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The map label for this gene is yngI [H]

Identifier: 119867872

GI number: 119867872

Start: 1945698

End: 1947347

Strand: Reverse

Name: yngI [H]

Synonym: Mkms_1833

Alternate gene names: 119867872

Gene position: 1947347-1945698 (Counterclockwise)

Preceding gene: 119867873

Following gene: 119867868

Centisome position: 33.94

GC content: 70.36

Gene sequence:

>1650_bases
ATGCAGTTCACGATGCCCCAGGTGGCCGACGCGGTCGCCGAGGTGCTCCCCGACCGCGAACTCGTCGTCCAGGGTGGACA
GCGCCACACGTACGCCGACATCGTCGCGCGGTCGAATCGGCTCGCCTCGTACCTCCACGGCGCAGGGCTCGGATGCCACA
CCGAACGGTCGGAACTCGGCGGCCACGAAACCGGCCAGGACCTACTCGGGATCTACGCCCACAACAGCACCGCCTACGTC
GAGGGCCTGCTCGGGGCCTTCCGCGCGCGGGTCGCCCCGTTCAACGTCAACTACCGCTACGTACGCGACGAGTTGCGCTA
CTTGCTCGACGACAGCGGGGCCACCGCACTGCTCTACCACGCATGCTTCGCGCCCCGCGTCGCCGAGGTGCTGCCCGACC
TGCCGCGGCTGCGCACCCTGATCCAGGTCGCCGACGGCTCCGGAAACGAACTCCTCCCGGGCGCAGTCGATTACGAGTCG
ACCGTCGCGTCAGGGCCATCCACCCCGCCACCGGTGCAGCCCTCCCCCGATGACCTCTACGTCCTCTACACCGGCGGCAC
CACCGGCATGCCGAAGGGTGTCCTGTGGCGTCAGCACGACATCTTCATGACCGCATTCGGTGGCCGCTCGCTGTACGGCG
GCGACACCGTGACCTCCCTCGAGGAGGTTGCGCAGCGGGCGGCGGCCAACCCCGGGACCAAACTGCTGATCCTGCCGCCG
CTGATGCACGGCGCCGCGCAGTGGGCCGTGATGACCGCGTTGACGACCGGTCAGACGGTGGTGTTCACCTCGGTCGTCGA
CCGGCTCGACGCGCAGGACGCACTGGACACGATCGCCCGCGAGCAGGCCTCGGTCGTGACGGTGGTCGGCGATGCGATGG
CCCGCCCACTCGCCGACGCCGTCGAGAAAGCCCGCCGGAGCGGGGAACCCGACATCTCCTCGCTGGCCGTCGTCGCCAAC
GGCGGGGCGTTCCTCACCCCGACCACCAAACAACGGCTGATCGACCTGCTGCCCAACGCGATGGTGGTCGACGGAGTCGG
GTCCTCGGAGACGGGCGCCCAGATGACCCACCTGTCGATGAACGGCGCCGTCGCCACCGGCAGGTTCAACGCCGGACCGG
ACACCTGCGTGATCGCCGAGGATCTCGGCTCGGTGCTGCCGCCGGGGCACGACGGACTCGGCTGGCTCGCACAGCGCGGC
TACGTACCGCTGGGGTACAAGGGCGATGCGGCGAAGACCGCGAGGACCTTCCCCGTCGTCGACGGCGTGCGGTACGCGGT
GCCCGGTGACCGCGCCCGACACCTCGCGGGCGGTGAGATCGAACTGCTCGGCCGCGATTCGGTGACCATCAACTCCGGGG
GCGAGAAGATCTTCGCCGAGGAGGTCGAGGCCGCGCTCGCCTCACACCCCGCAGTCGAGGACGTGCTGGTGGCCGGCCGG
CCGAGCGACAGGTGGGGTCAGGAGGTGGTGGCGGTGGTCGCACTCGCCGAGGGCGCCACGGCCGACGCCGACGAACTGAT
CACCCACGCGGCCAACAGCATTGCGCGCTACAAGCTGCCCAAGGCGGTGGTGTTCCGGGCGGCGATCCAGCGCAGCCCGG
CGGGCAAGGCCGACTACCGCTGGGCCCGCGAACAGGCGATCGGCAGCTGA

Upstream 100 bases:

>100_bases
CGACCGAGTACGGCGACGCCGTCATCGACAACACACTGTCGCTGTTGGCGACGATCACCACCACAGAGGGTCTGCTGCAG
ACCTGGAAGCAGGAGGCATG

Downstream 100 bases:

>100_bases
CCTCAGCGCAGCACCCGGACCGGCACGTTCTGCCAGCCTGCGACGTTCTGCATGTTCACCCGTCGGCAGCCCGCCCAGTC
CACCTCGTAGGCGGGCATGA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MQFTMPQVADAVAEVLPDRELVVQGGQRHTYADIVARSNRLASYLHGAGLGCHTERSELGGHETGQDLLGIYAHNSTAYV
EGLLGAFRARVAPFNVNYRYVRDELRYLLDDSGATALLYHACFAPRVAEVLPDLPRLRTLIQVADGSGNELLPGAVDYES
TVASGPSTPPPVQPSPDDLYVLYTGGTTGMPKGVLWRQHDIFMTAFGGRSLYGGDTVTSLEEVAQRAAANPGTKLLILPP
LMHGAAQWAVMTALTTGQTVVFTSVVDRLDAQDALDTIAREQASVVTVVGDAMARPLADAVEKARRSGEPDISSLAVVAN
GGAFLTPTTKQRLIDLLPNAMVVDGVGSSETGAQMTHLSMNGAVATGRFNAGPDTCVIAEDLGSVLPPGHDGLGWLAQRG
YVPLGYKGDAAKTARTFPVVDGVRYAVPGDRARHLAGGEIELLGRDSVTINSGGEKIFAEEVEAALASHPAVEDVLVAGR
PSDRWGQEVVAVVALAEGATADADELITHAANSIARYKLPKAVVFRAAIQRSPAGKADYRWAREQAIGS

Sequences:

>Translated_549_residues
MQFTMPQVADAVAEVLPDRELVVQGGQRHTYADIVARSNRLASYLHGAGLGCHTERSELGGHETGQDLLGIYAHNSTAYV
EGLLGAFRARVAPFNVNYRYVRDELRYLLDDSGATALLYHACFAPRVAEVLPDLPRLRTLIQVADGSGNELLPGAVDYES
TVASGPSTPPPVQPSPDDLYVLYTGGTTGMPKGVLWRQHDIFMTAFGGRSLYGGDTVTSLEEVAQRAAANPGTKLLILPP
LMHGAAQWAVMTALTTGQTVVFTSVVDRLDAQDALDTIAREQASVVTVVGDAMARPLADAVEKARRSGEPDISSLAVVAN
GGAFLTPTTKQRLIDLLPNAMVVDGVGSSETGAQMTHLSMNGAVATGRFNAGPDTCVIAEDLGSVLPPGHDGLGWLAQRG
YVPLGYKGDAAKTARTFPVVDGVRYAVPGDRARHLAGGEIELLGRDSVTINSGGEKIFAEEVEAALASHPAVEDVLVAGR
PSDRWGQEVVAVVALAEGATADADELITHAANSIARYKLPKAVVFRAAIQRSPAGKADYRWAREQAIGS
>Mature_549_residues
MQFTMPQVADAVAEVLPDRELVVQGGQRHTYADIVARSNRLASYLHGAGLGCHTERSELGGHETGQDLLGIYAHNSTAYV
EGLLGAFRARVAPFNVNYRYVRDELRYLLDDSGATALLYHACFAPRVAEVLPDLPRLRTLIQVADGSGNELLPGAVDYES
TVASGPSTPPPVQPSPDDLYVLYTGGTTGMPKGVLWRQHDIFMTAFGGRSLYGGDTVTSLEEVAQRAAANPGTKLLILPP
LMHGAAQWAVMTALTTGQTVVFTSVVDRLDAQDALDTIAREQASVVTVVGDAMARPLADAVEKARRSGEPDISSLAVVAN
GGAFLTPTTKQRLIDLLPNAMVVDGVGSSETGAQMTHLSMNGAVATGRFNAGPDTCVIAEDLGSVLPPGHDGLGWLAQRG
YVPLGYKGDAAKTARTFPVVDGVRYAVPGDRARHLAGGEIELLGRDSVTINSGGEKIFAEEVEAALASHPAVEDVLVAGR
PSDRWGQEVVAVVALAEGATADADELITHAANSIARYKLPKAVVFRAAIQRSPAGKADYRWAREQAIGS

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=593, Percent_Identity=25.8010118043845, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI38505220, Length=462, Percent_Identity=24.6753246753247, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI115511026, Length=460, Percent_Identity=25, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI187761345, Length=566, Percent_Identity=25.9717314487633, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI187761343, Length=566, Percent_Identity=25.9717314487633, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI58082049, Length=477, Percent_Identity=23.6897274633124, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788107, Length=561, Percent_Identity=24.5989304812834, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693145, Length=375, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786810, Length=413, Percent_Identity=26.1501210653753, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=487, Percent_Identity=24.2299794661191, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=565, Percent_Identity=24.9557522123894, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319699, Length=567, Percent_Identity=24.5149911816578, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24581924, Length=570, Percent_Identity=25.7894736842105, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI18859661, Length=492, Percent_Identity=24.7967479674797, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355181, Length=532, Percent_Identity=23.8721804511278, Blast_Score=83, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 57991; Mature: 57991

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFTMPQVADAVAEVLPDRELVVQGGQRHTYADIVARSNRLASYLHGAGLGCHTERSELG
CCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
GHETGQDLLGIYAHNSTAYVEGLLGAFRARVAPFNVNYRYVRDELRYLLDDSGATALLYH
CCCCCCHHEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
ACFAPRVAEVLPDLPRLRTLIQVADGSGNELLPGAVDYESTVASGPSTPPPVQPSPDDLY
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEE
VLYTGGTTGMPKGVLWRQHDIFMTAFGGRSLYGGDTVTSLEEVAQRAAANPGTKLLILPP
EEEECCCCCCCCCCEEEECCEEEEEECCCEECCCCHHHHHHHHHHHHCCCCCCEEEEECC
LMHGAAQWAVMTALTTGQTVVFTSVVDRLDAQDALDTIAREQASVVTVVGDAMARPLADA
HHHHHHHHHHHHHHHCCCEEHHHHHHHHHCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHH
VEKARRSGEPDISSLAVVANGGAFLTPTTKQRLIDLLPNAMVVDGVGSSETGAQMTHLSM
HHHHHHCCCCCHHEEEEEECCCEEECCCHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEC
NGAVATGRFNAGPDTCVIAEDLGSVLPPGHDGLGWLAQRGYVPLGYKGDAAKTARTFPVV
CCEEEECCCCCCCCCEEEHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DGVRYAVPGDRARHLAGGEIELLGRDSVTINSGGEKIFAEEVEAALASHPAVEDVLVAGR
CCEEEECCCCHHHCCCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHCCCCHHHHEEECC
PSDRWGQEVVAVVALAEGATADADELITHAANSIARYKLPKAVVFRAAIQRSPAGKADYR
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHH
WAREQAIGS
HHHHHHCCC
>Mature Secondary Structure
MQFTMPQVADAVAEVLPDRELVVQGGQRHTYADIVARSNRLASYLHGAGLGCHTERSELG
CCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
GHETGQDLLGIYAHNSTAYVEGLLGAFRARVAPFNVNYRYVRDELRYLLDDSGATALLYH
CCCCCCHHEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
ACFAPRVAEVLPDLPRLRTLIQVADGSGNELLPGAVDYESTVASGPSTPPPVQPSPDDLY
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEE
VLYTGGTTGMPKGVLWRQHDIFMTAFGGRSLYGGDTVTSLEEVAQRAAANPGTKLLILPP
EEEECCCCCCCCCCEEEECCEEEEEECCCEECCCCHHHHHHHHHHHHCCCCCCEEEEECC
LMHGAAQWAVMTALTTGQTVVFTSVVDRLDAQDALDTIAREQASVVTVVGDAMARPLADA
HHHHHHHHHHHHHHHCCCEEHHHHHHHHHCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHH
VEKARRSGEPDISSLAVVANGGAFLTPTTKQRLIDLLPNAMVVDGVGSSETGAQMTHLSM
HHHHHHCCCCCHHEEEEEECCCEEECCCHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEC
NGAVATGRFNAGPDTCVIAEDLGSVLPPGHDGLGWLAQRGYVPLGYKGDAAKTARTFPVV
CCEEEECCCCCCCCCEEEHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DGVRYAVPGDRARHLAGGEIELLGRDSVTINSGGEKIFAEEVEAALASHPAVEDVLVAGR
CCEEEECCCCHHHCCCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHCCCCHHHHEEECC
PSDRWGQEVVAVVALAEGATADADELITHAANSIARYKLPKAVVFRAAIQRSPAGKADYR
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHH
WAREQAIGS
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]