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The map label for this gene is bioH [H]

Identifier: 119776637

GI number: 119776637

Start: 4138394

End: 4139176

Strand: Direct

Name: bioH [H]

Synonym: Sama_3505

Alternate gene names: 119776637

Gene position: 4138394-4139176 (Clockwise)

Preceding gene: 119776635

Following gene: 119776638

Centisome position: 96.1

GC content: 58.49

Gene sequence:

>783_bases
GTGACACAAGGTTCACAGGCACATATCCATATCGACACCCATGGCACAGGCAAAAACCTGGTGATTCTCCACGGCTGGGG
CATGAACGCGGCTGTGTTCACGCCCCTTATGGGTGCCTTTCCCGAGTATAGGCTGCACTGCCCCGACCTGCCGGGATTCG
GCCATAGCACTATGTCTGGTGATACCCTGGATGATTGGGTCGACACCCTGATGTTGGCGCTGCCCGAGCAGATGGTGCTT
GCGGGTTGGTCACTTGGTGGCTTGGTGGCGACCAGAGCTGCACTGCGCTACCCCCAGCGGGTTGAGGCGCTGATAACCAT
TGCCTCATCCCCTTGTTTTATGGCGCGGGAGGAAGAAAACTGGCCCGGCATTCCACCCCAGGTATTGTCGCAATTTGGCG
CAGAACTGGGCAAAGATCTGCCCAAAACCATAGAGCGTTTTCTCGCTATCCAGGCCATGGGCAGCGAAACAGCGAGGGAA
GACATCAAGCGCCTGCGGGATTTGGTGCTGTCACGCCCCCTGCCCCAGCCAGCGGCGTTGGATCAGGGGCTGAACATGTT
AAAAGACGTGGATTTACGCCCCGAGCTGGCAAACCTGAGCCAACCCTGGTTGAGAGTGTGGGGACGCCTCGATGGGCTGG
TTCCCAAGCGGGTCATGCCGGCCATGCCAAGCCCTGCCGGTACCTGCGAGGACCTGATGCTCGCCAAAGCATCCCATGCC
CCTTTCTTTTCTCACCCCACTGAATTTGTGACCGGGGTGAAAGACTGGCTCTTCAGGCTTTAA

Upstream 100 bases:

>100_bases
CGGTTGGGTAAACTCTGCGCCAAGGTGGTAACGACATACGTCCATGCTCGGCGCCACACGGATACTTCATCCATGGCATC
ATCCCTGACTAGAGGCACAA

Downstream 100 bases:

>100_bases
TCTGCTCAATCGAGAGGTTATTATTTAATCAGCCTGTTTTTGGAGTTCGTCATGCTGGTTGTCACCAACTACCCTCAAGT
GCCATTAGCGACCAGTAATG

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTQGSQAHIHIDTHGTGKNLVILHGWGMNAAVFTPLMGAFPEYRLHCPDLPGFGHSTMSGDTLDDWVDTLMLALPEQMVL
AGWSLGGLVATRAALRYPQRVEALITIASSPCFMAREEENWPGIPPQVLSQFGAELGKDLPKTIERFLAIQAMGSETARE
DIKRLRDLVLSRPLPQPAALDQGLNMLKDVDLRPELANLSQPWLRVWGRLDGLVPKRVMPAMPSPAGTCEDLMLAKASHA
PFFSHPTEFVTGVKDWLFRL

Sequences:

>Translated_260_residues
MTQGSQAHIHIDTHGTGKNLVILHGWGMNAAVFTPLMGAFPEYRLHCPDLPGFGHSTMSGDTLDDWVDTLMLALPEQMVL
AGWSLGGLVATRAALRYPQRVEALITIASSPCFMAREEENWPGIPPQVLSQFGAELGKDLPKTIERFLAIQAMGSETARE
DIKRLRDLVLSRPLPQPAALDQGLNMLKDVDLRPELANLSQPWLRVWGRLDGLVPKRVMPAMPSPAGTCEDLMLAKASHA
PFFSHPTEFVTGVKDWLFRL
>Mature_259_residues
TQGSQAHIHIDTHGTGKNLVILHGWGMNAAVFTPLMGAFPEYRLHCPDLPGFGHSTMSGDTLDDWVDTLMLALPEQMVLA
GWSLGGLVATRAALRYPQRVEALITIASSPCFMAREEENWPGIPPQVLSQFGAELGKDLPKTIERFLAIQAMGSETARED
IKRLRDLVLSRPLPQPAALDQGLNMLKDVDLRPELANLSQPWLRVWGRLDGLVPKRVMPAMPSPAGTCEDLMLAKASHAP
FFSHPTEFVTGVKDWLFRL

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=240, Percent_Identity=49.1666666666667, Blast_Score=225, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28681; Mature: 28549

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQGSQAHIHIDTHGTGKNLVILHGWGMNAAVFTPLMGAFPEYRLHCPDLPGFGHSTMSG
CCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
DTLDDWVDTLMLALPEQMVLAGWSLGGLVATRAALRYPQRVEALITIASSPCFMAREEEN
CCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCEECCCCCC
WPGIPPQVLSQFGAELGKDLPKTIERFLAIQAMGSETAREDIKRLRDLVLSRPLPQPAAL
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
DQGLNMLKDVDLRPELANLSQPWLRVWGRLDGLVPKRVMPAMPSPAGTCEDLMLAKASHA
HHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHCCC
PFFSHPTEFVTGVKDWLFRL
CCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQGSQAHIHIDTHGTGKNLVILHGWGMNAAVFTPLMGAFPEYRLHCPDLPGFGHSTMSG
CCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
DTLDDWVDTLMLALPEQMVLAGWSLGGLVATRAALRYPQRVEALITIASSPCFMAREEEN
CCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCEECCCCCC
WPGIPPQVLSQFGAELGKDLPKTIERFLAIQAMGSETAREDIKRLRDLVLSRPLPQPAAL
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
DQGLNMLKDVDLRPELANLSQPWLRVWGRLDGLVPKRVMPAMPSPAGTCEDLMLAKASHA
HHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHCCC
PFFSHPTEFVTGVKDWLFRL
CCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA