Click here to switch to the map view.

The map label for this gene is isp [H]

Identifier: 119855352

GI number: 119855352

Start: 165117

End: 166499

Strand: Reverse

Name: isp [H]

Synonym: Mkms_5966

Alternate gene names: 119855352

Gene position: 166499-165117 (Counterclockwise)

Preceding gene: 119855353

Following gene: 119855351

Centisome position: 76.81

GC content: 67.25

Gene sequence:

>1383_bases
ATGCTTCGCCAAGTCGGTTCGATCGCGGCAGCGGTGGCGCTGCTGGTGGTATGCAGCCCGCCGCCACAGGCCGCCGCCTT
GACGATCCCCGTCGTCGATCCTGCCGCGCTGCCGCCCGATGGTCCTCCCTCGCCGAATCAGGAGATGCGTCAGAACGGCA
CATGCTCGTTCTACGGCGCCATGCCCGGCTTCGACCCGGCCGCTGTTCCGCCCAGCCAGAACATGCTCAACCTGCCTGAG
GCGTGGAAGACCTCACGCGGCAGGGGAGTGGCGGTAGCGGTCATCGACTCGGGAGTGACGCCCCAGCCGCGGCTGCCCAA
CCTCAACCCCGGCGGTGACTACATCGATCCCCCCGCCAACGGCCTGGTCGACTGTGACGGACACGGGACAGTGGTCGCCG
GCATCATCGGTGCTCAGCCTGGACCTGACGGCTTCTCTGGAGTCGCCCCCGAGGCGTCCATCGTGTCGATCCGGCAGAGT
TCAGCGCAATGGTCACCCAAGCAACCCCAAGGTGACGGTGACCCACAGCAGATCAAGACCGCCGGTGACGTCGCCACCCT
GGCTCGCGCAGTACGCCATGCCGCCGACATGCCCGCAGTAAAGGTCATCAACATCTCACTGACCGACTGCATCCCGGTGT
ACAAGCAGGTCGATCAGGCTGCCTTGGGCGCCGCGCTGCGCTACGCCGCGATCGATAAAGACATCGTGGTCATCGCGGCC
GCCGGCAACATCGGCGAAAACAACTGCGACAGTAACCCGTTGACAGATCCGAACCGCCCAGACGACCCCCGCAACTGGGC
TGGCGCCACGGTCATCTCCACCCCGTCGTACTGGCAGCCCTACGTCCTGTCGGTGGCGTCACTGACCCCTGAGGGCCAAC
CGTCCGGCTTCACCATGGCTGGTCCGTGGGTAGGAATCGCCGCGCCGGGGGAGCAGATCACCTCCCTGGGCAACGCCCCG
AATTCCGGGCTGGTCAACGGCCAGCCCTCCAACCAGGAGCCTCTGGTGCCGATCAATGGGACCAGCTTCGCGGCGGCCTA
CGTCTCCGGTGTCGCGGCGCTGGTGCGTTCAGCGCTGCCTGAGCTGACCGCGCGCCAGGTCGTCAACCGGCTCGTCGCGT
CAGCGCACAACGCGGCCCGTTCTCCGTCGAACCTTGTCGGCGCCGGCGTCATCGACCCGGTGGCGGCGCTGACCTGGGAC
ATCCCCGAGGGCGAAGAGGTGCCCGCCAATATGCCGGTCGTGCGGGTCGAACCGCCGGCGCCGCCGCCGCCGGACAACCG
CCTGCCACGGTTCATCGCATTCGGCGTGGGCATCGTCGCGATCGCCGCCGCCATCATCGCTGTGACCATGATTGGAATGC
GCCGTGGCAACGACAACCGTTAA

Upstream 100 bases:

>100_bases
ATCTCGACCTGTTCGAGAACACCCTGCTGATCACCATCGTGCCGGCCATGTTGTGGCTCGTCGGGCTGGTCAGTCTCATC
CGCAACCGGGGGTCGCTGTG

Downstream 100 bases:

>100_bases
CCGCCGCCGCTCCCGCATCGCGCCGGCAGTTCCGAGCTGGCGCAGGGTGACAGCGCTGGTGTCGCTGTTGCTGCTCGGCT
GGGCGGCGCTGCTGCCCGCC

Product: peptidase S8 and S53, subtilisin, kexin, sedolisin

Products: NA

Alternate protein names: ISP-1 [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLRQVGSIAAAVALLVVCSPPPQAAALTIPVVDPAALPPDGPPSPNQEMRQNGTCSFYGAMPGFDPAAVPPSQNMLNLPE
AWKTSRGRGVAVAVIDSGVTPQPRLPNLNPGGDYIDPPANGLVDCDGHGTVVAGIIGAQPGPDGFSGVAPEASIVSIRQS
SAQWSPKQPQGDGDPQQIKTAGDVATLARAVRHAADMPAVKVINISLTDCIPVYKQVDQAALGAALRYAAIDKDIVVIAA
AGNIGENNCDSNPLTDPNRPDDPRNWAGATVISTPSYWQPYVLSVASLTPEGQPSGFTMAGPWVGIAAPGEQITSLGNAP
NSGLVNGQPSNQEPLVPINGTSFAAAYVSGVAALVRSALPELTARQVVNRLVASAHNAARSPSNLVGAGVIDPVAALTWD
IPEGEEVPANMPVVRVEPPAPPPPDNRLPRFIAFGVGIVAIAAAIIAVTMIGMRRGNDNR

Sequences:

>Translated_460_residues
MLRQVGSIAAAVALLVVCSPPPQAAALTIPVVDPAALPPDGPPSPNQEMRQNGTCSFYGAMPGFDPAAVPPSQNMLNLPE
AWKTSRGRGVAVAVIDSGVTPQPRLPNLNPGGDYIDPPANGLVDCDGHGTVVAGIIGAQPGPDGFSGVAPEASIVSIRQS
SAQWSPKQPQGDGDPQQIKTAGDVATLARAVRHAADMPAVKVINISLTDCIPVYKQVDQAALGAALRYAAIDKDIVVIAA
AGNIGENNCDSNPLTDPNRPDDPRNWAGATVISTPSYWQPYVLSVASLTPEGQPSGFTMAGPWVGIAAPGEQITSLGNAP
NSGLVNGQPSNQEPLVPINGTSFAAAYVSGVAALVRSALPELTARQVVNRLVASAHNAARSPSNLVGAGVIDPVAALTWD
IPEGEEVPANMPVVRVEPPAPPPPDNRLPRFIAFGVGIVAIAAAIIAVTMIGMRRGNDNR
>Mature_460_residues
MLRQVGSIAAAVALLVVCSPPPQAAALTIPVVDPAALPPDGPPSPNQEMRQNGTCSFYGAMPGFDPAAVPPSQNMLNLPE
AWKTSRGRGVAVAVIDSGVTPQPRLPNLNPGGDYIDPPANGLVDCDGHGTVVAGIIGAQPGPDGFSGVAPEASIVSIRQS
SAQWSPKQPQGDGDPQQIKTAGDVATLARAVRHAADMPAVKVINISLTDCIPVYKQVDQAALGAALRYAAIDKDIVVIAA
AGNIGENNCDSNPLTDPNRPDDPRNWAGATVISTPSYWQPYVLSVASLTPEGQPSGFTMAGPWVGIAAPGEQITSLGNAP
NSGLVNGQPSNQEPLVPINGTSFAAAYVSGVAALVRSALPELTARQVVNRLVASAHNAARSPSNLVGAGVIDPVAALTWD
IPEGEEVPANMPVVRVEPPAPPPPDNRLPRFIAFGVGIVAIAAAIIAVTMIGMRRGNDNR

Specific function: Major intracellular protease produced by Bacillus subtilis [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 47204; Mature: 47204

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00137 SUBTILASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQVGSIAAAVALLVVCSPPPQAAALTIPVVDPAALPPDGPPSPNQEMRQNGTCSFYGA
CCCHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCEEEEEC
MPGFDPAAVPPSQNMLNLPEAWKTSRGRGVAVAVIDSGVTPQPRLPNLNPGGDYIDPPAN
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GLVDCDGHGTVVAGIIGAQPGPDGFSGVAPEASIVSIRQSSAQWSPKQPQGDGDPQQIKT
CEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHEEEEHHCCCCCCCCCCCCCCCHHHHHH
AGDVATLARAVRHAADMPAVKVINISLTDCIPVYKQVDQAALGAALRYAAIDKDIVVIAA
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
AGNIGENNCDSNPLTDPNRPDDPRNWAGATVISTPSYWQPYVLSVASLTPEGQPSGFTMA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEEC
GPWVGIAAPGEQITSLGNAPNSGLVNGQPSNQEPLVPINGTSFAAAYVSGVAALVRSALP
CCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
ELTARQVVNRLVASAHNAARSPSNLVGAGVIDPVAALTWDIPEGEEVPANMPVVRVEPPA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEECCCCCCCCCCCCCEEEECCCC
PPPPDNRLPRFIAFGVGIVAIAAAIIAVTMIGMRRGNDNR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLRQVGSIAAAVALLVVCSPPPQAAALTIPVVDPAALPPDGPPSPNQEMRQNGTCSFYGA
CCCHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCEEEEEC
MPGFDPAAVPPSQNMLNLPEAWKTSRGRGVAVAVIDSGVTPQPRLPNLNPGGDYIDPPAN
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GLVDCDGHGTVVAGIIGAQPGPDGFSGVAPEASIVSIRQSSAQWSPKQPQGDGDPQQIKT
CEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHEEEEHHCCCCCCCCCCCCCCCHHHHHH
AGDVATLARAVRHAADMPAVKVINISLTDCIPVYKQVDQAALGAALRYAAIDKDIVVIAA
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
AGNIGENNCDSNPLTDPNRPDDPRNWAGATVISTPSYWQPYVLSVASLTPEGQPSGFTMA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEEC
GPWVGIAAPGEQITSLGNAPNSGLVNGQPSNQEPLVPINGTSFAAAYVSGVAALVRSALP
CCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
ELTARQVVNRLVASAHNAARSPSNLVGAGVIDPVAALTWDIPEGEEVPANMPVVRVEPPA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEECCCCCCCCCCCCCEEEECCCC
PPPPDNRLPRFIAFGVGIVAIAAAIIAVTMIGMRRGNDNR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3087947; 9384377 [H]