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The map label for this gene is mdeA [H]

Identifier: 119867275

GI number: 119867275

Start: 1310280

End: 1311587

Strand: Reverse

Name: mdeA [H]

Synonym: Mkms_1225

Alternate gene names: 119867275

Gene position: 1311587-1310280 (Counterclockwise)

Preceding gene: 119867277

Following gene: 119867274

Centisome position: 22.86

GC content: 67.43

Gene sequence:

>1308_bases
ATGAGCACGCCAGAAGACCTGATCGCCAATTGGTCGTTCGAGACCAAGCAGGTCCACGCCGGCCAGACGCCCGACGCGGC
GACCAACGCCCGCGCCCTGCCGATCTACCAGACCACCTCGTACACCTTCCGCGACACCGACCACGCCGCGGCGCTGTTCG
GGCTGGCCGAGACCGGCAACATCTACACGCGGATCATGAACCCGACGACCGACGTCGTCGAACAGCGCATCGCCGCGCTC
GAAGGTGGTGTGGCGGCGCTGTTCCTGTCGTCCGGACAGGCCGCGGAGACGTTCGCGATCCTCAACATCGCCAACGCGGG
CGATCACATCGTGTCCTCCCCGCGCCTCTACGGCGGCACGTACAACCTGTTCCACTACACGCTGCCCAAACTCGGCATCG
ACGTCAGCTTCGTGGAGAATCCGGACGACCTCGACTCGTGGCGGGCCGCGGTGCGCCCCAACACCAAGGCGTTCTTCGGT
GAGACGATCTCCAATCCGCAGATCGATGTGCTCGACATCCCCAATGTCGCCGCCGTCGCCCACGAGGCCGGTGTGCCGCT
GATCGTCGACAACACCATCGCCACGCCGTACCTGATCCAGCCGTTGAGCCACGGCGCCGACATCGTCGTGCACTCGGCCA
CCAAGTACCTCGGCGGACACGGCAACGCGATCGCCGGTGTGATCGTCGACGGCGGCACGTTCGACTGGACCAACGGGCGC
TTCCCGGGTTTCACCACCCCGGATCCGAGCTACCACGGGGTGGTGTTCGCCGACCTCGGCGCACCGGCCTATGCGCTCAA
GGCGCGTGTGCAGTTGCTGCGCGACCTCGGTTCGGCCGCCGCACCGTTCAACGCGTTCCTGATCGCCCAGGGCCTGGAGA
CGTTGAGTCTGCGCGTTGAACGGCATGTGGCCAACGCGCAGAAGGTCGCCGAGTACCTGGCCGGCCACGACGACGTGCTG
TCGGTGAACTACGCCGGTCTGCCCAGCTCACCGTGGTATGAGCTCGGACAGCGGTTGGCGCCCAAGGGAACCGGCGCGGT
GCTGGCGTTCGAGCTGGCCGGCGGCGTCGATGCGGGCAAGGCGTTCGTCAACGCGCTCACGCTGCACAGCCATGTCGCCA
ACATCGGTGACGTGCGCTCCCTGGTGATCCATCCGGCGTCCACCACGCACGCCCAGCTGAGCCCGGAGGAGCAACTGGCC
AGCGGCGTCACCCCCGGCCTGGTGCGGCTGGCCGTCGGCATCGAGGGCATCGACGACATCCTCGCCGACCTCGAGCAGGG
GTTCGCGGCTGCCAAGGCGATCTCCTGA

Upstream 100 bases:

>100_bases
GTGCCCCGGGTGATGACCAGGTTGAGTAGCCGTGACGGCTACGCGGCAAGCGCGGGTCCGTATCGACGGGCCCCCAGACA
CAGATGGAGGGCCGTTCCGT

Downstream 100 bases:

>100_bases
AGTTCGAAGAGACAGCAAGTGACGATTTTGAAGAGCCCCGCCGTGCCCGCCCTCGACCTGCCCGCCGAGGGTGAGACCGG
CGTGGTCGACATCGGCCCGC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSTPEDLIANWSFETKQVHAGQTPDAATNARALPIYQTTSYTFRDTDHAAALFGLAETGNIYTRIMNPTTDVVEQRIAAL
EGGVAALFLSSGQAAETFAILNIANAGDHIVSSPRLYGGTYNLFHYTLPKLGIDVSFVENPDDLDSWRAAVRPNTKAFFG
ETISNPQIDVLDIPNVAAVAHEAGVPLIVDNTIATPYLIQPLSHGADIVVHSATKYLGGHGNAIAGVIVDGGTFDWTNGR
FPGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGSAAAPFNAFLIAQGLETLSLRVERHVANAQKVAEYLAGHDDVL
SVNYAGLPSSPWYELGQRLAPKGTGAVLAFELAGGVDAGKAFVNALTLHSHVANIGDVRSLVIHPASTTHAQLSPEEQLA
SGVTPGLVRLAVGIEGIDDILADLEQGFAAAKAIS

Sequences:

>Translated_435_residues
MSTPEDLIANWSFETKQVHAGQTPDAATNARALPIYQTTSYTFRDTDHAAALFGLAETGNIYTRIMNPTTDVVEQRIAAL
EGGVAALFLSSGQAAETFAILNIANAGDHIVSSPRLYGGTYNLFHYTLPKLGIDVSFVENPDDLDSWRAAVRPNTKAFFG
ETISNPQIDVLDIPNVAAVAHEAGVPLIVDNTIATPYLIQPLSHGADIVVHSATKYLGGHGNAIAGVIVDGGTFDWTNGR
FPGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGSAAAPFNAFLIAQGLETLSLRVERHVANAQKVAEYLAGHDDVL
SVNYAGLPSSPWYELGQRLAPKGTGAVLAFELAGGVDAGKAFVNALTLHSHVANIGDVRSLVIHPASTTHAQLSPEEQLA
SGVTPGLVRLAVGIEGIDDILADLEQGFAAAKAIS
>Mature_434_residues
STPEDLIANWSFETKQVHAGQTPDAATNARALPIYQTTSYTFRDTDHAAALFGLAETGNIYTRIMNPTTDVVEQRIAALE
GGVAALFLSSGQAAETFAILNIANAGDHIVSSPRLYGGTYNLFHYTLPKLGIDVSFVENPDDLDSWRAAVRPNTKAFFGE
TISNPQIDVLDIPNVAAVAHEAGVPLIVDNTIATPYLIQPLSHGADIVVHSATKYLGGHGNAIAGVIVDGGTFDWTNGRF
PGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGSAAAPFNAFLIAQGLETLSLRVERHVANAQKVAEYLAGHDDVLS
VNYAGLPSSPWYELGQRLAPKGTGAVLAFELAGGVDAGKAFVNALTLHSHVANIGDVRSLVIHPASTTHAQLSPEEQLAS
GVTPGLVRLAVGIEGIDDILADLEQGFAAAKAIS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=403, Percent_Identity=32.0099255583127, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=29.2161520190024, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI25453487, Length=402, Percent_Identity=27.8606965174129, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1790375, Length=417, Percent_Identity=33.0935251798561, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1789383, Length=428, Percent_Identity=27.1028037383178, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=397, Percent_Identity=34.0050377833753, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=407, Percent_Identity=33.9066339066339, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17560056, Length=407, Percent_Identity=34.1523341523341, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=47.6851851851852, Blast_Score=342, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=36.214953271028, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321254, Length=448, Percent_Identity=26.1160714285714, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321170, Length=352, Percent_Identity=25.5681818181818, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=416, Percent_Identity=25.4807692307692, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=33.4916864608076, Blast_Score=215, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45850; Mature: 45719

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPEDLIANWSFETKQVHAGQTPDAATNARALPIYQTTSYTFRDTDHAAALFGLAETGN
CCCHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCCEEECCCCCHHHHEEECCCCC
IYTRIMNPTTDVVEQRIAALEGGVAALFLSSGQAAETFAILNIANAGDHIVSSPRLYGGT
EEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEECCE
YNLFHYTLPKLGIDVSFVENPDDLDSWRAAVRPNTKAFFGETISNPQIDVLDIPNVAAVA
EEEHEEEHHHHCCEEEECCCCCCHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCCHHHHH
HEAGVPLIVDNTIATPYLIQPLSHGADIVVHSATKYLGGHGNAIAGVIVDGGTFDWTNGR
HHCCCCEEECCCCCCCHHCCHHCCCCEEEEECHHHHHCCCCCEEEEEEEECCEEECCCCC
FPGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGSAAAPFNAFLIAQGLETLSLRVE
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHH
RHVANAQKVAEYLAGHDDVLSVNYAGLPSSPWYELGQRLAPKGTGAVLAFELAGGVDAGK
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHH
AFVNALTLHSHVANIGDVRSLVIHPASTTHAQLSPEEQLASGVTPGLVRLAVGIEGIDDI
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCHHHH
LADLEQGFAAAKAIS
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
STPEDLIANWSFETKQVHAGQTPDAATNARALPIYQTTSYTFRDTDHAAALFGLAETGN
CCHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCCEEECCCCCHHHHEEECCCCC
IYTRIMNPTTDVVEQRIAALEGGVAALFLSSGQAAETFAILNIANAGDHIVSSPRLYGGT
EEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEECCE
YNLFHYTLPKLGIDVSFVENPDDLDSWRAAVRPNTKAFFGETISNPQIDVLDIPNVAAVA
EEEHEEEHHHHCCEEEECCCCCCHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCCHHHHH
HEAGVPLIVDNTIATPYLIQPLSHGADIVVHSATKYLGGHGNAIAGVIVDGGTFDWTNGR
HHCCCCEEECCCCCCCHHCCHHCCCCEEEEECHHHHHCCCCCEEEEEEEECCEEECCCCC
FPGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGSAAAPFNAFLIAQGLETLSLRVE
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHH
RHVANAQKVAEYLAGHDDVLSVNYAGLPSSPWYELGQRLAPKGTGAVLAFELAGGVDAGK
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHH
AFVNALTLHSHVANIGDVRSLVIHPASTTHAQLSPEEQLASGVTPGLVRLAVGIEGIDDI
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCHHHH
LADLEQGFAAAKAIS
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]