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The map label for this gene is mdtL [H]

Identifier: 119776580

GI number: 119776580

Start: 4078575

End: 4079777

Strand: Reverse

Name: mdtL [H]

Synonym: Sama_3448

Alternate gene names: 119776580

Gene position: 4079777-4078575 (Counterclockwise)

Preceding gene: 119776583

Following gene: 119776579

Centisome position: 94.74

GC content: 56.19

Gene sequence:

>1203_bases
ATGCAACGAAATCTGCTGCCTATTTTGATGTCTCTGGTGCTGTTAAGCCCGCTGGCCATCGACATCTATCTGCCCTCAAT
GCCTGCCATGGCGCTGGATTTCAGCGTGGACGAGCGGCAGGTGCAGTCGACTCTGGTGCTGTTTTTGTTTGCCATGGGCG
TTGGCCAGGTATTGATAGGCCCATTGGCCGACCGTTACGGTCGTCGTCCGGTGGCGCTCTTCGGCACAGTGCTCTATGTG
ATCAGCAGTCTGCTGGCCATGGCCGCTCTGGAGTTTGAATTGCTGCAACTGGCCCGCCTGCTACAGGGGCTTGCAGCCTG
CTCCACTTCCATCGTTGCCTTCAGCGCAGTGCGGGACAGCTACAGTTCTGCCGACAGCGCCCGTATTTACAGTTACCTCA
ATGGTGCCATCTGCGTGATCCCGGCGCTGGCACCTACCCTCGGTGGCTTATTGGCTATTCAGTTTGGCTGGCGTTCGACC
TTTGCCTTTATGGCCTTTTATGGGTTGGTGATGTTGTTGGTGGTGGGCTACCGCTTGCCGGAAACCCGTCCAGCCCATAC
TGAATCCGCAGGTCCTCTGTACCGCTGGGGACGTTATGCCCCTGTGCTCAAAAACAGCCATTTCATGTTTTATGCCGTGA
CCTGCATGGCCGCCATGGCCGCGATCCTTTGCTACGTTTCTTATTCGTCCATTTGGCTGATTGGCAAACTGGGTGTGTCT
GAACTGGCGTTTTCGGGGCTCTTCGGTCTGAATGCCGTGGTGAACGTGGTGGCCTGTTTTGCAGCGCCCGTGGTGATCCG
TAAGTTAGGGAACCGTCCAACCGTGTTGGTGGCGCTGGGCTTAATGATATTGGCATCGGTGCTGGAGCTGGTATTGCAAC
TGCCTGCATGGGCATCACCCATGGCTGCTTCTTTTGCTTTTATGTTGCCTATGATGCTGCTGTGTGTGGGTTTTGCATTC
TTGCTTGGTCCCGCCACCAGTATGGCGCTGGCACCCTTCGGTGAGCGTGCCGGTACCGCCACGGCGCTGCTGGGCTTTAT
TCAAATGAGTGGGGCGTCTGTGATTACCGCACTGGTTCAACTCACACCGATGACCGCACCTTATGCCATTGGTTACACCA
TGGGCGGCCTGGCCTTGGTGCTGCTGGCCATCATGTTGATGCCACGGTTTGGCCATTGGCATCAGGAACCAAGTACTCAT
TGA

Upstream 100 bases:

>100_bases
CAAAGGGGAATTTTTAAAAGTAATTTATACCATTTCTGTTCATACCATGGCAGGAGTAAACTTCATCTATAATTTTTCTT
TACCAACCTGTGACTTGTCC

Downstream 100 bases:

>100_bases
TACCCAGTTATCACCCTTTAGCCTGTCCGGGTTTCCCCGTGACGGGCTTTTTTTTATTATGGGCAAAATGCGTTGTAATG
GGTGTTAATGGTGTCCCGGG

Product: drug resistance transporter, Bcr/CflA family protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MQRNLLPILMSLVLLSPLAIDIYLPSMPAMALDFSVDERQVQSTLVLFLFAMGVGQVLIGPLADRYGRRPVALFGTVLYV
ISSLLAMAALEFELLQLARLLQGLAACSTSIVAFSAVRDSYSSADSARIYSYLNGAICVIPALAPTLGGLLAIQFGWRST
FAFMAFYGLVMLLVVGYRLPETRPAHTESAGPLYRWGRYAPVLKNSHFMFYAVTCMAAMAAILCYVSYSSIWLIGKLGVS
ELAFSGLFGLNAVVNVVACFAAPVVIRKLGNRPTVLVALGLMILASVLELVLQLPAWASPMAASFAFMLPMMLLCVGFAF
LLGPATSMALAPFGERAGTATALLGFIQMSGASVITALVQLTPMTAPYAIGYTMGGLALVLLAIMLMPRFGHWHQEPSTH

Sequences:

>Translated_400_residues
MQRNLLPILMSLVLLSPLAIDIYLPSMPAMALDFSVDERQVQSTLVLFLFAMGVGQVLIGPLADRYGRRPVALFGTVLYV
ISSLLAMAALEFELLQLARLLQGLAACSTSIVAFSAVRDSYSSADSARIYSYLNGAICVIPALAPTLGGLLAIQFGWRST
FAFMAFYGLVMLLVVGYRLPETRPAHTESAGPLYRWGRYAPVLKNSHFMFYAVTCMAAMAAILCYVSYSSIWLIGKLGVS
ELAFSGLFGLNAVVNVVACFAAPVVIRKLGNRPTVLVALGLMILASVLELVLQLPAWASPMAASFAFMLPMMLLCVGFAF
LLGPATSMALAPFGERAGTATALLGFIQMSGASVITALVQLTPMTAPYAIGYTMGGLALVLLAIMLMPRFGHWHQEPSTH
>Mature_400_residues
MQRNLLPILMSLVLLSPLAIDIYLPSMPAMALDFSVDERQVQSTLVLFLFAMGVGQVLIGPLADRYGRRPVALFGTVLYV
ISSLLAMAALEFELLQLARLLQGLAACSTSIVAFSAVRDSYSSADSARIYSYLNGAICVIPALAPTLGGLLAIQFGWRST
FAFMAFYGLVMLLVVGYRLPETRPAHTESAGPLYRWGRYAPVLKNSHFMFYAVTCMAAMAAILCYVSYSSIWLIGKLGVS
ELAFSGLFGLNAVVNVVACFAAPVVIRKLGNRPTVLVALGLMILASVLELVLQLPAWASPMAASFAFMLPMMLLCVGFAF
LLGPATSMALAPFGERAGTATALLGFIQMSGASVITALVQLTPMTAPYAIGYTMGGLALVLLAIMLMPRFGHWHQEPSTH

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=344, Percent_Identity=33.7209302325581, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI87082312, Length=349, Percent_Identity=30.0859598853868, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788509, Length=371, Percent_Identity=26.1455525606469, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI48994889, Length=358, Percent_Identity=27.3743016759777, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790794, Length=281, Percent_Identity=26.6903914590747, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787065, Length=334, Percent_Identity=26.9461077844311, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324264, Length=179, Percent_Identity=26.8156424581006, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42888; Mature: 42888

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRNLLPILMSLVLLSPLAIDIYLPSMPAMALDFSVDERQVQSTLVLFLFAMGVGQVLIG
CCCCHHHHHHHHHHHCCHHEEEECCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PLADRYGRRPVALFGTVLYVISSLLAMAALEFELLQLARLLQGLAACSTSIVAFSAVRDS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSSADSARIYSYLNGAICVIPALAPTLGGLLAIQFGWRSTFAFMAFYGLVMLLVVGYRLP
HCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHCCCC
ETRPAHTESAGPLYRWGRYAPVLKNSHFMFYAVTCMAAMAAILCYVSYSSIWLIGKLGVS
CCCCCCCCCCCCHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCHH
ELAFSGLFGLNAVVNVVACFAAPVVIRKLGNRPTVLVALGLMILASVLELVLQLPAWASP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
MAASFAFMLPMMLLCVGFAFLLGPATSMALAPFGERAGTATALLGFIQMSGASVITALVQ
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
LTPMTAPYAIGYTMGGLALVLLAIMLMPRFGHWHQEPSTH
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQRNLLPILMSLVLLSPLAIDIYLPSMPAMALDFSVDERQVQSTLVLFLFAMGVGQVLIG
CCCCHHHHHHHHHHHCCHHEEEECCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PLADRYGRRPVALFGTVLYVISSLLAMAALEFELLQLARLLQGLAACSTSIVAFSAVRDS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSSADSARIYSYLNGAICVIPALAPTLGGLLAIQFGWRSTFAFMAFYGLVMLLVVGYRLP
HCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHCCCC
ETRPAHTESAGPLYRWGRYAPVLKNSHFMFYAVTCMAAMAAILCYVSYSSIWLIGKLGVS
CCCCCCCCCCCCHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCHH
ELAFSGLFGLNAVVNVVACFAAPVVIRKLGNRPTVLVALGLMILASVLELVLQLPAWASP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
MAASFAFMLPMMLLCVGFAFLLGPATSMALAPFGERAGTATALLGFIQMSGASVITALVQ
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
LTPMTAPYAIGYTMGGLALVLLAIMLMPRFGHWHQEPSTH
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA