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The map label for this gene is ilvA [H]

Identifier: 119776410

GI number: 119776410

Start: 3893120

End: 3894715

Strand: Reverse

Name: ilvA [H]

Synonym: Sama_3278

Alternate gene names: 119776410

Gene position: 3894715-3893120 (Counterclockwise)

Preceding gene: 119776411

Following gene: 119776409

Centisome position: 90.45

GC content: 57.83

Gene sequence:

>1596_bases
ATGCTGGATTTTTCTGGCTGTGGTGACAGAGATAAATCAGAGGTGAAGCCTGTGGATCTTGCTCATTATTATTTGCAAAA
AATCCTGCTGTCGTCTGTGTATGACGTGGCCAAGGTGACGCCCCTGTCCCATATGGGCAAGCTGTCGGCGCGCCTGGGGC
AGGAGATTTATTTAAAGCGTGAAGACATGCAGCCGGTGCATTCCTTTAAGCTGCGTGGCGCCTATAACCGCATTTCGCAG
CTGACTCAGCCTGAGTGCGAGCGCGGTGTGGTATGCGCTTCGGCGGGTAACCATGCTCAGGGCGTTGCGTTGTCGGCCTC
AAGCCGTGGCATTGATGCAGTGATAGTCATGCCCACCACCACGCCGGATATCAAAATCGATGCGGTGCGTCGTCGTGGCG
GCAATGTGCTCTTGCACGGTGAGTCCTTCGATCAGGCCAACGCCCACGCTCAGCATCTGGCGCAAACCGAGGGCCGGGTC
TATATCGCGCCTTTCGATGATGAGGCTGTGATTGCCGGGCAGGGCACAGTGGCGCAGGAAATGCTGCAGCAGCAGCGGGA
TCTGGAGCTGGTATTTGTGCCCGTGGGCGGTGGTGGCCTGGTGGCCGGCATCGCTGCCTATTACAAGGCGGTGCGACCTT
CGGTGAAAATCATCGGGGTCGAGCCTGAGGATGCCGCTTGCCTTAAGGCCGCCATGGCCGCGGGTGAACCTGTGACTTTA
TCCCAGGTGGGCCTCTTTGCCGATGGCGTAGCGGTAAAGCGCATCGGCGCTGAGCCATTTCGTATCGCCAAACACTATGT
GGATGAAGTGGTGACCGTCAGCTCAGACGAAATCTGCGCCGCGGTGAAGGATATCTTCGAAGACACCCGTGCCATTGCTG
AGCCGGCGGGCGCGCTGTCTCTGGCGGGGCTGAAAAAGTATCTGGCGCAGACAACACCTGCTCTTGCTGAGGCGGGCAAA
CCAGCCCGCAAGGTGGCGGCCATTTTGAGTGGCGCCAACGTGAATTTTCATAGCCTGAGATACGTGTCTGAGCGCTGCGA
ACTGGGTGAGCAAAAGGAAGCTGTGCTGGCGGTGAAGGTGCCGGAAGTGCCCGGTAGCTTTCTTAAGTTTTGCGAGCTTT
TGGGCAAGCGCGCCATGACTGAATTCAACTACCGCTTCTCGGGCCGCGACAGCGCCGTGGTGTTTGCCGGTATCCGGCTC
TCCGGTGGCCAGTCGGAGCTTAAAGAGATCATCGCGTCCCTCACTTCAGGCGGCTTTGAAGTGCAGGATTTATCGAGCGA
TGAAACCGCCAAACTGCATGTGCGTTATATGGTGGGAGGTCACCCCACAGAGCCCTTGAGTGAGCGGTTATTCAGCTTCG
AGTTTCCCGAGTATCCGGGGGCACTGCTTAAGTTTTTAACCACCCTCGGCAGCCGCTGGAACATCAGTTTGTTCCACTAC
CGCAACCACGGCGCTGCCTTTGGTCGGGTTCTGGCGGGATTCGAGGTGCCGGAGGGCGACAACGAGGCTTTTGGCCGCTT
CCTCACCGAGCTTGGCTTTGTGTGGCAGGAAGAAACCCACAGCCCGGCTTACCGCTTGTTTTTGGGCAGTAAATAG

Upstream 100 bases:

>100_bases
CGGGTTCGCCCTGTGTCGATGGCGCTGAAGGCGTATGCCATGCTCGCCACCAGTGCCGATAAGGGCGCGGTGCGTGATGT
CTCCAAACTGGAGGGCTGAG

Downstream 100 bases:

>100_bases
ATATGACGCGCCCCTCCCTTAGACCAAGGCAGGGGCGTTTTAGTATCAGGAATATGATAGGGTAAATGCTCGAAATGATG
AGATAACCCAGACCTCAGCG

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MLDFSGCGDRDKSEVKPVDLAHYYLQKILLSSVYDVAKVTPLSHMGKLSARLGQEIYLKREDMQPVHSFKLRGAYNRISQ
LTQPECERGVVCASAGNHAQGVALSASSRGIDAVIVMPTTTPDIKIDAVRRRGGNVLLHGESFDQANAHAQHLAQTEGRV
YIAPFDDEAVIAGQGTVAQEMLQQQRDLELVFVPVGGGGLVAGIAAYYKAVRPSVKIIGVEPEDAACLKAAMAAGEPVTL
SQVGLFADGVAVKRIGAEPFRIAKHYVDEVVTVSSDEICAAVKDIFEDTRAIAEPAGALSLAGLKKYLAQTTPALAEAGK
PARKVAAILSGANVNFHSLRYVSERCELGEQKEAVLAVKVPEVPGSFLKFCELLGKRAMTEFNYRFSGRDSAVVFAGIRL
SGGQSELKEIIASLTSGGFEVQDLSSDETAKLHVRYMVGGHPTEPLSERLFSFEFPEYPGALLKFLTTLGSRWNISLFHY
RNHGAAFGRVLAGFEVPEGDNEAFGRFLTELGFVWQEETHSPAYRLFLGSK

Sequences:

>Translated_531_residues
MLDFSGCGDRDKSEVKPVDLAHYYLQKILLSSVYDVAKVTPLSHMGKLSARLGQEIYLKREDMQPVHSFKLRGAYNRISQ
LTQPECERGVVCASAGNHAQGVALSASSRGIDAVIVMPTTTPDIKIDAVRRRGGNVLLHGESFDQANAHAQHLAQTEGRV
YIAPFDDEAVIAGQGTVAQEMLQQQRDLELVFVPVGGGGLVAGIAAYYKAVRPSVKIIGVEPEDAACLKAAMAAGEPVTL
SQVGLFADGVAVKRIGAEPFRIAKHYVDEVVTVSSDEICAAVKDIFEDTRAIAEPAGALSLAGLKKYLAQTTPALAEAGK
PARKVAAILSGANVNFHSLRYVSERCELGEQKEAVLAVKVPEVPGSFLKFCELLGKRAMTEFNYRFSGRDSAVVFAGIRL
SGGQSELKEIIASLTSGGFEVQDLSSDETAKLHVRYMVGGHPTEPLSERLFSFEFPEYPGALLKFLTTLGSRWNISLFHY
RNHGAAFGRVLAGFEVPEGDNEAFGRFLTELGFVWQEETHSPAYRLFLGSK
>Mature_531_residues
MLDFSGCGDRDKSEVKPVDLAHYYLQKILLSSVYDVAKVTPLSHMGKLSARLGQEIYLKREDMQPVHSFKLRGAYNRISQ
LTQPECERGVVCASAGNHAQGVALSASSRGIDAVIVMPTTTPDIKIDAVRRRGGNVLLHGESFDQANAHAQHLAQTEGRV
YIAPFDDEAVIAGQGTVAQEMLQQQRDLELVFVPVGGGGLVAGIAAYYKAVRPSVKIIGVEPEDAACLKAAMAAGEPVTL
SQVGLFADGVAVKRIGAEPFRIAKHYVDEVVTVSSDEICAAVKDIFEDTRAIAEPAGALSLAGLKKYLAQTTPALAEAGK
PARKVAAILSGANVNFHSLRYVSERCELGEQKEAVLAVKVPEVPGSFLKFCELLGKRAMTEFNYRFSGRDSAVVFAGIRL
SGGQSELKEIIASLTSGGFEVQDLSSDETAKLHVRYMVGGHPTEPLSERLFSFEFPEYPGALLKFLTTLGSRWNISLFHY
RNHGAAFGRVLAGFEVPEGDNEAFGRFLTELGFVWQEETHSPAYRLFLGSK

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=314, Percent_Identity=33.7579617834395, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI33469958, Length=310, Percent_Identity=28.7096774193548, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI19923959, Length=314, Percent_Identity=30.5732484076433, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1790207, Length=506, Percent_Identity=60.8695652173913, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1789505, Length=301, Percent_Identity=38.8704318936877, Blast_Score=188, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI71991565, Length=328, Percent_Identity=35.3658536585366, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17537387, Length=363, Percent_Identity=31.404958677686, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17508781, Length=287, Percent_Identity=28.9198606271777, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320930, Length=516, Percent_Identity=50, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322631, Length=304, Percent_Identity=35.1973684210526, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6319788, Length=294, Percent_Identity=25.5102040816327, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322024, Length=161, Percent_Identity=30.4347826086957, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI21355833, Length=322, Percent_Identity=33.8509316770186, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24645328, Length=258, Percent_Identity=32.5581395348837, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 57495; Mature: 57495

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDFSGCGDRDKSEVKPVDLAHYYLQKILLSSVYDVAKVTPLSHMGKLSARLGQEIYLKR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHEEEC
EDMQPVHSFKLRGAYNRISQLTQPECERGVVCASAGNHAQGVALSASSRGIDAVIVMPTT
CCCCHHHHHHHHHHHHHHHHCCCCCHHCCEEEECCCCCCCCEEEECCCCCCEEEEEEECC
TPDIKIDAVRRRGGNVLLHGESFDQANAHAQHLAQTEGRVYIAPFDDEAVIAGQGTVAQE
CCCEEEEHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHH
MLQQQRDLELVFVPVGGGGLVAGIAAYYKAVRPSVKIIGVEPEDAACLKAAMAAGEPVTL
HHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEH
SQVGLFADGVAVKRIGAEPFRIAKHYVDEVVTVSSDEICAAVKDIFEDTRAIAEPAGALS
HHHHHHHCCHHHHHCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCCCHHH
LAGLKKYLAQTTPALAEAGKPARKVAAILSGANVNFHSLRYVSERCELGEQKEAVLAVKV
HHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEC
PEVPGSFLKFCELLGKRAMTEFNYRFSGRDSAVVFAGIRLSGGQSELKEIIASLTSGGFE
CCCCHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEE
VQDLSSDETAKLHVRYMVGGHPTEPLSERLFSFEFPEYPGALLKFLTTLGSRWNISLFHY
EEECCCCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEE
RNHGAAFGRVLAGFEVPEGDNEAFGRFLTELGFVWQEETHSPAYRLFLGSK
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MLDFSGCGDRDKSEVKPVDLAHYYLQKILLSSVYDVAKVTPLSHMGKLSARLGQEIYLKR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHEEEC
EDMQPVHSFKLRGAYNRISQLTQPECERGVVCASAGNHAQGVALSASSRGIDAVIVMPTT
CCCCHHHHHHHHHHHHHHHHCCCCCHHCCEEEECCCCCCCCEEEECCCCCCEEEEEEECC
TPDIKIDAVRRRGGNVLLHGESFDQANAHAQHLAQTEGRVYIAPFDDEAVIAGQGTVAQE
CCCEEEEHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHH
MLQQQRDLELVFVPVGGGGLVAGIAAYYKAVRPSVKIIGVEPEDAACLKAAMAAGEPVTL
HHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEH
SQVGLFADGVAVKRIGAEPFRIAKHYVDEVVTVSSDEICAAVKDIFEDTRAIAEPAGALS
HHHHHHHCCHHHHHCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCCCHHH
LAGLKKYLAQTTPALAEAGKPARKVAAILSGANVNFHSLRYVSERCELGEQKEAVLAVKV
HHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEC
PEVPGSFLKFCELLGKRAMTEFNYRFSGRDSAVVFAGIRLSGGQSELKEIIASLTSGGFE
CCCCHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEE
VQDLSSDETAKLHVRYMVGGHPTEPLSERLFSFEFPEYPGALLKFLTTLGSRWNISLFHY
EEECCCCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEE
RNHGAAFGRVLAGFEVPEGDNEAFGRFLTELGFVWQEETHSPAYRLFLGSK
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]