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The map label for this gene is rlmF

Identifier: 119776753

GI number: 119776753

Start: 4261104

End: 4262063

Strand: Reverse

Name: rlmF

Synonym: Sama_3621

Alternate gene names: 119776753

Gene position: 4262063-4261104 (Counterclockwise)

Preceding gene: 119776754

Following gene: 119776750

Centisome position: 98.98

GC content: 56.98

Gene sequence:

>960_bases
ATGGCGCCGTTTTTCTCTGCTATGACCTCAAAAAAACAATCCCAGGGGCTGCCTAAGGGGCCGCATCCTGACAATGCCCA
TCGCGATGGCTATGACTTTTCCGCGCTGGTGGCGAGTCATCCTCCGCTGAAACCCTTTGTGCGCGCCAACGCCTATGGCA
ATTTGTCCATCGACTTTGCCCTCCCTGAGGCGGTGAAAGCGCTCAACTGCGCCCTCCTGAAGCATCACTATGGCATCAGC
CGCTGGGATATTCCCAAGGGCTTTTTGTGCCCCCCAATCCCGGGCCGGGTAGATTATCTGCATCATCTTGAAGATCTGCT
CAGGCAAACACCGGGAACCGATGGATTACCCCTGCTGGATATAGGCACGGGAGCCAATGGTATTTATGCGCTGCTGGCGG
CGAGCCGATTCGGCCGGGCCGTGGTGGCAACGGATATTGCCAAGGCCTCGCTCACCAATGTGGCGACCATACTCAAAGCC
AATCCAGGGCTCGAAAAACGCATTAGTCTGCGTTTTCAAAGCAATCCCAGGCATATTCTTACCGGCGTAACCCAGACAAA
CGAGCAGTTCGCAGCGTGCGTCTGTAACCCGCCGTTTCATGCCAGTGCCGCCGAAGCGGCCTTGGGGACTAACCGTAAAC
TCGAAGGATTGGCCAAGAGCCGTGGTCAGCGCCACATCAGTGGGCCGGGTAAGCCGCAGACACTCAATTTTGGTGGTCAG
GATGCCGAGCTGTGGTGCGATGGCGGAGAGCGCAGCTTTCTGCTGCGCCTGATTGACGAAAGTGCCCGTCTGCCGGACTT
GTGCGTGTGGTTTACGACGCTGGTATCCAAAAGCGATAACCTGCGCCCCTGCAAGCGACGTTTGGAGCAGCGCGGCGCCA
GTGAAATCAAGGTGATAGAAATGCAGCAGGGGCAAAAAATCACCCGGATACTGGCCTGGCGCTTTGAGTCTGTTACCTGA

Upstream 100 bases:

>100_bases
GCCGTCAACCGACTCATCGAAGGTGAAACGGGCAAAGCACTGATAGTAAACCTGCTTTTTACCCGATACCTGTACGATTA
AAGGCCAAAGGCGGCGCCGG

Downstream 100 bases:

>100_bases
TTACTGTCCCTGGGACCAGAGCAGCTGCCCGGAGCCACTTAAGTCGTAACCGCCCAACATGGCCGCTACCTGGCGGGTGT
CGGGGGCGGTCAGCATGCCC

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MAPFFSAMTSKKQSQGLPKGPHPDNAHRDGYDFSALVASHPPLKPFVRANAYGNLSIDFALPEAVKALNCALLKHHYGIS
RWDIPKGFLCPPIPGRVDYLHHLEDLLRQTPGTDGLPLLDIGTGANGIYALLAASRFGRAVVATDIAKASLTNVATILKA
NPGLEKRISLRFQSNPRHILTGVTQTNEQFAACVCNPPFHASAAEAALGTNRKLEGLAKSRGQRHISGPGKPQTLNFGGQ
DAELWCDGGERSFLLRLIDESARLPDLCVWFTTLVSKSDNLRPCKRRLEQRGASEIKVIEMQQGQKITRILAWRFESVT

Sequences:

>Translated_319_residues
MAPFFSAMTSKKQSQGLPKGPHPDNAHRDGYDFSALVASHPPLKPFVRANAYGNLSIDFALPEAVKALNCALLKHHYGIS
RWDIPKGFLCPPIPGRVDYLHHLEDLLRQTPGTDGLPLLDIGTGANGIYALLAASRFGRAVVATDIAKASLTNVATILKA
NPGLEKRISLRFQSNPRHILTGVTQTNEQFAACVCNPPFHASAAEAALGTNRKLEGLAKSRGQRHISGPGKPQTLNFGGQ
DAELWCDGGERSFLLRLIDESARLPDLCVWFTTLVSKSDNLRPCKRRLEQRGASEIKVIEMQQGQKITRILAWRFESVT
>Mature_318_residues
APFFSAMTSKKQSQGLPKGPHPDNAHRDGYDFSALVASHPPLKPFVRANAYGNLSIDFALPEAVKALNCALLKHHYGISR
WDIPKGFLCPPIPGRVDYLHHLEDLLRQTPGTDGLPLLDIGTGANGIYALLAASRFGRAVVATDIAKASLTNVATILKAN
PGLEKRISLRFQSNPRHILTGVTQTNEQFAACVCNPPFHASAAEAALGTNRKLEGLAKSRGQRHISGPGKPQTLNFGGQD
AELWCDGGERSFLLRLIDESARLPDLCVWFTTLVSKSDNLRPCKRRLEQRGASEIKVIEMQQGQKITRILAWRFESVT

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=305, Percent_Identity=32.4590163934426, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI87081786, Length=298, Percent_Identity=44.9664429530201, Blast_Score=267, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI32564931, Length=305, Percent_Identity=31.8032786885246, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32564933, Length=305, Percent_Identity=29.1803278688525, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281363415, Length=301, Percent_Identity=26.578073089701, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922302, Length=301, Percent_Identity=26.578073089701, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_SHEAM (A1SBR7)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_929493.1
- ProteinModelPortal:   A1SBR7
- SMR:   A1SBR7
- STRING:   A1SBR7
- GeneID:   4605868
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_3621
- NMPDR:   fig|326297.7.peg.3411
- eggNOG:   COG3129
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- PhylomeDB:   A1SBR7
- ProtClustDB:   PRK11727
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 34906; Mature: 34775

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPFFSAMTSKKQSQGLPKGPHPDNAHRDGYDFSALVASHPPLKPFVRANAYGNLSIDFA
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCCCCCCEEEEEE
LPEAVKALNCALLKHHYGISRWDIPKGFLCPPIPGRVDYLHHLEDLLRQTPGTDGLPLLD
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
IGTGANGIYALLAASRFGRAVVATDIAKASLTNVATILKANPGLEKRISLRFQSNPRHIL
ECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
TGVTQTNEQFAACVCNPPFHASAAEAALGTNRKLEGLAKSRGQRHISGPGKPQTLNFGGQ
ECCCCCCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCEECCCCC
DAELWCDGGERSFLLRLIDESARLPDLCVWFTTLVSKSDNLRPCKRRLEQRGASEIKVIE
CCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEE
MQQGQKITRILAWRFESVT
ECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
APFFSAMTSKKQSQGLPKGPHPDNAHRDGYDFSALVASHPPLKPFVRANAYGNLSIDFA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCCCCCCEEEEEE
LPEAVKALNCALLKHHYGISRWDIPKGFLCPPIPGRVDYLHHLEDLLRQTPGTDGLPLLD
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
IGTGANGIYALLAASRFGRAVVATDIAKASLTNVATILKANPGLEKRISLRFQSNPRHIL
ECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
TGVTQTNEQFAACVCNPPFHASAAEAALGTNRKLEGLAKSRGQRHISGPGKPQTLNFGGQ
ECCCCCCCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCEECCCCC
DAELWCDGGERSFLLRLIDESARLPDLCVWFTTLVSKSDNLRPCKRRLEQRGASEIKVIE
CCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEE
MQQGQKITRILAWRFESVT
ECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA