVQVDLDAAEIGRMQDVAVAIVADCRE
TLRALQAAAADRDWPDWSEWVHTAVQAQRFHRALYPDAATPSGRMHPYFAARAVVEACPPGTTFVLDGAEAPAWAEFFAT
TETVGGVLRLGYLGTLGVGPGFAIGAARARPAAPVVLITGDGAAGFHLGEFDTMARHGMPVLTVVFNNAVWGMSVHGQQA
VYGDGGVVVSELADSSYEKVAEAFGGYGERVGEVDEIAAAVQRAFAAQVPACLNLEIDPHTVHPLTTMMLGDVTATDAIV
VPYYENLPR

Specific function: Cleavage of benzoin-anisoin acyloin linkage [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=552, Percent_Identity=29.7101449275362, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI93004078, Length=556, Percent_Identity=27.5179856115108, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI87081685, Length=549, Percent_Identity=29.5081967213115, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=28.1138790035587, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788716, Length=526, Percent_Identity=29.277566539924, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI1786717, Length=539, Percent_Identity=31.1688311688312, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1787096, Length=539, Percent_Identity=26.1595547309833, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=553, Percent_Identity=28.0289330922242, Blast_Score=209, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17531301, Length=544, Percent_Identity=28.125, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=544, Percent_Identity=28.125, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=26.056338028169, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320816, Length=573, Percent_Identity=24.0837696335079, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922626, Length=573, Percent_Identity=28.7958115183246, Blast_Score=172, Evalue=7e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.2.38 [H]

Molecular weight: Translated: 59136; Mature: 59004

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSGGDLLARALADAGVTEVFTLHGGHLDAFLVACGKHDVRLTDTRHESSAGHAADAYA
CCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHH
RVTGSLGVCVVTSGPGFTNAYTAIANGYLDRTPTLFIVGGPPLRESATNPLQGGFDQVAA
HCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHH
AAPVTKWAYRLTEAARIPEIVSLAVRKATTGAPGPVLLEVPIDVMFGEADEAEVRYPARY
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCCCCCCCCHHE
ALDARSVPSDSVVHQALDVLQGAQHPVIVIGGGVTFSRAADALVAFAETVGVPVFYPGKS
EECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCC
DGAIPASHRLAAGGLLGLGALSALGAPTPDVVLLVGARAGMFTGGRASMFPGARIVQVDL
CCCCCCCCCHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEC
DAAEIGRMQDVAVAIVADCRETLRALQAAAADRDWPDWSEWVHTAVQAQRFHRALYPDAA
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TPSGRMHPYFAARAVVEACPPGTTFVLDGAEAPAWAEFFATTETVGGVLRLGYLGTLGVG
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCC
PGFAIGAARARPAAPVVLITGDGAAGFHLGEFDTMARHGMPVLTVVFNNAVWGMSVHGQQ
CCCEECCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEEECCCE
AVYGDGGVVVSELADSSYEKVAEAFGGYGERVGEVDEIAAAVQRAFAAQVPACLNLEIDP
EEECCCCEEHHHHHCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECC
HTVHPLTTMMLGDVTATDAIVVPYYENLPR
CCCCHHHHHHHCCCCCCCEEEECCHHCCCC
>Mature Secondary Structure 
TVSGGDLLARALADAGVTEVFTLHGGHLDAFLVACGKHDVRLTDTRHESSAGHAADAYA
CCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHH
RVTGSLGVCVVTSGPGFTNAYTAIANGYLDRTPTLFIVGGPPLRESATNPLQGGFDQVAA
HCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHH
AAPVTKWAYRLTEAARIPEIVSLAVRKATTGAPGPVLLEVPIDVMFGEADEAEVRYPARY
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCCCCCCCCHHE
ALDARSVPSDSVVHQALDVLQGAQHPVIVIGGGVTFSRAADALVAFAETVGVPVFYPGKS
EECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCC
DGAIPASHRLAAGGLLGLGALSALGAPTPDVVLLVGARAGMFTGGRASMFPGARIVQVDL
CCCCCCCCCHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEC
DAAEIGRMQDVAVAIVADCRETLRALQAAAADRDWPDWSEWVHTAVQAQRFHRALYPDAA
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TPSGRMHPYFAARAVVEACPPGTTFVLDGAEAPAWAEFFATTETVGGVLRLGYLGTLGVG
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCC
PGFAIGAARARPAAPVVLITGDGAAGFHLGEFDTMARHGMPVLTVVFNNAVWGMSVHGQQ
CCCEECCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEEECCCE
AVYGDGGVVVSELADSSYEKVAEAFGGYGERVGEVDEIAAAVQRAFAAQVPACLNLEIDP
EEECCCCEEHHHHHCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECC
HTVHPLTTMMLGDVTATDAIVVPYYENLPR
CCCCHHHHHHHCCCCCCCEEEECCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8026749 [H]