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The map label for this gene is bphA [H]

Identifier: 119855241

GI number: 119855241

Start: 62426

End: 63769

Strand: Reverse

Name: bphA [H]

Synonym: Mkms_5855

Alternate gene names: 119855241

Gene position: 63769-62426 (Counterclockwise)

Preceding gene: 119855243

Following gene: 119855240

Centisome position: 29.42

GC content: 59.45

Gene sequence:

>1344_bases
GTGGATTCGATGGCGCCTGATGCGACGACAATGCGAACATTAGAGAATGCGCGCGGCTCCATCCTAAAGGGTCGCCTCCC
TGCGTCTCTCATCGCTAATGCAGCGCTTTACGAGCTTGAATTGAAGCGAGTATTTGGTAGGACCTGGCAGTTTCTCTGCC
ACGAAGACGAGATCCCCAATGCGGGTGACTATGTAGTGCGCTACATCGCTGATAACTCAATTATTGTCGCGCGGCAGCAG
GATATGACGATTCGGGCGATGTCGAACTCGTGTCGGCACCGCGGCACGCTGCTGTGCCGAACCGAGTCTGGGAATGAGTC
GGCGTTCCAGTGTCCGTACCACGGTTGGACCTATCGAAACAACGGTGATCTCATCGCGATACCTGCGCAGCAGGCAGTGT
ACGGTGCTGCGTTCGACAAGAGTCGGCTAGGGTTGCGCGCTCTGCCGATGCTGGACTCGTACGCGGGCCTTGTCTTCGGG
TGTGTGTCGGATGAGGCGCCGGGACTGGATGAGTACCTCGGGGACATGCGCTGGTATCTCGACTTGATGATGAAGAAGAG
CCCGGCCGGCCTTGAGGCGTGGGGTGCCCCGCAGCGTTGGGTGATTGACGCGAACTGGAAGACCGGCGCCGATAACTTTG
TTGGGGACGGCTATCACACGGTCATGACGCACCGTTCGATGTGCGAGCTGGGGTTGTTACCGCCCGATAATGTGGCCGTT
TCGCCGGCCCACGTCAGCCTATCGGGCGGGCACGGGGCGGGCGTTCTTGGCGCACCACCCGGCGTACCCGCACCGCCGTA
TATGGGCTATCCCGAGGAAGTCGTCGCCGGTCTCAGCGAGGGTTACGGCGATGACGTCCATGGCGAGTTGCTGAAACGGA
CGATGTTCATTCATGGCAATGTGTTCCCGAACTTGTCCTTCTTGAACGCCTTCATCGCCAAGGACGGGGAGTCTATGCCG
GTGCCCATTCTGACCTTGCGGCAATGGCGTCCCTTGGACGCAGCGCGTATGGAGGTGTGGTCGTGGTTCTTCGTGGAGCG
CAACGCGCCCGAGGAGTTCAAGCAGCAGTCGTTTGAGACTTATGTTCGGACGTTCGGGGTCGGGGGTGTCTTCGAGCAGG
ATGACGCCGAGATATTTCAGGCTATTACCAAGGGAACACGCGGCGAGTTGGCTGGTGGTGTGGAGCTGAACCTGGAGATG
GGACTGGACAATCTGGCTCCTGATCCAACGTGGCTGGGCCCGGGACGACCGTTGGCCAGTGGCTACGCCGAACAGAATCA
GCGCGAGTACTGGAAGCAGTACTTCGACTATCTGGCCACACCGAGAAGGGATGAGAACGTATGA

Upstream 100 bases:

>100_bases
CAAACAGTTATCATCCTATGTAAGCTTCCGTGTGGTGTAGCCCACATGAGTGCTCGCGTTCTACGGGCACAAATCACAAA
TCGATAGGAAGGTGCGCCCG

Downstream 100 bases:

>100_bases
CGACAACGGTGCCGCAGGATCCACCTCAGGCTGCGAGGGTTTCGCGCGACGTGCGCGAGGAAATCGAAGACTTCCTTTTC
GACGAGGCCGATCTGTTGGA

Product: ring hydroxylating dioxygenase, alpha subunit

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRVFGRTWQFLCHEDEIPNAGDYVVRYIADNSIIVARQQ
DMTIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRNNGDLIAIPAQQAVYGAAFDKSRLGLRALPMLDSYAGLVFG
CVSDEAPGLDEYLGDMRWYLDLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTHRSMCELGLLPPDNVAV
SPAHVSLSGGHGAGVLGAPPGVPAPPYMGYPEEVVAGLSEGYGDDVHGELLKRTMFIHGNVFPNLSFLNAFIAKDGESMP
VPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFETYVRTFGVGGVFEQDDAEIFQAITKGTRGELAGGVELNLEM
GLDNLAPDPTWLGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV

Sequences:

>Translated_447_residues
MDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRVFGRTWQFLCHEDEIPNAGDYVVRYIADNSIIVARQQ
DMTIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRNNGDLIAIPAQQAVYGAAFDKSRLGLRALPMLDSYAGLVFG
CVSDEAPGLDEYLGDMRWYLDLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTHRSMCELGLLPPDNVAV
SPAHVSLSGGHGAGVLGAPPGVPAPPYMGYPEEVVAGLSEGYGDDVHGELLKRTMFIHGNVFPNLSFLNAFIAKDGESMP
VPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFETYVRTFGVGGVFEQDDAEIFQAITKGTRGELAGGVELNLEM
GLDNLAPDPTWLGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV
>Mature_447_residues
MDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRVFGRTWQFLCHEDEIPNAGDYVVRYIADNSIIVARQQ
DMTIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRNNGDLIAIPAQQAVYGAAFDKSRLGLRALPMLDSYAGLVFG
CVSDEAPGLDEYLGDMRWYLDLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTHRSMCELGLLPPDNVAV
SPAHVSLSGGHGAGVLGAPPGVPAPPYMGYPEEVVAGLSEGYGDDVHGELLKRTMFIHGNVFPNLSFLNAFIAKDGESMP
VPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFETYVRTFGVGGVFEQDDAEIFQAITKGTRGELAGGVELNLEM
GLDNLAPDPTWLGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=397, Percent_Identity=35.5163727959698, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1788103, Length=207, Percent_Identity=30.9178743961353, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 49580; Mature: 49580

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRVFGRTWQFLCHEDEIPN
CCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AGDYVVRYIADNSIIVARQQDMTIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRN
CCCEEEEEEECCCEEEEECCCCEEEHHHHHHHCCCEEEEEECCCCCCEEECCCCCCEEEC
NGDLIAIPAQQAVYGAAFDKSRLGLRALPMLDSYAGLVFGCVSDEAPGLDEYLGDMRWYL
CCCEEEECCHHHHHHHHCCCCCCCCEECCHHHHHHHHEEEECCCCCCCHHHHHHHHHHHH
DLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTHRSMCELGLLPPDNVAV
HHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
SPAHVSLSGGHGAGVLGAPPGVPAPPYMGYPEEVVAGLSEGYGDDVHGELLKRTMFIHGN
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHEEECC
VFPNLSFLNAFIAKDGESMPVPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFET
CCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHEEEEEECCCHHHHHHHHHHH
YVRTFGVGGVFEQDDAEIFQAITKGTRGELAGGVELNLEMGLDNLAPDPTWLGPGRPLAS
HHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHC
GYAEQNQREYWKQYFDYLATPRRDENV
CHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRVFGRTWQFLCHEDEIPN
CCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AGDYVVRYIADNSIIVARQQDMTIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRN
CCCEEEEEEECCCEEEEECCCCEEEHHHHHHHCCCEEEEEECCCCCCEEECCCCCCEEEC
NGDLIAIPAQQAVYGAAFDKSRLGLRALPMLDSYAGLVFGCVSDEAPGLDEYLGDMRWYL
CCCEEEECCHHHHHHHHCCCCCCCCEECCHHHHHHHHEEEECCCCCCCHHHHHHHHHHHH
DLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTHRSMCELGLLPPDNVAV
HHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
SPAHVSLSGGHGAGVLGAPPGVPAPPYMGYPEEVVAGLSEGYGDDVHGELLKRTMFIHGN
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHEEECC
VFPNLSFLNAFIAKDGESMPVPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFET
CCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHEEEEEECCCHHHHHHHHHHH
YVRTFGVGGVFEQDDAEIFQAITKGTRGELAGGVELNLEMGLDNLAPDPTWLGPGRPLAS
HHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHC
GYAEQNQREYWKQYFDYLATPRRDENV
CHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1569021; 7592331 [H]