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The map label for this gene is fdhB1 [H]

Identifier: 119776744

GI number: 119776744

Start: 4251705

End: 4252301

Strand: Reverse

Name: fdhB1 [H]

Synonym: Sama_3612

Alternate gene names: 119776744

Gene position: 4252301-4251705 (Counterclockwise)

Preceding gene: 119776745

Following gene: 119776743

Centisome position: 98.75

GC content: 57.62

Gene sequence:

>597_bases
ATGGCAGTCATGAAATTTCTGTGTGACACCAAGCGCTGCATCGAGTGTAACGGTTGTGTCACCGCTTGTAAGAACGAAAA
CGACTCCGCTCTCGAGTGGGGTATTCAGCGTCGCCGTGTAGTCACCATCCGTGATGGTGAACCCGGCGAGGCCTCAATCT
CGGTTGCCTGTATGCACTGTACCGACGCGCCCTGTATGGCGGTGTGTCCGGCGAACTGCTTCTACAAAACCGACGACGGC
ATAGTGCTGCACAACAAAGATACCTGTATCGGTTGTGGCTACTGCTTCTACGCCTGTCCGTTCGGTGCGCCGCAGTTCCC
GAAAAAGACCGCCTTTGGCAGTCGCGGCAAGATGGACAAGTGCACCTTCTGCGCCGGTGGCCCTGAGGAGAACTTCTCCG
ACGCCGAACGCCAGAAGTACGGTGCCAACCGTATCGCCGAAGGCAAACTGCCTATGTGCGCCGAGCTGTGTGCCACCAAG
GCGCTGCTCGCCGGTGATGCAGATGTCGTATCCAGCATCTACCGTGAGCGTATGGCTGCCCGCGGCAATCCCAACGTGAT
CTGGGGCTACAACCCTCAGACAGGTGAGATTAACTGA

Upstream 100 bases:

>100_bases
GACCTATGGTTATGACCCTGTGACCCAGATGCAGGAAACCAAGTCCAGTCTGTGCCAAATCACTAAAGCCTGACCGTAAC
TTGATGAGGAGAAAGCCATT

Downstream 100 bases:

>100_bases
GGCCAAGCTGTGGCAACAGGGCGCCTTAAATGGGCTCTGTTGCCGCTATAGGAGTGAAAATGTTATCCAAAACGTTTAAC
AAGTCACTGCAGCAGCTCTG

Product: formate dehydrogenase, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MAVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHCTDAPCMAVCPANCFYKTDDG
IVLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDKCTFCAGGPEENFSDAERQKYGANRIAEGKLPMCAELCATK
ALLAGDADVVSSIYRERMAARGNPNVIWGYNPQTGEIN

Sequences:

>Translated_198_residues
MAVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHCTDAPCMAVCPANCFYKTDDG
IVLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDKCTFCAGGPEENFSDAERQKYGANRIAEGKLPMCAELCATK
ALLAGDADVVSSIYRERMAARGNPNVIWGYNPQTGEIN
>Mature_197_residues
AVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHCTDAPCMAVCPANCFYKTDDGI
VLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDKCTFCAGGPEENFSDAERQKYGANRIAEGKLPMCAELCATKA
LLAGDADVVSSIYRERMAARGNPNVIWGYNPQTGEIN

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=190, Percent_Identity=32.6315789473684, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787872, Length=171, Percent_Identity=34.5029239766082, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1789370, Length=213, Percent_Identity=29.1079812206573, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI226510944, Length=164, Percent_Identity=32.9268292682927, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI2367345, Length=184, Percent_Identity=32.0652173913043, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1787749, Length=209, Percent_Identity=28.7081339712919, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790326, Length=192, Percent_Identity=28.125, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788811, Length=126, Percent_Identity=32.5396825396825, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI87082114, Length=189, Percent_Identity=31.2169312169312, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI87082180, Length=185, Percent_Identity=29.7297297297297, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789079, Length=101, Percent_Identity=35.6435643564356, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 21533; Mature: 21402

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
12.6 %Cys+Met (Translated Protein)
9.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHC
CHHHHHHHCCCCEEECCCCEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEC
TDAPCMAVCPANCFYKTDDGIVLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCCEEECCHHEEECCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEENFSDAERQKYGANRIAEGKLPMCAELCATKALLAGDADVVSSIYRERMAA
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGNPNVIWGYNPQTGEIN
CCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
AVMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTIRDGEPGEASISVACMHC
HHHHHHHCCCCEEECCCCEEECCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEC
TDAPCMAVCPANCFYKTDDGIVLHNKDTCIGCGYCFYACPFGAPQFPKKTAFGSRGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCCEEECCHHEEECCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEENFSDAERQKYGANRIAEGKLPMCAELCATKALLAGDADVVSSIYRERMAA
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGNPNVIWGYNPQTGEIN
CCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]