Click here to switch to the map view.

The map label for this gene is mscM [H]

Identifier: 119776764

GI number: 119776764

Start: 4275313

End: 4276494

Strand: Reverse

Name: mscM [H]

Synonym: Sama_3632

Alternate gene names: 119776764

Gene position: 4276494-4275313 (Counterclockwise)

Preceding gene: 119776765

Following gene: 119776759

Centisome position: 99.31

GC content: 51.27

Gene sequence:

>1182_bases
ATGATCGCCATGTTGGGTGTCTCTTTCGTCATCGCTGCAGTTGCTTATCTTATAGTTCGCCGTGGTGTGGTGAAGGCCAT
GAACCTGGTCATCCAGCGTTCCAGTGTGACTTGGGACGATGTGTTTATGAAGTACAAGGTACTGGAAAAGCTGTCCATGC
TGGTACCTGCCATGGTGCTCGATTTGTTGCTGCCCATCGTGCTGGCTGAGCACAAACTGGCTGCGAATCTGGCCGACCGT
CTTATTAATGTCGCCTTGGTAATATTGCTTATCAGAGCTATTTATTCGGCGTTGGACGCTGTGAATGAAATTGCGGAAAT
CCGTGATATCAGTCGCCGCTTACCGGTAAAAAGTGTTGTGCAGCTGATTAAGCTTTTCCTGTTCTTTGTTGGGGTGATTA
TCGGCATCTCGGTGCTGGCCGATCAGTCACCGGTCATCTTCCTTTCCGGTTTGGGTGTGGCGACTGGTTTGGTGATGTTG
GTGTTCAAGGACACCATCCTGGGCTTTGTGGCGGGTATACAGCTTGCCGCCAACCGTATGGTGAGCAAGGGTGACTGGAT
CCAGATGGACAAGTACGGTGCCGACGGTGCCGTGGAGGAAGTATCACTCACCACGGTAAAGGTGCGTAACTGGGACAATA
CCCTGACCATGATCCCGGCCTATGCTCTGGTCTCCGATGCCTTCCGTAACTGGCGTGGCATGTCTGAGTCGGGTGGCCGT
CGTATTAAGCGGGCGCTGAATATCGACATCAACAGCATACGTTTTCTGACTGAAGAAGACAGGGTACGACTGTCGCGCAT
CAATTACCTGAAAGACTACCTGCCCCGTAAGATAATTGAGGTGACGGAAGCCAATGCCGGCATTGCCGATATCGATGTAC
CTGTAAACGGCAGGCGTCTCACCAATGTGGGGACCTTTCGTGCTTATTTGACTGAATATCTGCGTCATCACGACAAGGTG
CACAAGGATATGACTTTGATGGTGCGTCAGCTTGCGCCCACCACTGAAGGTCTGCCGCTGGAGCTTTATATTTTTACCAA
CGACACCCGTTGGGTGGTTTACGAGGGGATCCAGGCAGACATTTTTGACCACATCTTTGCGATTCTGCCGGAATTTGGTT
TGACGGCATTCCAAAATCCCACCGGGGAAGATATTCGCAGTTTACGGCCGGTTAATTCTTGA

Upstream 100 bases:

>100_bases
TATTTGTCGGGATAAGACAGTGGATCAGGAATACAGAAACGAACTGGTGAATTGGCTGACTTCGATGGGGCTGCGGCCAG
AGGACTCGCAGGGGATCTCT

Downstream 100 bases:

>100_bases
TAGCCAGAAGGGCAGCGAGGCTGACAAAGACGCCGCCACTGGTTCTGTCGAACCAATGCAGACGATTGCTGGCACTGAGT
TTGGGTGCCAGCAAACTGGC

Product: putative small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MIAMLGVSFVIAAVAYLIVRRGVVKAMNLVIQRSSVTWDDVFMKYKVLEKLSMLVPAMVLDLLLPIVLAEHKLAANLADR
LINVALVILLIRAIYSALDAVNEIAEIRDISRRLPVKSVVQLIKLFLFFVGVIIGISVLADQSPVIFLSGLGVATGLVML
VFKDTILGFVAGIQLAANRMVSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDNTLTMIPAYALVSDAFRNWRGMSESGGR
RIKRALNIDINSIRFLTEEDRVRLSRINYLKDYLPRKIIEVTEANAGIADIDVPVNGRRLTNVGTFRAYLTEYLRHHDKV
HKDMTLMVRQLAPTTEGLPLELYIFTNDTRWVVYEGIQADIFDHIFAILPEFGLTAFQNPTGEDIRSLRPVNS

Sequences:

>Translated_393_residues
MIAMLGVSFVIAAVAYLIVRRGVVKAMNLVIQRSSVTWDDVFMKYKVLEKLSMLVPAMVLDLLLPIVLAEHKLAANLADR
LINVALVILLIRAIYSALDAVNEIAEIRDISRRLPVKSVVQLIKLFLFFVGVIIGISVLADQSPVIFLSGLGVATGLVML
VFKDTILGFVAGIQLAANRMVSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDNTLTMIPAYALVSDAFRNWRGMSESGGR
RIKRALNIDINSIRFLTEEDRVRLSRINYLKDYLPRKIIEVTEANAGIADIDVPVNGRRLTNVGTFRAYLTEYLRHHDKV
HKDMTLMVRQLAPTTEGLPLELYIFTNDTRWVVYEGIQADIFDHIFAILPEFGLTAFQNPTGEDIRSLRPVNS
>Mature_393_residues
MIAMLGVSFVIAAVAYLIVRRGVVKAMNLVIQRSSVTWDDVFMKYKVLEKLSMLVPAMVLDLLLPIVLAEHKLAANLADR
LINVALVILLIRAIYSALDAVNEIAEIRDISRRLPVKSVVQLIKLFLFFVGVIIGISVLADQSPVIFLSGLGVATGLVML
VFKDTILGFVAGIQLAANRMVSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDNTLTMIPAYALVSDAFRNWRGMSESGGR
RIKRALNIDINSIRFLTEEDRVRLSRINYLKDYLPRKIIEVTEANAGIADIDVPVNGRRLTNVGTFRAYLTEYLRHHDKV
HKDMTLMVRQLAPTTEGLPLELYIFTNDTRWVVYEGIQADIFDHIFAILPEFGLTAFQNPTGEDIRSLRPVNS

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=388, Percent_Identity=47.4226804123711, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 44018; Mature: 44018

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAMLGVSFVIAAVAYLIVRRGVVKAMNLVIQRSSVTWDDVFMKYKVLEKLSMLVPAMVL
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DLLLPIVLAEHKLAANLADRLINVALVILLIRAIYSALDAVNEIAEIRDISRRLPVKSVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
QLIKLFLFFVGVIIGISVLADQSPVIFLSGLGVATGLVMLVFKDTILGFVAGIQLAANRM
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDNTLTMIPAYALVSDAFRNWRGMSESGGR
CCCCCCEEEECCCCCCCHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
RIKRALNIDINSIRFLTEEDRVRLSRINYLKDYLPRKIIEVTEANAGIADIDVPVNGRRL
HHHHHHCCCHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEE
TNVGTFRAYLTEYLRHHDKVHKDMTLMVRQLAPTTEGLPLELYIFTNDTRWVVYEGIQAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCHHH
IFDHIFAILPEFGLTAFQNPTGEDIRSLRPVNS
HHHHHHHHHHHHCCHHCCCCCCHHHHHCCCCCC
>Mature Secondary Structure
MIAMLGVSFVIAAVAYLIVRRGVVKAMNLVIQRSSVTWDDVFMKYKVLEKLSMLVPAMVL
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DLLLPIVLAEHKLAANLADRLINVALVILLIRAIYSALDAVNEIAEIRDISRRLPVKSVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
QLIKLFLFFVGVIIGISVLADQSPVIFLSGLGVATGLVMLVFKDTILGFVAGIQLAANRM
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSKGDWIQMDKYGADGAVEEVSLTTVKVRNWDNTLTMIPAYALVSDAFRNWRGMSESGGR
CCCCCCEEEECCCCCCCHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
RIKRALNIDINSIRFLTEEDRVRLSRINYLKDYLPRKIIEVTEANAGIADIDVPVNGRRL
HHHHHHCCCHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEE
TNVGTFRAYLTEYLRHHDKVHKDMTLMVRQLAPTTEGLPLELYIFTNDTRWVVYEGIQAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCHHH
IFDHIFAILPEFGLTAFQNPTGEDIRSLRPVNS
HHHHHHHHHHHHCCHHCCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]