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The map label for this gene is yagI [H]

Identifier: 119867691

GI number: 119867691

Start: 1761734

End: 1762627

Strand: Reverse

Name: yagI [H]

Synonym: Mkms_1646

Alternate gene names: 119867691

Gene position: 1762627-1761734 (Counterclockwise)

Preceding gene: 119867697

Following gene: 119867690

Centisome position: 30.72

GC content: 62.42

Gene sequence:

>894_bases
GTGCAGGAGGGCGACTTTTCGTCCGCCGGCACCCGAGTCGTCGCGATTCGTCGAGCTGCATTCGGACCTCTCCGCGGTAC
GCTCTCTGTCATGCAGAAACCTCCACTCGCGGGCATGGAGCATGACAGCACAGGGAAGCCCCCGCAATATCCGATCGAGT
CTGTCGACAACGCTCTTCGCCTACTCCTCCTCCTAGGAGAGCGCTCTGAGATCCGGCTCACGGACGCTAGCGAGTACCTG
CGCGTCGCTTCGTCGACCGCGCATCGCCTTCTGTCGATGCTTCAGTACCGCGGGTTCGTCCGCCAAGATCCGGCCAGCAA
GGCGTACCAGCCCGGACCGTCCCTGACAAACGTAGCGTTCGCTGTCCACTCCAGGATGGACATTCCACGCACGGCCGCCC
CGATTCTGCGCCAGCTTGTCGATACGCTCCAAGAAACCGTGCACGTAGGAACGTTGGACGGCAGACTGGTTCGCTTCATC
GCGGCACTCGAAAGCCCTTCTGCCGTTCGGGTGATCTCGCGTTTGGGTAGCACGCGTCCTGCACACTGCACGTCGACCGG
TAAAGTCCTGCTCGCCGGCCTATCGGACGACGAGCTCGACCAGTTGTACCCCGACCCACATTTGGAGCCACTCACCGAGC
ACTCCGTGCGCACCCTCACCGAACTGCGCGCTCAATTGGCAGAGGTCAGGAAAAGCGGGTTCGCGATCAGCAGGCAGGAA
AGTGAGGAAGGCGTCGCGTCTGTCGCCGTGGCCGTACCCCAAAGTGGTTCCGGGATAAGGCTTGCACTCAACGCGTCAGC
GCCGAGTTATCGATTCCGGCCCTCCGATGTTCGCAAAGCTTGCAGTGCCCTGACAGACGCCGCCGCCGAGCTAGCCCGCA
ATCTCGGCTCATGA

Upstream 100 bases:

>100_bases
GTGCTGCTCGTCGACGACGACACCGCCGCGTGCGCCAAGAAGCAAGTCGACGGTCCCTATCTACCCGCCGCCGCCTGTCA
GACAAACGGCCCAGGTTTGT

Downstream 100 bases:

>100_bases
CATCCGAAAAATCGGAAGCCGACACTGAGATGCCCGGCGGTGCCCCGGACCTGCCTGCCTTTATTGCGACCATCGATGCA
TTCCTACGCCTGGAGAAGCG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MQEGDFSSAGTRVVAIRRAAFGPLRGTLSVMQKPPLAGMEHDSTGKPPQYPIESVDNALRLLLLLGERSEIRLTDASEYL
RVASSTAHRLLSMLQYRGFVRQDPASKAYQPGPSLTNVAFAVHSRMDIPRTAAPILRQLVDTLQETVHVGTLDGRLVRFI
AALESPSAVRVISRLGSTRPAHCTSTGKVLLAGLSDDELDQLYPDPHLEPLTEHSVRTLTELRAQLAEVRKSGFAISRQE
SEEGVASVAVAVPQSGSGIRLALNASAPSYRFRPSDVRKACSALTDAAAELARNLGS

Sequences:

>Translated_297_residues
MQEGDFSSAGTRVVAIRRAAFGPLRGTLSVMQKPPLAGMEHDSTGKPPQYPIESVDNALRLLLLLGERSEIRLTDASEYL
RVASSTAHRLLSMLQYRGFVRQDPASKAYQPGPSLTNVAFAVHSRMDIPRTAAPILRQLVDTLQETVHVGTLDGRLVRFI
AALESPSAVRVISRLGSTRPAHCTSTGKVLLAGLSDDELDQLYPDPHLEPLTEHSVRTLTELRAQLAEVRKSGFAISRQE
SEEGVASVAVAVPQSGSGIRLALNASAPSYRFRPSDVRKACSALTDAAAELARNLGS
>Mature_297_residues
MQEGDFSSAGTRVVAIRRAAFGPLRGTLSVMQKPPLAGMEHDSTGKPPQYPIESVDNALRLLLLLGERSEIRLTDASEYL
RVASSTAHRLLSMLQYRGFVRQDPASKAYQPGPSLTNVAFAVHSRMDIPRTAAPILRQLVDTLQETVHVGTLDGRLVRFI
AALESPSAVRVISRLGSTRPAHCTSTGKVLLAGLSDDELDQLYPDPHLEPLTEHSVRTLTELRAQLAEVRKSGFAISRQE
SEEGVASVAVAVPQSGSGIRLALNASAPSYRFRPSDVRKACSALTDAAAELARNLGS

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=246, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788131, Length=245, Percent_Identity=28.9795918367347, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1786715, Length=225, Percent_Identity=29.7777777777778, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789999, Length=228, Percent_Identity=28.0701754385965, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082359, Length=253, Percent_Identity=25.296442687747, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32014; Mature: 32014

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEGDFSSAGTRVVAIRRAAFGPLRGTLSVMQKPPLAGMEHDSTGKPPQYPIESVDNALR
CCCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LLLLLGERSEIRLTDASEYLRVASSTAHRLLSMLQYRGFVRQDPASKAYQPGPSLTNVAF
HHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHH
AVHSRMDIPRTAAPILRQLVDTLQETVHVGTLDGRLVRFIAALESPSAVRVISRLGSTRP
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCC
AHCTSTGKVLLAGLSDDELDQLYPDPHLEPLTEHSVRTLTELRAQLAEVRKSGFAISRQE
CCCCCCCCEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCH
SEEGVASVAVAVPQSGSGIRLALNASAPSYRFRPSDVRKACSALTDAAAELARNLGS
HHCCHHEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQEGDFSSAGTRVVAIRRAAFGPLRGTLSVMQKPPLAGMEHDSTGKPPQYPIESVDNALR
CCCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LLLLLGERSEIRLTDASEYLRVASSTAHRLLSMLQYRGFVRQDPASKAYQPGPSLTNVAF
HHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHH
AVHSRMDIPRTAAPILRQLVDTLQETVHVGTLDGRLVRFIAALESPSAVRVISRLGSTRP
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCC
AHCTSTGKVLLAGLSDDELDQLYPDPHLEPLTEHSVRTLTELRAQLAEVRKSGFAISRQE
CCCCCCCCEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCH
SEEGVASVAVAVPQSGSGIRLALNASAPSYRFRPSDVRKACSALTDAAAELARNLGS
HHCCHHEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]