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The map label for this gene is hyb0 [C]

Identifier: 119868226

GI number: 119868226

Start: 2307111

End: 2308166

Strand: Reverse

Name: hyb0 [C]

Synonym: Mkms_2190

Alternate gene names: 119868226

Gene position: 2308166-2307111 (Counterclockwise)

Preceding gene: 119868227

Following gene: 119868225

Centisome position: 40.23

GC content: 64.02

Gene sequence:

>1056_bases
ATGCCGACTGAGGCAGCAGTCAAAGCGGAAGAGGCGCTGATCCATGTTCTCTGGATCAATGCCGGATTGAGTTGCGACGG
TGATTCTGTGGCGCTGACTGCCGCCACCCAGCCCAGTATCGAGGAGATCGCGCTCGGCGCCCTCCCCGGACTGCCCAAGA
TCGCCGTTCACTGGCCCCTGATCGATTTCGAATGCGGACCGAACGGGGGCGCCGACGACTTCCTCGAATGGTTCTTCAGA
GCGGACAGAGGCGAATTGGAACCGTTCGTGTTGGTCGTCGAGGGATCCATCCCGAACGAGGCGATCAAGGACGAGGGATA
TTGGTGCGGGTTCGGTAACAACCCGGCCACCGGCCAACCGATGACCACCAGCGAGTGGCTCGACCGGTTGACGCCCAAGG
CGACCGCCGTGGTCGCGGTGGGCACCTGCGCGACCTACGGCGGCATCCACGCCATGGCGGGTAATCCCACCGGAGCCATG
GGTGTGCCCGACTATCTCGGCTGGGACTGGAAGAGCAAGGCGGGCATACCGATCGTCTGCGTTCCGGGGTGCCCGATACA
TCCGGACAACCTGGCCGAGACGCTCACCTACCTGCTCTACATGGCCACCGATCAGGCGCCGATGATCCCGTTGGACGATG
CTCTGCGGCCGCAGTGGCTTTTCGGTCAGTCAGTACACGAGGGGTGTGACCGCGCCGGCTACTACGAGCAGGGTGATTTC
GCCACCGAGTACGGGTCGCCGAAATGCATTGTGAAGCTGGGCTGTTGGGGCCCGGTGGTGAAGTGCAACGTGCCCAAGCG
AGGATGGATCAACGGTGTCGGGGGGTGCCCCAACGTCGGCGGCATCTGTATCGGATGCACCATGCCCGGCTTCCCGGACA
AGTTCATGCCGTTCATGGACGAACCACCGGGCGGCAAGGTCTCGACCACCGCATCCACCCTGTACGGATCGGTCATCCGC
AGCCTGCGCCACGTCACCGGCCGCACCGTCGACAAAGAACCGAAGTGGCGCCACCCCGGCACGAAACTCGAGACGGGCGC
GACGCGCACCTGGTAG

Upstream 100 bases:

>100_bases
TCGCGTCGGAGTAGACCCAGAACTAGCGCCGCGCAAGTGGGCCGAGGGTCGACTCCGGCGGCGCAGGGGTCGTCAGCCCC
GACCCCGGAAAGCTGTGGCG

Downstream 100 bases:

>100_bases
GTGCGCGTCGTCGCGTCCGCACCTGCGCGCCGGAGTCGGCTGATCCCCGTCGGCGCGCGGATTGCTGAGCACGAGAGAAG
GATTCGATGACCACGACTAT

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MPTEAAVKAEEALIHVLWINAGLSCDGDSVALTAATQPSIEEIALGALPGLPKIAVHWPLIDFECGPNGGADDFLEWFFR
ADRGELEPFVLVVEGSIPNEAIKDEGYWCGFGNNPATGQPMTTSEWLDRLTPKATAVVAVGTCATYGGIHAMAGNPTGAM
GVPDYLGWDWKSKAGIPIVCVPGCPIHPDNLAETLTYLLYMATDQAPMIPLDDALRPQWLFGQSVHEGCDRAGYYEQGDF
ATEYGSPKCIVKLGCWGPVVKCNVPKRGWINGVGGCPNVGGICIGCTMPGFPDKFMPFMDEPPGGKVSTTASTLYGSVIR
SLRHVTGRTVDKEPKWRHPGTKLETGATRTW

Sequences:

>Translated_351_residues
MPTEAAVKAEEALIHVLWINAGLSCDGDSVALTAATQPSIEEIALGALPGLPKIAVHWPLIDFECGPNGGADDFLEWFFR
ADRGELEPFVLVVEGSIPNEAIKDEGYWCGFGNNPATGQPMTTSEWLDRLTPKATAVVAVGTCATYGGIHAMAGNPTGAM
GVPDYLGWDWKSKAGIPIVCVPGCPIHPDNLAETLTYLLYMATDQAPMIPLDDALRPQWLFGQSVHEGCDRAGYYEQGDF
ATEYGSPKCIVKLGCWGPVVKCNVPKRGWINGVGGCPNVGGICIGCTMPGFPDKFMPFMDEPPGGKVSTTASTLYGSVIR
SLRHVTGRTVDKEPKWRHPGTKLETGATRTW
>Mature_350_residues
PTEAAVKAEEALIHVLWINAGLSCDGDSVALTAATQPSIEEIALGALPGLPKIAVHWPLIDFECGPNGGADDFLEWFFRA
DRGELEPFVLVVEGSIPNEAIKDEGYWCGFGNNPATGQPMTTSEWLDRLTPKATAVVAVGTCATYGGIHAMAGNPTGAMG
VPDYLGWDWKSKAGIPIVCVPGCPIHPDNLAETLTYLLYMATDQAPMIPLDDALRPQWLFGQSVHEGCDRAGYYEQGDFA
TEYGSPKCIVKLGCWGPVVKCNVPKRGWINGVGGCPNVGGICIGCTMPGFPDKFMPFMDEPPGGKVSTTASTLYGSVIRS
LRHVTGRTVDKEPKWRHPGTKLETGATRTW

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=304, Percent_Identity=28.6184210526316, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787206, Length=305, Percent_Identity=27.2131147540984, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 37626; Mature: 37495

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEAAVKAEEALIHVLWINAGLSCDGDSVALTAATQPSIEEIALGALPGLPKIAVHWPL
CCCHHHHHHHHEEEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEEEEE
IDFECGPNGGADDFLEWFFRADRGELEPFVLVVEGSIPNEAIKDEGYWCGFGNNPATGQP
EEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHCCCCEEEECCCCCCCCCC
MTTSEWLDRLTPKATAVVAVGTCATYGGIHAMAGNPTGAMGVPDYLGWDWKSKAGIPIVC
CCHHHHHHHCCCCCEEEEEECCHHHHCCEEEECCCCCCCCCCCHHHCCCCCCCCCCCEEE
VPGCPIHPDNLAETLTYLLYMATDQAPMIPLDDALRPQWLFGQSVHEGCDRAGYYEQGDF
CCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCCE
ATEYGSPKCIVKLGCWGPVVKCNVPKRGWINGVGGCPNVGGICIGCTMPGFPDKFMPFMD
ECCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHCCCCC
EPPGGKVSTTASTLYGSVIRSLRHVTGRTVDKEPKWRHPGTKLETGATRTW
CCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTEAAVKAEEALIHVLWINAGLSCDGDSVALTAATQPSIEEIALGALPGLPKIAVHWPL
CCHHHHHHHHEEEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEEEEE
IDFECGPNGGADDFLEWFFRADRGELEPFVLVVEGSIPNEAIKDEGYWCGFGNNPATGQP
EEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHCCCCEEEECCCCCCCCCC
MTTSEWLDRLTPKATAVVAVGTCATYGGIHAMAGNPTGAMGVPDYLGWDWKSKAGIPIVC
CCHHHHHHHCCCCCEEEEEECCHHHHCCEEEECCCCCCCCCCCHHHCCCCCCCCCCCEEE
VPGCPIHPDNLAETLTYLLYMATDQAPMIPLDDALRPQWLFGQSVHEGCDRAGYYEQGDF
CCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCCE
ATEYGSPKCIVKLGCWGPVVKCNVPKRGWINGVGGCPNVGGICIGCTMPGFPDKFMPFMD
ECCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHCCCCC
EPPGGKVSTTASTLYGSVIRSLRHVTGRTVDKEPKWRHPGTKLETGATRTW
CCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]