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The map label for this gene is ligB

Identifier: 119867885

GI number: 119867885

Start: 1960743

End: 1962305

Strand: Reverse

Name: ligB

Synonym: Mkms_1846

Alternate gene names: 119867885

Gene position: 1962305-1960743 (Counterclockwise)

Preceding gene: 119867886

Following gene: 119867884

Centisome position: 34.2

GC content: 72.87

Gene sequence:

>1563_bases
GTGCTGCTCGTCGATGTCGCCACCGCGTCGGTCGACGTCGGCGCGATGTCGTCGCGGCTGGCCAAGACGGCCAGGATCGC
CGATCTGCTGAGCCGCGCGGGCACCGAACAGGATGCCCGGCTCGTCGCGGTGACGGTGGCGTGGCTGTCCGGTGAACTCC
CCCAGCGCCAGATCGGTGTCGGCTGGGCGGCCTTGCGTTCGCTCCCGGCCCCGGCCGCCGCGCCCACACTGACCGTGACG
GCGGTCGACGCGGTGTTCTCCGAGATCGGCGCGGTGGCCGGAAAAGGTTCGCAGGCCCGCCGGGCGGGTCTCATCGCCGA
GTTGTTCGCCGCCGCCACCGACGTCGAGCAGACCTTCCTGCGCCGCCTGCTGACCGGGGAACTGCGTCAGGGGGCGTTGA
TCGGGGTGATGGCCGACGCCGTCGCCAAGGCCGCCGATGTGCCCGCCGCCCGGGTGCGCCGGGCGGCGATGCTCGCCGGT
GACCTGCCCGCGGTGGCCGCCGCTGTGCTCGCCGGAGGGGACGCCGCGCTGGCCCGGTTCACCCTGCAGGTGGGCCGGCC
CGTCGGGCCGATGCTCGCCCAGACCGCAACGGGAGTCGCCGACGCACTGGACCGGTTGGGCGGCACGGCGGTGTTCGAGG
CGAAACTCGACGGCGCCCGCGTGCAGATCCACCGTCGGGGATCGGACGTGTCGGTGTACACCCGATCCCTCGACGACGTC
ACCGCGCGCCTGCCGGAGGTCGTCGAGGCTGCGCTCGCACTGCCGGTCACCGACCTGATCGCCGACGCCGAGGCGATCGC
GCTGCGCCCGGACGGCCGCCCGCACCGGTTCCAGGTGACCGCATCGCGGTTCGGCCGGGCGGCAGCGCGCGCCACGCAGC
CGCTGTCGGTGTTCATGTTCGACCTGCTGCACGTCGACGGCGCCGATCTGCTCGATCAGCCGACCAGCGACCGGGTGCGC
GTCCTCGACGACCTCGTGCCCGCGGCGCACCGCGTCGACCGGCTCGTCACCGACGACGGCGCCGCCGCGCAGCGGTTCCT
GGAGGCGACGCTCGCCGCCGGACACGAGGGCGTGATGGCGAAGTCACCGAACGCGCCGTACGAAGCGGGCCGCCGCGGGG
CGGGCTGGTTGAAGGTCAAACCCGTCCACACCCTCGATCTGGTCGTGCTCGCCGTCGAATGGGGCAGCGGCAGGCGCACC
GGGAAGCTGTCCAACATCCACCTCGGCGCCCGCGACCCCGCGACCGGGGGTTTCGTCATGCTGGGCAAGACGTTCAAGGG
GATGACCGACGCGATGCTCGACTGGCAGACCGCCCGGTTCCTCGAACTGGCCGACCCAGCCGCGCAGCCGGCGACATCCG
GCCGCGATCCGACCGACGGCCACACCGTCAAGGTCCGGCCCGAACAGGTGGTCGAGATCGCGTTCGACGGCGTCCAGGGT
TCGACGCGGTATCCGGGCGGCATGGCATTGCGGTTCGCCCGCGTCCTGCGCTACCGCGACGACAAATCGCCGGCCGAGGC
GGATACGGTCGACACCGTCCGTGCCTTCTACGAGCACGGCTGA

Upstream 100 bases:

>100_bases
CGCCCGCGGGTTCCCCGATCGCGCGGCGACGCTGCGCGGCGAAGCCGAGGTGCTCGAGGCTCTGCTGGGGAATGTCTGAC
CGGCGTGCCATCCTCGACGT

Downstream 100 bases:

>100_bases
AACCCCGGAATCGGCGCACGGGCGATCTGGCCGGTGCAAGTATCGACACCACGGGCATCTGACACTGTCGCGCCATCGTA
GGGAGGGATCGTGGCCACAC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MLLVDVATASVDVGAMSSRLAKTARIADLLSRAGTEQDARLVAVTVAWLSGELPQRQIGVGWAALRSLPAPAAAPTLTVT
AVDAVFSEIGAVAGKGSQARRAGLIAELFAAATDVEQTFLRRLLTGELRQGALIGVMADAVAKAADVPAARVRRAAMLAG
DLPAVAAAVLAGGDAALARFTLQVGRPVGPMLAQTATGVADALDRLGGTAVFEAKLDGARVQIHRRGSDVSVYTRSLDDV
TARLPEVVEAALALPVTDLIADAEAIALRPDGRPHRFQVTASRFGRAAARATQPLSVFMFDLLHVDGADLLDQPTSDRVR
VLDDLVPAAHRVDRLVTDDGAAAQRFLEATLAAGHEGVMAKSPNAPYEAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRT
GKLSNIHLGARDPATGGFVMLGKTFKGMTDAMLDWQTARFLELADPAAQPATSGRDPTDGHTVKVRPEQVVEIAFDGVQG
STRYPGGMALRFARVLRYRDDKSPAEADTVDTVRAFYEHG

Sequences:

>Translated_520_residues
MLLVDVATASVDVGAMSSRLAKTARIADLLSRAGTEQDARLVAVTVAWLSGELPQRQIGVGWAALRSLPAPAAAPTLTVT
AVDAVFSEIGAVAGKGSQARRAGLIAELFAAATDVEQTFLRRLLTGELRQGALIGVMADAVAKAADVPAARVRRAAMLAG
DLPAVAAAVLAGGDAALARFTLQVGRPVGPMLAQTATGVADALDRLGGTAVFEAKLDGARVQIHRRGSDVSVYTRSLDDV
TARLPEVVEAALALPVTDLIADAEAIALRPDGRPHRFQVTASRFGRAAARATQPLSVFMFDLLHVDGADLLDQPTSDRVR
VLDDLVPAAHRVDRLVTDDGAAAQRFLEATLAAGHEGVMAKSPNAPYEAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRT
GKLSNIHLGARDPATGGFVMLGKTFKGMTDAMLDWQTARFLELADPAAQPATSGRDPTDGHTVKVRPEQVVEIAFDGVQG
STRYPGGMALRFARVLRYRDDKSPAEADTVDTVRAFYEHG
>Mature_520_residues
MLLVDVATASVDVGAMSSRLAKTARIADLLSRAGTEQDARLVAVTVAWLSGELPQRQIGVGWAALRSLPAPAAAPTLTVT
AVDAVFSEIGAVAGKGSQARRAGLIAELFAAATDVEQTFLRRLLTGELRQGALIGVMADAVAKAADVPAARVRRAAMLAG
DLPAVAAAVLAGGDAALARFTLQVGRPVGPMLAQTATGVADALDRLGGTAVFEAKLDGARVQIHRRGSDVSVYTRSLDDV
TARLPEVVEAALALPVTDLIADAEAIALRPDGRPHRFQVTASRFGRAAARATQPLSVFMFDLLHVDGADLLDQPTSDRVR
VLDDLVPAAHRVDRLVTDDGAAAQRFLEATLAAGHEGVMAKSPNAPYEAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRT
GKLSNIHLGARDPATGGFVMLGKTFKGMTDAMLDWQTARFLELADPAAQPATSGRDPTDGHTVKVRPEQVVEIAFDGVQG
STRYPGGMALRFARVLRYRDDKSPAEADTVDTVRAFYEHG

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=505, Percent_Identity=27.7227722772277, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI46255052, Length=471, Percent_Identity=24.416135881104, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI23199993, Length=471, Percent_Identity=24.416135881104, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI148539894, Length=471, Percent_Identity=24.416135881104, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI73747829, Length=502, Percent_Identity=26.4940239043825, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI73747844, Length=502, Percent_Identity=26.4940239043825, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71985269, Length=497, Percent_Identity=28.3702213279678, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6320038, Length=491, Percent_Identity=28.5132382892057, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324578, Length=258, Percent_Identity=26.3565891472868, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24762424, Length=503, Percent_Identity=27.8330019880716, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI85815841, Length=459, Percent_Identity=28.1045751633987, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_MYCSJ (A3PXE2)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001070060.1
- ProteinModelPortal:   A3PXE2
- SMR:   A3PXE2
- STRING:   A3PXE2
- EnsemblBacteria:   EBMYCT00000051846
- GeneID:   4877503
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_1780
- eggNOG:   COG1793
- GeneTree:   EBGT00050000016312
- HOGENOM:   HBG570821
- OMA:   GRPRPFQ
- PhylomeDB:   A3PXE2
- ProtClustDB:   PRK03180
- BioCyc:   MSP164757:MJLS_1780-MONOMER
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 54706; Mature: 54706

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 215-215 BINDING 213-213 BINDING 220-220 BINDING 235-235 BINDING 264-264 BINDING 300-300 BINDING 372-372 BINDING 378-378

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLVDVATASVDVGAMSSRLAKTARIADLLSRAGTEQDARLVAVTVAWLSGELPQRQIGV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHHCC
GWAALRSLPAPAAAPTLTVTAVDAVFSEIGAVAGKGSQARRAGLIAELFAAATDVEQTFL
HHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRLLTGELRQGALIGVMADAVAKAADVPAARVRRAAMLAGDLPAVAAAVLAGGDAALARF
HHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHH
TLQVGRPVGPMLAQTATGVADALDRLGGTAVFEAKLDGARVQIHRRGSDVSVYTRSLDDV
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCEEEEEHHHHHH
TARLPEVVEAALALPVTDLIADAEAIALRPDGRPHRFQVTASRFGRAAARATQPLSVFMF
HHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHH
DLLHVDGADLLDQPTSDRVRVLDDLVPAAHRVDRLVTDDGAAAQRFLEATLAAGHEGVMA
HHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEE
KSPNAPYEAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRTGKLSNIHLGARDPATGGFVM
CCCCCCHHCCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
LGKTFKGMTDAMLDWQTARFLELADPAAQPATSGRDPTDGHTVKVRPEQVVEIAFDGVQG
ECCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCC
STRYPGGMALRFARVLRYRDDKSPAEADTVDTVRAFYEHG
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLVDVATASVDVGAMSSRLAKTARIADLLSRAGTEQDARLVAVTVAWLSGELPQRQIGV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHHCC
GWAALRSLPAPAAAPTLTVTAVDAVFSEIGAVAGKGSQARRAGLIAELFAAATDVEQTFL
HHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRLLTGELRQGALIGVMADAVAKAADVPAARVRRAAMLAGDLPAVAAAVLAGGDAALARF
HHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHH
TLQVGRPVGPMLAQTATGVADALDRLGGTAVFEAKLDGARVQIHRRGSDVSVYTRSLDDV
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCEEEEEHHHHHH
TARLPEVVEAALALPVTDLIADAEAIALRPDGRPHRFQVTASRFGRAAARATQPLSVFMF
HHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHH
DLLHVDGADLLDQPTSDRVRVLDDLVPAAHRVDRLVTDDGAAAQRFLEATLAAGHEGVMA
HHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEE
KSPNAPYEAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRTGKLSNIHLGARDPATGGFVM
CCCCCCHHCCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
LGKTFKGMTDAMLDWQTARFLELADPAAQPATSGRDPTDGHTVKVRPEQVVEIAFDGVQG
ECCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCC
STRYPGGMALRFARVLRYRDDKSPAEADTVDTVRAFYEHG
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA