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The map label for this gene is dacB [H]

Identifier: 119868045

GI number: 119868045

Start: 2131329

End: 2132204

Strand: Reverse

Name: dacB [H]

Synonym: Mkms_2008

Alternate gene names: 119868045

Gene position: 2132204-2131329 (Counterclockwise)

Preceding gene: 119868047

Following gene: 119868044

Centisome position: 37.16

GC content: 70.89

Gene sequence:

>876_bases
ATGGGGAGGCTCGTCAGTCTGTTCGCAATCGTCCTGGCCGCACTCGGCGTCGTCGCCGCGCCCGTCGCCGCCGCCGACAT
CGACATCCAGCCGGTGGGGTCGGTACCGATCCCCGCCGGTCCGGCGGAGGCGTGGATCGTGGCGGACATGGACACCGGTC
AGGTGCTGGCCGGCCGCAACGACACCACGCGTTACGCCCCCGCGAGCACGATCAAGACACTGCTGGCGCAGGTGGTGCTC
GACGAGGTGCCGCTGGACACGACGATCGCCGCCGAGGAGGCCGACACCAGGGTGGAGTGCAACTGCGCCGGGGTGGCGCC
CGGCCACGTCTACACGGCCCGCCAACTGCTCGAGGCGCTGCTGCTGGTGTCGGGCAATGACGCCGCCAACACCCTGGCTC
GCATGCTCGGCGGCATGGACTCCGCCGTGGCGAAGATGAACGCCAAGGCCGCCCAACTGGGCGCCCACGGCACCAACGTG
GTGACTCCGTCCGGCCTGGACGGACCCGGGATGCCGTTCTGGTCCACCGCCCACGATCTGGCGGTGATCTTCCGGGCGGC
GATGGCCAACCCGGTGTTCGCGCAGATCACCGCGATGCCGTCGACGGTCTTCCCGACCAAGGCCGGGGAGGCCGTCCTGG
TCAACCAGAACGAGCTGCTGCACCGCTACCCCGGCGCCATCGGCGGCAAGACGGGGTTCACCGACATCGCCCGCAAGACC
TTCGTCGGCGCGGCGCAGCGCGACGGCCGACGGTTGGTGGTCGTGCTGATGCACGGGCTGGTCAAAGAGGGCGGCCCCAC
CTACTGGGATCAGGCGGCCGGCCTGCTCGACTGGGGCTTCGGGCTCGACCGCGGCGCGAGCGTCGGAACCCTGTAG

Upstream 100 bases:

>100_bases
GCCGAGGAGTAGTCGACCATCAGCTGCTGGATCTCCAGCCGGTCCGAGATTTCCGCCAGGCTCAACATGGCTCGATTGAA
CACGATTAGGCTCACCCGCC

Downstream 100 bases:

>100_bases
CGCCTGACACAAACAGAGTGTCATTGAGCTGCAACGGTTTCGAGACCGATCCGAGGCGTAAGCGCCGTTAGGCTCTGGCG
CCGTGGCCTGTTCGCGTTCG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MGRLVSLFAIVLAALGVVAAPVAAADIDIQPVGSVPIPAGPAEAWIVADMDTGQVLAGRNDTTRYAPASTIKTLLAQVVL
DEVPLDTTIAAEEADTRVECNCAGVAPGHVYTARQLLEALLLVSGNDAANTLARMLGGMDSAVAKMNAKAAQLGAHGTNV
VTPSGLDGPGMPFWSTAHDLAVIFRAAMANPVFAQITAMPSTVFPTKAGEAVLVNQNELLHRYPGAIGGKTGFTDIARKT
FVGAAQRDGRRLVVVLMHGLVKEGGPTYWDQAAGLLDWGFGLDRGASVGTL

Sequences:

>Translated_291_residues
MGRLVSLFAIVLAALGVVAAPVAAADIDIQPVGSVPIPAGPAEAWIVADMDTGQVLAGRNDTTRYAPASTIKTLLAQVVL
DEVPLDTTIAAEEADTRVECNCAGVAPGHVYTARQLLEALLLVSGNDAANTLARMLGGMDSAVAKMNAKAAQLGAHGTNV
VTPSGLDGPGMPFWSTAHDLAVIFRAAMANPVFAQITAMPSTVFPTKAGEAVLVNQNELLHRYPGAIGGKTGFTDIARKT
FVGAAQRDGRRLVVVLMHGLVKEGGPTYWDQAAGLLDWGFGLDRGASVGTL
>Mature_290_residues
GRLVSLFAIVLAALGVVAAPVAAADIDIQPVGSVPIPAGPAEAWIVADMDTGQVLAGRNDTTRYAPASTIKTLLAQVVLD
EVPLDTTIAAEEADTRVECNCAGVAPGHVYTARQLLEALLLVSGNDAANTLARMLGGMDSAVAKMNAKAAQLGAHGTNVV
TPSGLDGPGMPFWSTAHDLAVIFRAAMANPVFAQITAMPSTVFPTKAGEAVLVNQNELLHRYPGAIGGKTGFTDIARKTF
VGAAQRDGRRLVVVLMHGLVKEGGPTYWDQAAGLLDWGFGLDRGASVGTL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=282, Percent_Identity=30.1418439716312, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786851, Length=297, Percent_Identity=27.9461279461279, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787062, Length=266, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082054, Length=288, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 30034; Mature: 29903

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLVSLFAIVLAALGVVAAPVAAADIDIQPVGSVPIPAGPAEAWIVADMDTGQVLAGRN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCC
DTTRYAPASTIKTLLAQVVLDEVPLDTTIAAEEADTRVECNCAGVAPGHVYTARQLLEAL
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHH
LLVSGNDAANTLARMLGGMDSAVAKMNAKAAQLGAHGTNVVTPSGLDGPGMPFWSTAHDL
HHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
AVIFRAAMANPVFAQITAMPSTVFPTKAGEAVLVNQNELLHRYPGAIGGKTGFTDIARKT
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCCCHHHHHHHH
FVGAAQRDGRRLVVVLMHGLVKEGGPTYWDQAAGLLDWGFGLDRGASVGTL
HHHHHHCCCCEEHHHHHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GRLVSLFAIVLAALGVVAAPVAAADIDIQPVGSVPIPAGPAEAWIVADMDTGQVLAGRN
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCC
DTTRYAPASTIKTLLAQVVLDEVPLDTTIAAEEADTRVECNCAGVAPGHVYTARQLLEAL
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHH
LLVSGNDAANTLARMLGGMDSAVAKMNAKAAQLGAHGTNVVTPSGLDGPGMPFWSTAHDL
HHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
AVIFRAAMANPVFAQITAMPSTVFPTKAGEAVLVNQNELLHRYPGAIGGKTGFTDIARKT
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHCCCCCCCCCCHHHHHHHH
FVGAAQRDGRRLVVVLMHGLVKEGGPTYWDQAAGLLDWGFGLDRGASVGTL
HHHHHHCCCCEEHHHHHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]