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The map label for this gene is tgs3 [H]

Identifier: 119867423

GI number: 119867423

Start: 1472592

End: 1473998

Strand: Direct

Name: tgs3 [H]

Synonym: Mkms_1373

Alternate gene names: 119867423

Gene position: 1472592-1473998 (Clockwise)

Preceding gene: 119867421

Following gene: 119867424

Centisome position: 25.67

GC content: 68.87

Gene sequence:

>1407_bases
ATGGTGACCCGGCTGTCGGCGTCGGATGCGGAGTTCTTCCGGCTGGAGGACACCTCGACCCCGATGTACGTCGGCACGTT
GTCGATCCTGCGCAAACCGCGCAGCGGTCTGAGCTACGAGACCCTGCTGCACACCGTCGAGCAGCGGCTGCCCCAGATCC
CGCGGTACCGGCAGAAGGTCCGCGAAGTGACCATGGGCCTCGCGCGGCCGGTCTGGGTCGACGACCGCGACTTCGACATC
ACCTACCACATCCGTCGCTCGGCCCTCCCGTCTCCGGGCAGCGACGCCCAGCTGCTCGACCTGGTCGCCCGCCTCGGCTC
GCGGCCGCTCGACAAGTCCCGGCCGCTGTGGGAGATGTACCTGGTCGAGGGGTTGGCGAAGAACCGCGTCGCGATCTACA
CGAAGAGCCATCAGGCGCTGGTCAACGGTATGACGGCGCTGGAGATCGGCCACGTCATCGCCGACCGCACCCAGAAACCG
CCCGACTTCGGTGAGGACATCTGGATCCCCGCGCGCGAGCCCAGCGACCGGGCGTTGCTGCTCGGCGCGGTCGGTGAATG
GGTCACCCGCCCCGCCGAGCAGGTCGCCGCGGTCAGGTCGGTGGTGACCGACCTCGCCAACGCCGAACAACTCGTCGCCG
CCGGCCGCCGCGTCGCCGACATCGCCCGCACCCTCGCCCGCGGCACCGCGCCGAGCAGCCCGCTCAACACCACCGTGTCG
CGCAACCGGCGATTCTCGGTGGCCGGGCACCGTCTCGAGGACTACCGCACCGTGCGGGCACGCTACGACTGCGACATCAA
CGACGTGGTGCTCGCCGTCGTCGCCGGGGCCCTGCGCAACTGGCTGCTCTCCCGCGGTGAACCGGTCACCCCGACCACCA
CCGTGCGGGCGATGGCACCGATGTCGGTCTACCCCGACGCCGACATCGACACGTCCGGACCGGGTCAGGCGATCAGCCAG
GTGTCGCCGTTCCTGGTGGACCTGCCGGTGGGGGAGGGCAATGCGGTGGTGCGGCTGTCGCAGATCGCCCACGCCACCGA
GACGCACCCGACCGCGCCCAGCCTGGTGGATGCCCGCACGATCGTCACCCTGTCCGGGTTTGCGCCACCGACGTTGCACG
CCATGGGAATTCGGGTGGCGACCACCTTCCCCGCGCGACAGTTCAACCTGCTCATCACCAACGTGCCCGGTGCGCAGAAA
CAGATGTTCGTCGCCGGCACGAAGTTGTTGGAGACCTATGCGGTGCCACCGCTGCTACACAACCAGGTGCTCGCGATCGG
GGTCACCTCCTACAACGGCGAGCTGTACTTCGGCATCAACGCCGACCGCGACGCGATGAGCGATGTCGACGTCCTGCCCG
GTCTGCTGTCGGAGTCTCTCGAGGAACTCCTGGAAGCCGCTCGGTGA

Upstream 100 bases:

>100_bases
CGGTTCACGGGAGGTCCAGAGCACCCGTGATGCGGCTGTACAGACGGGGCCGCTACCGTGGCGGGGAACTCACGGGATTC
GACGAAAGGGGAGGCGTCGG

Downstream 100 bases:

>100_bases
CCGGATCGTGGCCGACCTGGGATTGACGAGAATCCCTGCGCGGGTTCTCATGGTCGGACCATGACGGTCAGTTCAGAGAA
GGACAACGAGAAACACCGCC

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: TGS3; Probable triacylglycerol synthase tgs3 [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MVTRLSASDAEFFRLEDTSTPMYVGTLSILRKPRSGLSYETLLHTVEQRLPQIPRYRQKVREVTMGLARPVWVDDRDFDI
TYHIRRSALPSPGSDAQLLDLVARLGSRPLDKSRPLWEMYLVEGLAKNRVAIYTKSHQALVNGMTALEIGHVIADRTQKP
PDFGEDIWIPAREPSDRALLLGAVGEWVTRPAEQVAAVRSVVTDLANAEQLVAAGRRVADIARTLARGTAPSSPLNTTVS
RNRRFSVAGHRLEDYRTVRARYDCDINDVVLAVVAGALRNWLLSRGEPVTPTTTVRAMAPMSVYPDADIDTSGPGQAISQ
VSPFLVDLPVGEGNAVVRLSQIAHATETHPTAPSLVDARTIVTLSGFAPPTLHAMGIRVATTFPARQFNLLITNVPGAQK
QMFVAGTKLLETYAVPPLLHNQVLAIGVTSYNGELYFGINADRDAMSDVDVLPGLLSESLEELLEAAR

Sequences:

>Translated_468_residues
MVTRLSASDAEFFRLEDTSTPMYVGTLSILRKPRSGLSYETLLHTVEQRLPQIPRYRQKVREVTMGLARPVWVDDRDFDI
TYHIRRSALPSPGSDAQLLDLVARLGSRPLDKSRPLWEMYLVEGLAKNRVAIYTKSHQALVNGMTALEIGHVIADRTQKP
PDFGEDIWIPAREPSDRALLLGAVGEWVTRPAEQVAAVRSVVTDLANAEQLVAAGRRVADIARTLARGTAPSSPLNTTVS
RNRRFSVAGHRLEDYRTVRARYDCDINDVVLAVVAGALRNWLLSRGEPVTPTTTVRAMAPMSVYPDADIDTSGPGQAISQ
VSPFLVDLPVGEGNAVVRLSQIAHATETHPTAPSLVDARTIVTLSGFAPPTLHAMGIRVATTFPARQFNLLITNVPGAQK
QMFVAGTKLLETYAVPPLLHNQVLAIGVTSYNGELYFGINADRDAMSDVDVLPGLLSESLEELLEAAR
>Mature_468_residues
MVTRLSASDAEFFRLEDTSTPMYVGTLSILRKPRSGLSYETLLHTVEQRLPQIPRYRQKVREVTMGLARPVWVDDRDFDI
TYHIRRSALPSPGSDAQLLDLVARLGSRPLDKSRPLWEMYLVEGLAKNRVAIYTKSHQALVNGMTALEIGHVIADRTQKP
PDFGEDIWIPAREPSDRALLLGAVGEWVTRPAEQVAAVRSVVTDLANAEQLVAAGRRVADIARTLARGTAPSSPLNTTVS
RNRRFSVAGHRLEDYRTVRARYDCDINDVVLAVVAGALRNWLLSRGEPVTPTTTVRAMAPMSVYPDADIDTSGPGQAISQ
VSPFLVDLPVGEGNAVVRLSQIAHATETHPTAPSLVDARTIVTLSGFAPPTLHAMGIRVATTFPARQFNLLITNVPGAQK
QMFVAGTKLLETYAVPPLLHNQVLAIGVTSYNGELYFGINADRDAMSDVDVLPGLLSESLEELLEAAR

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity to produce wax esters [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 51179; Mature: 51179

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTRLSASDAEFFRLEDTSTPMYVGTLSILRKPRSGLSYETLLHTVEQRLPQIPRYRQKV
CCCCCCCCCCCEEEEECCCCCEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
REVTMGLARPVWVDDRDFDITYHIRRSALPSPGSDAQLLDLVARLGSRPLDKSRPLWEMY
HHHHHHHCCCEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
LVEGLAKNRVAIYTKSHQALVNGMTALEIGHVIADRTQKPPDFGEDIWIPAREPSDRALL
HHHHHHCCEEEEEECCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCEEE
LGAVGEWVTRPAEQVAAVRSVVTDLANAEQLVAAGRRVADIARTLARGTAPSSPLNTTVS
EEHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHH
RNRRFSVAGHRLEDYRTVRARYDCDINDVVLAVVAGALRNWLLSRGEPVTPTTTVRAMAP
CCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECC
MSVYPDADIDTSGPGQAISQVSPFLVDLPVGEGNAVVRLSQIAHATETHPTAPSLVDART
CEECCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHCCCCCCCCHHCCEE
IVTLSGFAPPTLHAMGIRVATTFPARQFNLLITNVPGAQKQMFVAGTKLLETYAVPPLLH
EEEECCCCCCCHHHHCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHH
NQVLAIGVTSYNGELYFGINADRDAMSDVDVLPGLLSESLEELLEAAR
CCEEEEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTRLSASDAEFFRLEDTSTPMYVGTLSILRKPRSGLSYETLLHTVEQRLPQIPRYRQKV
CCCCCCCCCCCEEEEECCCCCEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
REVTMGLARPVWVDDRDFDITYHIRRSALPSPGSDAQLLDLVARLGSRPLDKSRPLWEMY
HHHHHHHCCCEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
LVEGLAKNRVAIYTKSHQALVNGMTALEIGHVIADRTQKPPDFGEDIWIPAREPSDRALL
HHHHHHCCEEEEEECCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCEEE
LGAVGEWVTRPAEQVAAVRSVVTDLANAEQLVAAGRRVADIARTLARGTAPSSPLNTTVS
EEHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHH
RNRRFSVAGHRLEDYRTVRARYDCDINDVVLAVVAGALRNWLLSRGEPVTPTTTVRAMAP
CCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECC
MSVYPDADIDTSGPGQAISQVSPFLVDLPVGEGNAVVRLSQIAHATETHPTAPSLVDART
CEECCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHCCCCCCCCHHCCEE
IVTLSGFAPPTLHAMGIRVATTFPARQFNLLITNVPGAQKQMFVAGTKLLETYAVPPLLH
EEEECCCCCCCHHHHCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHH
NQVLAIGVTSYNGELYFGINADRDAMSDVDVLPGLLSESLEELLEAAR
CCEEEEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]