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The map label for this gene is hyaB [C]

Identifier: 119868225

GI number: 119868225

Start: 2305228

End: 2307024

Strand: Reverse

Name: hyaB [C]

Synonym: Mkms_2189

Alternate gene names: 119868225

Gene position: 2307024-2305228 (Counterclockwise)

Preceding gene: 119868226

Following gene: 119868224

Centisome position: 40.21

GC content: 63.94

Gene sequence:

>1797_bases
ATGACCACGACTATCCCCGACCCGTCGCACGTCAAGCGCGAACCCGGCCAACTGGTGGAGATGGCCTGGGACCCCATCAC
CCGCATCGTCGGCAGCCTCGGCATCTACACCAAGGTCGACTTCGAGAACCGCGAAGTCGTGGAGTGCCACAGCACCTCGT
CGATCTTCCGCGGCTACTCCATCTTCATGAAGGGCAAGGATCCGCGCGACGCCCACTTCATCACCAGCCGGATCTGCGGC
ATCTGCGGGGACAACCATGCCACGTGCTCGTGTTACACGCAGAACATGGCCTACGGGGTGCAGCCGCCGCACCTGGCCGA
GTGGTTGGTCAACCTCGGCGAGGCCGCGGAATACATGTTCGACCACAACATCTTCCAGGAGAACCTCGTGGGGGTCGACT
TCTGCGAGAAGATGGTCGCCGAGACCAACCCGTCGGTGCTGGCCAAGGCGGAGAACGCCCAGGCGCCGCACGCCGACATG
CACGGCTACCGCACGATCGCCGACATCATGCGGGCGCTCAACCCGTTCACCGGCGAGTTCTACCGGGAGGCGCTGCAGGT
CAGCCGTTACACACGAGAGATGTTCTGTCTCATGGAAGGTCGGCACGTCCACCCGTCGACCCTGTATCCCGGCGGGATCG
GCACCACGGCGACCGTCCAGCTGATGACCGACTACATGACCCGGCTGATGCGCTACGTGGAGTTCATGAAGAAGGTCGTG
CCGATGCACGACGATCTGTTCGACTTCTTCTACGAAGCGCTGCCCGGCTACGACCAGGTGGGGCTGCGACGCACGCTGCT
CGGCTGCTGGGGCTCGTTCCAGGATCCCGAGCACTGCAACTTCAGCTACAAGGACATGGAGGCCTGGGGTCGCAAGATGT
TCGTCACCCCCGGTGTCGTGGTGGACGGCAAACTCGTCACCACATCGCTGGTCGACATCAACCTGGGCATCCGAATCCTG
TTGGGTCACAGTTACTACGAGGACTGGACCGATCAGGAGATGTTCGTCAAGACCGACCCGCTGGGCAACCCGGTCGACCG
GCGGCACCCGTGGAATCAGCACACCAACCCGAAGCCGCAGAAGCGCGACTTCGACGAGAACTACAGCTGGGTGATGTCAC
CGCGCTGGTTCGACGGCAACGACCACCTGGCACTGGACACCGGCGGCGGGCCGCTGGCGCGTCTGTGGTCGACGGCACTG
GCGAATCTCGTCGACATCGGCTACGTGAAGTCGACCGGTCACAGTGTGCAGATCAACCTGCCCAAGACTGCGCTCAAGGG
TCCGGTGGAACTCGAATGGAAGATCCCGCAGTACGGCAGCAACACGCTCGAACGCAACCGGGCCCGCACGTACTTCCAGG
CCTATGCGGCGGCGTGCGCGCTGCACTTCGCCGAGAAGGCGCTGACGGAGATCCGGGCCGGCCGCACCAAGACGTGGGAG
CGTTTCGAGGTGCCCGACGAGGGTATCGGCTGCGGCTTCACCGAGGCGGTGCGCGGCGTGCTCAGCCACCACATGGTGAT
TCGCGACGGCAAGATCGCGAACTACCACCCGTATCCGCCGACGCCGTGGAACGCCAACCCGCGCGACAGCTACGGCACAC
CGGGACCGTACGAGGATGCCGTACAGGGTCAACCGATCTTCGAGGAGAACGGTCGGGAGAAGTTCAAGGGCATCGACATC
ATGCGGACGGTGCGCAGCTTCGACCCGTGTCTGCCCTGCGGGGTGCACATGTACCTGGGCGGCGGGAAGTCGTTGGAGTT
GCTGCACTCCCCCACCCAGTCCGTCACCGGGAAATAG

Upstream 100 bases:

>100_bases
CGCGCACCTGGTAGGTGCGCGTCGTCGCGTCCGCACCTGCGCGCCGGAGTCGGCTGATCCCCGTCGGCGCGCGGATTGCT
GAGCACGAGAGAAGGATTCG

Downstream 100 bases:

>100_bases
GGCATGTGTCCGGCCGCGCCGAGTGAACACGATGCGGACGCGGACACGCGCTGGCGCACGGCGGGCGAGCGCATCCAGAC
GCTGCTGGACGCCAGTTCGG

Product: hydrogen:quinone oxidoreductase

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MTTTIPDPSHVKREPGQLVEMAWDPITRIVGSLGIYTKVDFENREVVECHSTSSIFRGYSIFMKGKDPRDAHFITSRICG
ICGDNHATCSCYTQNMAYGVQPPHLAEWLVNLGEAAEYMFDHNIFQENLVGVDFCEKMVAETNPSVLAKAENAQAPHADM
HGYRTIADIMRALNPFTGEFYREALQVSRYTREMFCLMEGRHVHPSTLYPGGIGTTATVQLMTDYMTRLMRYVEFMKKVV
PMHDDLFDFFYEALPGYDQVGLRRTLLGCWGSFQDPEHCNFSYKDMEAWGRKMFVTPGVVVDGKLVTTSLVDINLGIRIL
LGHSYYEDWTDQEMFVKTDPLGNPVDRRHPWNQHTNPKPQKRDFDENYSWVMSPRWFDGNDHLALDTGGGPLARLWSTAL
ANLVDIGYVKSTGHSVQINLPKTALKGPVELEWKIPQYGSNTLERNRARTYFQAYAAACALHFAEKALTEIRAGRTKTWE
RFEVPDEGIGCGFTEAVRGVLSHHMVIRDGKIANYHPYPPTPWNANPRDSYGTPGPYEDAVQGQPIFEENGREKFKGIDI
MRTVRSFDPCLPCGVHMYLGGGKSLELLHSPTQSVTGK

Sequences:

>Translated_598_residues
MTTTIPDPSHVKREPGQLVEMAWDPITRIVGSLGIYTKVDFENREVVECHSTSSIFRGYSIFMKGKDPRDAHFITSRICG
ICGDNHATCSCYTQNMAYGVQPPHLAEWLVNLGEAAEYMFDHNIFQENLVGVDFCEKMVAETNPSVLAKAENAQAPHADM
HGYRTIADIMRALNPFTGEFYREALQVSRYTREMFCLMEGRHVHPSTLYPGGIGTTATVQLMTDYMTRLMRYVEFMKKVV
PMHDDLFDFFYEALPGYDQVGLRRTLLGCWGSFQDPEHCNFSYKDMEAWGRKMFVTPGVVVDGKLVTTSLVDINLGIRIL
LGHSYYEDWTDQEMFVKTDPLGNPVDRRHPWNQHTNPKPQKRDFDENYSWVMSPRWFDGNDHLALDTGGGPLARLWSTAL
ANLVDIGYVKSTGHSVQINLPKTALKGPVELEWKIPQYGSNTLERNRARTYFQAYAAACALHFAEKALTEIRAGRTKTWE
RFEVPDEGIGCGFTEAVRGVLSHHMVIRDGKIANYHPYPPTPWNANPRDSYGTPGPYEDAVQGQPIFEENGREKFKGIDI
MRTVRSFDPCLPCGVHMYLGGGKSLELLHSPTQSVTGK
>Mature_597_residues
TTTIPDPSHVKREPGQLVEMAWDPITRIVGSLGIYTKVDFENREVVECHSTSSIFRGYSIFMKGKDPRDAHFITSRICGI
CGDNHATCSCYTQNMAYGVQPPHLAEWLVNLGEAAEYMFDHNIFQENLVGVDFCEKMVAETNPSVLAKAENAQAPHADMH
GYRTIADIMRALNPFTGEFYREALQVSRYTREMFCLMEGRHVHPSTLYPGGIGTTATVQLMTDYMTRLMRYVEFMKKVVP
MHDDLFDFFYEALPGYDQVGLRRTLLGCWGSFQDPEHCNFSYKDMEAWGRKMFVTPGVVVDGKLVTTSLVDINLGIRILL
GHSYYEDWTDQEMFVKTDPLGNPVDRRHPWNQHTNPKPQKRDFDENYSWVMSPRWFDGNDHLALDTGGGPLARLWSTALA
NLVDIGYVKSTGHSVQINLPKTALKGPVELEWKIPQYGSNTLERNRARTYFQAYAAACALHFAEKALTEIRAGRTKTWER
FEVPDEGIGCGFTEAVRGVLSHHMVIRDGKIANYHPYPPTPWNANPRDSYGTPGPYEDAVQGQPIFEENGREKFKGIDIM
RTVRSFDPCLPCGVHMYLGGGKSLELLHSPTQSVTGK

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=616, Percent_Identity=27.4350649350649, Blast_Score=170, Evalue=3e-43,
Organism=Escherichia coli, GI1789368, Length=609, Percent_Identity=25.7799671592775, Blast_Score=156, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 67702; Mature: 67570

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTIPDPSHVKREPGQLVEMAWDPITRIVGSLGIYTKVDFENREVVECHSTSSIFRGYS
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCHHHHHCCEE
IFMKGKDPRDAHFITSRICGICGDNHATCSCYTQNMAYGVQPPHLAEWLVNLGEAAEYMF
EEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
DHNIFQENLVGVDFCEKMVAETNPSVLAKAENAQAPHADMHGYRTIADIMRALNPFTGEF
HHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
YREALQVSRYTREMFCLMEGRHVHPSTLYPGGIGTTATVQLMTDYMTRLMRYVEFMKKVV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PMHDDLFDFFYEALPGYDQVGLRRTLLGCWGSFQDPEHCNFSYKDMEAWGRKMFVTPGVV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCEEEECCCEE
VDGKLVTTSLVDINLGIRILLGHSYYEDWTDQEMFVKTDPLGNPVDRRHPWNQHTNPKPQ
ECCEEEEEEEEEECCCEEEEECCCHHCCCCCCEEEEEECCCCCCHHCCCCCCCCCCCCCC
KRDFDENYSWVMSPRWFDGNDHLALDTGGGPLARLWSTALANLVDIGYVKSTGHSVQINL
CCCCCCCCCEEECCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCEEECCCCEEEEEC
PKTALKGPVELEWKIPQYGSNTLERNRARTYFQAYAAACALHFAEKALTEIRAGRTKTWE
CHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RFEVPDEGIGCGFTEAVRGVLSHHMVIRDGKIANYHPYPPTPWNANPRDSYGTPGPYEDA
CCCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VQGQPIFEENGREKFKGIDIMRTVRSFDPCLPCGVHMYLGGGKSLELLHSPTQSVTGK
CCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCHHCCCC
>Mature Secondary Structure 
TTTIPDPSHVKREPGQLVEMAWDPITRIVGSLGIYTKVDFENREVVECHSTSSIFRGYS
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCHHHHHCCEE
IFMKGKDPRDAHFITSRICGICGDNHATCSCYTQNMAYGVQPPHLAEWLVNLGEAAEYMF
EEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
DHNIFQENLVGVDFCEKMVAETNPSVLAKAENAQAPHADMHGYRTIADIMRALNPFTGEF
HHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
YREALQVSRYTREMFCLMEGRHVHPSTLYPGGIGTTATVQLMTDYMTRLMRYVEFMKKVV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PMHDDLFDFFYEALPGYDQVGLRRTLLGCWGSFQDPEHCNFSYKDMEAWGRKMFVTPGVV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCEEEECCCEE
VDGKLVTTSLVDINLGIRILLGHSYYEDWTDQEMFVKTDPLGNPVDRRHPWNQHTNPKPQ
ECCEEEEEEEEEECCCEEEEECCCHHCCCCCCEEEEEECCCCCCHHCCCCCCCCCCCCCC
KRDFDENYSWVMSPRWFDGNDHLALDTGGGPLARLWSTALANLVDIGYVKSTGHSVQINL
CCCCCCCCCEEECCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCEEECCCCEEEEEC
PKTALKGPVELEWKIPQYGSNTLERNRARTYFQAYAAACALHFAEKALTEIRAGRTKTWE
CHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RFEVPDEGIGCGFTEAVRGVLSHHMVIRDGKIANYHPYPPTPWNANPRDSYGTPGPYEDA
CCCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VQGQPIFEENGREKFKGIDIMRTVRSFDPCLPCGVHMYLGGGKSLELLHSPTQSVTGK
CCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]