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The map label for this gene is menE [H]

Identifier: 119776595

GI number: 119776595

Start: 4092847

End: 4094280

Strand: Direct

Name: menE [H]

Synonym: Sama_3463

Alternate gene names: 119776595

Gene position: 4092847-4094280 (Clockwise)

Preceding gene: 119776594

Following gene: 119776601

Centisome position: 95.05

GC content: 58.44

Gene sequence:

>1434_bases
GTGAGCCCTCAGCGTTCAGTCAGGCCTCAACTATCCATCTCGCCCCTGCAGCAAGCATCGGCCACCTGGCCCGACGCCCA
TGCACTGATAACGCCGGAATTCACCTTGTCTTTCGAGGTGCTGGGCAAGCTGGTGCGTGAGGTTGCCAGACAGCTTAAGA
CACAAGATATACAGCGCCTCGGGGTGATTGGGCCCAACAGTGTTGAGCAAATTGTCCTCTACTGGGCCTGCGCCGAAGCA
GGTGCCCTTTTTTGCCCCCTGTCATGGCGCTTTCCGGTTTCTCAGCTGGAGATTCTGATACGGCGTTTTGCCCTCGATGC
ACTGCATGCCCCGACGCTTGAAAGCCTTTGCCCTGCGTTACCCCGTATTCATGTTGCCGACATGATTGGCGGAATAGGGG
CAAGCTCCCTGACGACTCCGGCCAGCGCAGTATTGGACCTGTCGCAGCCGGTTAATCTGGTGCTGAGTTCAGGCTCCACG
GGCGAGCCTAAAGCCGCAGTACATGCTCTGGCCAATCATATTGCCAGCGCTGAGGGTGCCCGATGCCTTATCCCGCTGTC
ACCGGGCGATCGCTGGCTCCTGTCATTGCCGCTGTTTCATATCGGCGGTATCGCCATTCTCAATCGATGTGCCCTCGCCG
GGGCTGCGGTGGTGTTGCCACAGCAGCAAGCATCGACAAGACCACTGTCGATGGCGCAGGCCATGTCAGCACTGTCACCC
ACCCATGTCTCTTTGGTGGCGGCGCAGCTCACTGCGCTGCTTGAGGATTCGCCTGACTCACTTAGCTCAGTTAAGGCGTT
GCTGCTGGGGGGCAGCGCCATAGGTGGCGCGTTGCTTTCACGACTTGAAGCACTGGGGATACACGCTTTTACCAGCTACG
GCATGACAGAAATGAGCTCGCAAATCACCACAGGCCCCGCCAATAGCGAGGGCGCAAGCGGCACCCTGTTGCCGGGACGG
GAGCTCAGAATCCATCAGGGGGAAATTCAGGTACGAGGCGAGACTCTGTTCCTTGGGTATCTGGAGGATAACGGTCTGAG
ATTACCGCTCACCGATGACGGTTGGTTTGCGACCAAAGACTGCGGCCATCTGGACGAAGCCGGCAGACTTTTTGTGGCGG
GGCGGCTCGACAACATGTTTATCTGCGGCGGAGAAAACCTGCACCCGGAAGAAATTGAAGCGGCGCTGTGCCAACATCCT
CTGGTGCTGGAGGCCATCGTTTATCCCAAAGCTGATAACACTTTTGGATTCTTGCCCCATGCGCTGCTGCGCTGCCGCAA
TACCATGCCCTCCCAAGGGGAATTGGATGACTTCCTTGCAAGCCGTATCGCCCGCTTTAAACGGCCTCGCAGTTATCAGG
CATGGCCACAGATGGCAAGCAGCGGCCTGAAAACCAACCGCAAGGCGGTGGTGGCCGCAGTGCTGCGCCAGTGA

Upstream 100 bases:

>100_bases
CCTTTGAGGCCGACTGCATCGTCTCACAAGGCCAAAAGCGTTGCCTCACCCCAGACGCCCCGGGGCCTGTGCTTGGCTGT
GAACTCGGAGCCTCGCTGCT

Downstream 100 bases:

>100_bases
ATCACAGTGAATGCCAAGGCGCCAAAACCTGTTAATGCCTGACGGATAACCACAAGTAAAAATCCACCTGCATAGCAGGT
GGCTTTGACACGGCCCCCTA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSPQRSVRPQLSISPLQQASATWPDAHALITPEFTLSFEVLGKLVREVARQLKTQDIQRLGVIGPNSVEQIVLYWACAEA
GALFCPLSWRFPVSQLEILIRRFALDALHAPTLESLCPALPRIHVADMIGGIGASSLTTPASAVLDLSQPVNLVLSSGST
GEPKAAVHALANHIASAEGARCLIPLSPGDRWLLSLPLFHIGGIAILNRCALAGAAVVLPQQQASTRPLSMAQAMSALSP
THVSLVAAQLTALLEDSPDSLSSVKALLLGGSAIGGALLSRLEALGIHAFTSYGMTEMSSQITTGPANSEGASGTLLPGR
ELRIHQGEIQVRGETLFLGYLEDNGLRLPLTDDGWFATKDCGHLDEAGRLFVAGRLDNMFICGGENLHPEEIEAALCQHP
LVLEAIVYPKADNTFGFLPHALLRCRNTMPSQGELDDFLASRIARFKRPRSYQAWPQMASSGLKTNRKAVVAAVLRQ

Sequences:

>Translated_477_residues
MSPQRSVRPQLSISPLQQASATWPDAHALITPEFTLSFEVLGKLVREVARQLKTQDIQRLGVIGPNSVEQIVLYWACAEA
GALFCPLSWRFPVSQLEILIRRFALDALHAPTLESLCPALPRIHVADMIGGIGASSLTTPASAVLDLSQPVNLVLSSGST
GEPKAAVHALANHIASAEGARCLIPLSPGDRWLLSLPLFHIGGIAILNRCALAGAAVVLPQQQASTRPLSMAQAMSALSP
THVSLVAAQLTALLEDSPDSLSSVKALLLGGSAIGGALLSRLEALGIHAFTSYGMTEMSSQITTGPANSEGASGTLLPGR
ELRIHQGEIQVRGETLFLGYLEDNGLRLPLTDDGWFATKDCGHLDEAGRLFVAGRLDNMFICGGENLHPEEIEAALCQHP
LVLEAIVYPKADNTFGFLPHALLRCRNTMPSQGELDDFLASRIARFKRPRSYQAWPQMASSGLKTNRKAVVAAVLRQ
>Mature_476_residues
SPQRSVRPQLSISPLQQASATWPDAHALITPEFTLSFEVLGKLVREVARQLKTQDIQRLGVIGPNSVEQIVLYWACAEAG
ALFCPLSWRFPVSQLEILIRRFALDALHAPTLESLCPALPRIHVADMIGGIGASSLTTPASAVLDLSQPVNLVLSSGSTG
EPKAAVHALANHIASAEGARCLIPLSPGDRWLLSLPLFHIGGIAILNRCALAGAAVVLPQQQASTRPLSMAQAMSALSPT
HVSLVAAQLTALLEDSPDSLSSVKALLLGGSAIGGALLSRLEALGIHAFTSYGMTEMSSQITTGPANSEGASGTLLPGRE
LRIHQGEIQVRGETLFLGYLEDNGLRLPLTDDGWFATKDCGHLDEAGRLFVAGRLDNMFICGGENLHPEEIEAALCQHPL
VLEAIVYPKADNTFGFLPHALLRCRNTMPSQGELDDFLASRIARFKRPRSYQAWPQMASSGLKTNRKAVVAAVLRQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=515, Percent_Identity=24.2718446601942, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI27477105, Length=136, Percent_Identity=35.2941176470588, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788595, Length=377, Percent_Identity=36.8700265251989, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=25.4473161033797, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788107, Length=279, Percent_Identity=26.1648745519713, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1786801, Length=356, Percent_Identity=25.8426966292135, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32563687, Length=409, Percent_Identity=25.9168704156479, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=465, Percent_Identity=24.0860215053763, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=484, Percent_Identity=24.3801652892562, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=481, Percent_Identity=26.6112266112266, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=375, Percent_Identity=24.8, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17538037, Length=369, Percent_Identity=23.8482384823848, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=328, Percent_Identity=25.9146341463415, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=349, Percent_Identity=24.0687679083095, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=348, Percent_Identity=29.0229885057471, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI18859661, Length=357, Percent_Identity=25.4901960784314, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356947, Length=322, Percent_Identity=26.3975155279503, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648260, Length=404, Percent_Identity=23.2673267326733, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355181, Length=344, Percent_Identity=25.5813953488372, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921316, Length=142, Percent_Identity=33.0985915492958, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50989; Mature: 50858

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPQRSVRPQLSISPLQQASATWPDAHALITPEFTLSFEVLGKLVREVARQLKTQDIQRL
CCCCCCCCCCCCCCHHHHCCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
GVIGPNSVEQIVLYWACAEAGALFCPLSWRFPVSQLEILIRRFALDALHAPTLESLCPAL
CCCCCCCHHHHHHHEEHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHC
PRIHVADMIGGIGASSLTTPASAVLDLSQPVNLVLSSGSTGEPKAAVHALANHIASAEGA
CCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
RCLIPLSPGDRWLLSLPLFHIGGIAILNRCALAGAAVVLPQQQASTRPLSMAQAMSALSP
EEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCEEEECCHHCCCCCHHHHHHHHHCCC
THVSLVAAQLTALLEDSPDSLSSVKALLLGGSAIGGALLSRLEALGIHAFTSYGMTEMSS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QITTGPANSEGASGTLLPGRELRIHQGEIQVRGETLFLGYLEDNGLRLPLTDDGWFATKD
HCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECEEEEEEEEECCCEEEEECCCCCEECCC
CGHLDEAGRLFVAGRLDNMFICGGENLHPEEIEAALCQHPLVLEAIVYPKADNTFGFLPH
CCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHH
ALLRCRNTMPSQGELDDFLASRIARFKRPRSYQAWPQMASSGLKTNRKAVVAAVLRQ
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SPQRSVRPQLSISPLQQASATWPDAHALITPEFTLSFEVLGKLVREVARQLKTQDIQRL
CCCCCCCCCCCCCHHHHCCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
GVIGPNSVEQIVLYWACAEAGALFCPLSWRFPVSQLEILIRRFALDALHAPTLESLCPAL
CCCCCCCHHHHHHHEEHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHC
PRIHVADMIGGIGASSLTTPASAVLDLSQPVNLVLSSGSTGEPKAAVHALANHIASAEGA
CCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
RCLIPLSPGDRWLLSLPLFHIGGIAILNRCALAGAAVVLPQQQASTRPLSMAQAMSALSP
EEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCEEEECCHHCCCCCHHHHHHHHHCCC
THVSLVAAQLTALLEDSPDSLSSVKALLLGGSAIGGALLSRLEALGIHAFTSYGMTEMSS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QITTGPANSEGASGTLLPGRELRIHQGEIQVRGETLFLGYLEDNGLRLPLTDDGWFATKD
HCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECEEEEEEEEECCCEEEEECCCCCEECCC
CGHLDEAGRLFVAGRLDNMFICGGENLHPEEIEAALCQHPLVLEAIVYPKADNTFGFLPH
CCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHH
ALLRCRNTMPSQGELDDFLASRIARFKRPRSYQAWPQMASSGLKTNRKAVVAAVLRQ
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]