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The map label for this gene is dacF [H]

Identifier: 119868044

GI number: 119868044

Start: 2130392

End: 2131186

Strand: Reverse

Name: dacF [H]

Synonym: Mkms_2007

Alternate gene names: 119868044

Gene position: 2131186-2130392 (Counterclockwise)

Preceding gene: 119868045

Following gene: 119868037

Centisome position: 37.15

GC content: 69.56

Gene sequence:

>795_bases
ATGACCATGTCGTCGGTCGGCGTGGTGACGCGAACGCCTGCGGCGCTCGCCCAACCGCAACCGGCGGGCGCCGTCGCGCT
GCCCGAGGGACCGGCGCAGGCGTGGCTGCTCGCCGACCTCGATTCGGGCCGAGTCCTGGCCAGCCGCAACCCCTATGAAC
CCCACGCCCCCGCGAGCACGATCAAGGTGCTGCTCGCCATGGTGGTGCTCGACCACCTCTCCCCGGACAACTTCGCCAGA
GCCAACAGATCCCACACCGAGGTCGAGTGTTCCTGCGTCGGGCTCACCCCGGGCCAGCCCTACACCACCCGCCAGCTGCT
CTCGGCGCTGCTCATGGTGTCGGGCAACGACGCCGCCAACATGCTCGCCGACATGCTGGGCGGCCAGCGCGCCGCGGTCG
CGGCGATGAACCGCAAGGCCGCCCTCGTCGGCGCCAAGTCGACCAGGGCATCGTCACCGTCGGGCCTCGACGGGCCCGGC
TGGGAGTCGATCACCACCCCGCACGATCTGGCCGTGATGTTGCGGGCCGCGCTGAAGTATCCGCTGATCGCGCAGATCAT
GCGGTCACCGACGGCGCCGTTCCCCGGCAAGACCCTCACGAACCAGAACGAACTCCTGAGCCGCTACCCGGGCGACATCG
CCGGTAAGACCGGCTTCACGAACCTGGCCCGCAAGACCTATGTCGGTGCGGCGCAACGCGGCAACCGCCGCCTCGCGGTC
GTGCAGATGTACGGCACCGGCGACCTCTACGGTCAGGCCATCGGGTTGTTCGACTACGGCTTCAGCCAGCCCTAG

Upstream 100 bases:

>100_bases
AGACCGATCCGAGGCGTAAGCGCCGTTAGGCTCTGGCGCCGTGGCCTGTTCGCGTTCGCGTCGGTGGTCGCGGACGTTCA
TCGCGGGATGCGCGCTCGCG

Downstream 100 bases:

>100_bases
AAGACCCGGCCCCGCAGGATGACCAGCGCCGGCTCGTTGACCACCGCGGAGCCGAGGCGCGGATCTTGGTCGTAACACAC
CAGATCGGCGGGGGCGCCGT

Product: peptidase S11, D-alanyl-D-alanine carboxypeptidase 1

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTMSSVGVVTRTPAALAQPQPAGAVALPEGPAQAWLLADLDSGRVLASRNPYEPHAPASTIKVLLAMVVLDHLSPDNFAR
ANRSHTEVECSCVGLTPGQPYTTRQLLSALLMVSGNDAANMLADMLGGQRAAVAAMNRKAALVGAKSTRASSPSGLDGPG
WESITTPHDLAVMLRAALKYPLIAQIMRSPTAPFPGKTLTNQNELLSRYPGDIAGKTGFTNLARKTYVGAAQRGNRRLAV
VQMYGTGDLYGQAIGLFDYGFSQP

Sequences:

>Translated_264_residues
MTMSSVGVVTRTPAALAQPQPAGAVALPEGPAQAWLLADLDSGRVLASRNPYEPHAPASTIKVLLAMVVLDHLSPDNFAR
ANRSHTEVECSCVGLTPGQPYTTRQLLSALLMVSGNDAANMLADMLGGQRAAVAAMNRKAALVGAKSTRASSPSGLDGPG
WESITTPHDLAVMLRAALKYPLIAQIMRSPTAPFPGKTLTNQNELLSRYPGDIAGKTGFTNLARKTYVGAAQRGNRRLAV
VQMYGTGDLYGQAIGLFDYGFSQP
>Mature_263_residues
TMSSVGVVTRTPAALAQPQPAGAVALPEGPAQAWLLADLDSGRVLASRNPYEPHAPASTIKVLLAMVVLDHLSPDNFARA
NRSHTEVECSCVGLTPGQPYTTRQLLSALLMVSGNDAANMLADMLGGQRAAVAAMNRKAALVGAKSTRASSPSGLDGPGW
ESITTPHDLAVMLRAALKYPLIAQIMRSPTAPFPGKTLTNQNELLSRYPGDIAGKTGFTNLARKTYVGAAQRGNRRLAVV
QMYGTGDLYGQAIGLFDYGFSQP

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=239, Percent_Identity=33.4728033472803, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI87082054, Length=241, Percent_Identity=32.7800829875519, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787062, Length=265, Percent_Identity=29.4339622641509, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1786851, Length=233, Percent_Identity=29.6137339055794, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 27773; Mature: 27642

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSSVGVVTRTPAALAQPQPAGAVALPEGPAQAWLLADLDSGRVLASRNPYEPHAPAST
CCCCCCCCEEECCHHHCCCCCCCEEECCCCCCCEEEEEECCCCCEEECCCCCCCCCCHHH
IKVLLAMVVLDHLSPDNFARANRSHTEVECSCVGLTPGQPYTTRQLLSALLMVSGNDAAN
HHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHH
MLADMLGGQRAAVAAMNRKAALVGAKSTRASSPSGLDGPGWESITTPHDLAVMLRAALKY
HHHHHHCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PLIAQIMRSPTAPFPGKTLTNQNELLSRYPGDIAGKTGFTNLARKTYVGAAQRGNRRLAV
HHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
VQMYGTGDLYGQAIGLFDYGFSQP
EEEECCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TMSSVGVVTRTPAALAQPQPAGAVALPEGPAQAWLLADLDSGRVLASRNPYEPHAPAST
CCCCCCCEEECCHHHCCCCCCCEEECCCCCCCEEEEEECCCCCEEECCCCCCCCCCHHH
IKVLLAMVVLDHLSPDNFARANRSHTEVECSCVGLTPGQPYTTRQLLSALLMVSGNDAAN
HHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHH
MLADMLGGQRAAVAAMNRKAALVGAKSTRASSPSGLDGPGWESITTPHDLAVMLRAALKY
HHHHHHCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PLIAQIMRSPTAPFPGKTLTNQNELLSRYPGDIAGKTGFTNLARKTYVGAAQRGNRRLAV
HHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
VQMYGTGDLYGQAIGLFDYGFSQP
EEEECCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]