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The map label for this gene is lexA

Identifier: 119868250

GI number: 119868250

Start: 2331197

End: 2331889

Strand: Reverse

Name: lexA

Synonym: Mkms_2214

Alternate gene names: 119868250

Gene position: 2331889-2331197 (Counterclockwise)

Preceding gene: 119868253

Following gene: 119868248

Centisome position: 40.64

GC content: 70.27

Gene sequence:

>693_bases
ATGAGCGACGACAGCAGCGAGACCCGGACCGGCGGGCGCCGCGGCGCAGACGCCGGCCTGACCGAACGACAACGCACCAT
CCTCGAGGTCATCCGCGCCTCGGTGACCAGCCGCGGCTACCCCCCGAGCATCCGGGAGATCGGCGACGCCGTCGGCCTGA
CGTCGACGTCCTCGGTGGCGCATCAGCTGCGCACACTCGAACGCAAGGGCTATCTGCGGCGTGACCCCAACCGGCCCCGC
GCCGTCGACGTACGCCTGTCCGACGAGCCGGCGACGCCGGTGGTCACCACGGACGTCGCGGGCAGCGACGCGCTACCCGA
ACCGACGTTCGTACCGGTGCTCGGCCGGATCGCCGCCGGAGGGCCGATCCTGGCGGAGGAAGCGGTCGAGGACGTGTTCC
CGCTACCGCGCGAACTCGTCGGCGAGGGGTCGCTGTTCCTGCTCAAGGTGGTGGGCGACTCGATGGTCGACGCCGCGATC
TGCGACGGCGACTGGGTCGTCGTCCGGCAACAGGCCGTCGCCGACAACGGCGACATCGTCGCGGCCATGATCGACGGCGA
GGCCACCGTGAAAACCTTCAAACGCAGCCGCGGTCAGGTGTGGCTGATGCCGCACAACCCCGCGTTCGAGCCCATCCCCG
GTAACGACGCGGCGGTGCTGGGCAAGGTCGTCACGGTCATCCGCAAGATCTGA

Upstream 100 bases:

>100_bases
GTTGCCGACGATAAGCGAGGGGTCCGACAAGTCCGTCCGACCACGACACGCGTCGAACACATGTTTGATCATCGGGCGTC
GGGCGACTAGATTCAGGCCC

Downstream 100 bases:

>100_bases
CGCCGGCTCAGTCGCCGCGGACGAACCCGTTGGTGCGGGCGAACTCCTCACTGGCGAAGTAGATCTCCGCGACCGCGTCG
TCGTAGCCACGGCTGCCCGG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MSDDSSETRTGGRRGADAGLTERQRTILEVIRASVTSRGYPPSIREIGDAVGLTSTSSVAHQLRTLERKGYLRRDPNRPR
AVDVRLSDEPATPVVTTDVAGSDALPEPTFVPVLGRIAAGGPILAEEAVEDVFPLPRELVGEGSLFLLKVVGDSMVDAAI
CDGDWVVVRQQAVADNGDIVAAMIDGEATVKTFKRSRGQVWLMPHNPAFEPIPGNDAAVLGKVVTVIRKI

Sequences:

>Translated_230_residues
MSDDSSETRTGGRRGADAGLTERQRTILEVIRASVTSRGYPPSIREIGDAVGLTSTSSVAHQLRTLERKGYLRRDPNRPR
AVDVRLSDEPATPVVTTDVAGSDALPEPTFVPVLGRIAAGGPILAEEAVEDVFPLPRELVGEGSLFLLKVVGDSMVDAAI
CDGDWVVVRQQAVADNGDIVAAMIDGEATVKTFKRSRGQVWLMPHNPAFEPIPGNDAAVLGKVVTVIRKI
>Mature_229_residues
SDDSSETRTGGRRGADAGLTERQRTILEVIRASVTSRGYPPSIREIGDAVGLTSTSSVAHQLRTLERKGYLRRDPNRPRA
VDVRLSDEPATPVVTTDVAGSDALPEPTFVPVLGRIAAGGPILAEEAVEDVFPLPRELVGEGSLFLLKVVGDSMVDAAIC
DGDWVVVRQQAVADNGDIVAAMIDGEATVKTFKRSRGQVWLMPHNPAFEPIPGNDAAVLGKVVTVIRKI

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=193, Percent_Identity=32.6424870466321, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787431, Length=118, Percent_Identity=36.4406779661017, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_MYCSJ (A3PYG6)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001070434.1
- ProteinModelPortal:   A3PYG6
- SMR:   A3PYG6
- STRING:   A3PYG6
- MEROPS:   S24.001
- EnsemblBacteria:   EBMYCT00000048543
- GeneID:   4877877
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_2157
- eggNOG:   COG1974
- GeneTree:   EBGT00050000016631
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- PhylomeDB:   A3PYG6
- ProtClustDB:   PRK00215
- BioCyc:   MSP164757:MJLS_2157-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 24560; Mature: 24429

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: ACT_SITE 154-154 ACT_SITE 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDSSETRTGGRRGADAGLTERQRTILEVIRASVTSRGYPPSIREIGDAVGLTSTSSVA
CCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHH
HQLRTLERKGYLRRDPNRPRAVDVRLSDEPATPVVTTDVAGSDALPEPTFVPVLGRIAAG
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCC
GPILAEEAVEDVFPLPRELVGEGSLFLLKVVGDSMVDAAICDGDWVVVRQQAVADNGDIV
CCHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCHHHHHEEECCCEEEEEHHHHCCCCCEE
AAMIDGEATVKTFKRSRGQVWLMPHNPAFEPIPGNDAAVLGKVVTVIRKI
EEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDDSSETRTGGRRGADAGLTERQRTILEVIRASVTSRGYPPSIREIGDAVGLTSTSSVA
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHH
HQLRTLERKGYLRRDPNRPRAVDVRLSDEPATPVVTTDVAGSDALPEPTFVPVLGRIAAG
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCC
GPILAEEAVEDVFPLPRELVGEGSLFLLKVVGDSMVDAAICDGDWVVVRQQAVADNGDIV
CCHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCHHHHHEEECCCEEEEEHHHHCCCCCEE
AAMIDGEATVKTFKRSRGQVWLMPHNPAFEPIPGNDAAVLGKVVTVIRKI
EEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA