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The map label for this gene is gidB

Identifier: 119776786

GI number: 119776786

Start: 4303155

End: 4303775

Strand: Reverse

Name: gidB

Synonym: Sama_3654

Alternate gene names: 119776786

Gene position: 4303775-4303155 (Counterclockwise)

Preceding gene: 119776787

Following gene: 119776785

Centisome position: 99.95

GC content: 53.95

Gene sequence:

>621_bases
GTGTTAGCCGAAAAACTCAGTCAGGATCTCGCCAAAGCGGGCCTGCAGGTCGATGCCCAGCAGCAACAGCAATTACTTGC
CTTTGTGGCGCTGCTCGACAAGTGGAACAAGGCCTATAACCTGACGTCGGTGCGGGAACCGGCGCAAATGCTGACCCGGC
ACATTCTCGATAGCCTGGTGGTGTCACCTCATCTGGTGGGTTCGCGTTTTATCGATGTGGGCACAGGGCCTGGCTTGCCG
GGTATTCCGCTGGCTATCATCAATCCGGACAAAGAGTTTGTTTTGCTGGATAGTCTGGGCAAACGTATTCGTTTCCAAAA
ACAGGTTGCTGTGGAGCTTGGGCTGAAAAACATCAGCTCGGTGGAAAGCCGGGTCGAGCTGTATCAACCTGAGCAGGGAT
TTGATGGTGTGCTGAGTCGCGCGTTTGCCTCTGTGGGGGACATGCTCAGCTGGTGCCATCATTTACCAGCTGAAAACGGC
AGTTTTTATGCCCTCAAGGGCCAGCTTGGTGATGAAGAGATGGCCGGGATCCCCGAAGGCTTTAAGTTGATTGAAACCAT
CAGGCTCACAGTCCCCGGACTGGATGAGCAAAGGCATCTGCTGAAATTGGTCAAAGCGTGA

Upstream 100 bases:

>100_bases
CTCGCGCCACTCGCGTGCAAGGGAGGCCTCCGGGCTTCCCTTCACTTTTTCTGGGCCTCATAATAGCCCACCAAGACAGT
TGTACCGACGGAGATAGCCA

Downstream 100 bases:

>100_bases
CCCTCTGCCTTGCAATTGGCAGCAACATCAAGCAACTTGTCCAAGGCGTTATGTCTTGGTTGAACGACAGAATAACAACA
GGGTGAAATTGTGGGGAAAG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MLAEKLSQDLAKAGLQVDAQQQQQLLAFVALLDKWNKAYNLTSVREPAQMLTRHILDSLVVSPHLVGSRFIDVGTGPGLP
GIPLAIINPDKEFVLLDSLGKRIRFQKQVAVELGLKNISSVESRVELYQPEQGFDGVLSRAFASVGDMLSWCHHLPAENG
SFYALKGQLGDEEMAGIPEGFKLIETIRLTVPGLDEQRHLLKLVKA

Sequences:

>Translated_206_residues
MLAEKLSQDLAKAGLQVDAQQQQQLLAFVALLDKWNKAYNLTSVREPAQMLTRHILDSLVVSPHLVGSRFIDVGTGPGLP
GIPLAIINPDKEFVLLDSLGKRIRFQKQVAVELGLKNISSVESRVELYQPEQGFDGVLSRAFASVGDMLSWCHHLPAENG
SFYALKGQLGDEEMAGIPEGFKLIETIRLTVPGLDEQRHLLKLVKA
>Mature_206_residues
MLAEKLSQDLAKAGLQVDAQQQQQLLAFVALLDKWNKAYNLTSVREPAQMLTRHILDSLVVSPHLVGSRFIDVGTGPGLP
GIPLAIINPDKEFVLLDSLGKRIRFQKQVAVELGLKNISSVESRVELYQPEQGFDGVLSRAFASVGDMLSWCHHLPAENG
SFYALKGQLGDEEMAGIPEGFKLIETIRLTVPGLDEQRHLLKLVKA

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=205, Percent_Identity=59.5121951219512, Blast_Score=257, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_SHEAM (A1SBV0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_929526.1
- ProteinModelPortal:   A1SBV0
- SMR:   A1SBV0
- STRING:   A1SBV0
- GeneID:   4605901
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_3654
- NMPDR:   fig|326297.7.peg.3442
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- PhylomeDB:   A1SBV0
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 22679; Mature: 22679

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEKLSQDLAKAGLQVDAQQQQQLLAFVALLDKWNKAYNLTSVREPAQMLTRHILDSLV
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VSPHLVGSRFIDVGTGPGLPGIPLAIINPDKEFVLLDSLGKRIRFQKQVAVELGLKNISS
HCCHHHCCCEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VESRVELYQPEQGFDGVLSRAFASVGDMLSWCHHLPAENGSFYALKGQLGDEEMAGIPEG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCHH
FKLIETIRLTVPGLDEQRHLLKLVKA
HHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLAEKLSQDLAKAGLQVDAQQQQQLLAFVALLDKWNKAYNLTSVREPAQMLTRHILDSLV
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VSPHLVGSRFIDVGTGPGLPGIPLAIINPDKEFVLLDSLGKRIRFQKQVAVELGLKNISS
HCCHHHCCCEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VESRVELYQPEQGFDGVLSRAFASVGDMLSWCHHLPAENGSFYALKGQLGDEEMAGIPEG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCHH
FKLIETIRLTVPGLDEQRHLLKLVKA
HHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA