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The map label for this gene is ilvG [H]

Identifier: 119776413

GI number: 119776413

Start: 3896911

End: 3898581

Strand: Reverse

Name: ilvG [H]

Synonym: Sama_3281

Alternate gene names: 119776413

Gene position: 3898581-3896911 (Counterclockwise)

Preceding gene: 119776414

Following gene: 119776412

Centisome position: 90.54

GC content: 59.13

Gene sequence:

>1671_bases
ATGGAAGGACAGACGATGAGAGGGGCTGACGCAGTCATCAAGGTATTGGCCGCCCATGGGGTGAACACGGTATTCGGTTA
TCCCGGTGGCGCCATCATGCCCATCTACGACGCGCTTTACGGCAGTGAAGTCGAGCACACCCTGTGCCGCCACGAACAGG
GTGCCGGCTTTGCTGCCGTGGGCTATGCCCGTGCCAGTGGCAAAACCGGCGTCTGCTTCGCCACCTCTGGCCCCGGCGCC
ACCAACCTCATCACCGCCCTTGCCGATGCTCTGCTGGATTCAGTGCCCGTGGTGGCCATCACGGGCCAGGTGTCCACCTC
GGTTATCGGCACCGATGCCTTTCAGGAAATCGATGTACTTGGTATGAGCCTGTCCTGCACCAAGCACAGCTTTATGGTGC
AAACAGTCGATGAGCTGGTCCCCACGCTGTATCGGGCCTTTGAGCTGGCGGCCTCCGGCCGACCCGGGCCTGTGCTGGTT
GACATCCCCAAAGATATTCAAATAGCCAAACTCGAATACAGAGCGCCGCTGTTGGCCGTTGCAGATGAACCCAAAGTTCA
GGACGCCGACATCGATGCCGCCCGCGCCCTGATAGCGGCCGCCAAAAAGCCCATGTTGTACGTAGGCGGTGGTGTGGGTA
TGGCCGGCGCTGTCGAGCCGCTGCGCCATTTTATCAAGGCCACCTATATGCCGTCCGTGGCTACCCTCAAGGGCCTTGGT
GCTATCCCCCATGGCACACCTGGCTACCTGGGCATGCTGGGGATGCACGGCGGCAAGGCCGCTAACCTTGCGGTGCAGGA
ATGTGATTTGCTGATGGTGGTTGGCGCCCGTTTCGATGACCGCGTTACCGGCCGTTTGGCCTCATTTGCGCCCAATGCAA
AAGTGCTGCATCTGGATATAGATGCCGCCGAGCTTGGCAAGCTGCGTCAGCCCGATGTGGCCATTGCCGCCGAGCTGCGT
GTGGTGTTGCCAATGCTTGAGATGCAGCTTGATATAGACCCTTGGCGCGCTGAGGTCGAAGCGCTCGCCGCCGAACACCG
CTGGGATTACAACCACCCCGGCAGCCTCATCTACGCCCCTGCCATGCTGCGCCGTTTGGCCAACAAGTTGCCCGAGGACA
GCGTAGTGAGCTGCGATGTGGGCCAGCACCAGATGTGGGTTGCTCAGCATATGCACTTCCGCCGCCCTGAAGATCATTTG
TCCAGTGCAGGTCTTGGCACCATGGGTTTTGGCTTGCCAGCTGCCATAGGCGCTCAGATGGCGCGCCCCGATGCCACCGT
GGTGGCTGTGTCAGGCGATGGCTCTTTCATGATGAATGTGCAGGAACTGACTACCATCAAACGCCGCAAGCTGCCGGTGA
AAATCCTGCTTATCGACAACCAGCGTTTGGGCATGGTGAAGCAGTGGCAACAGCTGTTTTTTGAAGAGCGTTACAGCGAA
ACCAACCTGTCAGACAACCCGGATTTTGTTGCGCTGGCGTCGGCCTTTGATATCCCCGGGCGCACCATTTTTGCCGCCGA
AGAAGTGGAAGAAGCCCTGACCGAAATGCTCACCAGTAAGGGGCCTTACCTCTTGCACGTGGCCATAGATGATGCCTTTA
ACGTTTGGCCCCTGGTTCCGCCGGGTGCCTCCAACAGCGATATGATGGAAGAAATGGAGAGAAGCACATGA

Upstream 100 bases:

>100_bases
GAAAGAAGTTGCAGAAAAGCCCCCCGCTCCGACAGGACCGGGGGGTTTCTCGTTTCAGGGCATTAAAAAACTAGCGTACA
GGCGCAGGGCAGGAAATCAG

Downstream 100 bases:

>100_bases
ACCATAATCTGGAAGTCACCATGGCACAGCGTCCCGAAGTGGTTGAGCGTGTGCTGAGGGTTGTTCGCCACAGGGGATTT
ACCCTGACCAACATGAATAT

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MEGQTMRGADAVIKVLAAHGVNTVFGYPGGAIMPIYDALYGSEVEHTLCRHEQGAGFAAVGYARASGKTGVCFATSGPGA
TNLITALADALLDSVPVVAITGQVSTSVIGTDAFQEIDVLGMSLSCTKHSFMVQTVDELVPTLYRAFELAASGRPGPVLV
DIPKDIQIAKLEYRAPLLAVADEPKVQDADIDAARALIAAAKKPMLYVGGGVGMAGAVEPLRHFIKATYMPSVATLKGLG
AIPHGTPGYLGMLGMHGGKAANLAVQECDLLMVVGARFDDRVTGRLASFAPNAKVLHLDIDAAELGKLRQPDVAIAAELR
VVLPMLEMQLDIDPWRAEVEALAAEHRWDYNHPGSLIYAPAMLRRLANKLPEDSVVSCDVGQHQMWVAQHMHFRRPEDHL
SSAGLGTMGFGLPAAIGAQMARPDATVVAVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQRLGMVKQWQQLFFEERYSE
TNLSDNPDFVALASAFDIPGRTIFAAEEVEEALTEMLTSKGPYLLHVAIDDAFNVWPLVPPGASNSDMMEEMERST

Sequences:

>Translated_556_residues
MEGQTMRGADAVIKVLAAHGVNTVFGYPGGAIMPIYDALYGSEVEHTLCRHEQGAGFAAVGYARASGKTGVCFATSGPGA
TNLITALADALLDSVPVVAITGQVSTSVIGTDAFQEIDVLGMSLSCTKHSFMVQTVDELVPTLYRAFELAASGRPGPVLV
DIPKDIQIAKLEYRAPLLAVADEPKVQDADIDAARALIAAAKKPMLYVGGGVGMAGAVEPLRHFIKATYMPSVATLKGLG
AIPHGTPGYLGMLGMHGGKAANLAVQECDLLMVVGARFDDRVTGRLASFAPNAKVLHLDIDAAELGKLRQPDVAIAAELR
VVLPMLEMQLDIDPWRAEVEALAAEHRWDYNHPGSLIYAPAMLRRLANKLPEDSVVSCDVGQHQMWVAQHMHFRRPEDHL
SSAGLGTMGFGLPAAIGAQMARPDATVVAVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQRLGMVKQWQQLFFEERYSE
TNLSDNPDFVALASAFDIPGRTIFAAEEVEEALTEMLTSKGPYLLHVAIDDAFNVWPLVPPGASNSDMMEEMERST
>Mature_556_residues
MEGQTMRGADAVIKVLAAHGVNTVFGYPGGAIMPIYDALYGSEVEHTLCRHEQGAGFAAVGYARASGKTGVCFATSGPGA
TNLITALADALLDSVPVVAITGQVSTSVIGTDAFQEIDVLGMSLSCTKHSFMVQTVDELVPTLYRAFELAASGRPGPVLV
DIPKDIQIAKLEYRAPLLAVADEPKVQDADIDAARALIAAAKKPMLYVGGGVGMAGAVEPLRHFIKATYMPSVATLKGLG
AIPHGTPGYLGMLGMHGGKAANLAVQECDLLMVVGARFDDRVTGRLASFAPNAKVLHLDIDAAELGKLRQPDVAIAAELR
VVLPMLEMQLDIDPWRAEVEALAAEHRWDYNHPGSLIYAPAMLRRLANKLPEDSVVSCDVGQHQMWVAQHMHFRRPEDHL
SSAGLGTMGFGLPAAIGAQMARPDATVVAVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQRLGMVKQWQQLFFEERYSE
TNLSDNPDFVALASAFDIPGRTIFAAEEVEEALTEMLTSKGPYLLHVAIDDAFNVWPLVPPGASNSDMMEEMERST

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.7349823321555, Blast_Score=156, Evalue=6e-38,
Organism=Homo sapiens, GI21361361, Length=518, Percent_Identity=26.4478764478764, Blast_Score=143, Evalue=5e-34,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=46.0980036297641, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=42.7574171029668, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=30.8377896613191, Blast_Score=255, Evalue=7e-69,
Organism=Escherichia coli, GI1787096, Length=543, Percent_Identity=27.9926335174954, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=25.7194244604317, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=490, Percent_Identity=27.9591836734694, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=490, Percent_Identity=27.9591836734694, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=532, Percent_Identity=26.8796992481203, Blast_Score=125, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=43.0769230769231, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320588, Length=581, Percent_Identity=21.1703958691911, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320816, Length=485, Percent_Identity=22.680412371134, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=510, Percent_Identity=23.3333333333333, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=465, Percent_Identity=24.0860215053763, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=481, Percent_Identity=23.2848232848233, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=499, Percent_Identity=27.8557114228457, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59640; Mature: 59640

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGQTMRGADAVIKVLAAHGVNTVFGYPGGAIMPIYDALYGSEVEHTLCRHEQGAGFAAV
CCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEE
GYARASGKTGVCFATSGPGATNLITALADALLDSVPVVAITGQVSTSVIGTDAFQEIDVL
EEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCHHHHHHHHH
GMSLSCTKHSFMVQTVDELVPTLYRAFELAASGRPGPVLVDIPKDIQIAKLEYRAPLLAV
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEECCCEEEE
ADEPKVQDADIDAARALIAAAKKPMLYVGGGVGMAGAVEPLRHFIKATYMPSVATLKGLG
CCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHCC
AIPHGTPGYLGMLGMHGGKAANLAVQECDLLMVVGARFDDRVTGRLASFAPNAKVLHLDI
CCCCCCCCHHEEEECCCCCCCCEEHHHCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEC
DAAELGKLRQPDVAIAAELRVVLPMLEMQLDIDPWRAEVEALAAEHRWDYNHPGSLIYAP
CHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEHH
AMLRRLANKLPEDSVVSCDVGQHQMWVAQHMHFRRPEDHLSSAGLGTMGFGLPAAIGAQM
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHH
ARPDATVVAVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQRLGMVKQWQQLFFEERYSE
CCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCC
TNLSDNPDFVALASAFDIPGRTIFAAEEVEEALTEMLTSKGPYLLHVAIDDAFNVWPLVP
CCCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
PGASNSDMMEEMERST
CCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEGQTMRGADAVIKVLAAHGVNTVFGYPGGAIMPIYDALYGSEVEHTLCRHEQGAGFAAV
CCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEE
GYARASGKTGVCFATSGPGATNLITALADALLDSVPVVAITGQVSTSVIGTDAFQEIDVL
EEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCHHHHHHHHH
GMSLSCTKHSFMVQTVDELVPTLYRAFELAASGRPGPVLVDIPKDIQIAKLEYRAPLLAV
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEECCCEEEE
ADEPKVQDADIDAARALIAAAKKPMLYVGGGVGMAGAVEPLRHFIKATYMPSVATLKGLG
CCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHCC
AIPHGTPGYLGMLGMHGGKAANLAVQECDLLMVVGARFDDRVTGRLASFAPNAKVLHLDI
CCCCCCCCHHEEEECCCCCCCCEEHHHCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEC
DAAELGKLRQPDVAIAAELRVVLPMLEMQLDIDPWRAEVEALAAEHRWDYNHPGSLIYAP
CHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEHH
AMLRRLANKLPEDSVVSCDVGQHQMWVAQHMHFRRPEDHLSSAGLGTMGFGLPAAIGAQM
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHH
ARPDATVVAVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQRLGMVKQWQQLFFEERYSE
CCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCC
TNLSDNPDFVALASAFDIPGRTIFAAEEVEEALTEMLTSKGPYLLHVAIDDAFNVWPLVP
CCCCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
PGASNSDMMEEMERST
CCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]